Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 88,227 T→C V67A (GTG→GCG)  fruR → FruR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091388,2270TC99.4% 962.1 / ‑3.5 308V67A (GTG→GCG) fruRFruR
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (149/156);  total (150/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 318 total aligned reads displayed.

AAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTG  >  NC_000913/88137‑88316
                                                                                          |                                                                                         
aaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGa                                                                                         <  1:1018304/93‑1 (MQ=255)
aaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGa                                                                                         <  2:3437103/93‑1 (MQ=255)
  gTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATc                                                                                       >  1:6327319/1‑93 (MQ=255)
   tCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcc                                                                                      >  2:1698825/1‑93 (MQ=255)
     aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcccc                                                                                    <  2:4493126/93‑1 (MQ=255)
     aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcccc                                                                                    >  2:5747156/1‑93 (MQ=255)
     aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATcccc                                                                                    >  1:3561487/1‑93 (MQ=255)
      tGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCg                                                                                   >  2:6645/1‑93 (MQ=255)
      tGGCTGTGGTGCGCGAGCACAATTACCACCCGAACGCCGGGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCg                                                                                   >  2:5563419/1‑93 (MQ=255)
       ggCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGa                                                                                  <  1:1935665/93‑1 (MQ=255)
        gCTGTGTTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGCTCCCCGAt                                                                                 >  1:2447473/1‑93 (MQ=255)
         gtgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATc                                                                                <  1:762287/92‑1 (MQ=255)
           gtggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTg                                                                              >  2:5254053/1‑93 (MQ=255)
            tggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTgg                                                                             >  2:7186920/1‑93 (MQ=255)
              gtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGgag                                                                           >  2:3904262/1‑93 (MQ=255)
                gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGaa                                                                         <  2:796628/93‑1 (MQ=255)
                gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGaa                                                                         <  2:1635098/93‑1 (MQ=255)
                gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGaa                                                                         <  1:4918783/93‑1 (MQ=255)
                  gTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAaca                                                                       >  2:271743/1‑93 (MQ=255)
                   tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAacac                                                                      >  2:2347896/1‑93 (MQ=255)
                   tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAacac                                                                      >  1:4569303/1‑93 (MQ=255)
                   tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAacac                                                                      >  1:1378465/1‑93 (MQ=255)
                    gAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACAcc                                                                     <  2:6465879/93‑1 (MQ=255)
                     aGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCa                                                                    <  1:552126/93‑1 (MQ=255)
                     aGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCa                                                                    >  2:4082176/1‑93 (MQ=255)
                      gCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGACCACCAg                                                                   >  2:1733784/1‑93 (MQ=255)
                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  2:1849204/93‑1 (MQ=255)
                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  1:5011323/93‑1 (MQ=255)
                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  1:1307157/93‑1 (MQ=255)
                       cacaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGc                                                                  <  2:7568626/93‑1 (MQ=255)
                         caATTCCCACCCGAGCGCCGTGGCAGCTGGTCTTCGTGCTGGACGCCCACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCta                                                                <  2:7122120/93‑1 (MQ=255)
                         caATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCta                                                                <  2:4829050/93‑1 (MQ=255)
                           aTTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCTCCGATCTGGAGAACACCAGCtata                                                              >  1:7489842/1‑93 (MQ=255)
                            ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAc                                                             <  2:5854583/93‑1 (MQ=255)
                            ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAc                                                             >  2:4916670/1‑93 (MQ=255)
                            ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAc                                                             >  1:3023869/1‑93 (MQ=255)
                             tACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAcc                                                            >  2:7839952/1‑93 (MQ=255)
                             tACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAcc                                                            <  1:1424802/93‑1 (MQ=255)
                             tACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAcc                                                            >  1:719350/1‑93 (MQ=255)
                                 ccccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCa                                                        <  2:6776130/92‑1 (MQ=255)
                                   ccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATc                                                      <  2:6059644/93‑1 (MQ=255)
