Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,078,049	A→G	F189L (TTC→CTC)	yeeA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,078,049	0	A	G	99.6%	706.4 / NA	221	F189L (TTC→CTC)	yeeA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (102/118); minor base C (0/1); total (102/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGG  >  NC_000913/2077960‑2078138

cgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                        <  1:1013659/93‑1 (MQ=255)

 gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCa                                                                                       <  2:656542/93‑1 (MQ=255)

   aaTCAGTCCTCGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     <  2:2536310/93‑1 (MQ=255)

   aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     <  2:1795029/93‑1 (MQ=255)

   aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     <  1:7385802/93‑1 (MQ=255)

    aTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAt                                                                                    >  1:7363683/1‑93 (MQ=255)

    aTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGCAAGTTCGGTGAGAGTGCAGAt                                                                                    >  1:7236976/1‑93 (MQ=255)

     tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGGAAGTTCGGTGAGAGTGCAGAtt                                                                                   >  2:423869/1‑93 (MQ=255)

       aGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGa                                                                                 <  2:2088792/93‑1 (MQ=255)

       aGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGa                                                                                 <  1:5792161/93‑1 (MQ=255)

        gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTACGTTCGGTGAGCGTGCAGATTGAt                                                                                >  2:5657604/1‑93 (MQ=255)

        gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                                >  1:5887917/1‑93 (MQ=255)

        gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                                >  2:375263/1‑93 (MQ=255)

         tCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATa                                                                               <  1:2445312/93‑1 (MQ=255)

          ccACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAg                                                                              >  2:6587713/1‑93 (MQ=255)

          ccACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAg                                                                              >  1:1884983/1‑93 (MQ=255)

           cACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGa                                                                             <  1:2613416/93‑1 (MQ=255)

            aCGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAc                                                                            >  1:1417277/1‑93 (MQ=255)

            aCGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAc                                                                            <  2:7769947/93‑1 (MQ=255)

             cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAcc                                                                           <  1:6414817/93‑1 (MQ=255)

                   ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATta                                                                     <  1:2952759/93‑1 (MQ=255)

                   ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATta                                                                     <  2:5651701/93‑1 (MQ=255)

                   ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATta                                                                     <  2:7397405/93‑1 (MQ=255)

                     cACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATtata                                                                   <  1:3839581/93‑1 (MQ=255)

                     cACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATtata                                                                   <  1:4052378/93‑1 (MQ=255)

                      aCGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAc                                                                  >  2:3956882/1‑93 (MQ=255)

                       cGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACt                                                                 <  1:5385499/93‑1 (MQ=255)

                       cGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACt                                                                 <  2:2793612/93‑1 (MQ=255)

                        ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTc                                                                <  2:2882562/93‑1 (MQ=255)

                        ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTc                                                                <  1:1605868/93‑1 (MQ=255)

                         gCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCg                                                               <  2:818862/93‑1 (MQ=255)

                          cATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCgg                                                              <  2:3414260/93‑1 (MQ=255)

                           aTCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGt                                                             <  1:6737226/93‑1 (MQ=255)

                            tCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTc                                                            <  1:5271680/93‑1 (MQ=255)

                             cGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCa                                                           <  2:522682/93‑1 (MQ=255)

                               gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGa                                                         >  2:5363191/1‑93 (MQ=255)

                               gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGa                                                         <  2:6490086/93‑1 (MQ=255)

                               gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGa                                                         >  1:7842303/1‑93 (MQ=255)

                               gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGa                                                         >  2:7523693/1‑93 (MQ=255)

                                tCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAc                                                        >  1:1092974/1‑93 (MQ=255)

                                 cAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACt                                                       >  2:2200079/1‑93 (MQ=255)

                                  aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                      <  1:4098162/93‑1 (MQ=255)

                                   ggAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACttt                                                     >  1:6518580/1‑93 (MQ=255)

                                   ggAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACttt                                                     >  2:5069157/1‑93 (MQ=255)

                                   ggAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACttt                                                     <  1:7742697/93‑1 (MQ=255)

                                   ggAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACttt                                                     >  1:863534/1‑93 (MQ=255)

