Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 177 96.4 2178490 87.4% 1904000 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,274,349 T→G 26.1% G57G (GGT→GGG)  yejB → microcin C ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,3490TG26.1% 122.8 / 14.4 75G57G (GGT→GGG) yejBmicrocin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (15/38);  new base G (20/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 5.31e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTCC  >  NC_000913/2274115‑2274586
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
acccTGGGGGTAGGACGTCAATAAAGCGGCCAACCGGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGtt                                                                                                                                                                                                                                             <  1:643300/236‑1 (MQ=255)
    tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGATTACCCGGCGCAGGCGGTGAAGGGGtttggtc                                                                                                                                                                                                                                        >  1:535882/1‑234 (MQ=255)
      gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGTTTCGTgcc                                                                                                                                                                                                                                       >  1:934641/1‑238 (MQ=255)
      gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGTTTACCCGGCGCAGCCGGGGAAAGGGTTCGGgcga                                                                                                                                                                                                                                      >  2:261104/1‑237 (MQ=255)
          tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGCCCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCCCGGGGGGGGAAGGGGTTCGGggcagccc                                                                                                                                                                                                                                  >  2:284657/1‑238 (MQ=255)
          tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGATTACCCGGCGCAGGCGGGGAAGGGTGTCGGgccaccca                                                                                                                                                                                                                                  >  1:319779/1‑239 (MQ=255)
            tGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTAAGTTTGGGATTGCCGGGGAATTACCCGGCGCAGGCGGTGAAGGGGTTCGGgcccgccagg                                                                                                                                                                                                                                >  2:570086/1‑237 (MQ=255)
                gTCAATAAAGCTGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGCAATGCCGGGGTATTACCCGGCGCAGGCGGTGAAGGGTGTCGTgcccggcacgacca                                                                                                                                                                                                                            >  1:480170/1‑228 (MQ=255)
                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGTTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAAGGCCGGAGAATTACCCGGGGCAGGCGGTGAAGGGGTGCGTGCCAGCCATGCGCa                                                                                                                                                                                                                            >  1:118184/1‑239 (MQ=255)
                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGCGTATTACCCGGCGCAGGCGGGGAAGGGGTTTGGGCCCGCCATggcca                                                                                                                                                                                                                            >  1:369472/1‑235 (MQ=255)
                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCa                                                                                                                                                                                                                            >  1:190821/1‑239 (MQ=255)
                    aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCCCAGGCGGTGAAGGTGTTCGGGCCAGCCATGCGCaaaag                                                                                                                                                                                                                        >  1:683531/1‑237 (MQ=255)
                            gCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCCGGACCAAACGGgggtat                                                                                                                                                                                                                 >  1:719339/1‑236 (MQ=255)
                            gCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGCCGCAGGCGGGGAAGGGGTTCGTTCCAGCCATGACCAAACGGGTGTCg                                                                                                                                                                                                                 >  1:512385/1‑238 (MQ=255)
                             ccAAACTACCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGAggcggc                                                                                                                                                                                                               >  1:337722/1‑239 (MQ=255)
                                      cGTCCGCCAGCAAACGGGGGGAGTAGTTGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACTTTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAg                                                                                                                                                                                                       <  2:512385/238‑1 (MQ=255)
                                         cccccggCAAAGGGGGAGTGGAGATGGGCGCTTACCTGATTCGCGGTCTGGTGCGGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTGCATCGTGCAAATGGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                                   <  2:727503/233‑1 (MQ=255)
                                         ccGCCAGCAAACAGGGGGGTGAGATGGGCGTTTACCTGATTCGCCTTCTGTTTCTGGGCGTCCCAACCTTATGGGCGATTATCCCCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGGGGTCCGGTCGACCAGGCCACCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                                   <  2:49215/239‑1 (MQ=255)
                                         ccGCCAGCAAACAGGGAAAGTAGAGTGGCTCTTACCTGATTCGCCGTCTTTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    <  1:78684/238‑1 (MQ=255)
