Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,028,708 A→G V78A (GTG→GCG)  vsr ← DNA mismatch endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,7080AG98.9% 830.7 / ‑1.7 269V78A (GTG→GCG) vsrDNA mismatch endonuclease
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (120/146);  total (122/147)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 273 total aligned reads displayed.

CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGT  >  NC_000913/2028618‑2028797
                                                                                          |                                                                                         
ccGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCt                                                                                         <  2:1560610/93‑1 (MQ=255)
  gAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCACGCGCttt                                                                                       >  2:2278534/1‑93 (MQ=255)
   aGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTa                                                                                      <  1:740711/93‑1 (MQ=255)
   aGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTa                                                                                      >  1:3375427/1‑93 (MQ=255)
    gTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACCTTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaa                                                                                     >  2:4059903/1‑93 (MQ=255)
    gTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCATGCGCTTTaa                                                                                     >  2:4227524/1‑93 (MQ=255)
    gTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaa                                                                                     <  2:3987784/93‑1 (MQ=255)
    gTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaa                                                                                     <  1:1879245/93‑1 (MQ=255)
     ttCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaaa                                                                                    <  2:7385653/93‑1 (MQ=255)
     ttCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaaa                                                                                    <  2:2664872/93‑1 (MQ=255)
     ttCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaaa                                                                                    <  2:3175699/93‑1 (MQ=255)
       ccTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACa                                                                                  <  2:7918863/93‑1 (MQ=255)
         tGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGa                                                                                <  1:6363435/93‑1 (MQ=255)
         tGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGa                                                                                <  1:3953351/93‑1 (MQ=255)
           cAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATa                                                                              <  1:2039190/93‑1 (MQ=255)
           cAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATa                                                                              >  2:3498173/1‑93 (MQ=255)
            aaGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAg                                                                             >  2:6697926/1‑93 (MQ=255)
              gCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCa                                                                           <  2:2451092/93‑1 (MQ=255)
              gCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCa                                                                           >  1:2123646/1‑93 (MQ=255)
                gACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGt                                                                         <  2:4139547/93‑1 (MQ=255)
                 aCTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGTGCCTTAAACAGATAGCAGtg                                                                        >  2:7526724/1‑93 (MQ=255)
                 aCTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtg                                                                        >  1:4078132/1‑93 (MQ=255)
                 aCTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtg                                                                        >  1:7543595/1‑93 (MQ=255)
                  cTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCATGCGCTTTAAACAGATAGCAGtga                                                                       >  2:4290159/1‑93 (MQ=255)
                  cTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtga                                                                       >  2:3036178/1‑93 (MQ=255)
                   tGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgat                                                                      >  1:3820754/1‑93 (MQ=255)
                     atatCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatga                                                                    <  2:4958429/93‑1 (MQ=255)
                      tatCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgat                                                                   <  2:1113562/93‑1 (MQ=255)
                      tatCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgat                                                                   <  2:520693/93‑1 (MQ=255)
                       atCGCGGCGATCGCGCTCAACATTTTTCCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatg                                                                  <  1:2296592/93‑1 (MQ=255)
                        tCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatga                                                                 <  1:6568911/93‑1 (MQ=255)
                        tCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatga                                                                 <  2:906229/93‑1 (MQ=255)
                        tCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatga                                                                 <  1:1811823/93‑1 (MQ=255)
                        tCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatga                                                                 <  2:5331828/93‑1 (MQ=255)
                           cggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGc                                                              <  1:7821682/93‑1 (MQ=255)
                           cggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGc                                                              <  1:6466816/93‑1 (MQ=255)
                               gATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGa                                                          >  1:6166107/1‑93 (MQ=255)
                                aTCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGaa                                                         >  1:4440118/1‑93 (MQ=255)
                                 tCGCGCTCAACATTTTTACCTATCTTCTCAAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAag                                                        >  1:2651127/1‑93 (MQ=255)
