Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,930,097	(A)_7→6	intergenic (+14/+42)	purT → / ← eda	phosphoribosylglycinamide formyltransferase 2/2‑keto‑3‑deoxygluconate 6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,930,091	0	A	.	99.4%	806.9 / ‑2.7	179	intergenic (+8/+48)	purT/eda	phosphoribosylglycinamide formyltransferase 2/2‑keto‑3‑deoxygluconate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (69/109); total (69/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.05e-01

Only 100 of 211 total aligned reads displayed.

GTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG  >  NC_000913/1930000‑1930179

gTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaa                                                                                         <  1:7672532/93‑1 (MQ=255)

 tGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCata                                                                                        >  1:6146906/1‑91 (MQ=255)

 tGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaa                                                                                        >  1:483455/1‑93 (MQ=255)

    cACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaa                                                                                     <  1:833833/93‑1 (MQ=255)

    cACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaa                                                                                     <  1:3834127/93‑1 (MQ=255)

     aCTGGCTACTGGAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAGCCCGGCaaaaaac                                                                                    <  2:634174/93‑2 (MQ=255)

     aCTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACACGGCaaaaaac                                                                                    >  2:4982079/1‑92 (MQ=255)

      cTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacg                                                                                   >  2:450933/1‑91 (MQ=255)

      cTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacg                                                                                   >  1:7301877/1‑91 (MQ=255)

      cTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacg                                                                                   <  1:4718562/93‑3 (MQ=255)

        ggCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacgct                                                                                 >  2:89839/1‑89 (MQ=255)

        ggCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacgct                                                                                 >  2:462918/1‑89 (MQ=255)

          cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGTGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAc                                                                              <  2:7936026/93‑1 (MQ=255)

           tACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACa                                                                             >  2:6021657/1‑93 (MQ=255)

           tACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACa                                                                             >  2:7255665/1‑93 (MQ=255)

            aCTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaa                                                                            >  2:236651/1‑93 (MQ=255)

             cTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaa                                                                           >  1:1644684/1‑93 (MQ=255)

              tGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaa                                                                          >  2:4119013/1‑93 (MQ=255)

               gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                         <  2:2540258/93‑1 (MQ=255)

               gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                         <  1:3079577/93‑1 (MQ=255)

               gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                         <  2:2117581/93‑1 (MQ=255)

               gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                         <  1:234282/93‑1 (MQ=255)

               gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                         <  1:2233885/93‑1 (MQ=255)

                cAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAt                                                                        >  1:5670535/1‑93 (MQ=255)

                  gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGc                                                                      >  2:3630398/1‑93 (MQ=255)

                   ggcgtgtTGTTGACGCCATTGAACCCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                     <  2:3041817/90‑1 (MQ=255)

                   agagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                     >  2:6169798/1‑93 (MQ=255)

                   agagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                     <  1:553776/93‑1 (MQ=255)

                   agagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                     >  2:3921895/1‑93 (MQ=255)

                    gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                    <  1:6135547/93‑1 (MQ=255)

                      gtgtTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGa                                                                  <  2:5363751/93‑1 (MQ=255)

                       tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAt                                                                 <  2:7326893/93‑1 (MQ=255)

                       tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAt                                                                 <  2:2835778/93‑1 (MQ=255)

                         ttgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAttc                                                               >  1:5711855/1‑91 (MQ=255)

                         ttgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATcc                                                               >  2:7761664/1‑93 (MQ=255)

                          tgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCt                                                              <  1:3241511/93‑1 (MQ=255)

                           gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTc                                                             <  1:4328895/93‑1 (MQ=255)

                              gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTCCAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                          <  1:1333299/93‑1 (MQ=255)

                              gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                          <  2:7792084/93‑1 (MQ=255)

                               aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATc                                                         <  1:2959106/93‑1 (MQ=255)

                               aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATc                                                         >  2:2017378/1‑93 (MQ=255)

                               aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATc                                                         <  2:6725204/93‑1 (MQ=255)

                                cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCg                                                        <  1:7811433/93‑1 (MQ=255)

                                cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCg                                                        <  1:957529/93‑1 (MQ=255)

                                 gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCgg                                                       >  1:5958493/1‑93 (MQ=255)

                                  ccATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCggg                                                      >  2:329556/1‑93 (MQ=255)

                                    aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                    <  1:4166298/93‑1 (MQ=255)

                                    aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                    >  1:2450273/1‑93 (MQ=255)

                                     ttGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCTGC‑AAAAAACGCTACAAAAATGCGCGATCCTCGACGGGGCAt                                                   >  1:3394452/1‑93 (MQ=255)

