Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 105,471 A→G Q56R (CAG→CGG)  ftsZ → FtsZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913105,4710AG100.0% 868.8 / NA 278Q56R (CAG→CGG) ftsZFtsZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (137/141);  total (137/141)

Only 100 of 290 total aligned reads displayed.

TTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACAGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCG  >  NC_000913/105381‑105560
                                                                                          |                                                                                         
ttGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGa                                                                                         >  1:1137711/1‑93 (MQ=255)
ttGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGa                                                                                         <  2:3620480/93‑1 (MQ=255)
ttGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGa                                                                                         <  1:2658405/93‑1 (MQ=255)
 tGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTggacggac                                                                                        <  1:3148471/93‑1 (MQ=255)
 tGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTggacggac                                                                                        <  1:2822455/93‑1 (MQ=255)
   aaCACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGa                                                                                      >  2:6578331/1‑93 (MQ=255)
     cacaTGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGAtt                                                                                    >  2:1293939/1‑93 (MQ=255)
     cacaTGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGAtt                                                                                    >  2:6792390/1‑93 (MQ=255)
       caTGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCa                                                                                  >  2:5515099/1‑93 (MQ=255)
        aTGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCaa                                                                                 >  2:1948271/1‑93 (MQ=255)
        aTGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCaa                                                                                 <  2:2541305/93‑1 (MQ=255)
            tGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCg                                                                             >  1:2477297/1‑93 (MQ=255)
             gcgcGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCgg                                                                            <  2:4978424/93‑1 (MQ=255)
              cgcgAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGt                                                                           <  1:3551232/93‑1 (MQ=255)
              cgcgAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGt                                                                           >  1:6917839/1‑93 (MQ=255)
               gcgAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTa                                                                          <  2:4683349/93‑1 (MQ=255)
               gcgAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTa                                                                          <  1:7644719/93‑1 (MQ=255)
                cgAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAg                                                                         <  2:2469281/93‑1 (MQ=255)
                cgAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAg                                                                         <  2:7393693/93‑1 (MQ=255)
                    cgcATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGt                                                                     <  1:6418253/93‑1 (MQ=255)
                     tcATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTa                                                                    <  2:408669/92‑1 (MQ=255)
                     gcATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTa                                                                    <  1:2106867/93‑1 (MQ=255)
                      cATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTAt                                                                   <  2:3907719/93‑1 (MQ=255)
                       aTTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATc                                                                  >  2:1148386/1‑93 (MQ=255)
                       aTTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATc                                                                  >  1:5784681/1‑93 (MQ=255)
                        ttGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCa                                                                 >  2:2228356/1‑93 (MQ=255)
                         tGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCAc                                                                >  1:2255070/1‑93 (MQ=255)
                          gAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCAcc                                                               <  1:1031989/93‑1 (MQ=255)
                          gAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCAcc                                                               >  1:5602939/1‑93 (MQ=255)
                          gAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCAcc                                                               >  1:6094462/1‑93 (MQ=255)
                            aGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCaa                                                             <  1:1621263/93‑1 (MQ=255)
                            aGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCaa                                                             >  2:23276/1‑93 (MQ=255)
                             ggTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCaaa                                                            >  2:3799460/1‑93 (MQ=255)
                             ggTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCaaa                                                            >  1:4849135/1‑93 (MQ=255)
                             ggTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCaaa                                                            <  1:4208628/93‑1 (MQ=255)
                             ggTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCaaa                                                            <  2:1431698/93‑1 (MQ=255)
                              gtgtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAg                                                           <  2:5960240/93‑1 (MQ=255)
                              gtgtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAg                                                           <  1:3932394/93‑1 (MQ=255)
                               tgtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAgg                                                          >  1:6497270/1‑93 (MQ=255)
                                gtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGa                                                         >  2:5051193/1‑93 (MQ=255)
                                gtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGa                                                         <  1:6883947/93‑1 (MQ=255)
                                gtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGa                                                         >  1:2338061/1‑93 (MQ=255)
