Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,702,700	T→C	F156F (TTT→TTC)	add →	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,702,700	0	T	C	100.0%	133.4 / NA	36	F156F (TTT→TTC)	add	adenosine deaminase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/17); total (19/17)

TAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTTTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCG  >  NC_000913/1702567‑1702830

tAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               >  2:541167/1‑139 (MQ=255)

tAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               <  1:541167/139‑1 (MQ=255)

tAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               <  1:72979/139‑1 (MQ=255)

   cGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCa                                                                                                                            <  2:41723/139‑1 (MQ=255)

       tgttgtCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGt                                                                                                                        >  2:876668/1‑139 (MQ=255)

          tgtCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGAc                                                                                                                     <  1:236649/139‑1 (MQ=255)

          tgtCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGAc                                                                                                                     >  2:938196/1‑139 (MQ=255)

                   ggTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTAcc                                                                                                            >  2:635635/1‑139 (MQ=255)

                      gATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCa                                                                                                         >  2:336223/1‑139 (MQ=255)

                               cGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTa                                                                                                >  1:517551/1‑139 (MQ=255)

                                 tACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               >  1:231867/1‑106 (MQ=255)

                                 tACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGc                                                                                                                               <  2:231867/106‑1 (MQ=255)

                                 tACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGc                                                                                              >  2:63200/1‑139 (MQ=255)

                                   cGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCCGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCg                                                                                            >  1:301239/1‑139 (MQ=255)

                                              ccGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACtt                                                                                 >  1:1107731/1‑139 (MQ=255)

                                                        ggTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCgg                                                                       >  1:846391/1‑139 (MQ=255)

                                                        ggTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCgg                                                                       <  1:635635/139‑1 (MQ=255)

                                                          tgtgCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGa                                                                     <  1:876668/139‑1 (MQ=255)

                                                             gCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGt                                                                  <  2:243740/139‑1 (MQ=255)

                                                              cAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTc                                                                 >  2:506850/1‑139 (MQ=255)

                                                               aGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCt                                                                <  1:274071/139‑1 (MQ=255)

                                                                    aaGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTcc                                                           >  1:994673/1‑139 (MQ=255)

                                                                      gCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGtg                                                                                         <  2:1110240/107‑1 (MQ=255)

                                                                      gCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGtg                                                                                         >  1:1110240/1‑107 (MQ=255)

                                                                       cTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCttt                                                        <  2:846391/139‑1 (MQ=255)

                                                                         tATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTtct                                                      <  2:994673/139‑1 (MQ=255)

                                                                                 ttATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCaa                                              >  2:941333/1‑139 (MQ=255)

                                                                                 ttATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATGTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTGTCACTTCaa                                              >  1:400989/1‑139 (MQ=255)

                                                                                  tATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAAc                                             >  2:42194/1‑139 (MQ=255)

                                                                                            aCCTTCGACGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTg                                   >  1:454757/1‑139 (MQ=255)

                                                                                                    cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  2:20639/139‑1 (MQ=255)

                                                                                                    cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  2:1107731/139‑1 (MQ=255)

                                                                                                    cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  1:739085/139‑1 (MQ=255)

                                                                                                    cGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGc                           <  1:506850/139‑1 (MQ=255)

                                                                                                        gccgccTGTCAGCAAGAGCTGGAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGc                       <  2:517551/139‑1 (MQ=255)

                                                                                                                             gAGGCCTTCTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCg  >  1:1034519/1‑139 (MQ=255)

TAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGCATTATGAGCCGGACCTTCGGCGAAGCCGCCTGTCAGCAAGAGCTGGAGGCCTTTTTAGCCCACCGTGACCAGATTACCGCACTTGATTTAGCCGGTGATGAACTTGGTTTCCCGGGAAGTCTGTTCCTTTCTCACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCG  >  NC_000913/1702567‑1702830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A