Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F9 I0 R1 83 124.0 2465218 96.8% 2386331 230.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,151,689 G→T 38.6% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6890GT38.6% 13.4 / 10.0 34intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (14/6);  new base T (6/7);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACC‑ATTGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG  >  NC_000913/2151496‑2151925
                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
tGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAAC‑ATTTATTCCTTTTTTGTCACCCAATCCAAAA‑AAGAAAGCGACGAGC‑TAAAAGGAAAAATAATTTGCTTA‑TCAACC‑GGTTTATTTTTTTTTTTAAATTGGGGGTTTGATTTTTTTT‑TT‑TAA‑CACCCCGTAAAAAACACACTTTCCCcacacccagcg                                                                                                                                                                                                    >  1:477713/1‑231 (MQ=255)
              aaaaCAAAAAAGAAGATATTTCAGCCTGGATGTATACGCGGGGGGATAAAATTTACGGCGTTTATCCC‑ATTGATCCTTTATTGGTCC‑CCTACCCAAAAGAAGAAGCCGACGAGC‑TAAGAGAAAAATTAGTTCGCTAA‑TCACCC‑GTTATA‑TTTATTGTTTAAACTGGCGTTTGATTTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                                                                                                                                   <  1:436470/239‑1 (MQ=255)
                    aaaaaaaaGATATTTCAGCTGGGATTTATGCGGGGGGGAAAAAAATTACCGGGGTTTATACC‑ATTACTCTTTTTTGGTTCC‑CCTAACAAAAAGAAAAAGCCGAGGGGA‑AAAGAAAAAAATTTGTTCGCT‑A‑TCCGCCTTTTATA‑TTTTTTGTTAAAACGGGGGTTTAATTTGTTGTT‑TC‑AAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                                                                                                             <  2:1077744/237‑1 (MQ=255)
                                   cagatggtttGTATCCGCGGGGGGATAAAATTTGCGGCGTTTATACC‑ATTTATCTTTTATTGTTCC‑CCTATCAAAAAGAAGAAGCCGACGAGA‑TAAGAGAAAATTTAGTCCGTAAC‑TCAGCC‑TTTATT‑TTTTTTTTTTAACCGGGCGTTTGATTTTTTTTC‑TA‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccg                                                                                                                                                              <  2:477713/230‑1 (MQ=255)
                                                  cgcgggGGAAAAAAATTAAGGGGGTTTATACA‑ATTGACCTTTTATGGTCCC‑CCTACCAAAAAAAAGAAGCCGAGGAGC‑TAAGAG‑AAAATTTATTCCCTAATTCAGCC‑TTTATT‑TTTTTTTTTAAAACGGGGGGTTAATTTTTTGTC‑TC‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa                                                                                                                                               <  1:653900/239‑1 (MQ=255)
                                                                  ttACGG‑GGTTTTACCAATTGTCCTTTTATTGGCCC‑CCTA‑CCAAAAAAAAGAGCCCAAGAACATAAAACAAAATTTAGTCCGTAAA‑TCAGCT‑TTTTTA‑TTTTTTTTTAAACCGGGGGGTTAATTTTTTTTC‑TC‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                                                                                                               <  1:614736/239‑1 (MQ=255)
                                                                                     tGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTTATCATCCGTACGCCCGGTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAg                                                                                                                             >  1:463056/1‑223 (MQ=255)
                                                                                     tGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGGAACTCAATCTGCCCACAGAGCCAGTGCCCACGGTTTATCATCATCCGTACGGCCGTTTCACGCCGGGCTGGACAAAAACTGCTTTAACAAATCAGGTAATAAAACGAggtt                                                                                                             >  1:793499/1‑236 (MQ=255)
                                                                                     tGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCAATCTTCCCACAGCGCCCGTGCCAACGGTTTTTCATCCTCCGTCCGCTCGTTTACCGCCGGGCTGGACAAAAACTGCCTTAAAAATTCAGGGAAAAAAACGAgttg                                                                                                             >  1:895467/1‑239 (MQ=255)
                                                                                      gATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTTTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGGTTTATCATCATCCGTACGCTCGCTTACCCCCGGGCTGGACAAAAAACGCTTTAACAATTCAGGGAATAAATCGAgttgt                                                                                                            >  2:1070438/1‑239 (MQ=255)
                                                                                         ccTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAAATGTTGTT‑TT‑CAA‑C‑CGCCAAGAGAAACTCAATCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCATCCGTACGCTCGTTTAACGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                                                                                                           >  1:1048379/1‑237 (MQ=255)
