Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,890,766 T→C N165S (AAC→AGC)  b1808 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,890,7660TC99.6% 760.3 / ‑5.0 243N165S (AAC→AGC) b1808hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (132/110);  total (132/111)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 255 total aligned reads displayed.

ACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGTTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAG  >  NC_000913/1890676‑1890855
                                                                                          |                                                                                         
accaccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGCCAGTTGTCGCTg                                                                                         >  2:6656324/1‑93 (MQ=255)
accaccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTg                                                                                         >  1:2969662/1‑93 (MQ=255)
  caccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGt                                                                                       <  2:2857268/93‑1 (MQ=255)
  caccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGt                                                                                       <  2:6946173/93‑1 (MQ=255)
   accacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTg                                                                                      >  2:3491104/1‑93 (MQ=255)
   accacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTg                                                                                      >  1:7730580/1‑93 (MQ=255)
    ccacATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGc                                                                                     >  2:4922927/1‑93 (MQ=255)
      ccaTCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTg                                                                                   <  1:7788341/92‑1 (MQ=255)
      acaTCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTg                                                                                   <  2:6373492/93‑1 (MQ=255)
       caTCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTgg                                                                                  <  2:7390940/93‑1 (MQ=255)
       caTCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTgg                                                                                  <  1:5582116/93‑1 (MQ=255)
         tCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGctggtgctggtg                                                                                >  2:1521008/1‑93 (MQ=255)
           ggCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGAc                                                                              >  2:3182253/1‑93 (MQ=255)
            gCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGAcc                                                                             <  2:7397871/93‑1 (MQ=255)
              gTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAg                                                                           <  1:5460551/93‑1 (MQ=255)
              gTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAg                                                                           <  2:274403/93‑1 (MQ=255)
               tCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGc                                                                          <  1:2016485/93‑1 (MQ=255)
               tCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGc                                                                          >  1:2493016/1‑93 (MQ=255)
               tCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGc                                                                          >  1:4266747/1‑93 (MQ=255)
               tCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGc                                                                          >  1:3268166/1‑93 (MQ=255)
                ccATCGCTTTTTTACGTGCTTTGGCCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGCTGCTGGTGACCAGCg                                                                         >  1:4480580/1‑93 (MQ=255)
                 cATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCgg                                                                        >  2:2038508/1‑93 (MQ=255)
                  aTCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGc                                                                       >  1:4023200/1‑93 (MQ=255)
                   tCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGcc                                                                      >  2:1709983/1‑93 (MQ=255)
                     gCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCAc                                                                    >  1:2713265/1‑93 (MQ=255)
                      cTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACg                                                                   >  1:7211318/1‑93 (MQ=255)
                       ttttttACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGc                                                                  >  2:1180240/1‑93 (MQ=255)
                          tttACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCtg                                                               >  2:5035298/1‑93 (MQ=255)
                           ttACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCtgt                                                              >  1:4676745/1‑93 (MQ=255)
                              cGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGaa                                                           <  1:2334515/93‑1 (MQ=255)
                               gTGCTTTGACCACAAAGCAATCTTTATCCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAAt                                                          <  1:4973113/93‑1 (MQ=255)
                                  cTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCtt                                                       >  2:5992412/1‑93 (MQ=255)
                                   tttGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTc                                                      <  1:3868192/93‑1 (MQ=255)
                                   tttGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTc                                                      <  1:3222074/93‑1 (MQ=255)
                                    ttGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTcc                                                     <  1:6450193/93‑1 (MQ=255)
                                        ccACAAAGCAATCTTTATTCATCGGGCAGTCGCTGCCAAGATAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCa                                                 <  2:6853652/93‑1 (MQ=255)
                                         cacaAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCAc                                                >  1:1725625/1‑93 (MQ=255)
                                          acaAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCAcc                                               >  1:5223380/1‑92 (MQ=255)
                                          acaAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACg                                               >  2:5393527/1‑93 (MQ=255)
                                          acaAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACg                                               >  2:1639832/1‑93 (MQ=255)
                                          acaAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACg                                               >  1:1370820/1‑93 (MQ=255)
