Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F9 I0 R1	89	94.4	2603848	74.4%	1937262	227.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,438	G→A	19.4%	intergenic (‑182/‑25)	ttdR ← / → ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,438	0	G	A	19.4%	90.2 / 4.6	50	intergenic (‑182/‑25)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (6/32); new base A (2/7); total (10/40)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAC  >  NC_000913/3206208‑3206657

tttCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑Ac                                                                                                                                                                                                                        <  1:68724/239‑1 (MQ=255)

ttgctccctgTAACTGCAAGTCTTTGGCTAAAGGCCAGGTATTCAGCATCGTTTATTATCCGCCGTTGTGATAAGGGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGGAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑Ac                                                                                                                                                                                                                        <  2:141636/230‑1 (MQ=255)

   tctcctgtcacTGCAAGTTTTTGGCATAAGGGCAGGTTTTCAGGAGTGTTAATTATCCGCAGTTTTGATAAGGCGAGTTTAGTTCGCAAAACATTGCCAGTCCCTGCTCTGGCGCAATATTCATTCTTATTTGCGCGTTGACTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACaaa                                                                                                                                                                                                                     <  2:65876/229‑1 (MQ=255)

     aCCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTAATTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAAATTTTA‑CACTCAaaa‑aaaaaaa                                                                                                                                                                                                                   >  1:1112574/1‑239 (MQ=255)

          tACCTGCAAGTCTTTGGCTAAAGTCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGAGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCCATATTCATTCTTATTTTCGCGTTGATTCCCGAACTTTTAATTCAATATTACCGGCGCCAAAAATTTTAAAACACC‑ATTTTATGGTGATATTTTGTGAGGG‑TGCATTTTCC‑CA‑AAAAAA‑ACaaaaaacatg                                                                                                                                                                                                              >  2:559609/1‑234 (MQ=255)

          tACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTT‑CAGCACAAT‑Aaaaaaaaacaa                                                                                                                                                                                                              >  1:789733/1‑234 (MQ=255)

           aCCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAg                                                                                                                                                                                                             <  1:907732/239‑1 (MQ=255)

                     cTTTGGCTAAAGGCCAGCTATTCAGCATCGGTAATTATCCGCAGTTGTTATAAGCGCAGTGTATTTCGTAAAACAGTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑atg                                                                                                                                                                                                    <  1:112481/237‑1 (MQ=255)

                        tGGTTAAAGGCCAGCTTTTCAGCATCGTTAATTATCCCCATGTGTGAAAAGCGCAGTGTATTTCGGAAAACATTGCCATTCCCTGCTCTTGCGCAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAg                                                                                                                                                                                               <  2:525281/239‑1 (MQ=255)

                        tGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAg                                                                                                                                                                                               <  1:1085298/239‑1 (MQ=255)

                            tAAAGGCCAGCTATTCAGCCTCGTTAATTATCCGGAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGa                                                                                                                                                                                            <  1:777638/238‑1 (MQ=255)

                            tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGa                                                                                                                                                                                            <  1:752292/238‑1 (MQ=255)

                            tAAAGGACTACTATTCAGCATCGTTAATTATTCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACTGTGCCATTCCCTGCTCTGGCGCAATATTCAGTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTG‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGa                                                                                                                                                                                            <  2:764225/238‑1 (MQ=255)

                                                     aaTTTTCCGCAGTTGTGATAAGCGCAGTTTTTTTCTGAAAACATTGCCCTTCCCTGCTCTGGCTCAATATTCATTCTTATGTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGTGAATa                                                                                                                                                                 <  2:33148/239‑1 (MQ=255)

                                                      ttttttcccatttGTGAATAGCGCTTGGTATTTCGCAAAACATTGCCACTCCCTGCTCTGGGGCAAGATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGTGAATaa                                                                                                                                                                <  2:725053/228‑1 (MQ=255)

                                                      aTTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGTGAATaa                                                                                                                                                                <  1:450741/239‑1 (MQ=255)

                                                           ccGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGGAATTTTT‑CAGACCAAA‑AAAAAAAATAGAGTTGCC‑ATGATTGGCGGAAGGAATAAGACAAAAGACAGTGAaaaattga                                                                                                                                                            >  1:765516/1‑235 (MQ=255)

