Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
65 |
25.3 |
1027584 |
98.0% |
1007032 |
120.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,827,934 |
G→A |
A109V (GCC→GTC) |
astB ← |
succinylarginine dihydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,827,934 | 0 | G | A | 100.0%
| 40.8
/ NA
| 13 | A109V (GCC→GTC) | astB | succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/8); total (5/8) |
TTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACG > NC_000913/1827822‑1828040
|
ttCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTg < 1:135619/139‑1 (MQ=255)
cGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGa < 2:452118/139‑1 (MQ=255)
gcTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGcc > 2:40988/1‑139 (MQ=255)
ttCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACttttt < 1:326463/139‑1 (MQ=255)
agaTGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACt < 1:124424/117‑1 (MQ=255)
agaTGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACt > 2:124424/1‑117 (MQ=255)
cACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCAt < 1:96380/131‑1 (MQ=255)
cACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCAt > 2:96380/1‑131 (MQ=255)
cTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACt < 1:40988/139‑1 (MQ=255)
tGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTg < 1:216768/88‑1 (MQ=255)
tGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTg > 2:216768/1‑88 (MQ=255)
cGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGCCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTTCCACTGAATCCCAACTGa > 1:434526/1‑139 (MQ=255)
tATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACg < 2:429302/139‑1 (MQ=255)
|
TTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACG > NC_000913/1827822‑1828040
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