                                gtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAAAAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGa                                                         <  2:3954837/93‑1 (MQ=255)
                                gtTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGGAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGGATCACCAAAAGa                                                         >  2:2930698/1‑93 (MQ=255)
                                 ttGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGAc                                                        >  2:7071930/1‑93 (MQ=255)
                                 ttGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGAc                                                        <  1:7782423/93‑1 (MQ=255)
                                 ttGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGAc                                                        >  1:521193/1‑93 (MQ=255)
                                  tGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACt                                                       <  1:6707174/93‑1 (MQ=255)
                                    aaTTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTgg                                                     <  2:6688778/93‑1 (MQ=255)
                                    aaTTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTgg                                                     >  2:7533614/1‑93 (MQ=255)
                                    aaTTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTgg                                                     >  1:5757736/1‑93 (MQ=255)
                                    aaTTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTgg                                                     <  2:7611080/93‑1 (MQ=255)
                                        cttcGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgc                                                 >  1:858654/1‑93 (MQ=255)
                                          tcGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctg                                               <  2:5689215/93‑1 (MQ=255)
                                           cgcgGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctgg                                              <  1:3302317/93‑1 (MQ=255)
                                           cgcgGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctgg                                              <  1:527682/93‑1 (MQ=255)
                                             cgGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctggcg                                            <  1:6223238/93‑1 (MQ=255)
                                               gTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctggcgct                                          >  1:4970272/1‑93 (MQ=255)
                                               gTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctggcgct                                          >  2:4169887/1‑93 (MQ=255)
                                               gTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctggcgct                                          >  2:6972802/1‑93 (MQ=255)
                                               gTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGggcgctggcgct                                          >  2:452320/1‑93 (MQ=255)
                                                tAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTa                                         >  1:3017547/1‑93 (MQ=255)
                                                 aaaTACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTaa                                        <  2:4866268/93‑1 (MQ=255)
                                                 aaaTACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTaa                                        <  1:1900/93‑1 (MQ=255)
                                                  aaTACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAAt                                       >  2:3318135/1‑93 (MQ=255)
                                                    tACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATcc                                     >  1:4528958/1‑93 (MQ=255)
                                                     aCCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCa                                    <  2:2615836/93‑1 (MQ=255)
                                                      ccGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAg                                   <  1:2075433/93‑1 (MQ=255)
                                                      ccGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAg                                   >  1:1875538/1‑93 (MQ=255)
                                                       cGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGa                                  <  2:2597007/93‑1 (MQ=255)
                                                        gATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGaa                                 <  1:7841990/93‑1 (MQ=255)
                                                        gATGCACAAGCGCTGCGTAAAACAGCGGTTGCACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGaa                                 <  1:4001307/93‑1 (MQ=255)
                                                          tGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCCAAGGACTGGGAGCTGGCGCTAATGCAGAAGt                               >  2:554912/1‑93 (MQ=255)
                                                           gCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGtt                              <  2:5623186/93‑1 (MQ=255)
                                                           gCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGtt                              <  2:6335386/93‑1 (MQ=255)
                                                            cacaAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTg                             <  2:6459775/93‑1 (MQ=255)
                                                             acaAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTgg                            >  2:5160252/1‑93 (MQ=255)
                                                              caAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTGTCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGc                           >  1:6792690/1‑93 (MQ=255)
                                                              caAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGc                           >  2:4374686/1‑93 (MQ=255)
                                                                aGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCg                         >  2:390913/1‑93 (MQ=255)
                                                                   gcTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCaa                      >  2:1100045/1‑93 (MQ=255)
                                                                   gcTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCaa                      <  1:3387232/93‑1 (MQ=255)
                                                                   gcTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCaa                      <  2:2692661/93‑1 (MQ=255)
                                                                     tGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAAtc                    >  1:4088928/1‑92 (MQ=255)
                                                                     tGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATg                    >  1:673332/1‑93 (MQ=255)
                                                                     tGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATg                    <  1:7255076/93‑1 (MQ=255)
                                                                       cGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCg                  <  2:3550183/93‑1 (MQ=255)
