Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	71	51.6	2219666	95.9%	2128659	118.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,675,350	A→G	G337G (GGT→GGC)	pntB ←	pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,675,350	0	A	G	100.0%	198.5 / NA	53	G337G (GGT→GGC)	pntB	pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/25); total (28/25)

GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCG  >  NC_000913/1675216‑1675485

gtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCAc                                                                                                                                     >  1:274792/1‑139 (MQ=255)

gtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCAc                                                                                                                                     >  2:790981/1‑139 (MQ=255)

   tcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGCTACCGAAACGCACATTAATGCCACGAg                                                                                                                                  <  1:662987/139‑1 (MQ=255)

   tcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                                  <  2:767912/139‑1 (MQ=255)

     aGCAACGTCATCATTGATCTCGTCCATTTCCAGCACGATTTCATACGGTACTCTTGCTTCAGCCAGCAATACGTTCTTACGTCCAGGCAAACGCCCCTCGACCGGCTGGATATCGAAACGCACATTAATGCCACGAgcg                                                                                                                                <  1:157894/139‑1 (MQ=255)

       cAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAgcgcg                                                                                                                              >  2:633931/1‑139 (MQ=255)

             catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAttt                                                                                                                        >  1:864926/1‑139 (MQ=255)

              atcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTtc                                                                                                                       <  1:790981/139‑1 (MQ=255)

               tcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCGACGAGCGCGCAATTtct                                                                                                                      >  2:684438/1‑139 (MQ=255)

                      tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAgaatt                                                                                                               >  2:148913/1‑135 (MQ=255)

                      tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAAt                                                                                                               >  1:1108790/1‑139 (MQ=255)

                      tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAAt                                                                                                               >  1:966978/1‑139 (MQ=255)

                          gTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCa                                                                                                           >  2:680467/1‑139 (MQ=255)

                           tCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                          <  1:977569/139‑1 (MQ=255)

                             cATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCg                                                                                                        <  1:789420/139‑1 (MQ=255)

                                ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                                     <  2:482049/139‑1 (MQ=255)

                                         gATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCCGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                                  >  1:483046/1‑101 (MQ=255)

                                         gATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCCGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAg                                                                                                                                  <  2:483046/101‑1 (MQ=255)

                                          aTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcg                                                                                           >  1:410669/1‑139 (MQ=255)

                                           tGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcgc                                                                                          >  1:637376/1‑139 (MQ=255)

                                                     tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCTGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  1:455158/1‑139 (MQ=255)

                                                     tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAt                                                                                                                          <  2:1073914/97‑1 (MQ=255)

                                                     tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAAt                                                                                                                          >  1:1073914/1‑97 (MQ=255)

                                                     tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  1:169084/1‑139 (MQ=255)

                                                     tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  2:1006965/1‑139 (MQ=255)

                                                     tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                                >  2:767693/1‑139 (MQ=255)

                                                           tGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCTGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                          <  2:455158/139‑1 (MQ=255)

                                                           tGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                          <  2:864926/139‑1 (MQ=255)

                                                               tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                      >  2:12950/1‑139 (MQ=255)

                                                                   ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                                  <  2:76463/139‑1 (MQ=255)

                                                                     agcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                <  2:894297/139‑1 (MQ=255)

                                                                       cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              <  2:94169/139‑1 (MQ=255)

                                                                               tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAt                                                      <  2:996845/139‑1 (MQ=255)

                                                                                          ggCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                           <  2:274792/139‑1 (MQ=255)

                                                                                          ggCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                           <  1:858453/139‑1 (MQ=255)

                                                                                           gCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                          >  2:609560/1‑139 (MQ=255)

                                                                                           gCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                          >  2:618813/1‑139 (MQ=255)

                                                                                               aCGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                                      >  2:833926/1‑139 (MQ=255)

                                                                                                cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              <  2:492452/114‑1 (MQ=255)

                                                                                                cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              >  1:492452/1‑114 (MQ=255)

                                                                                                cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGCTCACTGAATGGGAGtttt                                     >  1:118022/1‑139 (MQ=255)

                                                                                                cGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                                     >  1:30726/1‑139 (MQ=255)

                                                                                                       cgACCGGGTGGATACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                              <  1:833926/139‑1 (MQ=255)

                                                                                                                  aTACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              >  1:531045/1‑96 (MQ=255)

                                                                                                                  aTACCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                              <  2:531045/96‑1 (MQ=255)

                                                                                                                    aCCGAAACGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTctct                 <  2:1106133/139‑1 (MQ=255)

                                                                                                                        aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                >  1:929892/1‑88 (MQ=255)

                                                                                                                        aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                >  1:825713/1‑88 (MQ=255)

                                                                                                                        aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                <  2:825713/88‑1 (MQ=255)

                                                                                                                        aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCg                                                                <  2:929892/88‑1 (MQ=255)

                                                                                                                        aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGt                                                            <  2:139204/92‑1 (MQ=255)

                                                                                                                        aaaCGCACATTAATGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGt                                                            >  1:139204/1‑92 (MQ=255)

                                                                                                                                   aaTGCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTcg  <  2:783109/139‑1 (MQ=255)

GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTCG  >  NC_000913/1675216‑1675485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A