                                                       ggAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCCGGCGGTGAAGGGGTTCGGTCCAGCCATGCGCCAACGGGGGGTGGCAAAATCACGGACAGGAATTTCCGTgg                                                                                                                                                                                     >  1:1011819/1‑239 (MQ=255)
                                                         agagTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCGCAGGCGGGGAAGGGGTTCTGGCCAGCCCTGCGCAAAAGGGGGTGGGCCATATCAGGGACAGTAATTTCCATGGCg                                                                                                                                                                                   >  1:70584/1‑239 (MQ=255)
                                                          gagTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATTTCAGCGCCAGTAATTACCATGGCgg                                                                                                                                                                                  >  1:640738/1‑239 (MQ=255)
                                                              ggATGGGGGCTTCCCTTATCCCCCGTCTGTTGTGTGTGATCCCAACATTGGGGGCGATTACCCCCATCAAC‑TTTTTCATCGTGCAAATGGCGCCTGGCGGTCCGGTCGCCCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                               <  2:735191/237‑1 (MQ=255)
                                                              atgtGGGCTTTTACCTGATTCGCCTTCTGTTGCTGGGTACCCCAAAATTAGGGGCGATTTCCACCATCAAC‑TTTTTCCTCGTGCAAATTGCGCCTGGGGGGCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                               <  2:169632/235‑1 (MQ=255)
                                                               gATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGCGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGGGGCCGCCCTGCGCAAACGGGGGTCGGCAATATCAGGGACGGGAATTACCCTGGCGGAagcg                                                                                                                                                                             >  1:488522/1‑239 (MQ=255)
                                                               gATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCCTGGGCAAACGGGGGTCGGCAAAATAAGCGACAGTAATTACCGgggggggacgg                                                                                                                                                                             >  2:927980/1‑234 (MQ=255)
                                                                 tGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGGAGGTGTTCGTGCCAGCCATGGGCAACCGGGTGGCGGCAATATTAGCGCCAGGAATTTCCGGCGCGGAcggcgg                                                                                                                                                                           >  1:400046/1‑235 (MQ=255)
                                                                   gggctttACCTGTTTCGCCTTTTGTTGCTGGTGACCCCAACATTAGGGGCGATTACCCCCATCAAC‑TTTTTCAGCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                         <  2:934641/234‑1 (MQ=255)
                                                                    gcccTTACCGGATTCCGCGTCTGGTGGTGGGGATCCCAACATTATGGGCGATTATCACCAGCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                         <  1:992711/235‑1 (MQ=255)
                                                                     cgcTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACCGGTGTCGGGAATATACACGACAGGAATTACCGTGGGGGGCCCgggatag                                                                                                                                                                       >  2:49567/1‑234 (MQ=255)
                                                                       cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGGCAGTAATTACCGGGGGGGACGCGGGTTTGa                                                                                                                                                                      >  1:735191/1‑238 (MQ=255)
                                                                                 tCGCCTTCTGTTGCGGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGa                                                                                                                                                           <  1:284657/239‑1 (MQ=255)
                                                                                   cgggtttgttgttgtGGATCCCAACATT‑TGGGGGGTTTTACCCCACAACTTTTTTCACGGGGAAATTTGCGCCGGGGGGTCCGGTCGCCCGGGCCAGCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATc                                                                                                                                                         <  2:1064000/233‑1 (MQ=255)
                                                                                      ttttttttcTGTTGATCCCAAAATTAGGGGCGATTACCCCCATCAAC‑TTTTTCATCGTGCAAATTGGGCCTGGCGGCCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCt                                                                                                                                                      <  2:190821/231‑1 (MQ=255)
                                                                                      gttgtgttttGGTGATCCCAACTTTATGGGCGATTATCCCCATCACC‑TTTGTCATCGTGAAAATTGCGCCTGGGGGTCCGGTCGGCCCGGCCAGCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCt                                                                                                                                                      <  2:480170/230‑1 (MQ=255)
                                                                                                 gTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTAACGTGGCGGACGCGGGTTCGAGTCAGAAGGGATcccgtggataa                                                                                                                                               >  1:1064000/1‑225 (MQ=255)
                                                                                                      ccgaaCATTATGGGCGATTATCTCCATCAAC‑TTTTTCTTCGTGCAAATTGCGCCTGGCGGTCCGGCCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAACGTGTTCGTGCCATCCATGCGCAAACGGGTGTCGGCAATATCAg                                                                                                                                                                                                       <  1:359259/171‑1 (MQ=255)
                                                                                                       ccAACATTATGGGCGTATATCCCCATCAAC‑TTTTTCCTCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTa                                                                                                                                     <  1:105652/239‑1 (MQ=255)
                                                                                                                tGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATAAGCGCCAGTAATTACCGGGGCGGACCCGGGTTAGATCCAGAAGGGATCGGTTGGATCCACCAACGCTACGGGTTTgg                                                                                                                            >  1:1023512/1‑238 (MQ=255)
