Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
81 |
28.9 |
1316934 |
95.8% |
1261622 |
113.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,189,993 |
A→G |
E38G (GAG→GGG) |
yqiH → |
putative periplasmic pilin chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,189,993 | 0 | A | G | 100.0%
| 103.2
/ NA
| 28 | E38G (GAG→GGG) | yqiH | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/13); total (15/13) |
AAAATTAATTCAGGATGAAACTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAA > NC_000913/3189858‑3190118
|
aaaaTTAATTCAGGATGAAACTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTc > 1:611304/1‑139 (MQ=255)
tAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa < 2:611304/139‑1 (MQ=255)
cagcagGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCt > 1:265750/1‑139 (MQ=255)
agGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc < 2:502886/139‑1 (MQ=255)
agGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc < 1:66952/139‑1 (MQ=255)
ctctCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa < 2:340032/139‑1 (MQ=255)
tcCTGTATGAGTAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag > 1:621630/1‑139 (MQ=255)
tcCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag > 2:284193/1‑139 (MQ=255)
tcCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag < 2:265750/139‑1 (MQ=255)
gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa > 1:332962/1‑91 (MQ=255)
gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa < 2:332962/91‑1 (MQ=255)
agcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAg > 1:523090/1‑139 (MQ=255)
caTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGc > 2:277679/1‑139 (MQ=255)
aTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa < 1:108209/95‑1 (MQ=255)
aTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa > 2:108209/1‑95 (MQ=255)
catcatCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCaaa < 2:435252/101‑1 (MQ=255)
catcatCCCGGATCGTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATAAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCaaa > 1:435252/1‑101 (MQ=255)
gTACCCGTATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGt < 1:284193/139‑1 (MQ=255)
tATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc < 1:527374/76‑1 (MQ=255)
tATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc > 2:527374/1‑76 (MQ=255)
tATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCa > 1:604655/1‑139 (MQ=255)
tATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCa > 2:54665/1‑139 (MQ=255)
tATCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCa < 2:549864/139‑1 (MQ=255)
aTCATAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCaa > 2:535832/1‑139 (MQ=255)
aTAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAGAAACCTGCCCTATCTGGCCCAAACa < 2:479595/78‑1 (MQ=255)
aTAATGAATGGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTTGCCCAAACa > 1:479595/1‑78 (MQ=255)
aatgaatgGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa < 1:564919/45‑1 (MQ=255)
aatgaatgGGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa > 2:564919/1‑45 (MQ=255)
|
AAAATTAATTCAGGATGAAACTAATGCGCTACTTAAATACAAAGAATATCATTGCAGCAGGAGTATTACTCTCCTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAAGATACGAAAGGCAATAAATCGCGTGACTTTATTGTCACTGTTCCTCCAA > NC_000913/3189858‑3190118
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
