Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I51 R1	223	69.9	1534784	95.1%	1459579	214.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	921,236	A→C	K297T (AAA→ACA)	macB →	macrolide ABC transporter peremase/ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	921,236	0	A	C	97.6%	146.7 / NA	42	K297T (AAA→ACA)	macB	macrolide ABC transporter peremase/ATPase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (21/20); total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGTGCGCAATCC‑TCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGC  >  NC_000913/921007‑921468

cttGTGCTCAATCC‑CCCCGCCATTGAAAAAGTGAATGTTGCTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCcacacaaa                                                                                                                                                                                                                                     <  1:95426/234‑1 (MQ=255)

     ggcgaaTCCTTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTg                                                                                                                                                                                                                                <  1:183029/232‑1 (MQ=255)

              ccgccGCCATTGAAAAAGGGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGATGGCGGCAGTTGGTCAGGGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGat                                                                                                                                                                                                                       <  2:500598/233‑1 (MQ=255)

                   gCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCAGGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCg                                                                                                                                                                                                                  <  1:221529/236‑1 (MQ=255)

                          aaaagttaaaTGTTACTTGCTGGACGAAACCTGTGTCCAACGCGGTGACTGGCTGCAGGCAGTTTGTGAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                                                                                                                                                           <  2:406437/228‑1 (MQ=255)

                          aaaagGGGAATGTTACTGGCAGGACGGAACCTGTTGTCAACACGGTGTCGGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                                                                                                                                                           <  2:166022/236‑1 (MQ=255)

                             atgTGAATGTTCAGGGCGGGACGGAACCGGTTGTCAACCCGGTGTCTGGTTGGCGTCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATTTTCGTTCTATTGGt                                                                                                                                                                                                        <  2:612250/234‑1 (MQ=255)

                                    tGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACg                                                                                                                                                                                                     <  2:193955/232‑1 (MQ=255)

                                    tGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGaa                                                                                                                                                                                                   <  1:54490/234‑1 (MQ=255)

                                    tGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGaa                                                                                                                                                                                                   <  1:441636/234‑1 (MQ=255)

                                          gggCGGGACGGAACCTGTTGTCATCGCGGTGTCTGGCTGGCGGCAGTTGGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTAtt                                                                                                                                                                                            <  2:408964/234‑1 (MQ=255)

                                                          gttgtCAACCCGGTGTCTGGCTGGCGGCAGTTTGTCAGGGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGaa                                                                                                                                                                           <  2:670993/236‑1 (MQ=255)

                                                                     ggTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGGACTTTTCTGACCATTCTGGGGGTTATTTTCGGTATTGCGTCGGGGGGTTTCATTTTCGTGGTGGGTGACGCCGCCACACACATGGGGCGGGCGGGTATTCGGTCTTTTTGGTCGCATACCAATTAAGTCTATCCCGGGGAACAattt                                                                                                                                                                    >  2:249014/1‑232 (MQ=255)

                                                                                 gcggcAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATTCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCg                                                                                                                                                     <  1:249014/235‑1 (MQ=255)

                                                                                                                                              aaTAAAGTGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTTTTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGc                                                                                       <  2:187281/236‑1 (MQ=255)

                                                                                                                                                        gTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGcc                                                                                >  1:643144/1‑233 (MQ=255)

                                                                                                                                                         tACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGA‑TTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGAAGACTTAATCGCCATCCAAAAACAACCGTGGGTGGCCTCAGcc                                                                                >  1:423223/1‑231 (MQ=255)

                                                                                                                                                                cTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGt                                                                     <  1:368161/236‑1 (MQ=255)

                                                                                                                                                                     cATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGc                                                                >  1:10857/1‑236 (MQ=255)

                                                                                                                                                                             ggATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGATGACTTAATCGCCATCCAAAAAAAACCGTGGGTCGCCTCAGCCAACCCTGCCGTCTCGCAAAAcct                                                         >  2:369431/1‑235 (MQ=255)

