Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
81 |
28.9 |
1316934 |
95.8% |
1261622 |
113.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,607,418 |
C→T |
A323V (GCC→GTC) |
zntA → |
zinc, cobalt and lead efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,607,418 | 0 | C | T | 100.0%
| 96.5
/ NA
| 29 | A323V (GCC→GTC) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/13); total (16/13) |
TTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACG > NC_000913/3607286‑3607548
|
ttGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGCCGTTGGAAGTGCTGTCAGAACCGGGAGTCAgcgc > 1:362523/1‑139 (MQ=255)
ttGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCg < 1:178145/139‑1 (MQ=255)
aGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGa > 2:364428/1‑139 (MQ=255)
cgcCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGaaa < 1:350261/139‑1 (MQ=255)
gACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATc > 2:493755/1‑139 (MQ=255)
tGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCAt < 1:94173/97‑1 (MQ=255)
tGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCAt > 2:94173/1‑97 (MQ=255)
cgcgcAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTc > 2:571403/1‑139 (MQ=255)
cAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg < 1:277936/132‑1 (MQ=255)
cAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgcg > 2:277936/1‑132 (MQ=255)
cAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCAt > 1:281376/1‑139 (MQ=255)
ggCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGc < 2:20564/139‑1 (MQ=255)
gATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGt < 1:571403/139‑1 (MQ=255)
tAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATAc > 1:571970/1‑139 (MQ=255)
tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa < 1:468877/111‑1 (MQ=255)
tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa > 2:468877/1‑111 (MQ=255)
tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAg > 2:489608/1‑120 (MQ=255)
tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAg < 1:489608/120‑1 (MQ=255)
tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGccc > 2:435335/1‑136 (MQ=255)
tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCcgcg > 2:539863/1‑139 (MQ=255)
tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAACAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGccc < 1:435335/136‑1 (MQ=255)
cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCgaag > 1:424608/1‑70 (MQ=255)
cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCgaag < 2:424608/70‑1 (MQ=255)
cGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGt > 1:523735/1‑116 (MQ=255)
cGTTGGAAGTGCTGTCAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGt < 2:523735/116‑1 (MQ=255)
cAGAACCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGt > 2:283397/1‑139 (MQ=255)
aCCGGGAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACg < 1:364428/139‑1 (MQ=255)
gAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa > 1:557761/1‑79 (MQ=255)
gAGTCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGa < 2:557761/79‑1 (MQ=255)
|
TTGCCAGTTTTGATGAAAGCGCCCTGACCGGCGAATCCATTCCGGTGGAGCGCGCAACGGGCGATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTCAGCCGTATCTATACGCCCGCGATTATGGCCGTCGCTCTGCTGGTGACG > NC_000913/3607286‑3607548
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