                                                                                                                     tcttttccccATAAAT‑TTTTTCATCTGGAAAATGGGGCCGGGCGGTCCGTGCGCCCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAg                                                                                                                        <  2:932694/230‑1 (MQ=255)
                                                                                                                     gATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCg                                                                                                                                                                                                     >  1:197081/1‑161 (MQ=255)
                                                                                                                     gATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCg                                                                                                                                                                                                     <  2:197081/161‑1 (MQ=255)
                                                                                                                                    c‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    <  1:465087/147‑1 (MQ=255)
                                                                                                                                      ttttcgagcGTGCAATTGGGGCGTGGCGGTCCGGGCGACCCGGCCATGGCCGACTTTGAGTTTGGTAATGCCGGAGTATTACCCGGCCCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTa                                                                                                      <  2:10668/231‑1 (MQ=255)
                                                                                                                                               gTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAGATCCAGAAGGGATCGCTGAGATCCCTCATCGCTACCGGTTTTATAAGCCG‑ATCCACGAACGTTACTTCaaaa                                                                                              >  1:847846/1‑238 (MQ=255)
                                                                                                                                                tGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCCAACGGGTGTCGGCAATATCAGCGGCCGTAATTAACGGGGCGGGCGGCGGTTAGATCCAGAAGGGATACCTGTGATACCCCCTCCCTACGGGTTTTATAAACCGCATCC‑CGCACGTTTCTTCaaaaa                                                                                             >  2:245730/1‑237 (MQ=255)
                                                                                                                                                    aaTTGCGGCTGGGGTTCCGTTCGACCGGGTACGCGCCGCCATGGAGTTTGGTAATGCCGGAGGATTGCCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGctct                                                                                        <  2:734814/239‑1 (MQ=255)
                                                                                                                                                    aaTTGCGCCTGGCGGGCCGGTCGACCAGGCATTCCGCGGCATTGAGTTGGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTTAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGctc                                                                                         <  1:898633/238‑1 (MQ=255)
                                                                                                                                                    aaTTCCGCCTGGCGGTCCGGTTGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGAAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGctct                                                                                        <  1:808671/239‑1 (MQ=255)
                                                                                                                                                    aaTGGCGCCTGGCGGTCCGGGCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGctct                                                                                        <  1:334780/239‑1 (MQ=255)
                                                                                                                                                                cggtccggtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGATTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATc                                                                             <  1:880054/238‑1 (MQ=255)
                                                                                                                                                                                tccATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCg                                                             <  1:458332/237‑1 (MQ=255)
                                                                                                                                                                                  cttccccgccATGGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGGGGTTAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACCATAATTACCGTTGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTGGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATa                                                          <  2:1009381/234‑1 (MQ=255)
                                                                                                                                                                                  aagcgccgcCATTGAGTTTGGTAATGCCGGATTATGACCCGGCGCAGGCGGTGAAGGTGTTCTTGCCCGCCATGCGCAAACGGGTTGCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTGTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATa                                                          <  1:902679/236‑1 (MQ=255)
                                                                                                                                                                                          ccATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAGACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAAAAGTGATCGCTGAGATCACTCATCGCTACGGGTTTGTTAAGCCG‑ATCCACGAACGGTACTTCCAAATGCTCTGGGACTCCATCCCCTTTGATTTTGGCGATAGCCTGtt                                                   >  2:421883/1‑238 (MQ=255)
                                                                                                                                                                                              tGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCCAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCCCa                                              >  1:921623/1‑239 (MQ=255)
                                                                                                                                                                                              gtggTTTTGTAATGGCGGAGTATTTCCCGGCGCAGGCGGTGAAGGTGTGCGGTCCCGCCATGCGCAAAAGGGTGACGGCAATATCAGGGACAGTAATTACCGTGGCGGACGCGGTTTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCa                                              <  2:719339/236‑1 (MQ=255)
