Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,078,882 T→G M1M (ATG→CTG) † putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,078,8820TG100.0% 220.9 / NA 59M1M (ATG→CTG) †putAfused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/33);  total (26/33)

GGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCATGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTGAGAG  >  NC_000913/1078653‑1079108
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  
ggCTGGTGTGGTTCCTCTGCCGGAGGCGTGGCATCATCGCTCTCATTGGCCGCGCCAGAAAGCAGGGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCAt                                                                                                                                                                                                                               <  2:30965/235‑1 (MQ=255)
 gCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCAtt                                                                                                                                                                                                                              >  2:61942/1‑235 (MQ=255)
     gtgtGGTTCCGCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTc                                                                                                                                                                                                                          <  1:261280/235‑1 (MQ=255)
                ctGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGa                                                                                                                                                                                                              <  1:215303/236‑1 (MQ=255)
                 tGCCGGAGTTGGTACTTAAGCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGaa                                                                                                                                                                                                             <  2:217729/236‑1 (MQ=255)
                     ggAGTCGTGACTGCAGCGCTCCAATTGCCCGGCGCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGt                                                                                                                                                                                                         <  2:245865/236‑1 (MQ=255)
                       aGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTg                                                                                                                                                                                                        <  1:74088/235‑1 (MQ=255)
                           ggTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTCCTCCTGTTGTTCAGAAAGGTGCAAc                                                                                                                                                                                                    >  2:352702/1‑235 (MQ=255)
                                   aTCGCTTTCAGTGGCCGCGCCAGAAAGCAGCGGAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGATGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTa                                                                                                                                                                                           <  2:452027/236‑1 (MQ=255)
                                       ctctCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGt                                                                                                                                                                                       >  1:273409/1‑236 (MQ=255)
                                        tctgATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAAATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTg                                                                                                                                                                                      <  1:547573/236‑1 (MQ=255)
                                           cATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGaaa                                                                                                                                                                                   <  1:61942/236‑1 (MQ=255)
                                                  cgcgCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTAc                                                                                                                                                                                                                            >  1:123838/1‑188 (MQ=255)
                                                  cgcgCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTAc                                                                                                                                                                                                                            <  2:123838/188‑1 (MQ=255)
                                                      ccAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTAATCGTGACAAATCCatgat                                                                                                                                                                         >  2:385795/1‑235 (MQ=255)
                                                              agacgagtgTAGCTCGGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGc                                                                                                                                                                    <  2:555666/224‑1 (MQ=255)
                                                                 cgcAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTtg                                                                                                                                                             >  1:690767/1‑236 (MQ=255)
                                                                  gaaGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACt                                                                                                                                                                <  1:748092/230‑1 (MQ=255)
                                                                     ggTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTtgtg                                                                                                                                                           <  1:470996/234‑1 (MQ=255)
                                                                          cTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAAc                                                                                                                                                       <  1:385795/233‑1 (MQ=255)
                                                                           tCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATg                                                                                                                                                   >  1:378554/1‑236 (MQ=255)
                                                                              ggCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGt                                                                                                                                                  <  1:67896/235‑1 (MQ=255)
                                                                                cAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGa                                                                                                                                                                                                              >  2:86567/1‑172 (MQ=255)
                                                                                cAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGa                                                                                                                                                                                                              <  1:86567/172‑1 (MQ=255)
                                                                                 atagTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAAt                                                                                                                                             <  2:378554/234‑1 (MQ=255)
                                                                                               ttCCAGTTGTTCGAGATAAGAAATAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGc                                                                                                                               <  2:671993/236‑1 (MQ=255)
                                                                                               ttCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGc                                                                                                                               <  1:23275/236‑1 (MQ=255)
                                                                                                          cGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTaaaaa                                                                                                                    <  2:690767/236‑1 (MQ=255)
                                                                                                                       aaTCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATg                                                                                                         <  1:550999/234‑1 (MQ=255)
