Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
57 |
49.8 |
2216334 |
94.8% |
2101084 |
113.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,735,897 |
T→C |
Y174H (TAT→CAT) |
sodB → |
superoxide dismutase, Fe |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,735,897 | 0 | T | C | 100.0%
| 148.8
/ NA
| 40 | Y174H (TAT→CAT) | sodB | superoxide dismutase, Fe |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/23); total (17/23) |
AAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTA > NC_000913/1735761‑1736027
|
aaaCAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCAt < 1:133908/139‑1 (MQ=255)
cGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCACTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAg > 1:863548/1‑139 (MQ=255)
gATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGc < 2:1009034/139‑1 (MQ=255)
aaaCTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCt < 1:363250/139‑1 (MQ=255)
tttCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTggg > 2:902231/1‑129 (MQ=255)
tttCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTggg < 1:902231/129‑1 (MQ=255)
ttCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAAc < 1:924034/139‑1 (MQ=255)
ggTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGa > 1:230472/1‑139 (MQ=255)
cTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg < 2:393861/139‑1 (MQ=255)
cTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg < 1:62416/139‑1 (MQ=255)
ttgctaccGGTCCGACCCCGGTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTACTGGCCATCTGGAGCATTTCTGGGCTGTGGTGAACTGGAAATTCGTAACGAAAACTCTCGc > 2:1049451/6‑139 (MQ=255)
tGACCCCCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATTCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc < 1:688707/139‑1 (MQ=255)
tGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc < 2:508439/139‑1 (MQ=255)
accaccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg < 1:771759/139‑1 (MQ=255)
accaccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg < 1:501832/139‑1 (MQ=255)
cGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGc < 2:899901/139‑1 (MQ=255)
ctgctgACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc < 2:50621/119‑1 (MQ=255)
ctgctgACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc > 1:50621/1‑119 (MQ=255)
cGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg < 1:879933/110‑1 (MQ=255)
cGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCg > 2:879933/1‑110 (MQ=255)
tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg < 1:906043/91‑1 (MQ=255)
tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg > 2:906043/1‑91 (MQ=255)
tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTAt < 1:965790/139‑1 (MQ=255)
tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTAt < 2:83755/139‑1 (MQ=255)
ttaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCa > 1:632817/1‑139 (MQ=255)
tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGa < 1:795575/54‑1 (MQ=255)
tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGa > 2:795575/1‑54 (MQ=255)
tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCt > 2:493215/1‑139 (MQ=255)
gACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTa > 2:179066/1‑138 (MQ=255)
gACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTa < 1:179066/138‑1 (MQ=255)
gACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTAc > 2:20565/1‑139 (MQ=255)
aCTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTAca > 1:329162/1‑138 (MQ=255)
cGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg > 1:716844/1‑76 (MQ=255)
cGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg < 2:716844/76‑1 (MQ=255)
cAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGatca < 2:230472/139‑1 (MQ=255)
tGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc > 2:599554/1‑69 (MQ=255)
tGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGc < 1:599554/69‑1 (MQ=255)
gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAAtctc < 2:674163/62‑1 (MQ=255)
gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAAtctc > 1:674163/1‑62 (MQ=255)
gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTa > 2:546416/1‑139 (MQ=255)
|
AAACAGCGATGGCAAACTGGCTATCGTTTCAACCTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTA > NC_000913/1735761‑1736027
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