                                           caAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGc                                              >  2:1865593/1‑93 (MQ=255)
                                            aaaGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCt                                             <  2:2206016/93‑1 (MQ=255)
                                             aaGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTg                                            >  1:1793591/1‑93 (MQ=255)
                                             aaGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTg                                            >  1:5937480/1‑93 (MQ=255)
                                              aGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGa                                           <  2:6408249/93‑1 (MQ=255)
                                              aGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGa                                           >  2:2385083/1‑93 (MQ=255)
                                              aGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGa                                           <  1:4065403/93‑1 (MQ=255)
                                               gCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGAc                                          <  2:2618806/93‑1 (MQ=255)
                                                 aaTCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGc                                        >  1:3651661/1‑93 (MQ=255)
                                                  aTCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCa                                       >  1:4319021/1‑93 (MQ=255)
                                                   tCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAg                                      >  1:6561491/1‑93 (MQ=255)
                                                    ttttATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAgg                                     <  1:1482073/92‑1 (MQ=255)
                                                     tttATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAggg                                    >  1:6796797/1‑92 (MQ=255)
                                                     tttATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGt                                    <  1:6730845/93‑1 (MQ=255)
                                                       tataCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACTCCTGTGAATCTTCCGCCACGCTGACGCAGGTGc                                  <  2:4364223/93‑1 (MQ=255)
                                                       tataCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGc                                  <  1:6074702/93‑1 (MQ=255)
                                                       tataCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGc                                  <  2:4739479/93‑1 (MQ=255)
                                                       tataCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGACACGCCTGTGAATCTTCCGCCACGCTGACGCGGgcgc                                  >  2:447097/1‑93 (MQ=255)
                                                          aCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGa                               <  2:5722151/93‑1 (MQ=255)
                                                           cATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGat                              <  2:5312211/93‑1 (MQ=255)
                                                            aTCGGGCAGTCGCTGCCAAGACAGTTGTCGTTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGata                             <  2:7601400/93‑1 (MQ=255)
                                                            aTCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGata                             >  1:5820911/1‑93 (MQ=255)
                                                             tCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGatat                            >  1:7486836/1‑93 (MQ=255)
                                                             tCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGatat                            >  1:5046702/1‑93 (MQ=255)
                                                             tCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGatat                            >  1:3993937/1‑93 (MQ=255)
                                                               gggCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCa                          >  1:3769415/1‑93 (MQ=255)
                                                               gggCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCa                          >  1:159243/1‑93 (MQ=255)
                                                                 gCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCAcc                        <  1:816982/93‑1 (MQ=255)
                                                                  cAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGATATCACCa                       <  1:7374796/93‑1 (MQ=255)
                                                                  cAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCa                       <  2:6295319/93‑1 (MQ=255)
                                                                   aGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCAt                      <  1:6937019/93‑1 (MQ=255)
                                                                    gTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATc                     >  1:892483/1‑93 (MQ=255)
                                                                    gTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATc                     >  2:2239500/1‑93 (MQ=255)
                                                                    gTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATc                     <  1:3400078/93‑1 (MQ=255)
                                                                     tCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCg                    >  2:5073062/1‑93 (MQ=255)
                                                                     tCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCg                    <  1:1257520/93‑1 (MQ=255)
                                                                     tCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGGGCTGATATCACCATCg                    >  1:310208/1‑93 (MQ=255)
                                                                      cGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGa                   <  1:4432999/93‑1 (MQ=255)
                                                                       gCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGAc                  <  2:4572502/93‑1 (MQ=255)
                                                                       gCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGAc                  >  2:7908461/1‑93 (MQ=255)
                                                                        cTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACt                 >  2:7088606/1‑93 (MQ=255)
                                                                         tGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTg                >  1:7193660/1‑93 (MQ=255)
                                                                          gagaaGACAGTGGGCGCTGGTGCTGGGAACCAGCGGCCACGCCTGTGAATCTTCCGCACCGCTGACGCAGGCGCTGATATCACCATCGACTGt               <  1:1408230/90‑1 (MQ=255)
                                                                          gCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGt               >  1:7514617/1‑93 (MQ=255)