                                  cgcgCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagc                                                       >  1:6988402/1‑93 (MQ=255)
                                   gcgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagca                                                      >  2:1914052/1‑93 (MQ=255)
                                    cgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcag                                                     <  1:42169/93‑1 (MQ=255)
                                    cgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcag                                                     <  2:7217709/93‑1 (MQ=255)
                                     gcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcagc                                                    <  1:6072538/93‑1 (MQ=255)
                                      cTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGcc                                                   >  1:6856957/1‑93 (MQ=255)
                                       tCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCa                                                  >  2:2100684/1‑93 (MQ=255)
                                       tCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCa                                                  <  1:5997733/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                 <  1:4646322/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                 <  1:7913775/93‑1 (MQ=255)
                                         aaCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATg                                                <  2:5949287/93‑1 (MQ=255)
                                         aaCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATg                                                >  2:7849797/1‑93 (MQ=255)
                                           cATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATggg                                              >  2:4617058/1‑93 (MQ=255)
                                           cATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATggg                                              >  2:4456495/1‑93 (MQ=255)
                                             tttttACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTa                                            >  2:6356737/1‑93 (MQ=255)
                                                 tACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAata                                        >  2:2762161/1‑93 (MQ=255)
                                                 tACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAata                                        <  1:3183177/93‑1 (MQ=255)
                                                 tACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAata                                        >  1:1157375/1‑93 (MQ=255)
                                                 tACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAata                                        <  1:2728769/93‑1 (MQ=255)
                                                 tACCCATCTTCTCCAGCAAGAACTCGGGTCGAGTCGCAGGCACTTTAAAAAGATAGCAGGGATGATGATGCCAGAAGCAGCCATGGGTAAata                                        <  2:5490197/93‑1 (MQ=255)
                                                  tccTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAatat                                       <  2:1759318/92‑1 (MQ=255)
                                                  aCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAatat                                       <  1:5759011/93‑1 (MQ=255)
                                                   ccTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATc                                      <  2:5808537/93‑1 (MQ=255)
                                                     tATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCAc                                    >  1:3476107/1‑93 (MQ=255)
                                                        cttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCa                                 <  2:3614518/93‑1 (MQ=255)
                                                          tctcCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGc                               <  1:5324623/93‑1 (MQ=255)
                                                          tctcCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGc                               <  1:5049193/93‑1 (MQ=255)
                                                          tctcCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGc                               <  2:82973/93‑1 (MQ=255)
                                                            tcCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGa                             <  1:7164384/93‑1 (MQ=255)
                                                              cagccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGata                           <  1:5360686/93‑1 (MQ=255)
                                                              cagccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGata                           <  1:4781594/93‑1 (MQ=255)
                                                               agacagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGatat                          >  2:7714859/4‑93 (MQ=255)
                                                                 ccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtc                        <  1:6736031/93‑1 (MQ=255)
                                                                  cagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtca                       <  1:129294/93‑1 (MQ=255)
                                                                  cagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtca                       <  2:2836648/93‑1 (MQ=255)
                                                                  cagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtca                       <  2:1712255/93‑1 (MQ=255)
                                                                  cagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtca                       <  1:3612185/93‑1 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                      >  2:4356042/1‑93 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                      >  2:1004175/1‑93 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                      >  1:963967/1‑93 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                      <  2:701099/93‑1 (MQ=255)
                                                                    gAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcatc                     >  1:22252/1‑93 (MQ=255)
                                                                    gAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcatc                     <  1:3105023/93‑1 (MQ=255)
                                                                           gTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACa              >  1:4445026/1‑93 (MQ=255)
                                                                              cGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACaaaa           >  1:4286442/1‑93 (MQ=255)
                                                                              cGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACaaaa           >  1:6820903/1‑93 (MQ=255)
