Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	65	25.3	1027584	98.0%	1007032	120.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,489	G→A	G475G (GGC→GGT)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,489	0	G	A	100.0%	60.3 / NA	18	G475G (GGC→GGT)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/10); total (8/10)

TGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACT  >  NC_000913/1629370‑1629617

tgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACa                                                                                                               >  1:445079/1‑139 (MQ=255)

      gaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACg                                                                                                         >  2:310789/1‑139 (MQ=255)

       aaaCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGt                                                                                                        >  2:462942/1‑139 (MQ=255)

               accacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCa                                                                                                <  2:103488/139‑1 (MQ=255)

                      gACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGc                                                                                         <  1:36797/139‑1 (MQ=255)

                      gACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGc                                                                                         <  2:186338/139‑1 (MQ=255)

                                     ggCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCtaata                                                                                                                     <  1:293555/96‑1 (MQ=255)

                                     ggCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCtaata                                                                                                                     >  2:293555/1‑96 (MQ=255)

                                       cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGt                                                                        <  1:310789/139‑1 (MQ=255)

                                               aaGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCaaa                                                                >  2:40673/1‑139 (MQ=255)

                                                 gCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatc                                                              >  1:68283/1‑139 (MQ=255)

                                                                                      cTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTgg                         >  2:58300/1‑139 (MQ=255)

                                                                                                        cACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaa                                                                                   <  1:7346/63‑1 (MQ=255)

                                                                                                        cACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaa                                                                                   >  2:7346/1‑63 (MQ=255)

                                                                                                           ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa    <  2:201289/139‑1 (MQ=255)

                                                                                                           ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa    <  2:445079/139‑1 (MQ=255)

                                                                                                           ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa    <  1:40673/139‑1 (MQ=255)

                                                                                                             tCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCact  <  1:58300/139‑1 (MQ=255)

TGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACT  >  NC_000913/1629370‑1629617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A