                                                                          gCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGt               <  1:6220794/93‑1 (MQ=255)
                                                                          gCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGt               <  2:1302788/93‑1 (MQ=255)
                                                                          gCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGt               <  1:1987274/93‑1 (MQ=255)
                                                                              aGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGa           <  2:3711553/93‑1 (MQ=255)
                                                                               gACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGAt          <  1:217508/93‑1 (MQ=255)
                                                                                aCAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGAtt         >  2:5631408/1‑93 (MQ=255)
                                                                                aCAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGAtt         >  2:3436404/1‑93 (MQ=255)
                                                                                 cAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTa        <  1:454511/93‑1 (MQ=255)
                                                                                 cAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTa        <  2:6615115/93‑1 (MQ=255)
                                                                                  aGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAg       >  1:547984/1‑93 (MQ=255)
                                                                                   gTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGa      <  1:119812/93‑1 (MQ=255)
                                                                                    ttGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAc     <  2:812578/93‑1 (MQ=255)
                                                                                    ttGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAc     >  1:353308/1‑93 (MQ=255)
                                                                                    ttGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAc     >  1:6680771/1‑93 (MQ=255)
                                                                                      gTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCa   <  2:3842290/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGTTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAG  >  NC_000913/1890676‑1890855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGTTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGC  >  NC_000913/1890673‑1890859
                                                                                             |                                                                                             
ACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGC                                                                                               >  SRR4302543.4580617/1‑94 (MQ=60)
 CCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCT                                                                                              <  SRR4302543.2370656/94‑1 (MQ=60)
 CCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCT                                                                                              <  SRR4302543.6877100/94‑1 (MQ=60)
 ACACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCT                                                                                              <  SRR4302543.6086147/94‑1 (MQ=60)
  CACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCGCAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTG                                                                                             <  SRR4302543.7885850/94‑1 (MQ=60)
  CACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTG                                                                                             >  SRR4302543.2996631/1‑94 (MQ=60)
  CACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTG                                                                                             <  SRR4302543.5798823/94‑1 (MQ=60)
   ACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGG                                                                                            >  SRR4302543.7838444/1‑94 (MQ=60)
    CCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGT                                                                                           <  SRR4302543.2380388/94‑1 (MQ=60)
     CACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTG                                                                                          >  SRR4302543.7803642/1‑94 (MQ=60)
         CCATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGG                                                                                      <  SRR4302543.7861757/94‑1 (MQ=60)
         ACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGG                                                                                      >  SRR4302543.2005968/1‑94 (MQ=60)
          CATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGT                                                                                     <  SRR4302543.5632942/94‑1 (MQ=60)
           ATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTG                                                                                    <  SRR4302543.5974449/94‑1 (MQ=60)
            TCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGA                                                                                   >  SRR4302543.603855/1‑94 (MQ=60)
              GGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACC                                                                                 <  SRR4302543.5339172/94‑1 (MQ=60)
                CGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAG                                                                               <  SRR4302543.5774771/94‑1 (MQ=60)
                 GTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGC                                                                              >  SRR4302543.4305505/1‑94 (MQ=60)
                 GTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGC                                                                              <  SRR4302543.5510043/94‑1 (MQ=60)
                 GTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGC                                                                              >  SRR4302543.2515625/1‑94 (MQ=60)
                 GTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGC                                                                              >  SRR4302543.3297696/1‑94 (MQ=60)
                  TCCATCGCTTTTTTACGTGCTTTGGCCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGCTGCTGGTGACCAGCG                                                                             >  SRR4302543.4521266/1‑94 (MQ=60)
                  TCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCG                                                                             >  SRR4302543.4521268/1‑94 (MQ=60)
                  TCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCG                                                                             <  SRR4302543.4554186/94‑1 (MQ=60)
                  TCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCG                                                                             <  SRR4302543.2034606/94‑1 (MQ=60)
                    CATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGC                                                                           >  SRR4302543.4059142/1‑94 (MQ=60)