                                      aaCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCt                                                   >  2:1781418/1‑93 (MQ=255)
                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  1:3212064/1‑93 (MQ=255)
                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  2:6891597/1‑93 (MQ=255)
                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  2:3994677/1‑93 (MQ=255)
                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  1:334293/1‑93 (MQ=255)
                                       aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                                  >  1:2551709/1‑93 (MQ=255)
                                        cGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTaa                                                 >  1:782386/1‑93 (MQ=255)
                                         gCCGTGGCAGCTGGGCTTCGTGCTGGACGCACCCGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAAc                                                <  1:5332070/93‑1 (MQ=255)
                                             ttgCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            <  1:2410701/91‑1 (MQ=255)
                                             tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            <  2:4735160/93‑1 (MQ=255)
                                             tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            >  1:3495965/1‑93 (MQ=255)
                                             tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATc                                            <  1:2306141/93‑1 (MQ=255)
                                               gCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCtt                                          <  2:857435/93‑1 (MQ=255)
                                                  gCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGaa                                       <  2:2057294/93‑1 (MQ=255)
                                                  gCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGaa                                       <  2:4367436/93‑1 (MQ=255)
                                                  gCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGaa                                       <  2:1165059/93‑1 (MQ=255)
                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  2:4387459/93‑1 (MQ=255)
                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:6001399/93‑1 (MQ=255)
                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:690220/93‑1 (MQ=255)
                                                   cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:696523/93‑1 (MQ=255)
                                                   cTGGGCCTCGTTCTGGACCCACACCTTCTATTTGGCTTGCGAACCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAc                                      <  1:5697082/93‑1 (MQ=255)
                                                    tGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACg                                     <  2:4609246/93‑1 (MQ=255)
                                                    tGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACg                                     <  1:7292577/93‑1 (MQ=255)
                                                     gggCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGc                                    >  2:615004/1‑93 (MQ=255)
                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  >  1:6840856/1‑93 (MQ=255)
                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  2:4192923/93‑1 (MQ=255)
                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  2:3310747/93‑1 (MQ=255)
                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  2:2917999/93‑1 (MQ=255)
                                                       gCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCa                                  <  1:4181153/93‑1 (MQ=255)
                                                        cTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAg                                 <  1:7701486/93‑1 (MQ=255)
                                                         ttCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAgg                                >  2:6251290/1‑93 (MQ=255)
                                                            gTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATAGCTAACTATCTTGAACGCCAGgagc                             >  1:1167378/1‑93 (MQ=255)
                                                              gCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACCCCAGCTATACCCCCATCGCTAACTATCTTGAACGCCAcgcgcgc                           >  1:5827530/1‑92 (MQ=255)
                                                              gCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCgg                           >  2:4318980/1‑93 (MQ=255)
                                                                tGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCa                         <  1:3118921/93‑1 (MQ=255)
                                                                tGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCa                         >  1:4366614/1‑93 (MQ=255)
                                                                 ggACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCaa                        <  1:908081/93‑1 (MQ=255)
                                                                  gACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCaag                       >  2:1456289/1‑92 (MQ=255)
                                                                  gACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAc                       >  1:6521999/1‑93 (MQ=255)
                                                                    cGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgg                     >  2:5600103/1‑93 (MQ=255)
                                                                     gCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACggg                    >  2:1216338/1‑93 (MQ=255)
                                                                     gCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACggg                    <  2:3602869/93‑1 (MQ=255)
                                                                     gCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACggg                    <  2:316825/93‑1 (MQ=255)
                                                                       acacGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGt                  >  2:5017897/1‑93 (MQ=255)
                                                                       acacGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGt                  <  1:668986/93‑1 (MQ=255)
                                                                        cacGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGtt                 <  1:6178363/93‑1 (MQ=255)
                                                                             tCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCaa            >  2:2241328/1‑93 (MQ=255)
                                                                             tCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCaa            >  1:5127483/1‑93 (MQ=255)
                                                                              cTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAc           >  2:2803879/1‑93 (MQ=255)
                                                                              cTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAc           <  1:856127/93‑1 (MQ=255)
