Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
78 |
48.0 |
2243616 |
94.8% |
2126947 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,846,908 |
A→G |
V18A (GTC→GCC) |
topB ← |
DNA topoisomerase III |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,846,908 | 0 | A | G | 100.0%
| 81.4
/ NA
| 24 | V18A (GTC→GCC) | topB | DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/9); total (15/9) |
CGCATAGCGGCTGTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGACATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAGTTCGCCAATT > NC_000913/1846784‑1847019
|
cgcATAGCGGCTGTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTAGGCAGGGCATCAGCAATGgcg > 2:657979/1‑139 (MQ=255)
atgCGGCTGACGTAGGCTTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCATGTCACCACCTGACAATTACCGCACCCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGGAATGgcgcgcg < 1:658537/137‑1 (MQ=255)
gTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCaa > 1:179702/1‑122 (MQ=255)
gTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCaa < 2:179702/122‑1 (MQ=255)
gTAGGCGTCTGGCTGCGCCTGCTCAAGCTGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGt > 2:694582/1‑139 (MQ=255)
gCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGttttt < 1:657979/139‑1 (MQ=255)
cGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTc < 1:289005/139‑1 (MQ=255)
cTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGc > 1:1077870/1‑139 (MQ=255)
ccTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAaacaa < 1:295135/139‑1 (MQ=255)
tGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTaa > 1:1050523/1‑139 (MQ=255)
aTACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAAtc > 1:794582/1‑139 (MQ=255)
aGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCa > 1:69527/1‑139 (MQ=255)
caccacCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGc > 2:839624/1‑139 (MQ=255)
accaccTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCa > 1:205139/1‑139 (MQ=255)
caccTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACg > 1:1116416/1‑139 (MQ=255)
tACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCcgcgcg < 1:694582/139‑1 (MQ=255)
cACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCAt < 1:375670/117‑1 (MQ=255)
cACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCAt > 2:375670/1‑117 (MQ=255)
gATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAg > 2:355028/1‑139 (MQ=255)
tCGCCTTTCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAGTTCGCCAAtt > 1:442027/1‑139 (MQ=255)
tCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAAc < 2:739541/92‑1 (MQ=255)
tCCGGTGCGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGACAATAAACAACCGCATTGAATTAACGAATCTCAAc > 1:739541/1‑92 (MQ=255)
cGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCa < 2:248588/65‑1 (MQ=255)
cGGTTTGGGCAGGGCATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCa > 1:248588/1‑65 (MQ=255)
|
CGCATAGCGGCTGTCGTAGGCGTCTGGCTGCGCCTGCTCAAGCAGGTGACCGATACACCAGGTCACCACCTGACCATTACCGCACTCGATAAAGCCATCGCCTTTCCGGTGCGGTTTGGGCAGGACATCAGCAATGGCGCGCGCCAGACTCGGTTTTTCGGCAATAAACAACCGCATTGAATTAACGAATCTCAACCATGGCACGACCGCCGCGCGCTGGCACCAGTTCGCCAATT > NC_000913/1846784‑1847019
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