                                                                        gTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCgg                 >  2:7898608/1‑93 (MQ=255)
                                                                        gTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCgg                 >  2:5813453/1‑93 (MQ=255)
                                                                         tAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGc                >  1:3773459/1‑93 (MQ=255)
                                                                         tAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGc                <  1:4029432/93‑1 (MQ=255)
                                                                          aaaaCAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCt               <  2:2954253/93‑1 (MQ=255)
                                                                           aaaCAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCtg              >  1:1780708/1‑93 (MQ=255)
                                                                            aaCAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCtga             <  1:1372065/93‑1 (MQ=255)
                                                                            aaCAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCtga             <  1:830128/93‑1 (MQ=255)
                                                                               aGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCtgatga          >  2:2838138/1‑93 (MQ=255)
                                                                                gCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAg         <  1:3614800/93‑1 (MQ=255)
                                                                                   gTTAGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGAt      <  2:7574872/93‑1 (MQ=255)
                                                                                     tGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATcg    >  1:1815492/1‑93 (MQ=255)
                                                                                     tGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATcg    <  1:1293854/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACAGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCG  >  NC_000913/105381‑105560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACAGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCGATG  >  NC_000913/105378‑105563
                                                                                             |                                                                                            
CTGTTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACG                                                                                              <  SRR4302543.5343213/94‑1 (MQ=60)
  GTTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGA                                                                                            >  SRR4302543.1148057/1‑94 (MQ=60)
   TTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGAC                                                                                           <  SRR4302543.1693/94‑1 (MQ=60)
   TTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGAC                                                                                           <  SRR4302543.2682505/94‑1 (MQ=60)
    TGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACG                                                                                          <  SRR4302543.2848278/94‑1 (MQ=60)
    TGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACG                                                                                          <  SRR4302543.3176789/94‑1 (MQ=60)
    TGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACG                                                                                          <  SRR4302543.3507021/94‑1 (MQ=60)
        CACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTC                                                                                      >  SRR4302543.4078781/1‑94 (MQ=60)
          CATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAA                                                                                    >  SRR4302543.1317604/1‑94 (MQ=60)
             GGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATC                                                                                 <  SRR4302543.1885903/94‑1 (MQ=60)
              GTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCG                                                                                >  SRR4302543.2499702/1‑94 (MQ=60)
              GTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCG                                                                                >  SRR4302543.5390022/1‑94 (MQ=60)
              GTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCG                                                                                <  SRR4302543.2068411/94‑1 (MQ=60)
              GTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCG                                                                                <  SRR4302543.190459/94‑1 (MQ=60)
              GTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCG                                                                                <  SRR4302543.4992111/94‑1 (MQ=60)
               TGCGCGAGCCCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGG                                                                               <  SRR4302543.5789078/94‑1 (MQ=60)
                GCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATCCCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGT                                                                              <  SRR4302543.4137702/94‑1 (MQ=60)
                GCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGT                                                                              >  SRR4302543.6982874/1‑94 (MQ=60)
                GCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGT                                                                              >  SRR4302543.6548485/1‑94 (MQ=60)
                 CGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCNGTNNNACNGACGATTNNANNNGNNA                                                                             >  SRR4302543.123376/1‑94 (MQ=60)
                 CGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTA                                                                             <  SRR4302543.1809931/94‑1 (MQ=60)
                 CGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTA                                                                             <  SRR4302543.186010/94‑1 (MQ=60)
                 CGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTA                                                                             <  SRR4302543.3582731/94‑1 (MQ=60)
                 CGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTA                                                                             >  SRR4302543.3986999/1‑94 (MQ=60)
                  GCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAG                                                                            <  SRR4302543.4263903/94‑1 (MQ=60)
                  GCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAG                                                                            <  SRR4302543.7717217/94‑1 (MQ=60)
                   CGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGC                                                                           >  SRR4302543.4080189/1‑94 (MQ=60)
                   CGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGC                                                                           <  SRR4302543.6694053/94‑1 (MQ=60)
