Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	82	28.6	1225488	97.4%	1193625	116.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,971,962	G→A	R244C (CGC→TGC)	tar ←	methyl‑accepting chemotaxis protein II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,971,962	0	G	A	100.0%	127.5 / NA	38	R244C (CGC→TGC)	tar	methyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/17); total (21/17)

CGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATA  >  NC_000913/1971843‑1972087

cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         >  1:309352/1‑126 (MQ=255)

cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         <  2:309352/126‑1 (MQ=255)

cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTa                                                                                                            >  2:314866/1‑139 (MQ=255)

                ccGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTaccac                                                                                            >  1:210185/1‑139 (MQ=255)

                      tAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGa                                                                                      <  2:535082/139‑1 (MQ=255)

                                   aaCCTTCGCGGACATGAGAGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAAtgtg                                                                         >  1:276765/1‑139 (MQ=255)

                                         cgcgGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGt                                                                                                                   <  1:560418/91‑1 (MQ=255)

                                         cgcgGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGt                                                                                                                   >  2:560418/1‑91 (MQ=255)

                                         cgcgGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCaat                                                                   >  2:417089/1‑139 (MQ=255)

                                                  gAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGc                                                          <  1:578766/139‑1 (MQ=255)

                                                    gTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCa                                                        <  1:417089/139‑1 (MQ=255)

                                                     agACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAg                                                       <  2:276765/138‑1 (MQ=255)

                                                          gtgtCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         <  1:63082/68‑1 (MQ=255)

                                                          gtgtCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         >  2:63082/1‑68 (MQ=255)

                                                             tcagtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTaa                                               >  1:362041/1‑139 (MQ=255)

                                                               agtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTa                                                                                                            >  2:197842/1‑76 (MQ=255)

                                                               agtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTa                                                                                                            <  1:197842/76‑1 (MQ=255)

                                                                     agagCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAg                                                       >  1:287194/1‑123 (MQ=255)

                                                                     agagCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAg                                                       <  2:287194/123‑1 (MQ=255)

                                                                         cGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCa                                                                                                                        >  2:207784/1‑54 (MQ=255)

                                                                         cGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCa                                                                                                                        <  1:207784/54‑1 (MQ=255)

                                                                         cGTTGCATATGAGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGc                                   <  1:390194/139‑1 (MQ=255)

                                                                             gCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAAt                               >  1:166346/1‑139 (MQ=255)

                                                                              cATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGa                                 >  2:50854/1‑136 (MQ=255)

                                                                              cATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGa                                 <  1:50854/136‑1 (MQ=255)

                                                                                      aaaCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAg                                              >  2:502269/1‑115 (MQ=255)

                                                                                      aaaCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAg                                              <  1:502269/115‑1 (MQ=255)

                                                                                      aaaCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCa                      >  2:509170/1‑139 (MQ=255)

                                                                                        aCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGc                             >  1:196025/1‑130 (MQ=255)

                                                                                        aCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGc                             <  2:196025/130‑1 (MQ=255)

                                                                                        aCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACg                    >  1:509684/1‑139 (MQ=255)

                                                                                           ctctGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAAtgt                                                                          >  2:292616/1‑82 (MQ=255)

                                                                                           ctctGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAAtgt                                                                          <  1:292616/82‑1 (MQ=255)

                                                                                                cgcCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACcagc            <  2:527507/139‑1 (MQ=255)

                                                                                                        cGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCaa    >  2:435839/1‑139 (MQ=255)

                                                                                                          cccATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCa     >  2:298631/1‑136 (MQ=255)

                                                                                                          cccATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCa     <  1:298631/136‑1 (MQ=255)

                                                                                                          cccATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATa  >  2:179989/1‑139 (MQ=255)

CGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATA  >  NC_000913/1971843‑1972087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A