                                                                               tATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAct          <  2:7732210/93‑1 (MQ=255)
                                                                               tATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAct          >  2:4414207/1‑93 (MQ=255)
                                                                                aTTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActg         >  2:6238824/1‑93 (MQ=255)
                                                                                 ttGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgc        <  1:5710858/93‑1 (MQ=255)
                                                                                  tGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgcg       >  2:5686214/1‑92 (MQ=255)
                                                                                   ggTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgctg      <  2:7387416/93‑1 (MQ=255)
                                                                                    gTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGa     <  1:262027/93‑1 (MQ=255)
                                                                                       ttGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTg  >  2:2838910/1‑93 (MQ=255)
                                                                                       ttGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTg  >  1:1984892/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTG  >  NC_000913/88137‑88316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCT  >  NC_000913/88134‑88319
                                                                                             |                                                                                            
AAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGC                                                                                              <  SRR4302543.2413647/94‑1 (MQ=60)
AAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGC                                                                                              >  SRR4302543.2997979/1‑94 (MQ=60)
AAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGC                                                                                              >  SRR4302543.5817046/1‑94 (MQ=60)
AAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCAGTGGCAGCTGGGCTTCGTGCTGGACGCACAAGTTCTATTGGTCTTGG                                                                                              >  SRR4302543.3760112/1‑94 (MQ=60)
 AAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGGCTTGCG                                                                                             >  SRR4302543.6042107/1‑94 (MQ=60)
  AAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGA                                                                                            <  SRR4302543.2530906/94‑1 (MQ=60)
   AAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGAT                                                                                           <  SRR4302543.1027313/94‑1 (MQ=60)
    AGTCATGGCTGTGGTGCGTGAGCACAATTGCCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATC                                                                                          <  SRR4302543.5345330/94‑1 (MQ=60)
    AGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATC                                                                                          >  SRR4302543.6386826/1‑94 (MQ=60)
     GTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCC                                                                                         >  SRR4302543.3269204/1‑94 (MQ=60)
      TCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCC                                                                                        >  SRR4302543.4909240/1‑94 (MQ=60)
       CATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCC                                                                                       >  SRR4302543.3593019/1‑94 (MQ=60)
       CATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCC                                                                                       >  SRR4302543.2389382/1‑94 (MQ=60)
       CATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCC                                                                                       >  SRR4302543.6328577/1‑94 (MQ=60)
       CATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCC                                                                                       >  SRR4302543.4859723/1‑94 (MQ=60)
          GGCTGTGTTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGCTCCCCGAT                                                                                    >  SRR4302543.2469541/1‑94 (MQ=60)
          GGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGAT                                                                                    >  SRR4302543.6970355/1‑94 (MQ=60)
          GGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGAT                                                                                    >  SRR4302543.111623/1‑94 (MQ=60)
          GGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGAT                                                                                    <  SRR4302543.1953066/94‑1 (MQ=60)
            GTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCT                                                                                  <  SRR4302543.769006/94‑1 (MQ=60)
            CTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCT                                                                                  <  SRR4302543.1311019/94‑1 (MQ=60)
             TGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTG                                                                                 >  SRR4302543.6674336/1‑94 (MQ=60)
             TGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTG                                                                                 >  SRR4302543.7029168/1‑94 (MQ=60)
             TGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTG                                                                                 >  SRR4302543.5431479/1‑94 (MQ=60)
              GTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGCGATCCCCGATCTGG                                                                                >  SRR4302543.7674534/1‑94 (MQ=60)
              GTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGG                                                                                <  SRR4302543.5591563/94‑1 (MQ=60)
               TGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGA                                                                               >  SRR4302543.5757002/1‑94 (MQ=60)
               TGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGA                                                                               >  SRR4302543.295730/1‑94 (MQ=60)
                GGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAG                                                                              <  SRR4302543.7121334/94‑1 (MQ=60)
                GGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAG                                                                              <  SRR4302543.6583178/94‑1 (MQ=60)
                  TGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAA                                                                            >  SRR4302543.7754564/1‑94 (MQ=60)