                                      taaccgcgcgAAGCCCGCCGCGGGACAGGTAAAAGTACAGGGTAAAACCC‑GAAAAAAAACGCAAAAAAAAGGCCCGATCCTCGATCGGGCAtt                                                  <  2:3142789/89‑1 (MQ=255)

                                      tGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtt                                                  <  2:4129456/93‑1 (MQ=255)

                                      tGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCCATTCTCGATCGGGGAtt                                                  >  2:19921/1‑93 (MQ=255)

                                       gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt                                                 <  2:882896/93‑1 (MQ=255)

                                       gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt                                                 <  2:1121418/93‑1 (MQ=255)

                                       gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt                                                 <  1:5196999/93‑1 (MQ=255)

                                           gcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGGTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCCGGCATTTTTAc                                             >  1:145545/1‑93 (MQ=255)

                                              cgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACttt                                          <  1:6822079/93‑1 (MQ=255)

                                               gAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGTATTTTGACtttt                                         >  2:7738651/1‑93 (MQ=255)

                                               gAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt                                         <  2:6216455/93‑1 (MQ=255)

                                                aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                        >  1:2606773/1‑93 (MQ=255)

                                                aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                        >  1:6991224/1‑93 (MQ=255)

                                                 aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATttttacttttac                                       >  2:3260940/1‑93 (MQ=255)

                                                 aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                       <  2:2473288/93‑1 (MQ=255)

                                                  gCACGCCGCCGGACAGGTAAAAGTGCAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                      <  1:2567027/93‑1 (MQ=255)

                                                  gCACCCGGCGGGACAGTTAAAGGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                      <  2:4315480/93‑1 (MQ=255)

                                                  ccACGCCGCCGGACAGGTAAAAGTCCAGGGTAAAACCCGAC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                      <  1:136122/92‑1 (MQ=255)

                                                    aCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGc                                    <  1:1895073/93‑1 (MQ=255)

                                                      gccgccGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCtt                                  <  1:392624/93‑1 (MQ=255)

                                                       ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                 <  1:1251686/93‑1 (MQ=255)

                                                       ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                 <  2:2287861/93‑1 (MQ=255)

                                                       ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                 <  2:5164125/93‑1 (MQ=255)

                                                       ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                 <  1:7737006/93‑1 (MQ=255)

                                                       ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                 <  1:187914/93‑1 (MQ=255)

                                                        cgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAg                                <  2:2718926/93‑1 (MQ=255)

                                                         gccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAACGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                               <  1:2017379/93‑1 (MQ=255)

                                                          ccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcg                              <  1:6402173/93‑1 (MQ=255)

                                                           cgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                             <  1:6611293/93‑1 (MQ=255)

                                                            ggACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                            >  1:6447079/1‑93 (MQ=255)

                                                               cAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTc                         <  2:1170848/93‑1 (MQ=255)

                                                                  gTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTTTTACAGCTTAGCGCCTTCTAc                      >  1:4511509/1‑93 (MQ=255)

                                                                  gTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAc                      <  1:6322066/93‑1 (MQ=255)

                                                                   tAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTTCAGCTTAGCGCCTTCTACa                     >  2:7420620/1‑93 (MQ=255)

                                                                    aaaaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAg                    <  2:1390482/93‑1 (MQ=255)

                                                                    aaaaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAg                    <  1:4045707/93‑1 (MQ=255)

                                                                      aaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCt                  >  2:7824724/1‑93 (MQ=255)

                                                                        gTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                <  2:2214731/93‑1 (MQ=255)

                                                                        gTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                >  1:7421308/1‑93 (MQ=255)

                                                                        gTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                <  2:759495/93‑1 (MQ=255)

                                                                          tcAGGGGTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAc              <  1:4106997/92‑1 (MQ=255)

                                                                           cAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcg             >  1:7641769/1‑93 (MQ=255)

                                                                             gggTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTTAcgcg           >  2:4760760/1‑93 (MQ=255)

                                                                             gggTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgcg           >  1:583298/1‑93 (MQ=255)

                                                                              ggTTAAACCCGGC‑AAAAAACGCTACAAAAATGGCCGATCCTCGATCCGGCAATTTGACTTTTATAGCTTGGCGCCTTCTACCGCCTCACgcgc          >  2:4304415/1‑93 (MQ=255)

                                                                                ttAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCa        <  2:4539897/93‑1 (MQ=255)

                                                                                 tAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAg       <  2:5904644/93‑1 (MQ=255)

                                                                                  aaaCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGc      <  1:667554/93‑1 (MQ=255)