                                     actttctGTAGATGGCTTTCCAGACGTGTGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTc                                                   <  2:2419862/91‑1 (MQ=255)

                                     aGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTc                                                   <  2:7302956/93‑1 (MQ=255)

                                      gTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCg                                                  <  2:1727374/93‑1 (MQ=255)

                                       tttttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGc                                                 <  2:1593747/93‑1 (MQ=255)

                                        ttttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGcc                                                >  2:1868566/1‑93 (MQ=255)

                                        ttttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGcc                                                <  1:4933731/93‑1 (MQ=255)

                                           tGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGt                                             <  2:131799/93‑1 (MQ=255)

                                            gTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTAAGACTTTTCGCCAGtt                                            >  1:5532007/1‑93 (MQ=255)

                                              aGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGa                                          >  1:2288166/1‑93 (MQ=255)

                                               gATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGaa                                         <  1:2529594/93‑1 (MQ=255)

                                               gATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGaa                                         >  2:6798714/1‑93 (MQ=255)

                                               gATGGCTTTCCAGACGTGGGCGTTCGAGCAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGaa                                         <  1:216576/93‑1 (MQ=255)

                                                 tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                       <  2:1838571/93‑1 (MQ=255)

                                                 tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                       <  1:1636844/93‑1 (MQ=255)

                                                 tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                       >  2:3548432/1‑93 (MQ=255)

                                                 tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                       <  2:2606819/93‑1 (MQ=255)

                                                 tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                       >  2:2923565/1‑93 (MQ=255)

                                                  ggCTTTCCAGGCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATgc                                      <  1:3782807/93‑1 (MQ=255)

                                                  ggCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATgc                                      >  2:4527417/1‑93 (MQ=255)

                                                      ttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATCCTCGGTCAGACTTTTCGCCAGTTGAATGCGCCa                                  <  1:754206/93‑1 (MQ=255)

                                                      ttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCa                                  <  2:7645423/93‑1 (MQ=255)

                                                      ttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGACAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCa                                  <  1:5105489/93‑1 (MQ=255)

                                                       tCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAg                                 <  2:5584818/93‑1 (MQ=255)

                                                       tCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAg                                 <  1:1280488/93‑1 (MQ=255)

                                                        ccAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGCGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGt                                >  2:1985535/1‑93 (MQ=255)

                                                         cAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTg                               >  1:3807279/1‑93 (MQ=255)

                                                          aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              >  2:5835467/1‑93 (MQ=255)

                                                          aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              >  2:3029256/1‑93 (MQ=255)

                                                           gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                             >  2:3808835/1‑93 (MQ=255)

                                                            aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                            >  2:4537284/1‑93 (MQ=255)

                                                            aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                            >  2:7050370/1‑93 (MQ=255)

                                                            aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                            >  2:5458738/1‑93 (MQ=255)

                                                             cGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATg                           >  2:1454151/1‑93 (MQ=255)

                                                              gTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGa                          <  1:3142015/93‑1 (MQ=255)

                                                               tGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGaa                         >  1:3435519/1‑93 (MQ=255)

                                                               tGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGaa                         >  1:5714262/1‑93 (MQ=255)

                                                               tGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGaa                         <  2:3104058/93‑1 (MQ=255)

                                                               tGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGaa                         <  1:3689267/93‑1 (MQ=255)

                                                                gggCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                        <  2:3559069/93‑1 (MQ=255)

                                                                gggCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                        <  1:166410/93‑1 (MQ=255)

                                                                  gCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAACCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc                      <  1:2114657/93‑1 (MQ=255)

                                                                   cGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGcc                     <  1:4687205/93‑1 (MQ=255)

                                                                    gTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGccc                    >  1:6827018/1‑93 (MQ=255)

                                                                     ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                   >  2:6121271/1‑93 (MQ=255)

                                                                     ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                   >  1:3055009/1‑93 (MQ=255)

                                                                     ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                   >  1:6718649/1‑93 (MQ=255)

                                                                     ttCGAGTAAGTTCGGCGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCACg                   >  1:1770610/1‑93 (MQ=255)

                                                                        gAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtg                >  2:6763830/1‑93 (MQ=255)