                   GCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAAC                                                                           <  SRR4302543.4963596/94‑1 (MQ=60)
                    CGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACA                                                                          >  SRR4302543.252337/1‑94 (MQ=60)
                     GTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACAC                                                                         >  SRR4302543.4610859/1‑94 (MQ=60)
                     GTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACAC                                                                         >  SRR4302543.1390853/1‑94 (MQ=60)
                      TGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACC                                                                        >  SRR4302543.2054838/1‑94 (MQ=60)
                       GAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCA                                                                       <  SRR4302543.624373/94‑1 (MQ=60)
                        AGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAG                                                                      <  SRR4302543.557201/94‑1 (MQ=60)
                         GCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGC                                                                     <  SRR4302543.7209112/94‑1 (MQ=60)
                          CACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCT                                                                    <  SRR4302543.5056933/94‑1 (MQ=60)
                          CACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCT                                                                    <  SRR4302543.1318768/94‑1 (MQ=60)
                          CACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCT                                                                    >  SRR4302543.3088185/1‑94 (MQ=60)
                          CACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCT                                                                    >  SRR4302543.2788970/1‑94 (MQ=60)
                          CACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGAGATCCCCGATCTGGAGAACACCAGCT                                                                    <  SRR4302543.3106740/94‑1 (MQ=60)
                           ACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTA                                                                   <  SRR4302543.4261681/94‑1 (MQ=60)
                            CAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTAT                                                                  >  SRR4302543.5905727/1‑94 (MQ=60)
                            CAATGACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATCGGTCTTGCGATCACCGATCTGGAGAACACCAGCTAT                                                                  <  SRR4302543.1387773/94‑1 (MQ=60)
                             AATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCTCCGATCTGGAGAACACCAGCTATA                                                                 >  SRR4302543.7561505/1‑94 (MQ=60)
                             AATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGGCTTGCGATCCCCGATCTGGAGAACACCAGCTATA                                                                 >  SRR4302543.1180181/1‑94 (MQ=60)
                              ATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATAC                                                                >  SRR4302543.3051199/1‑94 (MQ=60)
                               TTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACC                                                               >  SRR4302543.725851/1‑94 (MQ=60)
                               TTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACC                                                               <  SRR4302543.684055/94‑1 (MQ=60)
                                TACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCC                                                              <  SRR4302543.1437698/94‑1 (MQ=60)
                                  CCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGC                                                            <  SRR4302543.679963/94‑1 (MQ=60)
                                  CCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGC                                                            >  SRR4302543.881330/1‑94 (MQ=60)
                                   CACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCA                                                           <  SRR4302543.2463295/94‑1 (MQ=60)
                                    CCCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCAT                                                          <  SRR4302543.4271700/94‑1 (MQ=60)
                                    CCCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCAT                                                          <  SRR4302543.5016909/94‑1 (MQ=60)
                                    ACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCAT                                                          >  SRR4302543.6961300/1‑94 (MQ=60)
                                        GAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT                                                      <  SRR4302543.7699402/94‑1 (MQ=60)
                                        GAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT                                                      >  SRR4302543.1409922/1‑94 (MQ=60)
                                        GAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT                                                      <  SRR4302543.3554303/94‑1 (MQ=60)
                                         AACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTA                                                     >  SRR4302543.2575021/1‑94 (MQ=60)
                                         AACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTA                                                     >  SRR4302543.337360/1‑94 (MQ=60)
                                         AACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTA                                                     <  SRR4302543.730948/94‑1 (MQ=60)
                                         AACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTA                                                     >  SRR4302543.3241073/1‑94 (MQ=60)
                                          ACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAA                                                    >  SRR4302543.789270/1‑94 (MQ=60)
                                            GCCGTGGCAGCTGGGCTTCGTGCTGGACGCACCCGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACT                                                  <  SRR4302543.5380287/94‑1 (MQ=60)
                                             CCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTA                                                 >  SRR4302543.1041299/1‑94 (MQ=60)
                                             CCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTA                                                 >  SRR4302543.2467987/1‑94 (MQ=60)
                                               GTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATC                                               >  SRR4302543.3527242/1‑94 (MQ=60)