                                                                                            ttATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTTTTTTC‑TT‑CCA‑C‑CGCCATTAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACCGTTTTTCCTTCTCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAACGCTTTAACAATTTAGGGAAAAAATAGAattttttccgc                                                                                                      >  2:604140/1‑234 (MQ=255)
                                                                                            ttATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTCCTGc                                                                                                      >  1:801298/1‑239 (MQ=255)
                                                                                                     a‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTTTT‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCACAGGTTTTTCATCATCCGCACGCTCGTTTACCGCCGGGCTGGGCCAAAACCGGTTTTACCATTCAGGTAATTAATCGAGTTGGTCCTGCGTttctgc                                                                                              >  2:1158122/1‑238 (MQ=255)
                                                                                                     a‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc                                                                                              >  1:492121/1‑238 (MQ=255)
                                                                                                          aTCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGTATTTTTTCC‑TT‑CAA‑C‑CGCCATTAGAAACTCACTCTGCCCCCAGCGCCACGTCCAACCGTTTTTTATCAACCGTACGCCCGCTTTCCGCCCGGCTGGACCAAAAACCCTTTTACAAATCAGGTAATAAATCGAGTTGTTCCTCCGTTGCTgtcgccc                                                                                         >  2:502177/1‑238 (MQ=255)
                                                                                                                     gaagCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTTAATTGTTGAC‑TT‑TAA‑C‑CGCCATGAGGAAATTACTCTGCCCACAGGGTCAGTGCCAACGTTTTATCATCATCCGTTCGCTCGTTTACCGCCGGGGTGGACAAAAAATGCTTTTACAATTCATGGACTAAATCGAGTTTTTCCTGCGGTGCTTGCTCCCGTCCATTGata                                                                              >  2:252931/1‑237 (MQ=255)
                                                                                                                      aagCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTTTTTTC‑TT‑TAA‑A‑CGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAACGTTTTATTATCATCCGCAAGCCCGTTTACCCCCGGGCTGGACACAAAATGCATTATAAATTTAGGGAATAAAACGAGTTGTTTCTGCGTTGCTGCCGCCAGTCCAtttcaaa                                                                             >  2:808528/1‑234 (MQ=255)
                                                                                                                       agCCGACGAGT‑AAAAAAAAAATTTAGTCCTTTAA‑CCACCC‑TTTATT‑TTTTTTTTTTAATCGGGC‑GTTTAATTTTTTTC‑TTCCAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                            <  1:604140/239‑1 (MQ=255)
                                                                                                                                   tAAAAGAAAATTTTGTTCG‑TTA‑TCATCC‑GTTTTTTTTTATTGTTCAGACGGGCGTTTGATTTGTTGTC‑TC‑CAC‑C‑CGCCATGAGAAACTCACGCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGTCAGTCCATTGAAAAACGGGGTaaaga                                                                 <  2:401509/239‑1 (MQ=255)
                                                                                                                                            aaTTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATTAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGGCCATTTAAAAAAGGGGTAAAGAAAGGca                                                           >  1:858271/1‑236 (MQ=255)
                                                                                                                                                 gTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTTTTGTT‑TT‑CCA‑C‑CCCCATGAGAAACTCACTCTTCCCCCAACCCCCGTGCCCACGTTTTTTCCTCATCCGTACGCTCGTTTTCCCCCCGGCCGGACAAAAAATTCCTTTACAATTTAGGTAATTAAACGAGGTGTTCCTTCGTTGCTTCCGCCCATCCCTTTAAAAAAAGGGGAAAGAAAAGCACATgaggg                                                   >  2:1073900/1‑238 (MQ=255)
                                                                                                                                                  ttCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑TCA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCACCGTTTTATCATCATCCGTACTCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCCAGTTGTTTCTGCGTTTCTGCCGCCCGTCCCTTGGAAAAAGGGGGTAAGACAGGCACCTgagggg                                                  >  2:863741/1‑237 (MQ=255)
