Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	69	53.0	2252012	96.1%	2164183	115.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,459,283	G→A	A396V (GCG→GTG)	fadI ←	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,459,283	0	G	A	100.0%	129.9 / NA	37	A396V (GCG→GTG)	fadI	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/20); total (17/20)

GTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGA  >  NC_000913/2459149‑2459408

gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcaccgc                                                                                                                           <  2:752082/139‑1 (MQ=255)

gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcaccgc                                                                                                                           <  2:841962/139‑1 (MQ=255)

       ccATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGa                                                                                                                    <  1:682938/139‑1 (MQ=255)

       ccATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGa                                                                                                                    <  2:973190/139‑1 (MQ=255)

             ttCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCg                                                                                                              >  1:840689/1‑139 (MQ=255)

                 gCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAg                                                                                                          <  2:719297/139‑1 (MQ=255)

                  ccTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGc                                                                                                         >  1:896829/1‑139 (MQ=255)

                        gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGa                                                                                                   <  1:610589/139‑1 (MQ=255)

                                    cgcCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCAc                                                                                       >  2:1012691/1‑139 (MQ=255)

                                       cAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGtt                                                                                    <  1:413014/139‑1 (MQ=255)

                                       cAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGtt                                                                                    <  1:617/139‑1 (MQ=255)

                                       cAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGtt                                                                                    <  1:618117/139‑1 (MQ=255)

                                         aGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTaa                                                                                  >  2:6274/1‑139 (MQ=255)

                                                 ggCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTa                                                                          <  1:267541/139‑1 (MQ=255)

                                                         aGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCAc                                                                  <  2:48431/139‑1 (MQ=255)

                                                                aaCTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCa                                                           >  2:193870/1‑139 (MQ=255)

                                                                  cTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGt                                                         <  1:325999/139‑1 (MQ=255)

                                                                     aaCCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                      <  1:1012691/139‑1 (MQ=255)

                                                                           aTCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    <  2:623772/87‑1 (MQ=255)

                                                                           aTCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    >  1:623772/1‑87 (MQ=255)

                                                                           aTCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGc                                                >  1:660981/1‑139 (MQ=255)

                                                                                    cgcgACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAg                                       >  1:306064/1‑139 (MQ=255)

                                                                                      cgACGGCAAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTg                                     >  2:736207/1‑139 (MQ=255)

                                                                                           gCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCa                                >  2:122382/1‑139 (MQ=255)

                                                                                                     tGCAATGTCTGGGTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAAc                      <  1:59549/139‑1 (MQ=255)

                                                                                                                ggTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTAccc           <  2:325501/139‑1 (MQ=255)

                                                                                                                 gTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                      <  1:566188/95‑1 (MQ=255)

                                                                                                                 gTAATCATCCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGc                                                      >  2:566188/1‑95 (MQ=255)

                                                                                                                    atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    >  2:467629/1‑46 (MQ=255)

                                                                                                                    atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCg                                                                                                    <  1:467629/46‑1 (MQ=255)

                                                                                                                    atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCa       <  1:122382/139‑1 (MQ=255)

                                                                                                                    atcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCa       >  2:189547/1‑139 (MQ=255)

                                                                                                                     tcatcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCaa      >  2:444597/1‑139 (MQ=255)

                                                                                                                      catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGaa                                                                                                  <  2:589102/46‑1 (MQ=255)

                                                                                                                      catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGaa                                                                                                  >  1:589102/1‑46 (MQ=255)

                                                                                                                      catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGc        >  2:505167/1‑136 (MQ=255)

                                                                                                                      catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGc        <  1:505167/136‑1 (MQ=255)

                                                                                                                      catcCGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAAc     >  1:968110/1‑139 (MQ=255)

                                                                                                                         ccGCGCGCCGGTCACCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGa  <  2:925672/139‑1 (MQ=255)

GTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGA  >  NC_000913/2459149‑2459408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A