                                                                                                                                                                                               gggTTTGGTAATGCCGGAGTTTTACCCGGCGCGGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAg                                             <  2:488522/237‑1 (MQ=255)
                                                                                                                                                                                               gAGTTTGGTTATGCCGGGGTATTACCCGGCGCGGGCGGTTAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATAGCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAg                                             <  2:319779/239‑1 (MQ=255)
                                                                                                                                                                                                    tGGTAATGGCGGAGTATTTCCCGGGGGAGGCGGTTAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGGAATATCAGCGACAGTAATTGCCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                                        <  1:582097/239‑1 (MQ=255)
                                                                                                                                                                                                               gggttttACCCGGGGCCGGGGGTGAAGGTGTGCGTGCCCGCCGTGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACg                             <  2:650862/234‑1 (MQ=255)
                                                                                                                                                                                                               gAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACCGTAATTACCGTGGCGGACGCGGGTTTGGTCCAGAAGGGATCGCTGGGATCACTCATCGCTACGGGTTTGATAAGCCG‑ATCCACGAACCGTACCTCAAAATGCTCTGGGGCTAAATCCGCCTTTGTTTTGGCGCTGACCTGTTTTCCAGGGCCCCGGGGCTggc                              >  2:135032/1‑236 (MQ=255)
                                                                                                                                                                                                                aGTATTCCCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACg                                                                                                             <  1:611198/158‑1 (MQ=255)
                                                                                                                                                                                                                aGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAACGACACGGTGCTGAc                              >  1:373009/1‑237 (MQ=255)
                                                                                                                                                                                                                 gttttccccGGGGCGGGGGGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                            <  2:746617/232‑1 (MQ=255)
                                                                                                                                                                                                                 gTATTTCCCGGCGCAGGCGGTGAAGGTGTTCGGGCCAGGCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                            <  1:570086/238‑1 (MQ=255)
                                                                                                                                                                                                                 gTATTACCCGGCGCCGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                            <  1:809064/238‑1 (MQ=255)
                                                                                                                                                                                                                     tACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    >  2:471596/1‑67 (MQ=255)
                                                                                                                                                                                                                     tACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    <  1:471596/67‑1 (MQ=255)
                                                                                                                                                                                                                            cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGATCCAGAAGGGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGGGATAGCCCGTTTTGCAGGGCCCCGGGGCGGACGCTGATTTAAGACa                >  1:144607/1‑239 (MQ=255)
                                                                                                                                                                                                                            cgcAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACCCGGGTTAGATCCAGAAGTGATCCCTGAGATCACCCATCGCTACGGGTTTGATAAGCCG‑ATCCACCAACGTTACTTCAAAATGCCCTTGGACTAACTCCGCTTTGTTTTTTGCGGAAGCCCGTTTTCCACGGCCCCGGTGCTGACGCCGTTTaaaaaaa                >  2:72009/1‑235 (MQ=255)
                                                                                                                                                                                                                            cgcAGGCGGAGAAGGGGGTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGCCGCGGATTAGATCCAGAAGTGATCGCTGAGATTCATCAACGCTACGGTTTTGAAAAACCG‑AATCACGAACCGTACTTCAAAAAGCGTTTGGACTACACCCGCCTTGATTTTTGTGATAAACCGTTTCTCGCGGCCCCCGTGCTGAGGCTGATTAAAAACa                >  2:834264/1‑239 (MQ=255)
                                                                                                                                                                                                                                  cGGGGAAGGTGTTCGTGCCAGCCCTGCCCAAACGGATTTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGAGCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTg           <  2:369472/238‑1 (MQ=255)
                                                                                                                                                                                                                                   ggTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGc          <  1:730400/238‑1 (MQ=255)
                                                                                                                                                                                                                                     tGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCg        <  1:772572/238‑1 (MQ=255)
                                                                                                                                                                                                                                           tgtTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTcc  >  1:85701/1‑238 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAC‑TTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCG‑ATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTCC  >  NC_000913/2274115‑2274586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A