Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 69 53.0 2252012 96.1% 2164183 115.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,284,931 A→G T83A (ACC→GCC)  agaC → N‑acetylgalactosamine‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,284,9310AG100.0% 126.0 / NA 38T83A (ACC→GCC) agaCN‑acetylgalactosamine‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/24);  total (14/24)

CTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCACCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCA  >  NC_000913/3284799‑3285054
                                                                                                                                    |                                                                                                                           
cTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCa                                                                                                                       >  1:1016460/1‑139 (MQ=255)
 tAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCAt                                                                                                                      >  1:904326/1‑139 (MQ=255)
  aaCTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCAtt                                                                                                                     >  1:1031136/1‑139 (MQ=255)
   aCTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCCGATGACCGACGTccatg                                                                                                                    >  2:988432/1‑135 (MQ=255)
     tGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCa                                                                                                                  <  1:885946/139‑1 (MQ=255)
     tGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCa                                                                                                                  <  1:783553/139‑1 (MQ=255)
      ggCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCa                                                                                                                  >  2:933258/1‑138 (MQ=255)
      ggCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCa                                                                                                                  <  1:933258/138‑1 (MQ=255)
      ggCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATAACCGCCGTccctgcct                                                                                                                 >  2:21560/1‑132 (MQ=255)
        cgcTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTTCATgg                                                                                                               >  1:736679/1‑139 (MQ=255)
             ttCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGATGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCCTGGGCTAc                                                                                                          >  2:687507/1‑139 (MQ=255)
                         ttCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCa                                                                                                                  <  1:543746/119‑1 (MQ=255)
                         ttCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCa                                                                                                                  >  2:543746/1‑119 (MQ=255)
                              aCTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCaaaa                                                                                         <  2:904326/139‑1 (MQ=255)
                                               tggtCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGc                                                                        <  1:897193/139‑1 (MQ=255)
                                               aggtCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGc                                                                        <  1:263771/138‑1 (MQ=255)
                                                 gtCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCt                                                                      >  1:538409/1‑139 (MQ=255)
                                                 gtCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCt                                                                      >  1:916101/1‑139 (MQ=255)
                                                    tGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGcc                                                                   <  2:916101/139‑1 (MQ=255)
                                                          agTTGGCTTTCGCCTGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGTGTCTGATGACCGCCGTCATTGCATAGTCTACGGGCGTTGATGCCAAAACAGAAATTGGTCTTGGCCTGGCGTTTAg                                                             <  1:687507/139‑1 (MQ=255)
                                                          agTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAg                                                             <  2:874866/139‑1 (MQ=255)
                                                          agTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAg                                                             <  1:988432/139‑1 (MQ=255)
                                                          agTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAg                                                             <  2:727816/139‑1 (MQ=255)
                                                          agTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAg                                                             <  1:523459/139‑1 (MQ=255)
                                                            ttGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCCTTGCATGGTCTACGGGCGTTGATGCCCAAACAGCAATTGGTCTTTGCCTGCCGTTTAgct                                                           >  2:263771/1‑137 (MQ=255)
                                                               gCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGt                                                        <  2:261851/139‑1 (MQ=255)
                                                                    cGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGt                                                        <  2:132021/134‑1 (MQ=255)
                                                                    cGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAAAAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGt                                                        >  1:132021/1‑134 (MQ=255)
                                                                      agggATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCa                                                 <  1:564100/137‑1 (MQ=255)
                                                                         gATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTa                                              <  1:89991/139‑1 (MQ=255)
                                                                                cccTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCAtt                                       <  2:1031136/139‑1 (MQ=255)
                                                                                   tGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATTAACGCCGTCATTTCATGGTCTTAGGGCGGTGATGCCAAAAAAGCAATTTGGCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCAtttcc                                    >  2:564100/1‑136 (MQ=255)
                                                                                   tGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTg                                    <  2:538409/139‑1 (MQ=255)
                                                                                              ttCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGGCTTGGCCTGCCGTTTAGTTTGTTTATGCAGTACGTCATTCTGTTCTTCTATTc                         >  1:947240/1‑139 (MQ=255)
                                                                                                cAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCg                       <  2:736679/139‑1 (MQ=255)
                                                                                                   ccgccCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCCATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCtt                    >  1:586839/1‑139 (MQ=255)
                                                                                                      cccAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTtct                 <  1:479454/139‑1 (MQ=255)
                                                                                                        cAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCa               <  1:21560/139‑1 (MQ=255)
                                                                                                                     gCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCa  <  2:947240/139‑1 (MQ=255)
                                                                                                                            tgatgaCCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTgg                                                                         <  1:152509/61‑1 (MQ=255)
                                                                                                                            tgatgaCCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTgg                                                                         >  2:152509/1‑61 (MQ=255)
                                                                                                                                    |                                                                                                                           
CTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCACCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCA  >  NC_000913/3284799‑3285054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A