                                                                                                                            ccTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACt                                                                                                                                                                                                   <  2:22508/139‑1 (MQ=255)
                                                                                                                            ccTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACt                                                                                                                                                                                                   >  1:22508/1‑139 (MQ=255)
                                                                                                                             cTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATaaa                                                                                                 >  1:99530/1‑236 (MQ=255)
                                                                                                                                cTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAg                                                                                               <  2:99530/235‑1 (MQ=255)
                                                                                                                                   aattaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  2:273409/236‑1 (MQ=255)
                                                                                                                                   aagtaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  1:730850/233‑1 (MQ=255)
                                                                                                                                    attaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  1:733167/235‑1 (MQ=255)
                                                                                                                                    attaaCCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAAt                                                                                           <  1:462371/235‑1 (MQ=255)
                                                                                                                                             tgtggtgtgCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACa                                                                                 >  1:278859/1‑236 (MQ=255)
                                                                                                                                             tgtggtgtgCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACa                                                                                 >  1:483213/1‑236 (MQ=255)
                                                                                                                                              gtggtgtaCTATCGTTCCGTGGCACGGCAGACGTAATCCGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGAc                                                                                  <  2:752395/234‑1 (MQ=255)
                                                                                                                                                         tcgatACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTa                                                                                                                                                >  1:655755/1‑161 (MQ=255)
                                                                                                                                                         tcgatACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTa                                                                                                                                                <  2:655755/161‑1 (MQ=255)
                                                                                                                                                              aCGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAAc                                                                >  1:203349/1‑236 (MQ=255)
                                                                                                                                                                   tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAAc                                                                                                                                                       >  2:690527/1‑144 (MQ=255)
                                                                                                                                                                   tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAAc                                                                                                                                                       <  1:690527/144‑1 (MQ=255)
                                                                                                                                                                   tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACt                                                               <  2:5513/232‑1 (MQ=255)
                                                                                                                                                                   tCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACt                                                               >  1:5513/1‑232 (MQ=255)
                                                                                                                                                                                      cTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAaat                                                                                                                   <  2:548999/161‑1 (MQ=255)
                                                                                                                                                                                      cTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAaat                                                                                                                   >  1:548999/1‑161 (MQ=255)
                                                                                                                                                                                        cACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTaa                                      >  1:297437/1‑236 (MQ=255)
                                                                                                                                                                                          cgcgtcgcgtCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACa                                    >  1:507205/1‑236 (MQ=255)
                                                                                                                                                                                                                  ccccATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCaaa             <  2:170709/236‑1 (MQ=255)
                                                                                                                                                                                                                     cATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCaaaaaa          >  1:547501/1‑236 (MQ=255)
                                                                                                                                                                                                                     cATGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGAAAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTACCATTTTTGAAAGGTGCAACCGCaacaaa          >  2:64184/1‑236 (MQ=255)
                                                                                                                                                                                                                      aTGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAAt         >  1:460998/1‑236 (MQ=255)
                                                                                                                                                                                                                      aTGGTGGTGGTTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAAAATTTTTGAAAGGTGCAACCGGCAAAAAt         >  2:568356/1‑236 (MQ=255)
                                                                                                                                                                                                                            gtggtTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTgaga   >  2:516579/1‑236 (MQ=255)
                                                                                                                                                                                                                            gtggtTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTgaga   >  2:524632/1‑236 (MQ=255)
                                                                                                                                                                                                                             tggtTCCCAGGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTgagag  >  2:526597/1‑236 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  
GGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAA‑CCCCCATGGTGGTGGTTCCCATGCCATTACTCCTGTTGTTCAGAAAGGTGCAACTTAACGTTATCGTGAAATATCCATGATGTTGCAACTTTGTGCAACCATGTTAAATGTGACATGCGTAGCAAGCTTAAAAATGAATGAAATGTTAATAAAAGAAATCGATATGACAGGGATTAAAAAAATAACTCAGACTTTTTCTCTGCGGCAGTTAACATTTTTGAAAGGTGCAACCGCAAAAAATGTGAGAG  >  NC_000913/1078653‑1079108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A