Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 921,236 A→C K297T (AAA→ACA)  macB → macrolide ABC transporter peremase/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913921,2360AC98.3% 395.4 / ‑3.6 118K297T (AAA→ACA) macBmacrolide ABC transporter peremase/ATPase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (64/51);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 121 total aligned reads displayed.

TGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCA  >  NC_000913/921140‑921332
                                                                                                |                                                                                                
tGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCcacaca                                                                                               <  1:1399533/100‑1 (MQ=255)
 ggCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGGTTCCATTGGCGTGGTGGGTGACGCCGCcacacaa                                                                                              >  2:1459076/1‑100 (MQ=255)
   cAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAAt                                                                                            >  1:1448448/1‑100 (MQ=255)
    aGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATg                                                                                           <  1:3368560/100‑1 (MQ=255)
     gCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATgg                                                                                          <  1:3575456/100‑1 (MQ=255)
     gCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATgg                                                                                          >  2:473599/1‑100 (MQ=255)
      cGAATCAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGt                                                                                         <  1:3868210/100‑1 (MQ=255)
       gAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTg                                                                                        <  1:2030809/100‑1 (MQ=255)
        aaTAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGc                                                                                       <  1:3171155/100‑1 (MQ=255)
        aaTAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTCGGTTGCGCCGCCACACAAATGGTGc                                                                                       >  2:3789109/1‑100 (MQ=255)
         aTAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCt                                                                                      >  2:2696775/1‑100 (MQ=255)
               ttcGTACTCTACTGACTATGCTGGGGATTATTCTCGGTAGTGCGTCGGTGGTTTCCATTGTCGTTGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGa                                                                                <  2:2771417/98‑1 (MQ=255)
               tGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGa                                                                                >  2:2290481/1‑100 (MQ=255)
                gCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGat                                                                               <  2:3061595/100‑1 (MQ=255)
                   tACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATAtt                                                                            <  2:1602737/100‑1 (MQ=255)
                   tACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATAtt                                                                            >  1:2298936/1‑100 (MQ=255)
                    gcTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTc                                                                           <  2:1145119/99‑1 (MQ=255)
                     cTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCg                                                                          <  2:1396822/100‑1 (MQ=255)
                       ttACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGtt                                                                        >  2:1957791/1‑100 (MQ=255)
                         aCTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCt                                                                      >  1:494123/1‑100 (MQ=255)
                         aCTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCt                                                                      >  1:3280637/1‑100 (MQ=255)
                         aCTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCt                                                                      <  1:3208606/100‑1 (MQ=255)
                          cTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTa                                                                     <  1:1222921/100‑1 (MQ=255)
                            gACCCTGCCGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCCTTGTCGTGGTGGGTGACGCCGCCACACAAATGTTGCTGGCGGATATTCGCGCTAtt                                                                   >  2:373069/1‑100 (MQ=255)
                            gACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                   >  2:117727/1‑100 (MQ=255)
                            gACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                   <  2:1675733/100‑1 (MQ=255)
                            gACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                   >  2:2183622/1‑100 (MQ=255)
                            gACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                   >  1:2949878/1‑100 (MQ=255)
                            gACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                   >  1:2941892/1‑100 (MQ=255)
                            gACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                   >  2:3744604/1‑100 (MQ=255)
                              ccATGCTGGGGATTATTATCGTTATTGCGTCAGTGGTTTCCCTTGTCGTGGTGGGTGACGCCGCCACACAGATGGTGCTGGCGGATATTCGTTCTATTgg                                                                 >  2:3807204/1‑100 (MQ=255)
                              ccATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTgg                                                                 <  2:1626981/100‑1 (MQ=255)
                              ccATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTgg                                                                 <  1:3763244/100‑1 (MQ=255)
                                 tGCTGGGGATTATTATCGGTATTGCGTCGGGGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAAGGGTGCTTGCGGGTAGTCGTTCTATTGGTAc                                                              >  2:182622/1‑100 (MQ=255)
                                  gCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACg                                                             >  2:2878205/1‑100 (MQ=255)
                                  gCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACg                                                             >  2:2878568/1‑100 (MQ=255)
                                    tGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGaa                                                           >  2:1346627/1‑100 (MQ=255)
                                     ggggATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAAt                                                          <  1:2035875/100‑1 (MQ=255)
                                      gggATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATa                                                         >  1:2699927/1‑100 (MQ=255)
                                        gATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCCTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACt                                                       <  2:3634577/100‑1 (MQ=255)
                                         attattATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTa                                                      >  1:767204/1‑100 (MQ=255)
                                          ttattaTCGGTATCGCGTCGGTGGTTTCCATTGTCGGGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTACCAATTGAACGAATatgcg                                                     <  2:2654354/100‑5 (MQ=255)
                                            attatCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTg                                                   <  2:1428792/100‑1 (MQ=255)
                                            attatCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTg                                                   >  1:3417514/1‑100 (MQ=255)
                                            attatCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTg                                                   >  1:2765584/1‑100 (MQ=255)
                                            attatCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTg                                                   <  1:771098/100‑1 (MQ=255)
                                             ttatCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGa                                                  >  1:1706960/1‑100 (MQ=255)
                                             ttatCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGa                                                  >  1:827922/1‑100 (MQ=255)
                                              tatCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGAt                                                 <  1:414402/100‑1 (MQ=255)
                                               atCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGTCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATg                                                <  1:3563352/100‑1 (MQ=255)