                                                                                   aaCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCCATCGGGCCTTTTTACTTTTTTAGCTTAGCGCCTTCTACAGCTTTCCGCGCCCGCt     >  1:5736117/1‑93 (MQ=255)

                                                                                     cccGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa   <  1:3073850/93‑1 (MQ=255)

                                                                                      cccgCAAAAAAACAAGACAAAACTCCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa   <  2:6950946/90‑1 (MQ=255)

                                                                                      ccGGC‑AAAAAACGCTACAAAAATGCCCTATCCTCGATCGGGCATTTTCACCTTTAAAGCTTAACCCCTCTTACAGCTTCACGCGCCAAATTAg  >  1:2216438/1‑93 (MQ=255)

GTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG  >  NC_000913/1930000‑1930179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCG  >  NC_000913/1930004‑1930190

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAC                                                                                               <  SRR4302543.7448992/94‑1 (MQ=60)

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAC                                                                                               <  SRR4302543.841188/94‑1 (MQ=60)

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAC                                                                                               <  SRR4302543.3867958/94‑1 (MQ=60)

  CTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGC                                                                                            <  SRR4302543.4761675/94‑1 (MQ=60)

    GGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTA                                                                                          <  SRR4302543.6042776/94‑1 (MQ=60)

      CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACA                                                                                        <  SRR4302543.7593293/94‑1 (MQ=60)

        ACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAA                                                                                      >  SRR4302543.1659372/1‑94 (MQ=60)

         CTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAA                                                                                     >  SRR4302543.4405902/1‑94 (MQ=60)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                   <  SRR4302543.236305/94‑1 (MQ=60)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                   <  SRR4302543.236306/94‑1 (MQ=60)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                   <  SRR4302543.2254090/94‑1 (MQ=60)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                   >  SRR4302543.5722292/1‑94 (MQ=60)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                   <  SRR4302543.3107270/94‑1 (MQ=60)

            CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                  >  SRR4302543.5751005/1‑94 (MQ=60)

            CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                  <  SRR4302543.6196700/94‑1 (MQ=60)

             AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                 <  SRR4302543.6468420/94‑1 (MQ=60)

              GAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCC                                                                                <  SRR4302543.736569/94‑1 (MQ=60)

               AGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCC                                                                               <  SRR4302543.558864/94‑1 (MQ=60)

                GAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCG                                                                              <  SRR4302543.5303376/94‑1 (MQ=60)

                GAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCG                                                                              <  SRR4302543.3564711/94‑1 (MQ=60)

                GAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCG                                                                              <  SRR4302543.6193670/94‑1 (MQ=60)

                 AGTGTTGTTGACGCCATTGAGCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCG                                                                              <  SRR4302543.6408166/94‑1 (MQ=60)

                 AGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGA                                                                             >  SRR4302543.3885601/1‑94 (MQ=60)

                  GTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAT                                                                            <  SRR4302543.4577168/94‑1 (MQ=60)

                    GTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATTC                                                                          >  SRR4302543.5763968/1‑94 (MQ=60)

                    GTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGACCC                                                                          >  SRR4302543.5763969/1‑94 (MQ=60)

                     TTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCTATCCT                                                                         <  SRR4302543.3104360/94‑1 (MQ=60)

                     TTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCT                                                                         <  SRR4302543.56918/94‑1 (MQ=60)

                     TTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCT                                                                         <  SRR4302543.4877774/94‑1 (MQ=60)

                      TGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTC                                                                        <  SRR4302543.3270831/94‑1 (MQ=60)

                       GTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCG                                                                       >  SRR4302543.6722180/1‑94 (MQ=60)

                       GTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCG                                                                       <  SRR4302543.4368180/94‑1 (MQ=60)

                         TGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAT                                                                     >  SRR4302543.7784592/1‑94 (MQ=60)

                          GACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTCCAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATC                                                                    <  SRR4302543.1345182/94‑1 (MQ=60)

                          GACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATC                                                                    <  SRR4302543.1345183/94‑1 (MQ=60)

                           ACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCG                                                                   <  SRR4302543.2985969/94‑1 (MQ=60)

                            CGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGG                                                                  >  SRR4302543.6013030/1‑94 (MQ=60)

                            CGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGG                                                                  <  SRR4302543.965919/94‑1 (MQ=60)

                            CGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGG                                                                  <  SRR4302543.7885056/94‑1 (MQ=60)

                             GCCATTGAACGCGCGAAGCTCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGG                                                                 <  SRR4302543.7910106/94‑1 (MQ=60)

                               CATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCA                                                               >  SRR4302543.2472375/1‑94 (MQ=60)