                                               GTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATC                                               >  SRR4302543.3527243/1‑94 (MQ=60)
                                                TTGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCT                                              <  SRR4302543.2432488/94‑1 (MQ=60)
                                                TGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCT                                              <  SRR4302543.2327146/94‑1 (MQ=60)
                                                TGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCT                                              <  SRR4302543.766513/94‑1 (MQ=60)
                                                 GGCAGCTGGGTTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTT                                             <  SRR4302543.3535372/94‑1 (MQ=60)
                                                   CAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGA                                           <  SRR4302543.344375/94‑1 (MQ=60)
                                                   CAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGA                                           >  SRR4302543.5871481/1‑94 (MQ=60)
                                                    AGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAA                                          <  SRR4302543.5068268/94‑1 (MQ=60)
                                                    AGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAA                                          >  SRR4302543.1962068/1‑94 (MQ=60)
                                                     GCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAC                                         <  SRR4302543.7928830/94‑1 (MQ=60)
                                                     GCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAC                                         <  SRR4302543.7440168/94‑1 (MQ=60)
                                                      CTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACG                                        <  SRR4302543.6056560/94‑1 (MQ=60)
                                                      CTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACG                                        <  SRR4302543.7388131/94‑1 (MQ=60)
                                                      CTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACG                                        <  SRR4302543.702901/94‑1 (MQ=60)
                                                      CTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACG                                        <  SRR4302543.696559/94‑1 (MQ=60)
                                                      CTGGGCGTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACG                                        <  SRR4302543.5749056/94‑1 (MQ=60)
                                                      CTGGGCCTCGTTCTGGACCCACACCTTCTATTTGGCTTGCGAACCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACG                                        <  SRR4302543.5749055/94‑1 (MQ=60)
                                                       TGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGC                                       <  SRR4302543.7362954/94‑1 (MQ=60)
                                                        GGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCC                                      <  SRR4302543.620697/94‑1 (MQ=60)
                                                         GGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCA                                     >  SRR4302543.6905423/1‑94 (MQ=60)
                                                          GCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAG                                    <  SRR4302543.4219042/94‑1 (MQ=60)
                                                           CTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGG                                   <  SRR4302543.7774372/94‑1 (MQ=60)
                                                           CTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGG                                   >  SRR4302543.778435/1‑94 (MQ=60)
                                                           CTTCGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGG                                   >  SRR4302543.3367268/1‑94 (MQ=60)
                                                            TTCGTGCGGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGC                                  <  SRR4302543.97812/94‑1 (MQ=60)
                                                              CGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGC                                <  SRR4302543.898331/94‑1 (MQ=60)
                                                              CGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGC                                >  SRR4302543.4105223/1‑94 (MQ=60)
                                                              CGTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATAGCTAACTATCTTGAACGCCAGGAGC                                >  SRR4302543.1177985/1‑94 (MQ=60)
                                                               GTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAAGGCCAGGCGCG                               >  SRR4302543.2910062/1‑94 (MQ=60)
                                                               GTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCG                               <  SRR4302543.1603352/94‑1 (MQ=60)
                                                               GTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCG                               <  SRR4302543.6637090/94‑1 (MQ=60)
                                                               GTGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCG                               <  SRR4302543.5103732/94‑1 (MQ=60)
                                                                TGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACCCCAGCTATACCCCCATCGCTAACTATCTTGAACGCCACGCGCGC                              >  SRR4302543.5880521/1‑94 (MQ=60)
                                                                TGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGG                              <  SRR4302543.2604494/94‑1 (MQ=60)
                                                                TGCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGG                              >  SRR4302543.5175704/1‑94 (MQ=60)
                                                                 GCTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGC                             >  SRR4302543.5495863/1‑94 (MQ=60)
                                                                  CTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCA                            >  SRR4302543.1999947/1‑94 (MQ=60)
                                                                  CTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCA                            >  SRR4302543.7544453/1‑94 (MQ=60)
                                                                  CTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCA                            >  SRR4302543.4406168/1‑94 (MQ=60)
                                                                  CTGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCA                            <  SRR4302543.3796431/94‑1 (MQ=60)
                                                                   TGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAA                           <  SRR4302543.3146978/94‑1 (MQ=60)