                                                                               gAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAt          >  1:1429469/1‑93 (MQ=255)
                                                                               gAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAt          >  2:2912422/1‑93 (MQ=255)
                                                                               gAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAt          >  2:1414300/1‑93 (MQ=255)
                                                                                aGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATc         >  2:5963013/1‑93 (MQ=255)
                                                                                 gTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATcc        >  1:4174982/1‑93 (MQ=255)
                                                                                 gTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATcc        <  2:4666538/93‑1 (MQ=255)
                                                                                 gTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATcc        <  1:1758696/93‑1 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg       >  1:4839183/1‑93 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg       <  2:6812425/93‑1 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg       <  2:2226973/93‑1 (MQ=255)
                                                                                   cGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCgg      >  1:7077545/1‑93 (MQ=255)
                                                                                    ccAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGa     <  1:6240872/92‑1 (MQ=255)
                                                                                     cAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAc    <  2:5880356/93‑1 (MQ=255)
                                                                                      aGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACg   <  2:1102805/93‑1 (MQ=255)
                                                                                       ggCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGt  >  2:6126087/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGT  >  NC_000913/2028618‑2028797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  >  NC_000913/2028615‑2028801
                                                                                             |                                                                                             
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCG                                                                                               <  SRR4302543.101238/94‑1 (MQ=60)
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCG                                                                                               >  SRR4302543.3303433/1‑94 (MQ=60)
 AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302543.7184475/1‑94 (MQ=60)
 AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302543.640896/1‑94 (MQ=60)
 AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302543.4043647/1‑94 (MQ=60)
 AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302543.3971574/1‑94 (MQ=60)
 AGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGACAGAACTCGGTTCGAGTCGCAGGCGC                                                                                              >  SRR4302543.4530458/1‑94 (MQ=60)
   CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTT                                                                                            >  SRR4302543.4483200/1‑94 (MQ=60)
     GAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTA                                                                                          >  SRR4302543.4289453/1‑94 (MQ=60)
     GAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTA                                                                                          >  SRR4302543.3405727/1‑94 (MQ=60)
      AGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAA                                                                                         >  SRR4302543.6085289/1‑94 (MQ=60)
      AGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAA                                                                                         >  SRR4302543.2274813/1‑94 (MQ=60)
      AGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAA                                                                                         <  SRR4302543.747327/94‑1 (MQ=60)
       GTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAA                                                                                        <  SRR4302543.1896130/94‑1 (MQ=60)
        TTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAAC                                                                                       >  SRR4302543.4703930/1‑94 (MQ=60)
          CCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAG                                                                                     <  SRR4302543.1450148/94‑1 (MQ=60)
          CCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAG                                                                                     <  SRR4302543.596138/94‑1 (MQ=60)
          CCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAG                                                                                     <  SRR4302543.6842047/94‑1 (MQ=60)
           CTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGA                                                                                    >  SRR4302543.7949652/1‑94 (MQ=60)
            TGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAT                                                                                   <  SRR4302543.3988334/94‑1 (MQ=60)
            TGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAT                                                                                   <  SRR4302543.6423273/94‑1 (MQ=60)
             GCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATA                                                                                  >  SRR4302543.423606/1‑94 (MQ=60)
             GCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACGCGGTTCGAGTCGCAGGCGCTTTAAACAGATA                                                                                  <  SRR4302543.3517510/94‑1 (MQ=60)
              CAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAG                                                                                 <  SRR4302543.2057486/94‑1 (MQ=60)
              CAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAG                                                                                 <  SRR4302543.423516/94‑1 (MQ=60)
              CAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAG                                                                                 <  SRR4302543.2436183/94‑1 (MQ=60)
              CAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGACCGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAG                                                                                 >  SRR4302543.1713488/1‑94 (MQ=60)
              CAAGCGACTGATAGCGCGACGATCGCGCTCATCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGTCGCTTTAAACAGATAG                                                                                 <  SRR4302543.4466733/94‑1 (MQ=60)
               AAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGGCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGC                                                                                >  SRR4302543.7930263/1‑94 (MQ=60)
                AGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCA                                                                               >  SRR4302543.2142658/1‑94 (MQ=60)
                AGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCA                                                                               <  SRR4302543.1808102/94‑1 (MQ=60)
                AGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCA                                                                               >  SRR4302543.6310818/1‑94 (MQ=60)
                   GACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTG                                                                            >  SRR4302543.7615477/1‑94 (MQ=60)
                   GACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTG                                                                            >  SRR4302543.4114692/1‑94 (MQ=60)
                   GACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGACAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTG                                                                            >  SRR4302543.4535114/1‑94 (MQ=60)
                     CTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGCT                                                                          >  SRR4302543.3854444/1‑94 (MQ=60)
                     CTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGAT                                                                          >  SRR4302543.3854445/1‑94 (MQ=60)
                      TGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATG                                                                         <  SRR4302543.4843313/94‑1 (MQ=60)
                        ATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGAT                                                                       >  SRR4302543.7063657/1‑94 (MQ=60)
                          ATCGCGGCGATCGCGCTCAACATTTTTCCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGA                                                                     <  SRR4302543.2317538/94‑1 (MQ=60)
                          ATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGA                                                                     <  SRR4302543.5170658/94‑1 (MQ=60)
                           TCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGAT                                                                    <  SRR4302543.6630752/94‑1 (MQ=60)
                           TCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGAT                                                                    <  SRR4302543.2115254/94‑1 (MQ=60)
                           TCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGAT                                                                    <  SRR4302543.1828070/94‑1 (MQ=60)
                            CGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATG                                                                   <  SRR4302543.6908036/94‑1 (MQ=60)
                            CGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATG                                                                   <  SRR4302543.1874976/94‑1 (MQ=60)
                              CGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCC                                                                 <  SRR4302543.6527743/94‑1 (MQ=60)
                              CGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCC                                                                 <  SRR4302543.7895365/94‑1 (MQ=60)
                               GGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCA                                                                <  SRR4302543.990402/94‑1 (MQ=60)
                                 CGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGA                                                              >  SRR4302543.6224453/1‑94 (MQ=60)
                                  ccgatctccactctTTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAA                                                             >  SRR4302543.5157118/15‑94 (MQ=60)
                                  GATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAA                                                             >  SRR4302543.4480325/1‑94 (MQ=60)
                                   ATCGCGCTCAACATTTTTACCTATCTTCTCAAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                            >  SRR4302543.2675163/1‑94 (MQ=60)
                                    TCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGC                                                           >  SRR4302543.7054171/1‑94 (MQ=60)
                                    TCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGC                                                           <  SRR4302543.4845539/94‑1 (MQ=60)
                                    TCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATCATGCCAGAAGA                                                           >  SRR4302543.4205646/1‑94 (MQ=60)
                                     CGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCA                                                          <  SRR4302543.7236175/94‑1 (MQ=60)
                                      GCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCTGAAGCAG                                                         >  SRR4302543.3876328/1‑94 (MQ=60)
                                       CGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGC                                                        <  SRR4302543.42555/94‑1 (MQ=60)
                                        GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                       >  SRR4302543.7028051/1‑94 (MQ=60)
                                        GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                       <  SRR4302543.6130091/94‑1 (MQ=60)
                                        GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                       >  SRR4302543.6323572/1‑94 (MQ=60)
                                        GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                       >  SRR4302543.6921604/1‑94 (MQ=60)
                                          TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                     <  SRR4302543.1398846/94‑1 (MQ=60)
                                          TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                     <  SRR4302543.6052777/94‑1 (MQ=60)
                                          TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                     <  SRR4302543.141491/94‑1 (MQ=60)
                                          TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                     <  SRR4302543.3498165/94‑1 (MQ=60)
                                           CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                    >  SRR4302543.3277994/1‑94 (MQ=60)
                                           CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                    >  SRR4302543.6883923/1‑94 (MQ=60)
