Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,116,015 A→G F138L (TTT→CTT)  wcaK ← putative galactokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,116,0150AG99.6% 695.9 / ‑5.3 221F138L (TTT→CTT) wcaKputative galactokinase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (120/100);  total (121/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 226 total aligned reads displayed.

GGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGA  >  NC_000913/2115925‑2116104
                                                                                          |                                                                                         
ggTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCt                                                                                         >  1:3397663/1‑93 (MQ=255)
 gTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTg                                                                                        >  2:2899306/1‑93 (MQ=255)
  ttAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGc                                                                                       >  2:563089/1‑93 (MQ=255)
  atAAATTGCTCAACATAAAACGGGCCAACACTGTAACCAATCATAAACAGCAGCTTATACGCCATAAACGTGCAAAGTGCATGTTAAAGCTGc                                                                                       <  1:7038886/92‑1 (MQ=255)
     aaTTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGc                                                                                    >  2:3245073/1‑93 (MQ=255)
      aTTGCTCATCCTGAAACGGGCCAACACTGTGACTAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCa                                                                                   <  1:7630148/93‑1 (MQ=255)
       ttGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCACAGTGCATGTTCAAGCTGCGGCAc                                                                                  >  2:473468/1‑93 (MQ=255)
       ttGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCAc                                                                                  <  2:3099910/93‑1 (MQ=255)
        tGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACg                                                                                 >  1:703678/1‑93 (MQ=255)
          cTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGcc                                                                               >  1:6735297/1‑93 (MQ=255)
           tCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGAGGAACGCCa                                                                              >  2:822404/1‑93 (MQ=255)
            cATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCAt                                                                             >  2:4986151/1‑93 (MQ=255)
            cATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCAt                                                                             <  2:2269239/93‑1 (MQ=255)
               ccTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATaga                                                                          <  2:895640/93‑1 (MQ=255)
               ccTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATaga                                                                          <  2:5934413/93‑1 (MQ=255)
                cTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATagag                                                                         >  2:1213204/1‑93 (MQ=255)
                cTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATagag                                                                         >  1:2557261/1‑93 (MQ=255)
                 tGGAACGTGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAgaga                                                                        <  1:58201/93‑1 (MQ=255)
                 tGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAgaga                                                                        >  2:5532769/1‑93 (MQ=255)
                 tGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAgaga                                                                        <  1:2248748/93‑1 (MQ=255)
                 tGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAgaga                                                                        <  2:2033661/93‑1 (MQ=255)
                   gAACGGTCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATc                                                                      <  2:559226/93‑1 (MQ=255)
                   gAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATc                                                                      >  2:3774321/1‑93 (MQ=255)
                   gAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATc                                                                      <  2:3675565/93‑1 (MQ=255)
                    aaCGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCg                                                                     <  1:2821533/93‑1 (MQ=255)
                       gggCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACa                                                                  >  1:4637480/1‑93 (MQ=255)
                          gcAACACTGTGACGAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACaaaa                                                               <  1:4809285/92‑1 (MQ=255)
                            aaCACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACaaaaaa                                                             <  1:6488082/93‑1 (MQ=255)
                            aaCACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACaaaaaa                                                             >  1:4759766/1‑93 (MQ=255)
                            aaCACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACaaaaaa                                                             <  1:7404204/93‑1 (MQ=255)
                              cacTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACg                                                           <  2:6360646/93‑1 (MQ=255)
                               acTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGa                                                          <  2:6929244/93‑1 (MQ=255)
                               acTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGa                                                          >  2:60170/1‑93 (MQ=255)
                                cTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGAt                                                         <  1:7104944/93‑1 (MQ=255)
                                cTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGAt                                                         <  1:365144/93‑1 (MQ=255)
                                cTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGAt                                                         >  2:6872679/1‑93 (MQ=255)
                                 tgtgACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATc                                                        >  1:5985632/1‑93 (MQ=255)
                                 tgtgACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATc                                                        >  2:6389830/1‑93 (MQ=255)
                                  gtgACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATcc                                                       <  2:2952372/93‑1 (MQ=255)
                                  gtgACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATcc                                                       <  1:2971547/93‑1 (MQ=255)
                                  gtgACCAATCATAAACAGCGGCTTTTGCGCCATAAACGTGCAAACTTCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATcc                                                       <  1:7442188/93‑1 (MQ=255)
                                   tgACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATccg                                                      >  1:2597890/1‑93 (MQ=255)
                                    gACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATccgc                                                     >  2:3842226/1‑93 (MQ=255)
                                     aCCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATccgcc                                                    <  2:4266837/93‑1 (MQ=255)
                                       cAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATccgccgc                                                  >  1:3241428/1‑92 (MQ=255)
                                        aaTCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc                                                 >  1:7022298/1‑93 (MQ=255)
                                        aaTCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc                                                 >  1:5325199/1‑93 (MQ=255)
                                         aTCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAcc                                                >  2:7078130/1‑93 (MQ=255)
                                           cATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTg                                              <  2:7521407/93‑1 (MQ=255)
                                           cATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTg                                              <  2:733766/93‑1 (MQ=255)
