Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
65 |
25.3 |
1027584 |
98.0% |
1007032 |
120.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,284,931 |
A→G |
T83A (ACC→GCC) |
agaC → |
N‑acetylgalactosamine‑specific enzyme IIC component of PTS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,284,931 | 0 | A | G | 100.0%
| 31.3
/ NA
| 12 | T83A (ACC→GCC) | agaC | N‑acetylgalactosamine‑specific enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/6); total (6/6) |
CTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCACCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCAAAGCC > NC_000913/3284799‑3285059
|
cTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCa > 2:99544/1‑139 (MQ=255)
tGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGtt < 1:99544/139‑1 (MQ=255)
tAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAAt < 1:77504/139‑1 (MQ=255)
cGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGtt < 1:413690/126‑1 (MQ=255)
cGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGtt > 2:413690/1‑126 (MQ=255)
ccTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTc < 2:55623/139‑1 (MQ=255)
gtgtTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGGCTTGGCCTGCCGTTTAGTTTGTTTATGCAGTACGTCATTCTGTTCTTCTa > 2:216262/1‑139 (MQ=255)
ccgccCAACCCGATTATGGCGGGTCTGATGACCGCCGTCATTGCACGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGGCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCtt > 2:158295/1‑139 (MQ=255)
gCGGGTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCa < 2:4321/139‑1 (MQ=255)
cGGGTCTGATGACCGCCGTCATTGCATGGTCTTCGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTTTTAATGCAGTACGTCATTTTGTTTTTCTATTCCGCTTTCTCATTATTTTTGACCaa > 1:4321/1‑139 (MQ=255)
gggTCTGATGACCGCCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCaaa < 2:445757/139‑1 (MQ=255)
tCTGATGACCGCCCTCCTTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTTATGCAGTACGTCCTTCTGTTCTTCTATTCCGCTTTCTCCTTATTTTTTACCAAAgac > 1:18183/1‑137 (MQ=255)
|
CTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCACCGTCATTGCATGGTCTACGGGCGTTGATGCCAAAACAGCAATTGGTCTTGGCCTGCCGTTTAGTTTGTTAATGCAGTACGTCATTCTGTTCTTCTATTCCGCTTTCTCATTATTTATGACCAAAGCC > NC_000913/3284799‑3285059
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
