Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
73 |
24.7 |
1166784 |
93.9% |
1095610 |
107.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,806,491 |
G→A |
G41D (GGC→GAC) |
pfkB → |
6‑phosphofructokinase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,806,491 | 0 | G | A | 100.0%
| 59.5
/ NA
| 18 | G41D (GGC→GAC) | pfkB | 6‑phosphofructokinase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/7); total (11/7) |
TGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAG > NC_000913/1806389‑1806619
|
tGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAgg > 1:5631/1‑139 (MQ=255)
gACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGc > 1:368851/1‑139 (MQ=255)
gACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGc > 2:485406/1‑139 (MQ=255)
acacTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCa > 1:482151/1‑139 (MQ=255)
acacTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCa > 2:236607/1‑139 (MQ=255)
cacTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAg < 1:236607/139‑1 (MQ=255)
aacaaTTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGTGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGt > 2:175796/1‑139 (MQ=255)
aacaaTTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGt > 1:276759/1‑139 (MQ=255)
ttACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGgcgc < 2:482151/139‑1 (MQ=255)
aCCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGa < 2:368851/139‑1 (MQ=255)
aCCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTc > 1:559488/1‑139 (MQ=255)
gtgtTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg > 2:489677/1‑139 (MQ=255)
gtgtTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg > 2:397682/1‑139 (MQ=255)
ttCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCg < 2:570106/139‑1 (MQ=255)
gggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAg < 1:489677/139‑1 (MQ=255)
gggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAg < 2:5631/139‑1 (MQ=255)
ggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt < 2:252728/127‑1 (MQ=255)
ggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt > 1:252728/1‑127 (MQ=255)
|
TGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAG > NC_000913/1806389‑1806619
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