                                           CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                    <  SRR4302543.4688595/94‑1 (MQ=60)
                                           CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                    <  SRR4302543.7988122/94‑1 (MQ=60)
                                             ACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGG                                                  <  SRR4302543.662537/94‑1 (MQ=60)
                                             ACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGG                                                  <  SRR4302543.7160627/94‑1 (MQ=60)
                                              CATTTTTTCCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGT                                                 <  SRR4302543.5689519/94‑1 (MQ=60)
                                              CATGTTTACCTATCTGCTCCAGCCAGACCGCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGT                                                 <  SRR4302543.3190781/94‑1 (MQ=60)
                                               ATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTA                                                >  SRR4302543.3555586/1‑94 (MQ=60)
                                               ATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTA                                                >  SRR4302543.413110/1‑94 (MQ=60)
                                                TTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAA                                               <  SRR4302543.6260325/94‑1 (MQ=60)
                                                   TTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATA                                            >  SRR4302543.1167911/1‑94 (MQ=60)
                                                   TTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATA                                            >  SRR4302543.3993100/1‑94 (MQ=60)
                                                    TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           <  SRR4302543.3211885/94‑1 (MQ=60)
                                                    TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           <  SRR4302543.2753598/94‑1 (MQ=60)
                                                    TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           <  SRR4302543.6221175/94‑1 (MQ=60)
                                                    TACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATAT                                           <  SRR4302543.7700931/94‑1 (MQ=60)
                                                     ACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATC                                          <  SRR4302543.5811481/94‑1 (MQ=60)
                                                      CCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCA                                         >  SRR4302543.2691652/1‑94 (MQ=60)
                                                       CTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCAC                                        >  SRR4302543.3507241/1‑94 (MQ=60)
                                                        TATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACG                                       <  SRR4302543.4312568/94‑1 (MQ=60)
                                                        TATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACG                                       <  SRR4302543.5290642/94‑1 (MQ=60)
                                                             TCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCG                                  <  SRR4302543.5095101/94‑1 (MQ=60)
                                                             TCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCG                                  <  SRR4302543.5372764/94‑1 (MQ=60)
                                                              CTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGA                                 >  SRR4302543.7839241/1‑94 (MQ=60)
                                                              CTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGA                                 >  SRR4302543.7186732/1‑94 (MQ=60)
                                                               TCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGAT                                <  SRR4302543.7233977/94‑1 (MQ=60)
                                                               TCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGAT                                <  SRR4302543.3773652/94‑1 (MQ=60)
                                                                CCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATA                               >  SRR4302543.6987266/1‑94 (MQ=60)
                                                                 CAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATAT                              <  SRR4302543.5409200/94‑1 (MQ=60)
                                                                 CAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATAT                              <  SRR4302543.4825296/94‑1 (MQ=60)
                                                                  AGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATT                             <  SRR4302543.3960346/94‑1 (MQ=60)
                                                                  AGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATT                             <  SRR4302543.2077839/94‑1 (MQ=60)
                                                                    CCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCA                           <  SRR4302543.6799659/94‑1 (MQ=60)
                                                                     CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          >  SRR4302543.972428/1‑94 (MQ=60)
                                                                     CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          <  SRR4302543.3644035/94‑1 (MQ=60)
                                                                     CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          <  SRR4302543.130452/94‑1 (MQ=60)
                                                                     CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          <  SRR4302543.2024101/94‑1 (MQ=60)
                                                                     CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          <  SRR4302543.2258857/94‑1 (MQ=60)
                                                                     CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                          >  SRR4302543.3382577/1‑94 (MQ=60)
                                                                      AGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATC                         >  SRR4302543.579547/1‑94 (MQ=60)
                                                                      AGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATC                         >  SRR4302543.22458/1‑94 (MQ=60)
                                                                      AGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATC                         >  SRR4302543.1261024/1‑94 (MQ=60)
                                                                       GAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCA                        <  SRR4302543.3132954/94‑1 (MQ=60)