                                                                        gAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtg                >  2:6882404/1‑93 (MQ=255)

                                                                                tCGGTGAGAGTGCAGATGGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGa        <  2:906943/93‑1 (MQ=255)

                                                                                  ggTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATa      >  2:6955015/1‑93 (MQ=255)

                                                                                  ggTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCACCAGTGGATGAACGCCCGTTGTGGCCAGATa      <  1:7579399/93‑1 (MQ=255)

                                                                                    tGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAcc    <  2:3667038/93‑1 (MQ=255)

                                                                                    tGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAcc    <  1:2804680/93‑1 (MQ=255)

CGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGG  >  NC_000913/2077960‑2078138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077956‑2078142

GGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302543.2050917/94‑1 (MQ=60)

CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302543.5291617/94‑1 (MQ=60)

CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302543.953523/94‑1 (MQ=60)

CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302543.631592/94‑1 (MQ=60)

CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302543.4008418/94‑1 (MQ=60)

 GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302543.1913740/94‑1 (MQ=60)

 GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302543.2821654/94‑1 (MQ=60)

 GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302543.2475284/94‑1 (MQ=60)

 GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              >  SRR4302543.1238260/1‑94 (MQ=60)

 GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302543.3076113/94‑1 (MQ=60)

 GGGCGCAATCAGTCCACGCATGTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302543.2083063/94‑1 (MQ=60)

  GGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTG                                                                                             <  SRR4302543.690341/94‑1 (MQ=60)

    CGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCA                                                                                           <  SRR4302543.1022629/94‑1 (MQ=60)

     GCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAG                                                                                          >  SRR4302543.6435658/1‑94 (MQ=60)

     GCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAG                                                                                          <  SRR4302543.2174951/94‑1 (MQ=60)

     GCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAG                                                                                          <  SRR4302543.5788479/94‑1 (MQ=60)

       AATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAT                                                                                        <  SRR4302543.1782347/94‑1 (MQ=60)

       AATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAT                                                                                        <  SRR4302543.7456774/94‑1 (MQ=60)

       AATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAT                                                                                        >  SRR4302543.7434492/1‑94 (MQ=60)

       AATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGCAAGTTCGGTGAGAGTGCAGAT                                                                                        >  SRR4302543.7307045/1‑94 (MQ=60)

        ATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATT                                                                                       >  SRR4302543.3104397/1‑94 (MQ=60)

           AGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAT                                                                                    <  SRR4302543.5844854/94‑1 (MQ=60)

           AGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAT                                                                                    >  SRR4302543.5941590/1‑94 (MQ=60)

            GTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATA                                                                                   >  SRR4302543.6800355/1‑94 (MQ=60)

             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  >  SRR4302543.1901923/1‑94 (MQ=60)

             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  <  SRR4302543.3015417/94‑1 (MQ=60)

             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  <  SRR4302543.2467350/94‑1 (MQ=60)

              CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGA                                                                                 >  SRR4302543.4704751/1‑94 (MQ=60)

               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                >  SRR4302543.4117812/1‑94 (MQ=60)

               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                >  SRR4302543.59952/1‑94 (MQ=60)

               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                <  SRR4302543.2637123/94‑1 (MQ=60)

               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                >  SRR4302543.1430106/1‑94 (MQ=60)

               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                >  SRR4302543.7228229/1‑94 (MQ=60)

                 CGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCC                                                                              <  SRR4302543.6475242/94‑1 (MQ=60)

                  GCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCG                                                                             >  SRR4302543.1668122/1‑94 (MQ=60)

                  GCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCG                                                                             <  SRR4302543.3746831/94‑1 (MQ=60)

                    ATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAT                                                                           >  SRR4302543.1565550/1‑94 (MQ=60)

                       TTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTAT                                                                        <  SRR4302543.2979570/94‑1 (MQ=60)

                       TTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTAT                                                                        <  SRR4302543.6890473/94‑1 (MQ=60)

                         CACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAC                                                                      <  SRR4302543.3873473/94‑1 (MQ=60)

                         CACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAC                                                                      <  SRR4302543.4088646/94‑1 (MQ=60)

                           CGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTC                                                                    >  SRR4302543.5270340/1‑94 (MQ=60)

