Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I2 R1 78 48.0 2243616 94.8% 2126947 114.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,538,491 C→T G92D (GGC→GAC)  nanM ← N‑acetylneuraminic acid mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,4910CT98.5% 232.3 / NA 67G92D (GGC→GAC) nanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (34/32);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGCCGTAC  >  NC_000913/4538356‑4538620
                                                                                                                                       |                                                                                                                                 
ttGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                                <  1:256570/139‑1 (MQ=255)
ttGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                                <  1:326607/139‑1 (MQ=255)
  gtgtACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCTATGTCGCCa                                                                                                                              <  1:1048486/139‑1 (MQ=255)
  gtgtACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                              <  2:358899/139‑1 (MQ=255)
    gtACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                            <  1:904427/139‑1 (MQ=255)
    gtACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                            <  2:773777/139‑1 (MQ=255)
             tCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAg                                                                                                                   >  2:930999/1‑139 (MQ=255)
             tCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAg                                                                                                                   >  2:661279/1‑139 (MQ=255)
             tCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAg                                                                                                                   >  1:307157/1‑139 (MQ=255)
               acaTGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                            <  2:26723/128‑1 (MQ=255)
               acaTGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                            >  1:26723/1‑128 (MQ=255)
                    gCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                            <  2:687440/139‑1 (MQ=255)
                      ccGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATc                                                                                                          >  1:413928/1‑139 (MQ=255)
                         ccATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAAt                                                                                                       <  1:394142/139‑1 (MQ=255)
                              cccATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATg                                                                                                  >  2:418289/1‑139 (MQ=255)
                                   cGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                            <  2:567017/124‑1 (MQ=255)
                                   cGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                            >  1:567017/1‑124 (MQ=255)
                                    ggCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAgg                                                                                            <  2:512488/139‑1 (MQ=255)
                                      cgcgTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCATTGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGtt                                                                                          >  1:625213/1‑139 (MQ=255)
                                        cgTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGc                                                                                        >  1:867334/1‑139 (MQ=255)
                                                 tCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATctct                                                                               <  2:865147/139‑1 (MQ=255)
                                                            cAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAg                                                                    >  1:461926/1‑139 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTaa                                                       >  2:282748/1‑139 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTaa                                                       >  2:524558/1‑139 (MQ=255)
                                                                         tGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTaa                                                       >  2:1048934/1‑139 (MQ=255)
                                                                          ggggTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                                <  2:155393/65‑1 (MQ=255)
                                                                          ggggTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                                >  1:155393/1‑65 (MQ=255)
                                                                          ggggTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGTCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAAc                                                      >  2:735774/1‑139 (MQ=255)
                                                                          ggggTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAAc                                                      >  2:178915/1‑139 (MQ=255)
                                                                            ggTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                                <  2:57141/63‑1 (MQ=255)
                                                                            ggTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                                >  1:57141/1‑63 (MQ=255)
                                                                                gTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTc                                                <  2:413928/139‑1 (MQ=255)
                                                                                gTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTc                                                <  1:943266/139‑1 (MQ=255)
                                                                                      gtgtACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCAtttt                                          >  1:1114930/1‑139 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                              <  2:163693/50‑1 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                              >  1:163693/1‑50 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                            >  1:726527/1‑68 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCa                                                                                                            <  2:726527/68‑1 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATc                                     >  1:607962/1‑139 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATc                                     >  1:303878/1‑139 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATc                                     >  1:793192/1‑139 (MQ=255)
                                                                                            gTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCt                                    <  1:745036/139‑1 (MQ=255)
                                                                                                ttAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTgg                                <  1:856864/139‑1 (MQ=255)
                                                                                                ttAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTgg                                <  1:417882/139‑1 (MQ=255)
                                                                                                ttAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTgg                                >  2:399304/1‑139 (MQ=255)
                                                                                                  aaaTACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGcc                              <  2:867334/139‑1 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCGGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                        >  1:1112007/1‑139 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                        >  2:519612/1‑139 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                        >  2:376203/1‑139 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                        >  2:1070270/1‑139 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                        >  2:136114/1‑139 (MQ=255)
                                                                                                         tGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCGGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                       <  2:1112007/139‑1 (MQ=255)
                                                                                                         tGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                       <  2:997768/139‑1 (MQ=255)
                                                                                                         tGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                       <  2:1114930/139‑1 (MQ=255)
                                                                                                         tGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                       <  1:376203/139‑1 (MQ=255)
                                                                                                           aGTCAAGCTCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                       >  1:814651/1‑137 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACTTACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                       <  2:814651/137‑1 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAgg                                                                                            <  1:1035731/68‑1 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAgg                                                                                            >  2:1035731/1‑68 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAAt                                                               <  2:45904/97‑1 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAAt                                                               >  1:45904/1‑97 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATcc                     <  1:343467/139‑1 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATcc                     >  2:786868/1‑139 (MQ=255)
                                                                                                             tCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTGCATTTTTTATCTTTGGCCTGTGTATCCAg                   >  2:758607/1‑139 (MQ=255)
                                                                                                              cAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGc                  <  1:786868/139‑1 (MQ=255)
                                                                                                               aaGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCAttttt                                         >  2:509615/1‑115 (MQ=255)
                                                                                                               aaGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCAttttt                                         <  1:509615/115‑1 (MQ=255)
                                                                                                                aGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCtt                <  2:303878/139‑1 (MQ=255)
                                                                                                                      cGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGcc                              <  1:930405/119‑1 (MQ=255)
                                                                                                                      cGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGcc                              >  2:930405/1‑119 (MQ=255)
                                                                                                                           tttttGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCtg                            <  2:1063962/116‑1 (MQ=255)
                                                                                                                           tttttGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCtg                            >  1:1063962/1‑116 (MQ=255)
                                                                                                                              ttGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGCCGTAc  <  1:418289/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
TTGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGCCGTAC  >  NC_000913/4538356‑4538620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A