                                               atCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATg                                                >  1:1562816/1‑100 (MQ=255)
                                               atCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATg                                                <  1:3660383/100‑1 (MQ=255)
                                               atCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATg                                                >  1:2384895/1‑100 (MQ=255)
                                                tCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGt                                               >  1:3039527/1‑100 (MQ=255)
                                                 cGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTc                                              <  2:3795239/100‑1 (MQ=255)
                                                 cGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTc                                              <  2:1897331/100‑1 (MQ=255)
                                                 cGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTc                                              <  1:2582480/100‑1 (MQ=255)
                                                    tATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTAt                                           >  1:1076192/1‑100 (MQ=255)
                                                     aTTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATc                                          >  1:74299/1‑100 (MQ=255)
                                                       tGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATccc                                        >  1:1493812/1‑100 (MQ=255)
                                                        gCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCg                                       <  2:2348236/100‑1 (MQ=255)
                                                        gCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCg                                       >  1:3058984/1‑100 (MQ=255)
                                                          gTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCggg                                     <  1:3518385/100‑1 (MQ=255)
                                                           tCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGa                                    >  1:767447/1‑100 (MQ=255)
                                                           tCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGa                                    <  2:2263787/100‑1 (MQ=255)
                                                             ggtggtTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGaaa                                  >  2:470800/1‑100 (MQ=255)
                                                             ggtggtTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGaaa                                  >  2:470951/1‑100 (MQ=255)
                                                                ggtTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGAt                               >  2:1190517/1‑100 (MQ=255)
                                                                ggtTTCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGAt                               >  1:2643282/1‑100 (MQ=255)
                                                                  tttCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGAttt                             <  2:3596528/100‑1 (MQ=255)
                                                                  tttCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGAttt                             >  1:1183592/1‑100 (MQ=255)
                                                                  tttCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGAttt                             >  1:953691/1‑100 (MQ=255)
                                                                  tttCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGGTCTATTGGTACGAATCCTCTTGATGTCTATCCCGGGAAAGAttt                             >  1:2689614/1‑100 (MQ=255)
                                                                   ttCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGAtttt                            >  2:3861493/1‑100 (MQ=255)
                                                                    tCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTg                           >  1:1634720/1‑100 (MQ=255)
                                                                    tCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTg                           >  1:3107089/1‑100 (MQ=255)
                                                                    tCCATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTg                           <  2:355257/100‑1 (MQ=255)
                                                                     ccATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTgg                          >  2:2051595/1‑100 (MQ=255)
                                                                     ccATTGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTgg                          <  1:3340011/100‑1 (MQ=255)
                                                                         tGTCGTTGTGGGTGAGGCCGCCAGACAAATGGGGCGGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGAt                      <  2:483086/100‑1 (MQ=255)
                                                                         tGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGAt                      <  2:2257177/100‑1 (MQ=255)
                                                                         tGTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGAt                      >  1:2846531/1‑100 (MQ=255)
                                                                          gTCGTGGTGGGTGACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATg                     >  1:4079749/1‑100 (MQ=255)
                                                                                gtgggtgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATc               >  2:2995827/1‑100 (MQ=255)
                                                                                 tgggtgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATcc              >  1:3511799/1‑100 (MQ=255)
                                                                                  gggtgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCg             >  2:3845597/1‑100 (MQ=255)
                                                                                    gtgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCa           <  2:1582370/100‑1 (MQ=255)
                                                                                    gtgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCa           >  1:2853358/1‑100 (MQ=255)
                                                                                     tgACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCaa          <  1:3902503/100‑1 (MQ=255)
                                                                                      gACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAat         <  1:2827443/100‑1 (MQ=255)
                                                                                      gACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAat         <  1:2325998/100‑1 (MQ=255)
                                                                                      gACGCCGCCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAat         >  1:1237486/1‑100 (MQ=255)
                                                                                        cgccgcCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAatat       >  1:244919/1‑100 (MQ=255)
                                                                                        cgccgcCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAatat       <  1:398618/100‑1 (MQ=255)
                                                                                        cgccgcCACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAatat       >  2:2087040/1‑100 (MQ=255)
                                                                                          ccgccACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATca     <  2:2670506/100‑1 (MQ=255)
                                                                                          ccgccACACAAACGGTGCGAGCGGATATTCGTCCTATTTGTACTAAAACTATTTAACACTATCCTAGAAAAGATTTTGGCGATGCCGACCCGCAatggca     >  2:1550472/1‑96 (MQ=255)
                                                                                            gccACACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATcagc   >  2:3105497/1‑100 (MQ=255)
                                                                                             ccACACAAATGGTGCTGGCGGATATCCGTTATATTGGTACGACTACTACTGCTGTCTCTCCCGGGCAAGATTTTGGCGATGACGATCCGCAATATcagca  <  2:114249/100‑1 (MQ=255)
                                                                                             ccAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATcagca  <  1:3829783/100‑1 (MQ=255)
                                                                                                |                                                                                                
TGGCAGCGAATAAAATGCGTACTTTACTGACCATGCTGGGGATTATTATCGGTATTGCGTCGGTGGTTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCA  >  NC_000913/921140‑921332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A