                                           cATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTg                                              <  2:1445985/93‑1 (MQ=255)
                                             tAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGa                                            >  2:2519808/1‑93 (MQ=255)
                                              aaaCAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGAt                                           >  2:5403345/1‑93 (MQ=255)
                                                aCAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATaa                                         <  1:3194395/93‑1 (MQ=255)
                                                aCAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATaa                                         >  2:5276990/1‑93 (MQ=255)
                                                aCAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATaa                                         <  1:5436469/93‑1 (MQ=255)
                                                 cAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAt                                        <  1:2358828/93‑1 (MQ=255)
                                                 cAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAt                                        >  1:3761495/1‑93 (MQ=255)
                                                 cAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAt                                        >  1:7515472/1‑93 (MQ=255)
                                                  aGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATg                                       <  1:4037571/93‑1 (MQ=255)
                                                   gCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATgg                                      <  2:481362/93‑1 (MQ=255)
                                                   gCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATgg                                      >  1:1163833/1‑93 (MQ=255)
                                                    cGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGc                                     <  1:1459712/93‑1 (MQ=255)
                                                      gCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGcgt                                   <  1:6796603/93‑1 (MQ=255)
                                                      gCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGcgt                                   >  2:6156890/1‑93 (MQ=255)
                                                      gCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGcgt                                   >  2:4424157/1‑93 (MQ=255)
                                                       cTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGcgtc                                  >  1:6359904/1‑93 (MQ=255)
                                                         ttttCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGcgtcgt                                >  1:1655336/1‑93 (MQ=255)
                                                             cGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAAcc                            <  2:1610638/93‑1 (MQ=255)
                                                              gCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCt                           <  2:3627001/93‑1 (MQ=255)
                                                              gCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCt                           >  1:7209721/1‑93 (MQ=255)
                                                                cATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGa                         <  1:5789109/93‑1 (MQ=255)
                                                                 aTAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAc                        <  2:1958520/93‑1 (MQ=255)
                                                                 aTAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAc                        >  2:6225191/1‑93 (MQ=255)
                                                                  tAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAca                       >  1:2335235/1‑93 (MQ=255)
                                                                  tAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAca                       >  2:4078620/1‑93 (MQ=255)
                                                                  tAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAca                       >  2:6350239/1‑93 (MQ=255)
                                                                  tAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAca                       <  2:1697265/93‑1 (MQ=255)
                                                                     aCGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAcagca                    <  2:7736834/93‑1 (MQ=255)
                                                                     aCGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAcagca                    <  2:6632783/93‑1 (MQ=255)
                                                                     aCGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAcagca                    <  1:3685005/93‑1 (MQ=255)
                                                                     aCGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAcagca                    <  1:4917404/93‑1 (MQ=255)
                                                                     aCGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAcagca                    <  2:5191433/93‑1 (MQ=255)
                                                                      cGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAcagcag                   <  2:5804645/93‑1 (MQ=255)
                                                                       gTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAgg                  >  1:6318209/1‑93 (MQ=255)
                                                                           aaaGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCa              <  1:2052612/93‑1 (MQ=255)
                                                                           aaaGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCa              >  1:5366528/1‑93 (MQ=255)
                                                                           aaaGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCa              >  1:728454/1‑93 (MQ=255)
                                                                             aGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACg            >  1:7806791/1‑93 (MQ=255)
                                                                             aGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACg            <  1:5106639/93‑1 (MQ=255)
                                                                             aGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACg            >  2:876725/1‑93 (MQ=255)
                                                                               tGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGaa          <  2:7484979/93‑1 (MQ=255)
                                                                               ggCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGaa          <  1:4923885/92‑1 (MQ=255)
                                                                                  aTGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTc       <  2:5323803/93‑1 (MQ=255)
                                                                                   tGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg      >  2:4005397/1‑93 (MQ=255)
                                                                                   tGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg      >  1:4488704/1‑93 (MQ=255)
                                                                                    gTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCgg     >  1:3347376/1‑93 (MQ=255)
                                                                                    gTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCgg     >  2:6279656/1‑93 (MQ=255)
                                                                                    gTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCgg     >  1:4155951/1‑93 (MQ=255)
                                                                                       cAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGa  >  1:1405189/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGA  >  NC_000913/2115925‑2116104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATC  >  NC_000913/2115922‑2116107
                                                                                             |                                                                                            
GTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAG                                                                                              <  SRR4302543.1740029/94‑1 (MQ=60)
  TGGTTAAATTGCTCATCCTGGAGCGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTGCGCCATAAACGTGCAAAGTGCATGTTCAAGCT                                                                                            <  SRR4302543.1678115/94‑1 (MQ=60)
