Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F36 I0 R1 80 95.4 3476014 97.7% 3396065 133.8

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCA  >  NC_000913/2173220‑2173500
                                                                                                                                               |                                                                                                                                         
GATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATG                                                                                                                                  >  NB501016:230:HFVJLAFXY:4:21410:16646:13853/1‑151 (MQ=60)
                   GACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGA                                                                                                               <  NB501016:230:HFVJLAFXY:3:21408:6476:6184/151‑1 (MQ=60)
                        CCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACATCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCTATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAG                                                                                                          <  NB501016:230:HFVJLAFXY:1:21105:10146:5184/151‑1 (MQ=60)
                         CATCGCCACGAAGAAGCCGATGGTGGCAAGATAGCCAAACGGCAGCACATGATTACCCGGCACACAATCAGCAATTAAAATGGTGAGTGGGATAAAAATAAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGG                                                                                                         >  NB501016:230:HFVJLAFXY:3:11606:2146:3003/1‑151 (MQ=60)
                              CCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAT                                                                                                    >  NB501016:230:HFVJLAFXY:1:21310:16416:1641/1‑151 (MQ=60)
                              CCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAT                                                                                                    <  NB501016:230:HFVJLAFXY:3:11411:5794:10664/151‑1 (MQ=60)
                                ACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCA                                                                                                  <  NB501016:230:HFVJLAFXY:1:21212:22532:13249/151‑1 (MQ=60)
                                     cgtggCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAC                                                                                             <  NB501016:230:HFVJLAFXY:4:11406:4666:20372/146‑1 (MQ=60)
                                         CCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCCGGCTAGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCACCGCCGGATCAAGGCCAATCAGGAACT                                                                                            >  NB501016:230:HFVJLAFXY:2:21210:18926:16352/1‑148 (MQ=60)
                                         CCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCA                                                                                                            >  NB501016:230:HFVJLAFXY:2:21310:12796:1279/1‑132 (MQ=60)
                                                         CGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAG                                                                                                                         <  NB501016:230:HFVJLAFXY:2:11106:22277:16877/103‑1 (MQ=60)
                                                          GCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCC                                                                        <  NB501016:230:HFVJLAFXY:3:21602:1356:14464/151‑1 (MQ=60)
                                                                 GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAC                                                                 <  NB501016:230:HFVJLAFXY:2:21208:23269:12212/151‑1 (MQ=60)
                                                                      CACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACT                                                             >  NB501016:230:HFVJLAFXY:2:21206:6446:3794/1‑150 (MQ=60)
                                                                        CCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAGCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACG                                                          <  NB501016:230:HFVJLAFXY:1:21212:16129:13843/151‑1 (MQ=60)
                                                                                CCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAC                                                                              >  NB501016:230:HFVJLAFXY:1:21212:14939:18009/1‑123 (MQ=60)
                                                                                       CACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAT                                           <  NB501016:230:HFVJLAFXY:3:21410:22010:2583/151‑1 (MQ=60)
                                                                                                    TAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGT‑CCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGCGCCTGCTTAGCGATGGGCGTTAAACCAT                             >  NB501016:230:HFVJLAFXY:4:21608:23760:15311/1‑151 (MQ=60)
                                                                                                     AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCAT                             <  NB501016:230:HFVJLAFXY:4:11602:19017:2577/151‑1 (MQ=60)
                                                                                                     AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCA                              >  NB501016:230:HFVJLAFXY:4:11405:20058:20116/1‑150 (MQ=60)
                                                                                                             GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGCCCGCCGAACTTCGCCTGTAACCGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATG                     >  NB501016:230:HFVJLAFXY:1:21205:18951:4792/1‑151 (MQ=60)
                                                                                                             GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGAT                      <  NB501016:230:HFVJLAFXY:3:21612:7577:12070/150‑1 (MQ=60)
                                                                                                                 GGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTT                 <  NB501016:230:HFVJLAFXY:1:11302:25805:5050/151‑1 (MQ=60)
                                                                                                                     TAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATG                        >  NB501016:230:HFVJLAFXY:2:21301:23491:13461/1‑140 (MQ=60)
                                                                                                                             AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTG     <  NB501016:230:HFVJLAFXY:3:21503:12849:5047/151‑1 (MQ=60)
                                                                                                                              GGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGTTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGC                                              <  NB501016:230:HFVJLAFXY:4:11503:11345:2853/109‑1 (MQ=60)
                                                                                                                              GGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGG    <  NB501016:230:HFVJLAFXY:1:11106:14246:18073/151‑1 (MQ=60)
                                                                                                                                 TTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAT                                           >  NB501016:230:HFVJLAFXY:2:11107:10749:2644/1‑109 (MQ=60)
                                                                                                                                  TGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTgattaaacccatcatg  <  NB501016:230:HFVJLAFXY:4:11505:22729:4543/149‑17 (MQ=60)
                                                                                                                                  TGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCA  <  NB501016:230:HFVJLAFXY:3:11606:25412:8196/149‑1 (MQ=60)
                                                                                                                                     CGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCC                                                                        <  NB501016:230:HFVJLAFXY:2:21208:2545:18145/76‑1 (MQ=60)
                                                                                                                                            ccaCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAC                                                              >  NB501016:230:HFVJLAFXY:4:21403:10652:7800/4‑81 (MQ=60)
                                                                                                                                               CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTC                                                                                           >  NB501016:230:HFVJLAFXY:3:21601:13361:4992/1‑49 (MQ=60)
                                                                                                                                               |                                                                                                                                         
GATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCA  >  NC_000913/2173220‑2173500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