Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
73 |
24.7 |
1166784 |
93.9% |
1095610 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,520,008 |
C→T |
L328L (CTG→TTG) |
curA → |
curcumin/dihydrocurcumin reductase, NADPH‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,520,008 | 0 | C | T | 100.0%
| 75.5
/ NA
| 23 | L328L (CTG→TTG) | curA | curcumin/dihydrocurcumin reductase, NADPH‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/12); total (11/12) |
CGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCA > NC_000913/1519878‑1520137
|
cGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAg > 1:215963/1‑139 (MQ=255)
cGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAg > 1:45119/1‑139 (MQ=255)
gCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAGAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAgg > 2:466756/1‑139 (MQ=255)
cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAGAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa < 1:466756/139‑1 (MQ=255)
cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa < 2:45119/139‑1 (MQ=255)
cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa < 2:117657/139‑1 (MQ=255)
cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa < 2:215963/139‑1 (MQ=255)
aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGACAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCaa > 2:378786/1‑139 (MQ=255)
aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCaa > 1:62981/1‑139 (MQ=255)
cGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGa > 1:431949/1‑118 (MQ=255)
cGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGa < 2:431949/118‑1 (MQ=255)
agGGAGATGGGTCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGACTGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCgg < 2:360550/139‑1 (MQ=255)
aTGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGtgatg > 2:41621/1‑139 (MQ=255)
cGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa > 2:542986/1‑59 (MQ=255)
cGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa < 1:542986/59‑1 (MQ=255)
gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa > 2:458368/1‑46 (MQ=255)
gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa < 1:458368/46‑1 (MQ=255)
gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAtt < 2:157174/76‑1 (MQ=255)
gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAtt > 1:157174/1‑76 (MQ=255)
cacaGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGt < 1:167170/65‑1 (MQ=255)
cacaGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGt > 2:167170/1‑65 (MQ=255)
tttATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACca < 1:378786/139‑1 (MQ=255)
tttATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACca < 2:62981/139‑1 (MQ=255)
|
CGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCA > NC_000913/1519878‑1520137
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