                     AGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGG                                                                         >  SRR4302543.2115823/1‑94 (MQ=60)
                       CGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTA                                                                       >  SRR4302543.1960761/1‑94 (MQ=60)
                       CGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTA                                                                       <  SRR4302543.5491449/94‑1 (MQ=60)
                       CGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTA                                                                       >  SRR4302543.2124157/1‑94 (MQ=60)
                       CGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTA                                                                       <  SRR4302543.6478709/94‑1 (MQ=60)
                        GCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTAT                                                                      <  SRR4302543.2125722/94‑1 (MQ=60)
                        GCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTAT                                                                      <  SRR4302543.4040570/94‑1 (MQ=60)
                        GCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTAT                                                                      >  SRR4302543.683426/1‑94 (MQ=60)
                        GCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTAT                                                                      <  SRR4302543.2411028/94‑1 (MQ=60)
                         CATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATC                                                                     >  SRR4302543.5814580/1‑94 (MQ=60)
                         CATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATC                                                                     >  SRR4302543.5837318/1‑94 (MQ=60)
                         CATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATC                                                                     <  SRR4302543.3338609/94‑1 (MQ=60)
                          ATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCA                                                                    >  SRR4302543.7911898/1‑94 (MQ=60)
                          ATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCA                                                                    >  SRR4302543.5811866/1‑94 (MQ=60)
                           TTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCAC                                                                   >  SRR4302543.2275585/1‑94 (MQ=60)
                            TGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACC                                                                  >  SRR4302543.6152219/1‑94 (MQ=60)
                            TGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACC                                                                  >  SRR4302543.7141060/1‑94 (MQ=60)
                            TGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACC                                                                  >  SRR4302543.5653992/1‑94 (MQ=60)
                             GAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCA                                                                 <  SRR4302543.4474667/94‑1 (MQ=60)
                             GAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCA                                                                 <  SRR4302543.4746183/94‑1 (MQ=60)
                             GAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCA                                                                 <  SRR4302543.1041153/94‑1 (MQ=60)
                             GAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCA                                                                 >  SRR4302543.2950187/1‑94 (MQ=60)
                               AGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAA                                                               >  SRR4302543.4893353/1‑94 (MQ=60)
                               AGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAA                                                               <  SRR4302543.1635711/94‑1 (MQ=60)
                                GGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAG                                                              <  SRR4302543.4246787/94‑1 (MQ=60)
                                 GTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGG                                                             <  SRR4302543.3967126/94‑1 (MQ=60)
                                 GTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGG                                                             >  SRR4302543.612891/1‑94 (MQ=60)
                                 GTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGG                                                             >  SRR4302543.2675917/1‑94 (MQ=60)
                                 GTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGG                                                             >  SRR4302543.6558478/1‑94 (MQ=60)
                                  TGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGA                                                            >  SRR4302543.2359277/1‑94 (MQ=60)
                                   GTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGAC                                                           >  SRR4302543.7962057/1‑94 (MQ=60)
                                   GTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGAC                                                           >  SRR4302543.526030/1‑94 (MQ=60)
                                   GTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGAC                                                           >  SRR4302543.215772/1‑94 (MQ=60)
                                   GTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGAC                                                           <  SRR4302543.6948708/94‑1 (MQ=60)
                                    TTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACT                                                          <  SRR4302543.4162637/94‑1 (MQ=60)
                                    TTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACT                                                          >  SRR4302543.6965031/1‑94 (MQ=60)
                                    TTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACT                                                          <  SRR4302543.7855799/94‑1 (MQ=60)
                                    TTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACT                                                          <  SRR4302543.6165191/94‑1 (MQ=60)
                                     TGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTG                                                         >  SRR4302543.1198407/1‑94 (MQ=60)
                                     TGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTG                                                         <  SRR4302543.4899791/94‑1 (MQ=60)
                                     TGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTG                                                         <  SRR4302543.6770499/94‑1 (MQ=60)
                                     TGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTG                                                         <  SRR4302543.859779/94‑1 (MQ=60)
                                     TGAATTCTGCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTG                                                         <  SRR4302543.3910966/94‑1 (MQ=60)