                           CGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTC                                                                    <  SRR4302543.5434394/94‑1 (MQ=60)

                           CGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTC                                                                    <  SRR4302543.7727510/94‑1 (MQ=60)

                            GGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCG                                                                   <  SRR4302543.1620196/94‑1 (MQ=60)

                             GCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGG                                                                  >  SRR4302543.5486616/1‑94 (MQ=60)

                               ATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTC                                                                <  SRR4302543.6800858/94‑1 (MQ=60)

                               ATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTC                                                                >  SRR4302543.7637062/1‑94 (MQ=60)

                               ATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTC                                                                >  SRR4302543.7525132/1‑94 (MQ=60)

                               ATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTC                                                                >  SRR4302543.357522/1‑94 (MQ=60)

                                TCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCA                                                               <  SRR4302543.5319236/94‑1 (MQ=60)

                                 CGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAG                                                              <  SRR4302543.2694519/94‑1 (MQ=60)

                                 CGGTCAGGAGTTTTTGTAGACGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAG                                                              <  SRR4302543.5713404/94‑1 (MQ=60)

                                  GGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGA                                                             >  SRR4302543.7916228/1‑94 (MQ=60)

                                   GTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAC                                                            >  SRR4302543.5387992/1‑94 (MQ=60)

                                   GTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAC                                                            >  SRR4302543.1102801/1‑94 (MQ=60)

                                      AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         >  SRR4302543.871237/1‑94 (MQ=60)

                                      AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         >  SRR4302543.1358824/1‑94 (MQ=60)

                                      AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         >  SRR4302543.3171851/1‑94 (MQ=60)

                                      AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         <  SRR4302543.4134976/94‑1 (MQ=60)

                                      AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         >  SRR4302543.7892563/1‑94 (MQ=60)

                                      AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         >  SRR4302543.6579969/1‑94 (MQ=60)

                                       GGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTT                                                        <  SRR4302543.7815835/94‑1 (MQ=60)

                                            TTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCA                                                   <  SRR4302543.4978672/94‑1 (MQ=60)

                                             TTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAG                                                  <  SRR4302543.4854293/94‑1 (MQ=60)

                                               TGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTAAGACTTTTCGCCAGTT                                                >  SRR4302543.5582273/1‑94 (MQ=60)

                                                 TAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGA                                              <  SRR4302543.1996051/94‑1 (MQ=60)

                                                 TAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGA                                              >  SRR4302543.2309058/1‑94 (MQ=60)

                                                  AGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAA                                             >  SRR4302543.3865078/1‑94 (MQ=60)

                                                  AGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAA                                             >  SRR4302543.6476696/1‑94 (MQ=60)

                                                   GGTGGCTTTCCAGACGTTGGCGTTCGACTAAGTTCGGTGAGAATCCCGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAT                                            <  SRR4302543.2552845/94‑1 (MQ=60)

                                                   GATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAT                                            <  SRR4302543.2552610/94‑1 (MQ=60)

                                                   GATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAT                                            <  SRR4302543.105936/94‑1 (MQ=60)

                                                   GATGGCTTTCCAGACGTGGGCGTTCGAGCAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAT                                            <  SRR4302543.218442/94‑1 (MQ=60)

                                                    ATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATG                                           >  SRR4302543.5510826/1‑94 (MQ=60)

                                                    ATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATG                                           >  SRR4302543.3983471/1‑94 (MQ=60)

                                                    ATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATG                                           >  SRR4302543.2423566/1‑94 (MQ=60)

                                                    ATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATG                                           >  SRR4302543.7418257/1‑94 (MQ=60)

                                                     TGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGC                                          <  SRR4302543.1651450/94‑1 (MQ=60)

                                                      GGCTTTCCAGGCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCG                                         <  SRR4302543.3816132/94‑1 (MQ=60)

                                                      GGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCG                                         <  SRR4302543.3944391/94‑1 (MQ=60)

                                                      GGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCG                                         <  SRR4302543.3816135/94‑1 (MQ=60)

                                                          TTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATCCTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAG                                     <  SRR4302543.760879/94‑1 (MQ=60)

                                                          TTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAG                                     <  SRR4302543.4993718/94‑1 (MQ=60)