                      TCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCA                                                                         <  SRR4302543.5572945/94‑1 (MQ=60)
                       CGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCAC                                                                        <  SRR4302543.6397952/94‑1 (MQ=60)
                       CGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCAC                                                                        >  SRR4302543.2737921/1‑94 (MQ=60)
                        GCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACG                                                                       >  SRR4302543.7281224/1‑94 (MQ=60)
                        GCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACG                                                                       >  SRR4302543.7629677/1‑94 (MQ=60)
                        GCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACG                                                                       >  SRR4302543.1432361/1‑94 (MQ=60)
                         CTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGC                                                                      >  SRR4302543.4153080/1‑94 (MQ=60)
                         CTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGC                                                                      >  SRR4302543.5299451/1‑94 (MQ=60)
                          TTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCC                                                                     <  SRR4302543.2589881/94‑1 (MQ=60)
                           TTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCT                                                                    >  SRR4302543.4595065/1‑94 (MQ=60)
                           TTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCG                                                                    >  SRR4302543.4595271/1‑94 (MQ=60)
                            TTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTG                                                                   >  SRR4302543.6664445/1‑94 (MQ=60)
                             TTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGT                                                                  >  SRR4302543.4719382/1‑94 (MQ=60)
                             TTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGT                                                                  >  SRR4302543.7975791/1‑94 (MQ=60)
                               TACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGA                                                                <  SRR4302543.6828716/94‑1 (MQ=60)
                               TACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGA                                                                >  SRR4302543.6131548/1‑94 (MQ=60)
                                 CGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAAT                                                              <  SRR4302543.2355703/94‑1 (MQ=60)
                                  GTGCTTTGACCACAAAGCAATCTTTATCCATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATC                                                             <  SRR4302543.5018382/94‑1 (MQ=60)
                                  GTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATC                                                             <  SRR4302543.7370470/94‑1 (MQ=60)
                                   TGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCT                                                            <  SRR4302543.676381/94‑1 (MQ=60)
                                   TGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCT                                                            >  SRR4302543.7273283/1‑94 (MQ=60)
                                     CTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTC                                                          >  SRR4302543.1678243/1‑94 (MQ=60)
                                      TTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCC                                                         <  SRR4302543.3251194/94‑1 (MQ=60)
                                      TTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCC                                                         <  SRR4302543.3212396/94‑1 (MQ=60)
                                      TTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCC                                                         <  SRR4302543.7475260/94‑1 (MQ=60)
                                      TTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCC                                                         <  SRR4302543.1402570/94‑1 (MQ=60)
                                      TTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCC                                                         <  SRR4302543.3902354/94‑1 (MQ=60)
                                       TTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCG                                                        <  SRR4302543.6510976/94‑1 (MQ=60)
                                       TTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCG                                                        >  SRR4302543.2063474/1‑94 (MQ=60)
                                        TGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGC                                                       >  SRR4302543.6170627/1‑94 (MQ=60)
                                         GACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCC                                                      >  SRR4302543.6834298/1‑94 (MQ=60)
                                           CCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCAC                                                    >  SRR4302543.7754299/1‑94 (MQ=60)
                                           CCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCAC                                                    >  SRR4302543.6733003/1‑94 (MQ=60)
                                           CCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCAC                                                    <  SRR4302543.5589993/94‑1 (MQ=60)
                                           CCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCAC                                                    <  SRR4302543.4339906/94‑1 (MQ=60)
                                           CCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCAC                                                    >  SRR4302543.1741007/1‑94 (MQ=60)
                                           CCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGCGTATCTTCCGCCAC                                                    >  SRR4302543.2906301/1‑94 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACG                                                   <  SRR4302543.2237887/94‑1 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACG                                                   >  SRR4302543.5510406/1‑94 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACG                                                   >  SRR4302543.3257586/1‑94 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACG                                                   <  SRR4302543.4468730/94‑1 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACG                                                   >  SRR4302543.3617693/1‑94 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACG                                                   >  SRR4302543.1383131/1‑94 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACC                                                   >  SRR4302543.5270410/1‑94 (MQ=60)
