Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
69 |
53.0 |
2252012 |
96.1% |
2164183 |
115.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,827,934 |
G→A |
A109V (GCC→GTC) |
astB ← |
succinylarginine dihydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,827,934 | 0 | G | A | 100.0%
| 89.6
/ NA
| 27 | A109V (GCC→GTC) | astB | succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/11); total (16/11) |
AGCGATTCAGTGACGGGCGCTTCCA‑‑GCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGA > NC_000913/1827817‑1828059
|
aGCGATTCAGTGACGGGCGCTTCCA‑‑GCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCgg > 1:1083935/1‑139 (MQ=255)
cGATTCAGTGACGGGCGCTTCCAGCGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCg > 2:960574/1‑133 (MQ=255)
cGATTCAGTGACGGGCGCTTCCAGCGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCg < 1:960574/133‑1 (MQ=255)
ggCGCTTCCA‑‑GCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCAGATTgg > 1:673264/1‑116 (MQ=255)
ggCGCTTCCA‑‑GCGAACGGCGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTgg < 2:673264/116‑1 (MQ=255)
ggCGCTTCCA‑‑GCGAACGGCGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGaaa > 1:19018/1‑139 (MQ=255)
cGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCt > 2:253574/1‑139 (MQ=255)
gTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACTCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTgt > 2:857080/1‑138 (MQ=255)
gTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGAc < 1:308912/114‑1 (MQ=255)
gTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGAc > 2:308912/1‑114 (MQ=255)
tttATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCa < 2:319309/139‑1 (MQ=255)
tctcaccgTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACttt > 2:846397/8‑139 (MQ=255)
tCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTc < 1:568110/139‑1 (MQ=255)
tCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTc < 2:463030/139‑1 (MQ=255)
cAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGACGTGCAACTTTTTcc > 1:368618/1‑139 (MQ=255)
cAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTg > 2:24189/1‑120 (MQ=255)
cAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTg < 1:24189/120‑1 (MQ=255)
agaTGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGc < 1:253574/139‑1 (MQ=255)
aCTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCt < 2:191855/100‑1 (MQ=255)
aCTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCt > 1:191855/1‑100 (MQ=255)
tCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCa > 2:354336/1‑139 (MQ=255)
ccAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCaa > 1:841614/1‑139 (MQ=255)
cGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGa > 2:367566/1‑139 (MQ=255)
ggCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGaa > 2:404169/1‑139 (MQ=255)
cGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGTAATAAACGGa < 1:857080/139‑1 (MQ=255)
aTTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACt < 2:805893/67‑1 (MQ=255)
aTTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACt > 1:805893/1‑67 (MQ=255)
|
AGCGATTCAGTGACGGGCGCTTCCA‑‑GCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGA > NC_000913/1827817‑1828059
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
