Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
78 |
65.3 |
3016648 |
94.6% |
2853749 |
111.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,597,778 |
A→G |
intergenic (‑160/+53) |
purC ← / ← bamC |
phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,597,778 | 0 | A | G | 100.0%
| 244.0
/ NA
| 63 | intergenic (‑160/+53) | purC/bamC | phosphoribosylaminoimidazole‑succinocarboxamide synthetase/BamABCDE complex OM biogenesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/36); total (27/36) |
GTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATG > NC_000913/2597646‑2597912
|
gTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg < 2:1191117/139‑1 (MQ=255)
gTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg < 1:790708/139‑1 (MQ=255)
tttGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGttt < 1:1020374/126‑1 (MQ=255)
tttGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGttt > 2:1020374/1‑126 (MQ=255)
gTCTTGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTACGTATCATATCAGaaaaa < 1:993309/139‑1 (MQ=255)
tCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGTGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGaaaaaa > 1:1030368/1‑139 (MQ=255)
tCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCCGCAGACGCACGCGTTTGCGTATCATATCAGaaaaaa > 2:1045108/1‑139 (MQ=255)
gtgtGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGc < 1:1227908/139‑1 (MQ=255)
gtgtGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGc < 1:601238/139‑1 (MQ=255)
tgCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCgg > 2:952053/1‑139 (MQ=255)
ccGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGta < 2:586397/115‑1 (MQ=255)
ccGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGta > 1:586397/1‑115 (MQ=255)
ccGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat < 1:613535/139‑1 (MQ=255)
cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGga > 2:1334489/1‑137 (MQ=255)
cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGga < 1:1334489/137‑1 (MQ=255)
cGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGAGTTTTCCTGCAGACGCACGCGTTTGCGTATAATATCAGAAAAAAGGGCCGgatg > 2:993309/1‑139 (MQ=255)
tGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGcc < 1:952053/139‑1 (MQ=255)
gTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc < 2:1411140/139‑1 (MQ=255)
gTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc < 2:418859/139‑1 (MQ=255)
gATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTa > 2:1330982/1‑139 (MQ=255)
tGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGtat > 1:414304/1‑99 (MQ=255)
tGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGtat < 2:414304/99‑1 (MQ=255)
aaCGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGc > 1:681688/1‑139 (MQ=255)
aCGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGTGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCt < 2:1030368/139‑1 (MQ=255)
ttATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg > 2:672537/1‑139 (MQ=255)
cGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGa > 1:1412139/1‑139 (MQ=255)
gtacGACAAACAAATTTCATGCGAATTACAGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg < 1:957879/63‑1 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg > 2:957879/1‑66 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc > 1:179693/1‑105 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGccc < 2:179693/105‑1 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGAc > 2:47203/1‑139 (MQ=255)
gTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGAc > 2:289870/1‑139 (MQ=255)
tCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACa > 2:176732/1‑139 (MQ=255)
acaaaTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg > 1:326179/1‑57 (MQ=255)
acaaaTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTg < 2:326179/57‑1 (MQ=255)
caaaTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAgc < 2:33248/139‑1 (MQ=255)
aTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGcgcg < 1:1330982/139‑1 (MQ=255)
aTTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGcgcg < 1:176732/139‑1 (MQ=255)
tttCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGcgt < 1:672537/139‑1 (MQ=255)
tttCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGcgt < 1:47203/139‑1 (MQ=255)
cGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATAACAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTA‑CAGCGCGTCGtt < 1:1007283/139‑1 (MQ=255)
tGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat < 1:1151370/75‑1 (MQ=255)
tGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGgat > 2:1151370/1‑75 (MQ=255)
tGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACTGCTACCAGCGCGTCGTTc > 1:1013536/1‑139 (MQ=255)
aTTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct < 2:1412139/139‑1 (MQ=255)
aTTACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct < 2:1215877/139‑1 (MQ=255)
tACCGCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGActct < 1:1486446/139‑1 (MQ=255)
gCTAAAATCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGt < 2:603671/139‑1 (MQ=255)
aaaTCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAg > 2:195402/1‑68 (MQ=255)
aaaTCGCGTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAg < 1:195402/68‑1 (MQ=255)
cgcgTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTAt > 2:179395/1‑139 (MQ=255)
gcgTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCt > 2:212605/1‑120 (MQ=255)
gcgTTTTCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCt < 1:212605/120‑1 (MQ=255)
ttCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGcc > 1:1397485/1‑87 (MQ=255)
ttCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGcc < 2:1397485/87‑1 (MQ=255)
tCCTGCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCttt > 2:402724/1‑139 (MQ=255)
gCAGACGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGa < 1:1045108/139‑1 (MQ=255)
cGCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCGGAGTATGACCTTTCGGATCGAt < 1:289870/139‑1 (MQ=255)
gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg > 1:512651/1‑71 (MQ=255)
gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACg < 2:512651/71‑1 (MQ=255)
gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct > 2:711602/1‑108 (MQ=255)
gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGAct < 1:711602/108‑1 (MQ=255)
gCACGCGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATg < 1:402724/139‑1 (MQ=255)
|
GTTTTAAAATCTTTTGCTGTCTGGTGTGCCGGATGTTTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACGTCCGACAAACAAATTTCGTGCGAATTACCGCTAAAATCGCGTTTTCCTGCAGACGCACACGTTTGCGTATCATATCAGAAAAAAGGGCCGGATGATTCCAGCCCTGTATTTTTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATG > NC_000913/2597646‑2597912
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
