Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
71 |
51.6 |
2219666 |
95.9% |
2128659 |
118.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,505,331 |
T→C |
A230A (GCA→GCG) |
yhfS ← |
FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,505,331 | 0 | T | C | 100.0%
| 134.7
/ NA
| 36 | A230A (GCA→GCG) | yhfS | FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/22); total (14/22) |
CAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGC > NC_000913/3505201‑3505462
|
cAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTg < 1:292276/139‑1 (MQ=255)
tCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGAt < 2:246456/139‑1 (MQ=255)
gcgcTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGc < 2:1015584/139‑1 (MQ=255)
cgcTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCt < 1:1040115/139‑1 (MQ=255)
cgcTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCt > 1:275156/1‑139 (MQ=255)
cTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAc < 2:348259/139‑1 (MQ=255)
cTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAc < 1:479812/139‑1 (MQ=255)
gcaccgcaccACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAg > 2:947409/1‑139 (MQ=255)
gcaccgcaccACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAg > 2:413723/1‑139 (MQ=255)
gcaccgcaccACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACAGCGTGCATCACTGGCGCAAACATCAGACCACGCAATACTTCCAGCGCATGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAg > 1:547947/1‑139 (MQ=255)
atcgaaccgccGTTACGCAAAGCCAGCAACCATTAAGATACCCCTGTTCGTTCCGCGTGCATCACTGGCGTAAACCCCTGCCCTCGAAATCATTCCAGCGCCTGCGCGCACTTGATCTGGCTACCGCCGGAGTAAAGCg < 1:1067348/134‑1 (MQ=255)
caccacCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGgc < 1:529372/139‑1 (MQ=255)
ccGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGaa > 1:103118/1‑139 (MQ=255)
gTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATa < 1:259866/139‑1 (MQ=255)
ttAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc < 1:413723/139‑1 (MQ=255)
ttAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc > 2:195702/1‑139 (MQ=255)
aaGCAAAGCCAGCAACGGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACgg < 2:455034/139‑1 (MQ=255)
aaCCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAg < 1:311510/139‑1 (MQ=255)
ctgcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAccgc < 2:67852/84‑1 (MQ=255)
ctgcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTAccgc > 1:67852/1‑84 (MQ=255)
gcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGaa > 1:502336/1‑103 (MQ=255)
gcctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGaa < 2:502336/103‑1 (MQ=255)
ctgcACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCa < 1:306033/139‑1 (MQ=255)
gcgTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCct < 1:201559/139‑1 (MQ=255)
cgTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCctc > 2:810489/1‑139 (MQ=255)
gCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTgg < 1:611998/139‑1 (MQ=255)
cTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCaaa < 1:1023119/139‑1 (MQ=255)
ccAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGAc < 1:253258/82‑1 (MQ=255)
ccAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGAc > 2:253258/1‑82 (MQ=255)
cAGCGCCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTc > 2:765245/1‑139 (MQ=255)
gcgcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATa < 2:615299/53‑1 (MQ=255)
gcgcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATa > 1:615299/1‑53 (MQ=255)
gcgcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTcgc > 1:321979/1‑139 (MQ=255)
gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc < 1:656708/52‑1 (MQ=255)
gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATAc > 2:656708/1‑52 (MQ=255)
gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGAt < 2:463332/59‑1 (MQ=255)
gcCTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGAt > 1:463332/1‑59 (MQ=255)
cTGCGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGAt < 2:781589/57‑1 (MQ=255)
|
CAATCACCGCGCTTTTCACTTCCGGCACCGCACCACCGTTAAGCAAAGCCAGCAACCGTTCAGATACCCCTGCCTGCACCGCGTGCATCACTGGCGCAAACACCAGACCACGCAATACTTCCAGCGCCTGTGCGCCCTGGATCTGGCTACCGCCGGAGTAAAGCGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGC > NC_000913/3505201‑3505462
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
