Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
71 |
51.6 |
2219666 |
95.9% |
2128659 |
118.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,708,192 |
T→C |
V425A (GTT→GCT) |
rsxC → |
SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,708,192 | 0 | T | C | 100.0%
| 100.8
/ NA
| 27 | V425A (GTT→GCT) | rsxC | SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/9); total (18/9) |
GCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGG > NC_000913/1708060‑1708320
|
gcgcCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGTAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAGGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGccc < 1:1084072/139‑1 (MQ=255)
gcgcCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGccc < 2:730641/139‑1 (MQ=255)
gcCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGa < 1:263653/139‑1 (MQ=255)
ccTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAg < 2:359728/139‑1 (MQ=255)
cTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGc < 2:756650/139‑1 (MQ=255)
gtgCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCaa > 1:118588/1‑139 (MQ=255)
gCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAg > 2:935294/1‑139 (MQ=255)
gTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCt < 1:935294/139‑1 (MQ=255)
gTTCAGCATAGGTCAGCAACACGATAAAGCTACCACGGATAACGTTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCGCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCg > 2:741789/1‑139 (MQ=255)
gTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTcccc < 1:214989/101‑1 (MQ=255)
gTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTcccc > 2:214989/1‑101 (MQ=255)
gTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCa > 1:377143/1‑139 (MQ=255)
cGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAg > 2:341811/1‑139 (MQ=255)
ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAata > 1:710921/1‑63 (MQ=255)
ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAata < 2:710921/63‑1 (MQ=255)
ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGa > 2:73549/1‑139 (MQ=255)
ccACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGa > 1:248000/1‑139 (MQ=255)
cATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCa > 1:39584/1‑139 (MQ=255)
tAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCaaa > 2:484192/1‑139 (MQ=255)
tGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGt > 2:638280/1‑139 (MQ=255)
tGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGt > 2:803650/1‑139 (MQ=255)
cTGATTGCATTGAATGTGGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGttt > 2:1073289/1‑139 (MQ=255)
tgtgGGGCTTGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAgg < 2:118588/139‑1 (MQ=255)
ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg > 2:576670/1‑139 (MQ=255)
ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg > 2:216874/1‑139 (MQ=255)
ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg > 1:420613/1‑139 (MQ=255)
ttGCGCGTGGGCTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAG‑AAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTgg > 2:923350/1‑139 (MQ=255)
|
GCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTGG > NC_000913/1708060‑1708320
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