                                                                                                                                                                              gATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCTCACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAACAAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgc                                                       >  2:696638/1‑236 (MQ=255)

                                                                                                                                                                                    tagcGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcctg                                                  <  1:369431/232‑1 (MQ=255)

                                                                                                                                                                                      tCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGt                                               <  1:601204/236‑1 (MQ=255)

                                                                                                                                                                                        ggTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTTGCGATGACGATCCGCAATATCAGCAAGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTTAGCCCCAACCTCCGTCTCGCCAAACCTGCGCCTGCGtt                                              >  2:250092/1‑235 (MQ=255)

                                                                                                                                                                                         gTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTAc                                                                                                                                                                                                      <  1:122297/82‑1 (MQ=255)

                                                                                                                                                                                         gTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTAc                                                                                                                                                                                                      >  2:122297/1‑82 (MQ=255)

                                                                                                                                                                                         gTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGAAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTGGCCTCAGCCAACCCTGCCGTTCCGCCAAACATGCGCCTACGGtat                                            >  2:187612/1‑236 (MQ=255)

                                                                                                                                                                                           aTTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATaa                                          >  1:501900/1‑236 (MQ=255)

                                                                                                                                                                                                   ggtggtTTCAATTGTCGGGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAatgttg                                  <  2:643144/236‑1 (MQ=255)

                                                                                                                                                                                                    gtggtTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAACTACGCCTGCGTTATAACAatggtga                                 >  2:238360/1‑236 (MQ=255)

                                                                                                                                                                                                         ttCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGGTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTTCCGCAAAACCCGAGCCTGCGTTTTAGCAatgttgatgtt                             >  2:321806/1‑235 (MQ=255)

                                                                                                                                                                                                               tGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCg                                                                                                                                                     >  1:433509/1‑109 (MQ=255)

                                                                                                                                                                                                               tGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCg                                                                                                                                                     <  2:433509/109‑1 (MQ=255)

                                                                                                                                                                                                                      gtgggtgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCa                >  1:729477/1‑235 (MQ=255)

                                                                                                                                                                                                                      gtgggtgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCa                >  2:509062/1‑235 (MQ=255)

                                                                                                                                                                                                                           tgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGc                                                                                       >  1:531516/1‑159 (MQ=255)

                                                                                                                                                                                                                           tgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGc                                                                                       <  2:531516/159‑1 (MQ=255)

                                                                                                                                                                                                                           tgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCg          <  1:509062/236‑1 (MQ=255)

                                                                                                                                                                                                                           tgACGCCGCCACACAAATGGCGCTGGCGGATATTCGCTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCCCCATCCCACAACAACCCTGGGCCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCCCCTGCGTTATAACAATCTTGATCTTGCTGCCAGGGCCaaagcgc          >  2:213937/1‑231 (MQ=255)

                                                                                                                                                                                                                            gACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGCCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAAAAAAGTTGATGTTGCTGCCCGTGCCAATGGCGt         >  2:417694/1‑236 (MQ=255)

                                                                                                                                                                                                                              cgccgcCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGGCTCGCCAAAACGGCGCCTGCGTTTTACCAATGTTTATGTTGCTGCCAGTTCCAATGGCCTGa       >  2:681365/1‑236 (MQ=255)

                                                                                                                                                                                                                                 cgccACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTCTAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAgcg    >  1:414549/1‑236 (MQ=255)

                                                                                                                                                                                                                                   ccACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGAAGACTTAATCGCCCTCCAACAACAACCGTGGGTCGCCTCCGCCCCACCTACCGTCTCGCAAAACCTGCGCCTGCGTTTTAACACATTTGAGTTTTCTGCCAGTGTCAATGTCGttgagtgc  >  2:75070/1‑229 (MQ=255)

ATTGTGCGCAATCC‑TCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGC  >  NC_000913/921007‑921468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A