Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
81 |
28.9 |
1316934 |
95.8% |
1261622 |
113.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,597,778 |
A→G |
intergenic (‑160/+53) |
purC ← / ← bamC |
phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,597,778 | 0 | A | G | 100.0%
| 87.9
/ NA
| 24 | intergenic (‑160/+53) | purC/bamC | phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/13); total (11/13) |
ACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATG > NC_000913/2597644‑2597912
|
aCGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGtt > 2:74634/1‑139 (MQ=255)
aaaaTCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGtat < 2:431350/139‑1 (MQ=255)
ttGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGtt < 1:472900/124‑1 (MQ=255)
ttGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGtt > 2:472900/1‑124 (MQ=255)
cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgatg > 1:568/1‑139 (MQ=255)
cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgatg > 2:345011/1‑139 (MQ=255)
gTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc < 2:181421/139‑1 (MQ=255)
aaCGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGga < 2:524937/112‑1 (MQ=255)
aaCGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGga > 1:524937/1‑112 (MQ=255)
aCGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCt < 1:534171/139‑1 (MQ=255)
ttATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg < 2:89558/139‑1 (MQ=255)
ttATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg < 2:568/139‑1 (MQ=255)
aTCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCa > 2:560787/1‑139 (MQ=255)
aaCTACGTCCGACAAACAAATTTCATGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTg < 2:368431/139‑1 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat > 1:644421/1‑94 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat < 2:644421/94‑1 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg < 2:517209/126‑1 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg > 1:517209/1‑126 (MQ=255)
tCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACa > 2:259534/1‑139 (MQ=255)
acaaaTTTCGTGCGAATTACCGCTAAAATCACGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAg > 1:256057/1‑139 (MQ=255)
tttCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGcgt < 1:74634/139‑1 (MQ=255)
gCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCt < 1:345011/139‑1 (MQ=255)
gCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGa < 2:462010/139‑1 (MQ=255)
gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATg > 1:184157/1‑139 (MQ=255)
|
ACGTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATG > NC_000913/2597644‑2597912
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
