Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	78	65.3	3016648	94.6%	2853749	111.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,230	T→C	V63V (GTA→GTG)	ygcU ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,230	0	T	C	100.0%	225.8 / NA	60	V63V (GTA→GTG)	ygcU	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/29); total (31/29)

TTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGA  >  NC_000913/2899098‑2899362

ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGc                                                                                                                                >  2:29596/1‑139 (MQ=255)

ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGc                                                                                                                                >  2:475501/1‑139 (MQ=255)

ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGc                                                                                                                                >  1:991374/1‑139 (MQ=255)

   attCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGctct                                                                                                                             >  2:1341529/1‑139 (MQ=255)

    ttCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCtg                                                                                                                            <  2:58071/139‑1 (MQ=255)

             gAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGa                                                                                                                   >  2:832570/1‑139 (MQ=255)

             gAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGa                                                                                                                   >  2:672171/1‑139 (MQ=255)

              aGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAg                                                                                                                  >  1:842532/1‑139 (MQ=255)

                   tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTa                                                                                                             >  2:1221351/1‑139 (MQ=255)

                       gCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTGTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGAc                                                                                                         >  1:930142/1‑139 (MQ=255)

                        caccacCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGAcc                                                                                                        >  2:1197481/1‑139 (MQ=255)

                             ccGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGc                                                                                                   >  2:568732/1‑139 (MQ=255)

                                aGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCgg                                                                                                >  2:486470/1‑139 (MQ=255)

                                        acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        <  1:1423085/139‑1 (MQ=255)

                                        acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        <  1:672171/139‑1 (MQ=255)

                                         caacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCaa                                                                                       <  1:832570/139‑1 (MQ=255)

                                            caGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGt                                                                                    >  2:581148/1‑139 (MQ=255)

                                             aGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGta                                                                                   <  1:482567/139‑1 (MQ=255)

                                              gTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtat                                                                                  <  2:249913/139‑1 (MQ=255)

                                               tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 >  2:777982/1‑139 (MQ=255)

                                               tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 <  2:703729/139‑1 (MQ=255)

                                               tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 <  1:1230716/139‑1 (MQ=255)

                                               tttCCAGCCCACCTGCGGTGGCGGAAGCACCTGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTACTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata                                                                                 >  2:121977/1‑139 (MQ=255)

                                                ttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATaa                                                                                <  1:581148/139‑1 (MQ=255)

                                                   cAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATg                                                                             <  2:553690/139‑1 (MQ=255)

                                                         aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        >  2:374558/1‑122 (MQ=255)

                                                         aCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCa                                                                                        <  1:374558/122‑1 (MQ=255)

                                                                    cGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGaaa                                                            >  1:583380/1‑139 (MQ=255)

                                                                          cACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTg                                                                                                                                 <  1:632879/64‑1 (MQ=255)

                                                                          cACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTg                                                                                                                                 >  2:632879/1‑64 (MQ=255)

                                                                             cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                   <  1:683342/139‑1 (MQ=255)

                                                                             cGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGaaa                                                   >  1:113549/1‑139 (MQ=255)

                                                                                  cgcgGCACACCGTTAATTTTGTGCGCATCCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                              >  1:399105/1‑139 (MQ=255)

                                                                                          acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGc                                                                                         <  2:1280563/88‑1 (MQ=255)

                                                                                          acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGc                                                                                         >  1:1280563/1‑88 (MQ=255)

                                                                                          acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATa                                         <  2:97099/136‑1 (MQ=255)

                                                                                          acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATa                                         >  1:97099/1‑136 (MQ=255)

                                                                                            cGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGtt                                    >  2:65199/1‑139 (MQ=255)

                                                                                             gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:777982/139‑1 (MQ=255)

                                                                                             gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:1221351/139‑1 (MQ=255)

                                                                                             gTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  2:583380/139‑1 (MQ=255)

                                                                                               tAATTTTGTGCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttttt                                 <  1:568732/139‑1 (MQ=255)

                                                                                                      gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAAt                                                                                             <  1:716410/72‑1 (MQ=255)

                                                                                                      gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAAt                                                                                             >  2:716410/1‑72 (MQ=255)

                                                                                                      gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGGTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAAt                          >  2:927378/1‑139 (MQ=255)

                                                                                                      gtgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCAGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAAt                          >  1:588770/1‑139 (MQ=255)

                                                                                                       tgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTTTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAATTACGAAAACGGTCAATACTGttt                                   >  2:13196/1‑129 (MQ=255)

                                                                                                       tgCGCATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:13196/129‑1 (MQ=255)

                                                                                                           cATTCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGt                     <  1:121977/139‑1 (MQ=255)

                                                                                                              tCATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTc                  >  1:469215/1‑139 (MQ=255)

                                                                                                               cATAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCa                 <  1:29596/139‑1 (MQ=255)

                                                                                                                aTAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCAt                <  1:1197481/139‑1 (MQ=255)

                                                                                                                 tAAAATTCAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATGCGGAAATTTCCGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATc               <  2:930142/139‑1 (MQ=255)

                                                                                                                        cAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   <  1:312404/112‑1 (MQ=255)

                                                                                                                        cAGCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                   >  2:312404/1‑112 (MQ=255)

                                                                                                                          gCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATaa                                                                                >  2:1200935/1‑65 (MQ=255)

                                                                                                                          gCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATaa                                                                                <  1:1200935/65‑1 (MQ=255)

                                                                                                                          gCACACGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAc      <  1:475501/139‑1 (MQ=255)

                                                                                                                              acGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGa  <  1:1341529/139‑1 (MQ=255)

                                                                                                                              acGGGACACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGa  >  2:1104132/1‑139 (MQ=255)

TTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGA  >  NC_000913/2899098‑2899362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A