                                            CACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGGTGTCGCTGGTGCTAGCGACCAGCCGCCACGCCTGTGAATCTTCCGCCCCG                                                   >  SRR4302543.3257584/1‑94 (MQ=60)
                                              CAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCT                                                 >  SRR4302543.5066836/1‑94 (MQ=60)
                                              CAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCT                                                 >  SRR4302543.4466913/1‑94 (MQ=60)
                                               AAAGCAATCTTTATACATCGGGCATTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTG                                                >  SRR4302543.7094606/1‑94 (MQ=60)
                                               AAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTG                                                >  SRR4302543.1809637/1‑94 (MQ=60)
                                               AAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTG                                                >  SRR4302543.5991797/1‑94 (MQ=60)
                                                 AGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGAC                                              <  SRR4302543.4101815/94‑1 (MQ=60)
                                                   CAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGC                                            >  SRR4302543.5077878/1‑94 (MQ=60)
                                                   CAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGC                                            >  SRR4302543.3683744/1‑94 (MQ=60)
                                                   CAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGC                                            >  SRR4302543.4911035/1‑94 (MQ=60)
                                                    AATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCA                                           >  SRR4302543.4358241/1‑94 (MQ=60)
                                                     ATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAG                                          <  SRR4302543.4023068/94‑1 (MQ=60)
                                                     ATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAG                                          >  SRR4302543.6623231/1‑94 (MQ=60)
                                                      TCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGG                                         <  SRR4302543.6382653/94‑1 (MQ=60)
                                                       TTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGT                                        <  SRR4302543.1495417/94‑1 (MQ=60)
                                                       CTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGG                                        >  SRR4302543.6860982/1‑94 (MQ=60)
                                                        TTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTG                                       <  SRR4302543.6794428/94‑1 (MQ=60)
                                                          TATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCT                                     <  SRR4302543.6132277/94‑1 (MQ=60)
                                                           ATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTG                                    <  SRR4302543.3802876/94‑1 (MQ=60)
                                                           ATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTG                                    <  SRR4302543.3345769/94‑1 (MQ=60)
                                                             ACATCGGGCAGTCTCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGAT                                  <  SRR4302543.1922397/94‑1 (MQ=60)
                                                             ACATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGAT                                  <  SRR4302543.1549622/94‑1 (MQ=60)
                                                              CATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATA                                 >  SRR4302543.368014/1‑94 (MQ=60)
                                                              CATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATA                                 >  SRR4302543.5873841/1‑94 (MQ=60)
                                                              CATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATA                                 >  SRR4302543.4479819/1‑94 (MQ=60)
                                                               ATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATAT                                >  SRR4302543.5092592/1‑94 (MQ=60)
                                                               ATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATAT                                >  SRR4302543.3471817/1‑94 (MQ=60)
                                                               ATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATAT                                >  SRR4302543.4029495/1‑94 (MQ=60)
                                                               ATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATAT                                >  SRR4302543.7219824/1‑94 (MQ=60)
                                                               ATCGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATAT                                >  SRR4302543.7558477/1‑94 (MQ=60)
                                                                 CGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCA                              >  SRR4302543.160608/1‑94 (MQ=60)
                                                                 CGGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCA                              >  SRR4302543.3802601/1‑94 (MQ=60)
                                                                  GGGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCAC                             >  SRR4302543.4030674/1‑94 (MQ=60)
                                                                   GGCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGATATCACC                            >  SRR4302543.3444574/1‑94 (MQ=60)
                                                                    GCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCA                           >  SRR4302543.130328/1‑94 (MQ=60)
                                                                    GCAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCA                           <  SRR4302543.824174/94‑1 (MQ=60)
                                                                     CAGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGATATCACCAT                          <  SRR4302543.7445690/94‑1 (MQ=60)
                                                                      AGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGATATCACCATC                         <  SRR4302543.749962/94‑1 (MQ=60)
                                                                      AGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATC                         <  SRR4302543.7002275/94‑1 (MQ=60)
                                                                      AGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATC                         >  SRR4302543.7306765/1‑94 (MQ=60)
                                                                      AGTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATC                         >  SRR4302543.900368/1‑94 (MQ=60)
                                                                       GTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCG                        <  SRR4302543.3430540/94‑1 (MQ=60)
