Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	81	28.9	1316934	95.8%	1261622	113.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,108	T→C	*471Q (TAA→CAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,108	0	T	C	100.0%	83.6 / NA	23	*471Q (TAA→CAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/11); total (12/11)

TTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAT  >  NC_000913/1756982‑1757229

ttCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCtttt                                                                                                               >  2:2168/1‑139 (MQ=255)

    aCCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACTTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTACCACCGCAGCTGTTCACGCCCTGCAATATTGCTTTTGTGa                                                                                                           <  1:290420/139‑1 (MQ=255)

       gTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaatt                                                                                                        <  1:78057/139‑1 (MQ=255)

          tGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaattaat                                                                                                     <  1:175806/139‑1 (MQ=255)

                  gAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTatat                                                                                             >  1:417160/1‑139 (MQ=255)

                      tGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGaa                                                                                         <  2:429190/139‑1 (MQ=255)

                             gCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaattaat                                                                                                     <  2:567880/120‑1 (MQ=255)

                             gCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGaattaat                                                                                                     >  1:567880/1‑120 (MQ=255)

                                                            gTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGACTTAATTTGTATATCGAAGCGCCCTGATGGGCGATTTTTTTATTTAATCGATAAcc                                                   >  1:223739/1‑139 (MQ=255)

                                                            gTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAcc                                                   >  2:478756/1‑139 (MQ=255)

                                                                        tCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATaaa                                       >  1:42445/1‑139 (MQ=255)

                                                                                        cGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGAttt                       <  2:42445/139‑1 (MQ=255)

                                                                                         gAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTc                      <  1:478756/139‑1 (MQ=255)

                                                                                         gAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTc                      >  2:33707/1‑139 (MQ=255)

                                                                                             ggCACTACTAACACCGCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAGTTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACta                  <  2:417160/139‑1 (MQ=255)

                                                                                                         actgCATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCACTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACt      <  1:33707/136‑1 (MQ=255)

                                                                                                             cATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAt  >  2:33685/1‑139 (MQ=255)

                                                                                                             cATCTGTTCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAt  >  1:178476/1‑139 (MQ=255)

                                                                                                             cATCTGTTCACGTCCTGCAATATTGCTATTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGAATTTCACTATATAATCTCACTTTAt  >  2:425403/1‑139 (MQ=255)

                                                                                                                   ttCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGa                                                                                          <  1:239050/45‑1 (MQ=255)

                                                                                                                   ttCACGTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGa                                                                                          >  2:239050/1‑45 (MQ=255)

                                                                                                                        gTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcg                                                                          >  1:370542/1‑56 (MQ=255)

                                                                                                                        gTCCTGCAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcg                                                                          <  2:370542/56‑1 (MQ=255)

TTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAAAAATCAAATCGGATTTCACTATATAATCTCACTTTAT  >  NC_000913/1756982‑1757229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A