                                                                         aGCGCAGTGTATTTCGCAAAACAGTGCTATTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGCt                                                                                                                                            <  1:638722/239‑1 (MQ=255)

                                                                         aGCGAAGTGTATTTCGCAAACCATTCGCATTCCCTGCTTGGGCGCAATATTCATTCTTATTTTCGGGTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGGGGCGG‑TAAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑AGGATGAGCGAAAGTAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGCt                                                                                                                                            <  1:559609/239‑1 (MQ=255)

                                                                              attgtTTTTCGCAAAACATTGCCATTCCCTGCTCTTGCGCAATATTCATTCTTATTTTGGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CATCAGCCAGTGAATAAGT‑TGACAGAGATTGTCGCTAACt                                                                                                                                        <  1:579773/236‑1 (MQ=255)

                                                                                                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTT‑TCAACAACA‑ACAAAAACATGAATTGCC‑ATGGTGAGCGAAAGGAAAAAA‑AAAAAGACAGAGAAAAAAA‑TGACAACAAATTTCGCTAACATTACccccccaatatctac                                                                                                                     >  2:1024431/1‑227 (MQ=255)

                                                                                                         cttgttctGGCTAAATTTTCATTCTTATTTCCGCGTTGATTCCCGACCGTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGGGG‑TAAATTTTCC‑CAAACAAAT‑ACAAAACCTGGAGTTGCA‑ATGATGAGCGAAATTAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGcc                                                                                                            <  1:85648/234‑1 (MQ=255)

                                                                                                             ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTT‑CCAACAAAA‑AAAAAAACAGGAGTTGCC‑ATGATGAGCGGAAGAAATAAA‑CAACAAGCAGGGAATAAGA‑TGACAAAGGTTGTTGCTAACTTTACCGCCATGATTTTTTCCCCAACGccccat                                                                                                        >  1:1125536/1‑235 (MQ=255)

                                                                                                             ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑GGAATTTTTT‑CAGACAAAA‑AAAAAAAAAGGAGGTGCC‑CTGTTGAGGGGAAGAAATAAA‑CAACAAGAAGTGAATAAAT‑TGTCAGAGGTTGTTGCGAACTTTACTGCCCTGAGTTCTACCCCAAAACCtgat                                                                                                        >  1:890630/1‑239 (MQ=255)

                                                                                                                    gcAATATTCATTCTTATTTGCGAGTTGATCCACGAACTGTTAATTACATAGTCACGGTGGAAAAAAATAAAAAACCGC‑ATTTT‑TGGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGAAAAAT‑AAAAAAACGGGAGTTGCC‑ATGATGAGCGAAAGAAAGAAG‑CAACAGGCAGTGAATAAGT‑GGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                  <  1:98986/238‑1 (MQ=255)

                                                                                                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑GTAATTTTTT‑CAGACAAAT‑AAAAAAAATAGAGTTGCC‑ATGATTAGCGGAAGTAATAAA‑AAAAACGCAATGTAAAAAA‑TTAGAGAGATTTTTGCCAAATTTTCCGCCACGAGTTTTATCCGCATACCCCATTAACAGGGGGGTAAACTaaaa                                                                                   >  1:276076/1‑239 (MQ=255)

                                                                                                                                                    caaatttttAATTACATATTCAGGGGGGAAAAAAATAAAAAACCAA‑ATTTT‑TAGGGGTATTTTGGGGCGG‑TGATTTTTAC‑CAGACAAAT‑AAAAAAACGGGAGTTGCC‑ATGATGAGCAAAAGTAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                 <  1:975898/232‑1 (MQ=255)

                                                                                                                                                             aaTTAAATATCCACGGTGGAAAAAAATATAAACCCAC‑ATTTT‑GAGGGGGAGTTGGTGGCGG‑TGATTTTTTC‑CAGACAAAA‑AAAAAATCTGGAGTGACC‑ATAAGGAGCAAAAGTAATAAG‑CAACAGGAAGTAAATAAGT‑GGACAGAGTTTGTCGCTATTTTTACCGCCATGATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                        <  1:1063473/239‑1 (MQ=255)

                                                                                                                                                              attaattatTCCCGGTGGCAAAAAATATAAAACCAC‑TTTTT‑TAGGGGTAGTTTGGGGCGG‑TGAATTTTTC‑CAAAAAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGAGAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa                                                       <  1:966527/233‑1 (MQ=255)