                                                                       GTCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGGGCTGATATCACCATCG                        >  SRR4302543.313002/1‑94 (MQ=60)
                                                                        TCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGA                       <  SRR4302543.1268776/94‑1 (MQ=60)
                                                                        TCGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGA                       <  SRR4302543.1268777/94‑1 (MQ=60)
                                                                         CGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGATATCACCATCGAC                      <  SRR4302543.3767486/94‑1 (MQ=60)
                                                                         CGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGAC                      >  SRR4302543.1294939/1‑94 (MQ=60)
                                                                         CGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGAC                      <  SRR4302543.4473153/94‑1 (MQ=60)
                                                                         CGCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGAC                      >  SRR4302543.1664300/1‑94 (MQ=60)
                                                                          CCTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACT                     <  SRR4302543.5946449/94‑1 (MQ=60)
                                                                           CTGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTG                    >  SRR4302543.7263425/1‑94 (MQ=60)
                                                                            TGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGT                   >  SRR4302543.7586379/1‑94 (MQ=60)
                                                                            TGCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGT                   >  SRR4302543.3808984/1‑94 (MQ=60)
                                                                             GCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTT                  <  SRR4302543.6279520/94‑1 (MQ=60)
                                                                             GCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTT                  <  SRR4302543.2264586/94‑1 (MQ=60)
                                                                             GCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTT                  <  SRR4302543.2005085/94‑1 (MQ=60)
                                                                             GCCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTT                  <  SRR4302543.1420953/94‑1 (MQ=60)
                                                                              CCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGATATCACCATCGACTGTTT                 >  SRR4302543.4104074/1‑94 (MQ=60)
                                                                              CCAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTT                 >  SRR4302543.3078421/1‑94 (MQ=60)
                                                                               CAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTG                >  SRR4302543.6577994/1‑94 (MQ=60)
                                                                               CAAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTG                <  SRR4302543.5163944/94‑1 (MQ=60)
                                                                                AAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGT               >  SRR4302543.2109321/1‑94 (MQ=60)
                                                                                AAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGA               >  SRR4302543.1897099/1‑94 (MQ=60)
                                                                                AAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGA               <  SRR4302543.1789588/94‑1 (MQ=60)
                                                                                AAGACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGA               >  SRR4302543.4090533/1‑94 (MQ=60)
                                                                                  GACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATT             <  SRR4302543.219384/94‑1 (MQ=60)
                                                                                  GACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATT             >  SRR4302543.3077638/1‑94 (MQ=60)
                                                                                  GACAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATT             <  SRR4302543.2567682/94‑1 (MQ=60)
                                                                                    CAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAG           <  SRR4302543.458743/94‑1 (MQ=60)
                                                                                    CAGTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAG           >  SRR4302543.553036/1‑94 (MQ=60)
                                                                                      GTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAC         <  SRR4302543.7580624/94‑1 (MQ=60)
                                                                                      GTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAC         >  SRR4302543.356571/1‑94 (MQ=60)
                                                                                      GTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAC         >  SRR4302543.6955914/1‑94 (MQ=60)
                                                                                      GTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAC         <  SRR4302543.7921807/94‑1 (MQ=60)
                                                                                      GTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAC         >  SRR4302543.6743842/1‑94 (MQ=60)
                                                                                      GTTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGAC         <  SRR4302543.120894/94‑1 (MQ=60)
                                                                                       TTGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACC        <  SRR4302543.918886/94‑1 (MQ=60)
                                                                                        TGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCA       <  SRR4302543.4050588/94‑1 (MQ=60)
                                                                                        TGTCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCA       <  SRR4302543.828558/94‑1 (MQ=60)
                                                                                          TCGCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGG     >  SRR4302543.4037322/1‑94 (MQ=60)
                                                                                            GCTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAG   >  SRR4302543.4385229/1‑94 (MQ=60)
                                                                                             CTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCGGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGC  <  SRR4302543.2869214/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACCACCACCACATCGGCGTCCATCGCTTTTTTACGTGCTTTGACCACAAAGCAATCTTTATACATCGGGCAGTCGCTGCCAAGACAGTTGTCGTTGGTGCTGGTGACCAGCGGCCACGCCTGTGAATCTTCCGCCACGCTGACGCAGGTGCTGATATCACCATCGACTGTTTGATTAGACCAGGAGC  >  NC_000913/1890673‑1890859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