                                                                       GAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCA                        <  SRR4302543.7066844/94‑1 (MQ=60)
                                                                       GAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCA                        <  SRR4302543.7606089/94‑1 (MQ=60)
                                                                             GGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACA                  >  SRR4302543.4485278/1‑94 (MQ=60)
                                                                             GGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACA                  >  SRR4302543.4485280/1‑94 (MQ=60)
                                                                              GTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAA                 >  SRR4302543.2185697/1‑94 (MQ=60)
                                                                                TCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAA               >  SRR4302543.4329424/1‑94 (MQ=60)
                                                                                TCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAA               >  SRR4302543.4325401/1‑94 (MQ=60)
                                                                                TCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAA               >  SRR4302543.6885292/1‑94 (MQ=60)
                                                                                 CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              <  SRR4302543.6778368/94‑1 (MQ=60)
                                                                                 CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              >  SRR4302543.2625156/1‑94 (MQ=60)
                                                                                 CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              >  SRR4302543.1442401/1‑94 (MQ=60)
                                                                                 CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              <  SRR4302543.1835725/94‑1 (MQ=60)
                                                                                 CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              <  SRR4302543.500669/94‑1 (MQ=60)
                                                                                 CGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              <  SRR4302543.6292768/94‑1 (MQ=60)
                                                                                 CGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAT              >  SRR4302543.4671595/1‑94 (MQ=60)
                                                                                  GAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATC             <  SRR4302543.6760411/94‑1 (MQ=60)
                                                                                  GAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATC             >  SRR4302543.5836716/1‑94 (MQ=60)
                                                                                   AGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCC            >  SRR4302543.4212802/1‑94 (MQ=60)
                                                                                   AGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCC            <  SRR4302543.5053938/94‑1 (MQ=60)
                                                                                    GTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCG           <  SRR4302543.1774408/94‑1 (MQ=60)
                                                                                    GTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCG           >  SRR4302543.4883315/1‑94 (MQ=60)
                                                                                    GTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCG           <  SRR4302543.2888353/94‑1 (MQ=60)
                                                                                     TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG          >  SRR4302543.7146278/1‑94 (MQ=60)
                                                                                     TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG          <  SRR4302543.6124227/94‑1 (MQ=60)
                                                                                      CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         >  SRR4302543.6523613/1‑94 (MQ=60)
                                                                                      CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         >  SRR4302543.930853/1‑94 (MQ=60)
                                                                                      CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         >  SRR4302543.1004158/1‑94 (MQ=60)
                                                                                      CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA         >  SRR4302543.6523614/1‑94 (MQ=60)
                                                                                       GCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAC        <  SRR4302543.3148811/94‑1 (MQ=60)
                                                                                       GCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAC        <  SRR4302543.2706083/94‑1 (MQ=60)
                                                                                       CCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAC        <  SRR4302543.6299757/94‑1 (MQ=60)
                                                                                        CAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACG       <  SRR4302543.5379825/94‑1 (MQ=60)
                                                                                         AGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGT      >  SRR4302543.7598938/1‑94 (MQ=60)
                                                                                          GGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTC     <  SRR4302543.1984357/94‑1 (MQ=60)
                                                                                            CGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCG   >  SRR4302543.4582604/1‑94 (MQ=60)
                                                                                             GCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATGTCACGCAGAGATATTCATCAACGACAAAATCCGGACGTCCGG  <  SRR4302543.3043187/94‑1 (MQ=60)
                                                                                             GCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  <  SRR4302543.3235953/94‑1 (MQ=60)
                                                                                             GCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  >  SRR4302543.7947864/1‑94 (MQ=60)
                                                                                             GCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  >  SRR4302543.4835049/1‑94 (MQ=60)
                                                                                             ACTTTAAACAGATAGCAATGATGATGATGCCAGAAGCAGCCATGGGCAAATATCACGCAGCGATATTCATCACCGACACAATCCGCACGTCCGG  >  SRR4302543.2769753/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCGG  >  NC_000913/2028615‑2028801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