Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
82 |
28.6 |
1225488 |
97.4% |
1193625 |
116.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,968,408 |
C→T |
V32M (GTG→ATG) |
cheB ← |
fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,968,408 | 0 | C | T | 100.0%
| 75.8
/ NA
| 23 | V32M (GTG→ATG) | cheB | fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/14); total (9/14) |
GCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACA > NC_000913/1968279‑1968526
|
gCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCAt > 2:420464/1‑139 (MQ=255)
ttCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTtg > 1:13853/1‑139 (MQ=255)
cGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTtgat < 1:130279/139‑1 (MQ=255)
cGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTtgat < 1:284781/139‑1 (MQ=255)
aaaTCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGAttt < 2:43828/139‑1 (MQ=255)
aaTCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTc < 2:89941/139‑1 (MQ=255)
tCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAatcat < 1:268180/139‑1 (MQ=255)
cAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCg < 2:13853/139‑1 (MQ=255)
aGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGa > 2:384515/1‑139 (MQ=255)
tCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATc > 1:116076/1‑110 (MQ=255)
tCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATc < 2:116076/110‑1 (MQ=255)
aaTTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGAttt > 1:584171/1‑82 (MQ=255)
aaTTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGAttt < 2:584171/82‑1 (MQ=255)
aaTCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGtt < 2:561659/139‑1 (MQ=255)
aGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATcc < 2:495015/139‑1 (MQ=255)
tCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGtt < 2:74019/132‑1 (MQ=255)
tCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGtt > 1:74019/1‑132 (MQ=255)
gcgcgACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCcttactt < 1:420464/139‑1 (MQ=255)
cgcgACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCcttactta > 1:564887/1‑139 (MQ=255)
gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGa > 2:320116/1‑49 (MQ=255)
gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGa < 1:320116/49‑1 (MQ=255)
gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATaca < 1:214787/126‑1 (MQ=255)
gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATaca > 2:214787/1‑126 (MQ=255)
|
GCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACA > NC_000913/1968279‑1968526
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