                                                          TTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGACAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAG                                     <  SRR4302543.5151689/94‑1 (MQ=60)

                                                           TCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGT                                    <  SRR4302543.1291901/94‑1 (MQ=60)

                                                            CCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTG                                   >  SRR4302543.3840847/1‑94 (MQ=60)

                                                            CCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTG                                   <  SRR4302543.5876734/94‑1 (MQ=60)

                                                            CCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTG                                   <  SRR4302543.829613/94‑1 (MQ=60)

                                                               GACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAT                                <  SRR4302543.1646860/94‑1 (MQ=60)

                                                                  GTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAA                             >  SRR4302543.5766399/1‑94 (MQ=60)

                                                                  GTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAA                             >  SRR4302543.3466270/1‑94 (MQ=60)

                                                                  GTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAA                             <  SRR4302543.3170276/94‑1 (MQ=60)

                                                                   TGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAC                            <  SRR4302543.3721691/94‑1 (MQ=60)

                                                                   TGGACGTTCGAGTAAGTTCGATGAGAGTGCAGACTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAC                            <  SRR4302543.6657581/94‑1 (MQ=60)

                                                                    GGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACG                           <  SRR4302543.167846/94‑1 (MQ=60)

                                                                    GGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACG                           <  SRR4302543.2239500/94‑1 (MQ=60)

                                                                     GGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGC                          >  SRR4302543.1559492/1‑94 (MQ=60)

                                                                      GCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAACCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCC                         <  SRR4302543.2133588/94‑1 (MQ=60)

                                                                       CGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC                        >  SRR4302543.6869624/1‑94 (MQ=60)

                                                                       CGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC                        <  SRR4302543.4729976/94‑1 (MQ=60)

                                                                       CGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC                        >  SRR4302543.6891467/1‑94 (MQ=60)

                                                                        GTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCG                       >  SRR4302543.6782148/1‑94 (MQ=60)

                                                                        GTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCG                       >  SRR4302543.3082518/1‑94 (MQ=60)

                                                                        GTTCGAGTAAGTTCGGCGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCACG                       >  SRR4302543.1786441/1‑94 (MQ=60)

                                                                            GAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGT                   >  SRR4302543.822538/1‑94 (MQ=60)

                                                                             AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  <  SRR4302543.4805662/94‑1 (MQ=60)

                                                                                 AGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCA              >  SRR4302543.2925899/1‑94 (MQ=60)

                                                                                 AGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCA              >  SRR4302543.2925900/1‑94 (MQ=60)

                                                                                 AGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCA              >  SRR4302543.3477728/1‑94 (MQ=60)

                                                                                  GTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAG             <  SRR4302543.126729/94‑1 (MQ=60)

                                                                                    TCGGTGAGAGTGCAGATTGATAGACCCGCTTATCCTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAT           <  SRR4302543.3544873/94‑1 (MQ=60)

                                                                                    TCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAT           <  SRR4302543.7446496/94‑1 (MQ=60)

                                                                                     CGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATA          <  SRR4302543.2524930/94‑1 (MQ=60)

                                                                                      GGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAC         >  SRR4302543.1847822/1‑94 (MQ=60)

                                                                                      GGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAC         >  SRR4302543.1847823/1‑94 (MQ=60)

                                                                                      GGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCACCAGTGGATGAACGCCCGTTGTGGCCAGATAC         <  SRR4302543.7651459/94‑1 (MQ=60)

                                                                                        TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       <  SRR4302543.2397191/94‑1 (MQ=60)

                                                                                        TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       <  SRR4302543.2830338/94‑1 (MQ=60)

                                                                                           GAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTA    >  SRR4302543.4504198/1‑94 (MQ=60)

                                                                                            AGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAA   <  SRR4302543.178078/94‑1 (MQ=60)

                                                                                            AGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAA   >  SRR4302543.932963/1‑94 (MQ=60)

                                                                                            AGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGCAA   >  SRR4302543.985336/1‑94 (MQ=60)

                                                                                             GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  SRR4302543.2353020/1‑94 (MQ=60)

                                                                                             GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTAGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  SRR4302543.7636990/1‑94 (MQ=60)

CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077956‑2078142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`