                                                                   TGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAA                           >  SRR4302543.6218748/1‑94 (MQ=60)
                                                                   TGGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAA                           >  SRR4302543.5795823/1‑94 (MQ=60)
                                                                    GGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAC                          <  SRR4302543.916073/94‑1 (MQ=60)
                                                                    GGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAC                          <  SRR4302543.916074/94‑1 (MQ=60)
                                                                    GGACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAC                          >  SRR4302543.6583416/1‑94 (MQ=60)
                                                                     GACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACG                         <  SRR4302543.2208802/94‑1 (MQ=60)
                                                                      ACGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGG                        >  SRR4302543.2619877/1‑94 (MQ=60)
                                                                       CGCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGG                       >  SRR4302543.5674855/1‑94 (MQ=60)
                                                                        GCACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGG                      <  SRR4302543.2353571/94‑1 (MQ=60)
                                                                         CACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGT                     <  SRR4302543.2566930/94‑1 (MQ=60)
                                                                         CACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGT                     <  SRR4302543.3548115/94‑1 (MQ=60)
                                                                         CACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGT                     <  SRR4302543.799652/94‑1 (MQ=60)
                                                                         CACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGT                     >  SRR4302543.6019631/1‑94 (MQ=60)
                                                                          ACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTT                    <  SRR4302543.675151/94‑1 (MQ=60)
                                                                          ACACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTT                    <  SRR4302543.1953950/94‑1 (MQ=60)
                                                                           CACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTA                   <  SRR4302543.142026/94‑1 (MQ=60)
                                                                           CACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTA                   <  SRR4302543.6236772/94‑1 (MQ=60)
                                                                            ACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTAT                  <  SRR4302543.6414096/94‑1 (MQ=60)
                                                                             CGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATC                 <  SRR4302543.6913735/94‑1 (MQ=60)
                                                                             CGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATC                 >  SRR4302543.7885886/1‑94 (MQ=60)
                                                                              GTTCTATTGTTCTTGCGATACCCGATCTGGAGGTCACCAGCTATACCCGCATCGCTAACTATCTTCAACGCCAGactgggccgcggagttatca                >  SRR4302543.5086156/1‑74 (MQ=52)
                                                                              GTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCA                >  SRR4302543.5086162/1‑94 (MQ=60)
                                                                              GTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCA                >  SRR4302543.1836314/1‑94 (MQ=60)
                                                                              GTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCA                >  SRR4302543.5005595/1‑94 (MQ=60)
                                                                              GTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCA                >  SRR4302543.4484914/1‑94 (MQ=60)
                                                                              GTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCA                >  SRR4302543.6838584/1‑94 (MQ=60)
                                                                               TTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAA               >  SRR4302543.5173830/1‑94 (MQ=60)
                                                                               TTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGGTATCAA               >  SRR4302543.7280154/1‑94 (MQ=60)
                                                                                 CTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACT             <  SRR4302543.863750/94‑1 (MQ=60)
                                                                                  TATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTG            <  SRR4302543.4463183/94‑1 (MQ=60)
                                                                                    TTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCT          <  SRR4302543.5762956/94‑1 (MQ=60)
                                                                                    TTGGTCTTGCGATACCCGATCTGGAGACCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCT          <  SRR4302543.5762957/94‑1 (MQ=60)
                                                                                     TGGTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTG         >  SRR4302543.3201470/1‑94 (MQ=60)
                                                                                       GTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGAT       <  SRR4302543.3196599/94‑1 (MQ=60)
                                                                                       GTCTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGAT       <  SRR4302543.264321/94‑1 (MQ=60)
                                                                                         CTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTG     >  SRR4302543.1348225/1‑94 (MQ=60)
                                                                                         CTTGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTG     >  SRR4302543.2002693/1‑94 (MQ=60)
                                                                                           TGCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCC   >  SRR4302543.380053/1‑94 (MQ=60)
                                                                                            GCGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCT  <  SRR4302543.5006325/94‑1 (MQ=60)
                                                                                             |                                                                                            
AAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCT  >  NC_000913/88134‑88319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