Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F36 I0 R1 111 89.1 3136336 98.1% 3076745 142.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATC  >  NC_000913/2173214‑2173511
                                                                                                                                                     |                                                                                                                                                    
GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGTGATAAAAATCAGGCTTGCCGATACCACCGC                                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:21602:26290:2967/151‑1 (MQ=60)
    CAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGT                                                                                                                                                >  NB501016:230:HFVJLAFXY:2:11312:8808:16262/1‑150 (MQ=60)
           CCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCC                                                                                                                                         <  NB501016:230:HFVJLAFXY:3:21608:9349:6313/150‑1 (MQ=60)
            CACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCC                                                                                                                                         >  NB501016:230:HFVJLAFXY:2:21107:17371:17258/1‑149 (MQ=60)
               catgcacgccgaccgacatacaCACGAAGAAGCCGAAGGTGGCAACATCGCAAAACGGCAGCACCTGATTACCCCGCACACAAACAGCAATTAAAATGGTGAGTGGTATAAAAATCAGCCTAGCCTAAACCA‑‑CCGCCGTATGTCCCAccac                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11509:1095:18357/129‑5 (MQ=60)
                 TGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCCGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGAGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGGCGTATGTCCCAGCAGA                                                                                                                                   >  NB501016:230:HFVJLAFXY:3:21601:11197:10067/1‑150 (MQ=60)
                   CACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAC                                                                                                                                >  NB501016:230:HFVJLAFXY:3:11503:16916:14930/1‑151 (MQ=60)
                   CACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAAGTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAC                                                                                                                                >  NB501016:230:HFVJLAFXY:4:11604:2647:9184/1‑151 (MQ=60)
                      GGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGC                                                                                                                                      >  NB501016:230:HFVJLAFXY:3:21403:10881:6930/1‑142 (MQ=60)
                             GCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCA                                                                                                                       >  NB501016:230:HFVJLAFXY:1:11109:2665:11208/1‑150 (MQ=60)
                              CCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAG                                                                                                                     <  NB501016:230:HFVJLAFXY:2:21312:16662:18764/151‑1 (MQ=60)
                               CATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGG                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11202:18469:2444/151‑1 (MQ=60)
                                      ACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATC                                                                                                              >  NB501016:230:HFVJLAFXY:1:11312:2828:19076/1‑150 (MQ=60)
                                       CGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACACACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATC                                                                                                              >  NB501016:230:HFVJLAFXY:1:21211:23237:18388/1‑149 (MQ=60)
                                           GAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGAAACCA‑‑CCGCCGTAAGTCCCAGCAGCAACGCCG                                                                                                                            >  NB501016:230:HFVJLAFXY:2:21107:13314:13568/1‑131 (MQ=60)
                                             AGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTC                                                                                                      >  NB501016:230:HFVJLAFXY:4:21612:5462:16377/1‑151 (MQ=60)
                                                         CAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCCATCAGGAACTCCTGACCGCCGA                                                                                           >  NB501016:230:HFVJLAFXY:2:21309:9186:9372/1‑150 (MQ=60)
                                                                            CACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACG                                                                           <  NB501016:230:HFVJLAFXY:1:21204:9687:2579/147‑1 (MQ=60)
                                                                                TGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATACAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACG                                                                     >  NB501016:230:HFVJLAFXY:1:21202:8653:18982/1‑149 (MQ=60)
                                                                                     ACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGC                                                              >  NB501016:230:HFVJLAFXY:2:21107:12292:6038/1‑151 (MQ=60)
                                                                                      CCCGGCACACAAACAGCAATTAAAGTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACAACGAGCCTGCT                                                             >  NB501016:230:HFVJLAFXY:4:11601:11498:16406/1‑151 (MQ=60)
                                                                                                AAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGG                                                   <  NB501016:230:HFVJLAFXY:1:11206:23831:13263/151‑1 (MQ=60)
                                                                                                   CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCCGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGT                                                >  NB501016:230:HFVJLAFXY:2:11212:24693:11172/1‑151 (MQ=60)
                                                                                                   CAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACGCCTGACCGCCGAACTTCGCCTGTAAACGACTACGCGCCTGCTTAGCGATGGGCGT                                                >  NB501016:230:HFVJLAFXY:3:21401:4851:20322/1‑151 (MQ=60)
                                                                                                                GGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTA                                              <  NB501016:230:HFVJLAFXY:1:21106:3443:16194/140‑1 (MQ=60)
                                                                                                                   GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATG                                <  NB501016:230:HFVJLAFXY:1:21304:12560:4232/151‑1 (MQ=60)
                                                                                                                    AGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGG                               <  NB501016:230:HFVJLAFXY:2:21105:22713:5080/151‑1 (MQ=60)
                                                                                                                             AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCC                      >  NB501016:230:HFVJLAFXY:1:11101:19161:17482/1‑151 (MQ=60)
                                                                                                                             AAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTA                                              <  NB501016:230:HFVJLAFXY:1:21111:21936:14339/127‑1 (MQ=60)
                                                                                                                                      CTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATG                                >  NB501016:230:HFVJLAFXY:3:21508:4048:5381/1‑132 (MQ=60)
                                                                                                                                           CGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGC        <  NB501016:230:HFVJLAFXY:3:21604:20544:10106/151‑1 (MQ=60)
                                                                                                                                           CGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGC                                                         >  NB501016:230:HFVJLAFXY:4:11611:6608:3163/1‑102 (MQ=60)
                                                                                                                                                  ccaCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCA    >  NB501016:230:HFVJLAFXY:1:21312:14677:18680/4‑150 (MQ=60)
                                                                                                                                                  ccaCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCT                                                             >  NB501016:230:HFVJLAFXY:3:21405:12189:17174/4‑93 (MQ=60)
                                                                                                                                                   CACCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAgatcggaagagcacacgttctgaactccagtcacagcgatagatctcgtatgc  >  NB501016:230:HFVJLAFXY:4:21508:22279:15520/1‑98 (MQ=60)
                                                                                                                                                     CCGCCGTATGTCCCATCAGCAACGCCGGATCAAGGCCTATCAGGAAGTCCTGACCGCCGAACTTCGCCTGTAATCGACTACGAGCGTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAA                         <  NB501016:230:HFVJLAFXY:4:11601:3891:18923/126‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATC  >  NC_000913/2173214‑2173511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