                                                                                                                                                                tACATATTCGCGGTGAAAAAAAATAAAAAACCAA‑TTTTT‑TAGTGGTAGTTTGGGGCGG‑TGAATTTTTC‑CACAAAAAT‑CAAAAAACTGGATTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGTTAAAAAGT‑TGAAAGAGATTTTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATg                                                     <  1:956107/239‑1 (MQ=255)

                                                                                                                                                                    ttgtCACGGGGGAAAAAAATAAAAAACCAC‑ATTTT‑TAGTGTTAGTTTGGGGCGG‑TAAATTTTTC‑CAGACAAAA‑ACAAAAACGGGATTTGCA‑ATGAGGAGCGAAAGAAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGATAACTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                 <  1:134118/236‑1 (MQ=255)

                                                                                                                                                                    tatTCGCGGGGACAAAAAATATAAAACCAC‑ATTTT‑TAGGGGTATTTTGTGGCGG‑TAAATTTTTC‑CAGACAAAT‑ACAAAAACGGGAGTTGCC‑ATGATGAGCAAAAGTAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                 <  1:884414/239‑1 (MQ=255)

                                                                                                                                                                    tatTCAGGGTGGAAAAAAAAATAAAACCAC‑ATTTT‑TGGTGGAAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑AAAAAACCTGGATTTGCC‑ATGAGGAGCGAAAGAAATAAG‑AAACGGGTAGGGAATAAGT‑TGAAAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                 <  1:1120739/239‑1 (MQ=255)

                                                                                                                                                                     atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTT‑CAGACAAAT‑ACAAAAAAATGAGTTGCC‑ATGATGAGCGGAAAGAATAAA‑CAACAGGCAGTGAATAAGA‑TTGCAAAGATTGTCGCTAACTTTACCGCCACGATTTTTTCCCCAATGCCCGATGACGGTGTGGGTAAACTAAAACAACTAAAAGATGCCGCAACGACGTCGATggggga                                                >  1:816373/1‑237 (MQ=255)

                                                                                                                                                                     atTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTT‑CAGACAAAT‑ACAAAAAAAGGAGTTGCC‑ATGATGGGGGGAAGGAAAAAG‑CAAAAAGCCGGGAATAAAT‑TGACAAAGAATGTTGCTTACCTTTCCCCCCTGATTTTTACCCCAAAGCCTGATGACGTGGGGGGGAAAATAAAACAACTAAAGGATACCCAAACGACCTCGCTggggga                                                >  1:385551/1‑237 (MQ=255)

                                                                                                                                                                         gggtggaaaaaaaaTAAAAAACCAA‑TTGTG‑TAGTGGGTGTTTGGGGGTG‑ATAATTTTCC‑CAAACAAAT‑AAAAATAAGGGATGTGCA‑AGAATGGACAAAATAAATAAG‑AAACGGGTAGTGAAAATGT‑TGACAGAGTTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                            <  2:741636/231‑1 (MQ=255)

                                                                                                                                                                         agggTGGAAAAAAATA‑AAAACCACAATTTT‑TAGGGGTATTTTGGGGCGG‑TAAATTTTCC‑CAGACAAAT‑ACAAAAAGTGGAGTTGCC‑ATGATGAGGGAAAGTAATAAG‑CAACAGGAAGTAAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATACCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                            <  1:1229995/237‑1 (MQ=255)

                                                                                                                                                                           ggTGGAAAAAAATAAAAACCCAC‑ATTTT‑TGGTGGTTGTTTGGGTCGG‑TTAATTTTCC‑CAAAAAAAT‑AAAAAAACTGGATTTGCC‑ATGATGAGCAAAAGTAATAAG‑CAACAGGCAGTAAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                          <  1:484054/239‑1 (MQ=255)

                                                                                                                                                                            gtagaaaaaaaTAAAAAACCCC‑ATTTT‑TAGTGGAAGTTTGTGGCGG‑TGAATTTTAC‑AAGAAAAAA‑AAAAAAACTGGAGTTGCC‑AGGATGAGCAAAAGTAAAAAG‑CAACAGGCAGTAAATAAGT‑TGACAGAGTTTGTCGGTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                          <  1:515855/233‑1 (MQ=255)