                                ATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCTGC‑AAAAAACGCTACAAAAATGCGCGATCCTCGACGGGGCAT                                                              >  SRR4302543.3424886/1‑94 (MQ=60)

                                ATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAT                                                              <  SRR4302543.4204021/94‑1 (MQ=60)

                                ATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAT                                                              <  SRR4302543.4204020/94‑1 (MQ=60)

                                ATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAT                                                              >  SRR4302543.7852533/1‑94 (MQ=60)

                                  TGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTT                                                            <  SRR4302543.2618418/94‑1 (MQ=60)

                                   GAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTT                                                           <  SRR4302543.5243857/94‑1 (MQ=60)

                                      CGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGGTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCCGGCATTTTTAC                                                        >  SRR4302543.146811/1‑94 (MQ=60)

                                      CGCGCGAAGCACGCCGCCAGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGAC                                                        <  SRR4302543.6959894/94‑1 (MQ=60)

                                          caaacCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCGGGC‑AAAAAACGCAAAAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTT                                                    <  SRR4302543.1494529/89‑1 (MQ=60)

                                          CGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTT                                                    <  SRR4302543.6886484/94‑1 (MQ=60)

                                           GAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTA                                                   >  SRR4302543.7057023/1‑94 (MQ=60)

                                           GAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTA                                                   <  SRR4302543.876237/94‑1 (MQ=60)

                                           GAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTA                                                   >  SRR4302543.2630451/1‑94 (MQ=60)

                                             AGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACA                                                 <  SRR4302543.7708324/94‑1 (MQ=60)

                                              GCACGCCGCCGGACAGGTAAAAGTGCAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAG                                                <  SRR4302543.2590469/94‑1 (MQ=60)

                                              CCACGCCGCCGGACAGGTAAAAGTCCAGGGTAAAACCCGAC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAG                                                <  SRR4302543.137324/94‑1 (MQ=60)

                                                ACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCT                                              <  SRR4302543.1912117/94‑1 (MQ=60)

                                                  GCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTA                                            <  SRR4302543.396246/94‑1 (MQ=60)

                                                   CCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAG                                           <  SRR4302543.7810108/94‑1 (MQ=60)

                                                   CCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAG                                           <  SRR4302543.1262909/94‑1 (MQ=60)

                                                   CCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAG                                           <  SRR4302543.189511/94‑1 (MQ=60)

                                                   CCGCCGGACAGGTAAAAGCACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAG                                           <  SRR4302543.2303620/94‑1 (MQ=60)

                                                    CGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGC                                          >  SRR4302543.2818083/1‑94 (MQ=60)

                                                     GCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCG                                         <  SRR4302543.229450/94‑1 (MQ=60)

                                                     GCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAACGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCG                                         <  SRR4302543.2035508/94‑1 (MQ=60)

                                                      CCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGC                                        <  SRR4302543.6462446/94‑1 (MQ=60)

                                                       CGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCC                                       >  SRR4302543.6507820/1‑94 (MQ=60)

                                                       CGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCC                                       <  SRR4302543.6673605/94‑1 (MQ=60)

                                                        GGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCT                                      >  SRR4302543.2581636/1‑94 (MQ=60)

                                                         GACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCCATCTGGCATTTTGTCTTTTTCAGCTTAGTGCCTT                                     >  SRR4302543.3166287/1‑94 (MQ=60)

                                                          ACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCCGGCATTTTGACTTTTACAGCTTAGCGCCTTC                                    >  SRR4302543.421680/1‑94 (MQ=60)

                                                           CAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAACCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCT                                   >  SRR4302543.4016596/1‑94 (MQ=60)

                                                            AGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCcgtccccgcaccgggcgttttactttttttacttcgcgccttttt                                  >  SRR4302543.1435056/1‑49 (MQ=60)

                                                            AGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTA                                  >  SRR4302543.6216793/1‑94 (MQ=60)

                                                             GGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTTTTACAGCTTAGCGCCTTCTAC                                 >  SRR4302543.4552536/1‑94 (MQ=60)

                                                             GGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAC                                 <  SRR4302543.1333754/94‑1 (MQ=60)

                                                              GTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACA                                <  SRR4302543.6381514/94‑1 (MQ=60)

                                                              GTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACA                                >  SRR4302543.5905179/1‑94 (MQ=60)

                                                                AAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGC                              <  SRR4302543.4081912/94‑1 (MQ=60)

                                                                  AAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTT                            <  SRR4302543.3095909/94‑1 (MQ=60)

                                                                   AGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCTCCTTCTACAGCTTC                           >  SRR4302543.2206113/1‑94 (MQ=60)