                                      GAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGG                                                        >  SRR4302543.2920494/1‑94 (MQ=60)
                                      GAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGG                                                        <  SRR4302543.3796010/94‑1 (MQ=60)
                                      GAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGG                                                        <  SRR4302543.3796008/94‑1 (MQ=60)
                                      GAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGG                                                        <  SRR4302543.2314284/94‑1 (MQ=60)
                                      GAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGG                                                        >  SRR4302543.5810199/1‑94 (MQ=60)
                                       AATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGG                                                       <  SRR4302543.1003765/94‑1 (MQ=60)
                                       AATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGG                                                       <  SRR4302543.1462619/94‑1 (MQ=60)
                                         TTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCG                                                     >  SRR4302543.4621921/1‑94 (MQ=60)
                                         TTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCG                                                     <  SRR4302543.244889/94‑1 (MQ=60)
                                          TCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGC                                                    >  SRR4302543.866308/1‑94 (MQ=60)
                                          TCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGC                                                    >  SRR4302543.2730437/1‑94 (MQ=60)
                                             TCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGG                                                 <  SRR4302543.7819483/94‑1 (MQ=60)
                                              CGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGC                                                <  SRR4302543.3332115/94‑1 (MQ=60)
                                              CGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGC                                                <  SRR4302543.532564/94‑1 (MQ=60)
                                              CGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGC                                                >  SRR4302543.2353298/1‑94 (MQ=60)
                                               GCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCG                                               >  SRR4302543.6950266/1‑94 (MQ=60)
                                               GCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCG                                               <  SRR4302543.3365039/94‑1 (MQ=60)
                                                CGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGC                                              <  SRR4302543.6281983/94‑1 (MQ=60)
                                                CGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGC                                              >  SRR4302543.3639796/1‑94 (MQ=60)
                                                 GGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCT                                             >  SRR4302543.5015505/1‑94 (MQ=60)
                                                 GGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCT                                             >  SRR4302543.3979074/1‑94 (MQ=60)
                                                 GGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCT                                             <  SRR4302543.6768386/94‑1 (MQ=60)
                                                  GTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTA                                            >  SRR4302543.3044836/1‑94 (MQ=60)
                                                    AAATACCGATTCACAAGCGCTGCGTAAAACAGCGGCTGGACAaacaactcaaatgagtcgaggcatcaccaaagacctctgcactgccccgctc                                          >  SRR4302543.5706187/1‑42 (MQ=60)
                                                    AAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAAT                                          <  SRR4302543.1920/94‑1 (MQ=60)
                                                    AAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAAT                                          >  SRR4302543.1100984/1‑94 (MQ=60)
                                                    AAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAAT                                          <  SRR4302543.4044169/94‑1 (MQ=60)
                                                     AATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATC                                         >  SRR4302543.1184965/1‑94 (MQ=60)
                                                      ATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCC                                        >  SRR4302543.7150055/1‑94 (MQ=60)
                                                      ATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCC                                        >  SRR4302543.4570178/1‑94 (MQ=60)
                                                      ATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCC                                        >  SRR4302543.2824132/1‑94 (MQ=60)
                                                      ATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCA                                        >  SRR4302543.6774560/1‑94 (MQ=60)
                                                       TACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCA                                       <  SRR4302543.2512101/94‑1 (MQ=60)
                                                        ACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAG                                      >  SRR4302543.3285970/1‑94 (MQ=60)
                                                        ACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAG                                      >  SRR4302543.1892389/1‑94 (MQ=60)
                                                        ACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAG                                      >  SRR4302543.1552799/1‑94 (MQ=60)
                                                         CCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGGGCTGGCGCTAATCCAGA                                     >  SRR4302543.7949633/1‑94 (MQ=60)
                                                         CCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGA                                     <  SRR4302543.2093971/94‑1 (MQ=60)
                                                         CCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGA                                     <  SRR4302543.5719391/94‑1 (MQ=60)
                                                           GATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAG                                   <  SRR4302543.6373543/94‑1 (MQ=60)
                                                           GATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAG                                   <  SRR4302543.7915913/94‑1 (MQ=60)
                                                           GATGCACAAGCGCTGCGTAAAACAGCGGTTGCACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAG                                   <  SRR4302543.4037001/94‑1 (MQ=60)
                                                            ATGCACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGT                                  >  SRR4302543.316740/1‑94 (MQ=60)