                                                                                                                                                                             gggaaaaaaaTAAAAACCCAC‑ATTTT‑TAGTGGTAGTTTG‑GGCGGTTGATTTTTAC‑AAGAAAAAT‑CAAAAAACGGGGTTTGCC‑ATTATGAGCGAAAGTAATAAG‑CACCAGGAAGTGAAAAAGT‑TGACGGGTATTGTGGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                          <  1:418404/233‑1 (MQ=255)

                                                                                                                                                                                   aaaaTAAAAACCCCT‑ATTTT‑TGGTGTTATTTTGGGGCGT‑GTAATTTTTC‑AAGAAAAAT‑ACAAAAACTGGAGTTGCC‑TTGAGGAGCGAAAGTAATAAG‑CAACAGGCAGTTAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                  <  1:696782/239‑1 (MQ=255)

                                                                                                                                                                                    aaaTATAAAAAATC‑ATTTT‑TGTTGTTTGTTTTTGGGGG‑GTAAATTTTC‑CAAACAAATAAAAAAAAGTGGGGTGACC‑ATGGGGAGGAAAAAAAAAAAG‑ACACGGACAGTAAATAAGT‑GGAAAGAGATTGCCGCTATTTTACCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                  <  2:385551/239‑1 (MQ=255)

                                                                                                                                                                                     aaTATAAACCCAC‑TTTTT‑TATGGTTATTTTGGGGCGG‑TGATTTTTCC‑CAGACAATG‑AAAAAACCGGGAGTTCCC‑AGGAGAAGGAAAAGTAATAAG‑CAACAGACTGTGATTAAGT‑GGACAGATATGGCGGTTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                <  1:517384/239‑1 (MQ=255)

                                                                                                                                                                                     aaTAAAAAACAAA‑ATTTG‑TAGGGGTAGTTGGTGGCGG‑TGAATTTTCA‑CAGAAAAAT‑ACAAAACATGGGGTGGCC‑ATGAGGAGGGAAAGTAATAAG‑CAACAGGCAGTTAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                <  1:969570/239‑1 (MQ=255)

                                                                                                                                                                                                          aGTGGTAGTTTGGGGCGG‑TGAATTTTCC‑CAGACAAAT‑ACAAAAACTGGATTTGCC‑AGAATGAGCAAAAGTAATAAG‑CAACAGGCAGTGAATAAGT‑TGAAAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                          <  1:828842/210‑1 (MQ=255)

                                                                                                                                                                                                                aGTTTGTGTGGG‑TAATTTGTTC‑CAGAAAAAT‑AAAAAAATGGTGGTTGCA‑AGGAGGAGCGAAAGAAAGATC‑AAAATGTCGGTGAAAAGGTGTGA‑AGTGTTTGTCGCAATGTTTCCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa       <  2:276076/239‑1 (MQ=255)

                                                                                                                                                                                                                aGTTTGGGGCGG‑TAAATTTTTC‑AAAACAAAT‑ACAAAAACTGGAGTTGCA‑AGGAGGGGCAAAAGTAATAAG‑CAACAGGAAGTAAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa       <  1:925957/239‑1 (MQ=255)

                                                                                                                                                                                                                 gTTTGGGGCGG‑TAAATTTTCC‑GAAAAAAAT‑ACAAAAACGGGATTTGCC‑AGGAAGAGCAAAAGTAAAAAG‑CAACAGGAAGTGAAAAAGT‑TGAAAGAGTTTGCCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAt      <  1:893312/239‑1 (MQ=255)

                                                                                                                                                                                                                     gtgGCGG‑TGAATTTTTC‑CAGAAAAAT‑ACAAAAACTGGAGTTGCC‑ATGAGGGGGAAAAGTAATAAG‑CAACAGGAAGTGAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc  <  1:1024431/239‑1 (MQ=255)

                                                                                                                                                                                                                     ggggCGG‑T‑AATTTTTCACAGAAAAAC‑A‑AAAAACTGGTATTGCCAATGAGAGGCAAAAGTAATAAG‑CAACGGGCAGGAAATAAGT‑TGACGGGGATGGCCGTTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc  <  1:1181739/237‑1 (MQ=255)

TTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTT‑TAGTGGTAGTTTGTGGCGG‑TGAATTTTTC‑CAGACAAAT‑ACAAAAACTGGAGTTGCC‑ATGATGAGCGAAAGTAATAAG‑CAACAGGCAGTGAATAAGT‑TGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAC  >  NC_000913/3206208‑3206657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A