                                                                   AGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTC                           >  SRR4302543.7492492/1‑94 (MQ=60)

                                                                    GTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATTCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTTTACACCTTCA                          >  SRR4302543.1905030/1‑94 (MQ=60)

                                                                    GTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCA                          >  SRR4302543.6550829/1‑94 (MQ=60)

                                                                      TCAGGGGTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACG                        <  SRR4302543.4143945/94‑1 (MQ=60)

                                                                      ACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACG                        <  SRR4302543.4143944/94‑1 (MQ=60)

                                                                      ACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACG                        >  SRR4302543.3380845/1‑94 (MQ=60)

                                                                      ACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACG                        >  SRR4302543.7714251/1‑94 (MQ=60)

                                                                        AGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCG                      >  SRR4302543.588623/1‑94 (MQ=60)

                                                                              AAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCT                <  SRR4302543.673698/94‑1 (MQ=60)

                                                                              AAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCT                <  SRR4302543.1484281/94‑1 (MQ=60)

                                                                              AAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCCATCGGGCCTTTTTACTTTTTTAGCTTAGCGCCTTCTACAGCTTTCCGCGCCCGCT                >  SRR4302543.5788437/1‑94 (MQ=54)

                                                                               AACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTT               >  SRR4302543.6839296/1‑94 (MQ=60)

                                                                               AACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTT               >  SRR4302543.4910940/1‑94 (MQ=60)

                                                                               AACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTT               >  SRR4302543.3729165/1‑94 (MQ=60)

                                                                                 CCCGGC‑AAAAAACGCTACAAAAATGCCCTATCCTCGATCGGGCATTTTCACCTTTAAAGCTTAACCCCTCTTACAGCTTCACGCGCCAAATTAG             >  SRR4302543.2236421/1‑94 (MQ=37)

                                                                                 CCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG             <  SRR4302543.6260222/94‑1 (MQ=60)

                                                                                 CCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG             <  SRR4302543.3101489/94‑1 (MQ=60)

                                                                                 CCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG             >  SRR4302543.2236423/1‑94 (MQ=60)

                                                                                  CCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGT            <  SRR4302543.1935569/94‑1 (MQ=60)

                                                                                  CCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGT            >  SRR4302543.6916148/1‑94 (MQ=60)

                                                                                    GGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAA          <  SRR4302543.1078139/94‑1 (MQ=60)

                                                                                      gcAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT         >  SRR4302543.3765238/3‑94 (MQ=60)

                                                                                      gcAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT         >  SRR4302543.3765237/3‑94 (MQ=60)

                                                                                      gcAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT         <  SRR4302543.3298745/92‑1 (MQ=60)

                                                                                       CAAAACACGCTACAAACATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATG        <  SRR4302543.7103184/94‑1 (MQ=60)

                                                                                       CAAAAACCGCTAAAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATG        <  SRR4302543.1555576/94‑1 (MQ=60)

                                                                                       CAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATG        >  SRR4302543.5017212/1‑94 (MQ=60)

                                                                                       CAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATG        <  SRR4302543.4943658/94‑1 (MQ=60)

                                                                                        AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATTC       >  SRR4302543.3721815/1‑94 (MQ=60)

                                                                                         AAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCG      >  SRR4302543.2157107/1‑94 (MQ=60)

                                                                                         AAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCG      >  SRR4302543.3973368/1‑94 (MQ=60)

                                                                                         AAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCG      <  SRR4302543.3179532/94‑1 (MQ=60)

                                                                                         AAAAACGCTACAAAAATGCCCGATCCCCGATCAGGCACTTTGACTCTTACAGCTTGGCGCCTTCTAaacccttcccgcccgacttcgaaatgag      >  SRR4302543.6120139/1‑66 (MQ=50)

                                                                                          AAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGG     <  SRR4302543.777952/94‑1 (MQ=60)

                                                                                           AAACGCTACAAAAATGCCCGATCCTCGATCGGGGATTTTGACTTTTAAAGCTTAGCGCCTTCTACAGCTTCACGCGCCCGCTTAGTAATGGGGT    >  SRR4302543.1820973/1‑94 (MQ=60)

                                                                                           AAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGT    <  SRR4302543.209856/94‑1 (MQ=60)

                                                                                            AACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC   >  SRR4302543.4958269/1‑94 (MQ=60)

                                                                                            AACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCCTAGTAATGCGGTC   >  SRR4302543.6506609/1‑94 (MQ=60)

                                                                                             ACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCG  <  SRR4302543.90679/94‑1 (MQ=60)

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCG  >  NC_000913/1930004‑1930190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`