                                                               CACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCCAATCGGTAGCGGTATCACCAAAGGACTGGGCGCAGGCGCTAATCCAGAAGTcgc                               >  SRR4302543.3481786/1‑91 (MQ=60)
                                                               CACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGG                               >  SRR4302543.5081076/1‑94 (MQ=60)
                                                               CACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGG                               <  SRR4302543.3313870/94‑1 (MQ=60)
                                                                ACAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTGTCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGC                              >  SRR4302543.6856835/1‑94 (MQ=60)
                                                                 CAAGCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCC                             <  SRR4302543.1311684/94‑1 (MQ=60)
                                                                    GCGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCA                          <  SRR4302543.1914371/94‑1 (MQ=60)
                                                                     CGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAA                         >  SRR4302543.5283173/1‑94 (MQ=60)
                                                                     CGCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAA                         >  SRR4302543.7817596/1‑94 (MQ=60)
                                                                      GCTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAAT                        <  SRR4302543.3417601/94‑1 (MQ=60)
                                                                       CTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATG                       >  SRR4302543.6825046/1‑94 (MQ=60)
                                                                       CTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATG                       >  SRR4302543.679528/1‑94 (MQ=60)
                                                                       CTGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATC                       >  SRR4302543.4125601/1‑94 (MQ=60)
                                                                        TGCGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGC                      <  SRR4302543.7325216/94‑1 (MQ=60)
                                                                          CGTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGG                    >  SRR4302543.3121737/1‑94 (MQ=60)
                                                                           GTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGC                   >  SRR4302543.3806686/1‑94 (MQ=60)
                                                                           GTAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCACAATGCGGC                   >  SRR4302543.6152697/1‑94 (MQ=60)
                                                                           GTAAAACAGCGGTTGGACGAACGCTTCAAATCGGGAGAGGTATCACCAAAGGACTGGGCGCTTGCGCTAAGCCAGTAGTTGGCCGCAATGAGGC                   >  SRR4302543.6152698/1‑94 (MQ=55)
                                                                            TAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCT                  <  SRR4302543.4065447/94‑1 (MQ=60)
                                                                            TAAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCT                  >  SRR4302543.3877774/1‑94 (MQ=60)
                                                                             AAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTG                 >  SRR4302543.1796628/1‑94 (MQ=60)
                                                                             AAAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTG                 >  SRR4302543.1795040/1‑94 (MQ=60)
                                                                              AAACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGA                >  SRR4302543.5260917/1‑94 (MQ=60)
                                                                               AACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGAT               >  SRR4302543.1449746/1‑94 (MQ=60)
                                                                               AACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGAT               <  SRR4302543.837445/94‑1 (MQ=60)
                                                                               AACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGAT               <  SRR4302543.837444/94‑1 (MQ=60)
                                                                               AACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGAT               <  SRR4302543.1384393/94‑1 (MQ=60)
                                                                                ACAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATG              >  SRR4302543.1798820/1‑94 (MQ=60)
                                                                                 CAGCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGA             >  SRR4302543.73450/1‑94 (MQ=60)
                                                                                   GCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGG           <  SRR4302543.6523185/94‑1 (MQ=60)
                                                                                   GCGGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGG           <  SRR4302543.3646671/94‑1 (MQ=60)
                                                                                     GGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGAT         <  SRR4302543.6892686/94‑1 (MQ=60)
                                                                                     GGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGAT         >  SRR4302543.6348403/1‑94 (MQ=60)
                                                                                     GGTTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGAT         <  SRR4302543.6892685/94‑1 (MQ=60)
                                                                                       TTGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCG       >  SRR4302543.1831778/1‑94 (MQ=60)
                                                                                        TGGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGC      <  SRR4302543.1305372/94‑1 (MQ=60)
                                                                                         GGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCG     >  SRR4302543.789496/1‑94 (MQ=60)
                                                                                         GGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCG     <  SRR4302543.6076978/94‑1 (MQ=60)
                                                                                         GGACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCG     >  SRR4302543.7359091/1‑94 (MQ=60)
                                                                                           ACGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCGAT   <  SRR4302543.498179/94‑1 (MQ=60)
                                                                                            CGGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCtctg  >  SRR4302543.102027/1‑90 (MQ=60)
                                                                                             |                                                                                            
CTGTTGAACACATGGTGCGCGAGCGCATTGAAGGTGTTGAATTCTTCGCGGTAAATACCGATGCACAAGCGCTGCGTAAAACAGCGGTTGGACAGACGATTCAAATCGGTAGCGGTATCACCAAAGGACTGGGCGCTGGCGCTAATCCAGAAGTTGGCCGCAATGCGGCTGATGAGGATCGCGATG  >  NC_000913/105378‑105563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