                                                                                                                                                      cctaaataGCC‑TTTTTT‑TTTTTTGTTTAAACCGGGGGTTTAATTTTTTTC‑TC‑CAACC‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                                                <  2:343433/232‑1 (MQ=255)
                                                                                                                                                              gCC‑TTTATA‑TTTTTTGTTAAAACTGGGGGTTGAATTGTTGTC‑TA‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                       <  1:1113816/239‑1 (MQ=255)
                                                                                                                                                               tctttttta‑ttttttGTTAAACCTGGTGTGTGATTTTTTTTT‑TA‑CAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                       <  1:140672/232‑1 (MQ=255)
                                                                                                                                                                cctttATA‑TTTTTTTTTTAAAGTGGCGGTTAATTTTTTGTC‑TC‑AAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                      <  1:944003/236‑1 (MQ=255)
                                                                                                                                                                        tttttttGTTTAACGTGGGGTTTGATTTGTTTTC‑TC‑AAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTaa                               <  1:863741/238‑1 (MQ=255)
                                                                                                                                                                           tattGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAAATCAATCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCCCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTAACAATTTAGGTAATAAATCGAGGTTTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAACGGGGTAAAGAAAAGCACATGAGGGGAATTATCCAGCATATTTaaag                             >  2:514476/1‑236 (MQ=255)
                                                                                                                                                                            attGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑TAA‑C‑CGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATTCGTAAGCTCGTTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTTAGGAAATAAATAGAGTTGTTCCCGCGTTGCTGCCGCCAGTTCATTCAAAAAAGGGGTAAAGAAAGGCACATGAGGAGGACTATTCCGCATCTTTAACGc                            >  1:60681/1‑238 (MQ=255)
                                                                                                                                                                                  tAAACTGGCGGTTGAAATGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTTAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAAAGCCAGTc                       >  1:442125/1‑237 (MQ=255)
                                                                                                                                                                                      cggcgggTTAAATTTTTCTC‑TC‑CAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCAGCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                 <  1:1158122/234‑1 (MQ=255)
                                                                                                                                                                                       ggggtgTTTAATTTTTTTCTTT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                 <  1:302663/234‑1 (MQ=255)
                                                                                                                                                                                          cGGGTGAATTTTTGTC‑TT‑CAA‑A‑CGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCCTACGCTCGCTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTTCTGCGTTGCTGCCGCCCGTCCCTTGAAAAACGGGGTGAAACAAAGCACATAGGGGGAATTCTCCAGCATATTTAACGCCAATTGCTgggaacc             >  2:470340/1‑234 (MQ=255)
                                                                                                                                                                                                     ttgtC‑TA‑CAC‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtg  <  1:514476/239‑1 (MQ=255)
                                                                                                                                                                                                       gtC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATCCCATCCGTAAAt   >  2:406874/1‑236 (MQ=255)
                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
TGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACC‑ATTGATCCTTTATTGGTCA‑CCTATCCAAAAGAAGAAGCCGACGAGC‑TAAGAGCAAAATTAGTTCGCTAA‑TCAGCC‑GTTATA‑TTTATTGTTTAAACTGGCGGTTGAATTGTTGTC‑TT‑CAA‑C‑CGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATG  >  NC_000913/2151496‑2151925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A