  TGGTTAAATTGCTCATCCTGGAACGGTCCAACGCTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCT                                                                                            <  SRR4302543.210756/94‑1 (MQ=60)
  TGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCT                                                                                            <  SRR4302543.2146657/94‑1 (MQ=60)
  TGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCT                                                                                            >  SRR4302543.7583793/1‑94 (MQ=60)
  TGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCT                                                                                            >  SRR4302543.3428112/1‑94 (MQ=60)
  TGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCT                                                                                            <  SRR4302543.5265892/94‑1 (MQ=60)
   GGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTG                                                                                           >  SRR4302543.5394764/1‑94 (MQ=60)
   GGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTG                                                                                           >  SRR4302543.2358719/1‑94 (MQ=60)
     ataaattgctcaacataaAACGGGCCAACACTGTAACCAATCATAAACAGCAGCTTATACGCCATAAACGTGCAAAGTGCATGTTAAAGCTGCG                                                                                         <  SRR4302543.7106775/76‑1 (MQ=59)
     TTAAATTGCTCATCCTGAACCGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCG                                                                                         <  SRR4302543.7106774/94‑1 (MQ=60)
      TAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGG                                                                                        <  SRR4302543.6667008/94‑1 (MQ=60)
        AATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCA                                                                                      <  SRR4302543.7316235/94‑1 (MQ=60)
         ATTGCTCATCCTGAAACGGGCCAACACTGTGACTAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCAC                                                                                     <  SRR4302543.7702553/94‑1 (MQ=60)
          TTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACG                                                                                    >  SRR4302543.710092/1‑94 (MQ=60)
          TGGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACG                                                                                    <  SRR4302543.413125/94‑1 (MQ=60)
            GCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCC                                                                                  >  SRR4302543.6798920/1‑94 (MQ=60)
             CTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCA                                                                                 >  SRR4302543.4187721/1‑94 (MQ=60)
              TCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCAT                                                                                <  SRR4302543.3194861/94‑1 (MQ=60)
              TCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCAT                                                                                >  SRR4302543.4263430/1‑94 (MQ=60)
               CATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATA                                                                               >  SRR4302543.3643927/1‑94 (MQ=60)
               CATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATA                                                                               >  SRR4302543.1598202/1‑94 (MQ=60)
                ATCCTGGAACGGGTCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAG                                                                              <  SRR4302543.256647/94‑1 (MQ=60)
                ATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAG                                                                              <  SRR4302543.2006921/94‑1 (MQ=60)
                ATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAG                                                                              >  SRR4302543.3891790/1‑94 (MQ=60)
                 TCCTGTAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGA                                                                             <  SRR4302543.5061258/94‑1 (MQ=60)
                  CCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAG                                                                            >  SRR4302543.2580637/1‑94 (MQ=60)
                   CTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGA                                                                           >  SRR4302543.7396913/1‑94 (MQ=60)
                    TGGAACGTGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGAT                                                                          <  SRR4302543.58714/94‑1 (MQ=60)
                    TGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGAT                                                                          <  SRR4302543.2269169/94‑1 (MQ=60)
                     GGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATC                                                                         <  SRR4302543.2704593/94‑1 (MQ=60)
                       AACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGA                                                                       <  SRR4302543.2847349/94‑1 (MQ=60)
                         CGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACA                                                                     >  SRR4302543.4679654/1‑94 (MQ=60)
                           GGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAA                                                                   >  SRR4302543.1006845/1‑94 (MQ=60)
                            GCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAA                                                                  <  SRR4302543.529273/94‑1 (MQ=60)
                             GCAACACTGTGACGAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAA                                                                 <  SRR4302543.4853208/94‑1 (MQ=60)
                             CCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAA                                                                 >  SRR4302543.6828618/1‑94 (MQ=60)
                             CCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAA                                                                 <  SRR4302543.4853206/94‑1 (MQ=60)
                              CAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAA                                                                <  SRR4302543.458960/94‑1 (MQ=60)
                              CAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAA                                                                >  SRR4302543.4803255/1‑94 (MQ=60)
                               AACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAAC                                                               <  SRR4302543.6549204/94‑1 (MQ=60)
                               AACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAAC                                                               <  SRR4302543.2082132/94‑1 (MQ=60)
                               AACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAAC                                                               <  SRR4302543.7475272/94‑1 (MQ=60)
                                ACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACG                                                              >  SRR4302543.7249400/1‑94 (MQ=60)
                                ACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACG                                                              >  SRR4302543.1886461/1‑94 (MQ=60)
                                  ACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGAT                                                            <  SRR4302543.7092106/94‑1 (MQ=60)
                                   CTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCACAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATC                                                           >  SRR4302543.2280902/1‑94 (MQ=60)
                                   CTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATC                                                           <  SRR4302543.368524/94‑1 (MQ=60)
                                   CTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATC                                                           >  SRR4302543.6040482/1‑94 (MQ=60)
                                   CTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATC                                                           <  SRR4302543.7173957/94‑1 (MQ=60)
                                    TGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCGGCGGCACGCCATAGAGATCGACAAAAAACGATCC                                                          <  SRR4302543.2459553/94‑1 (MQ=60)
                                     GTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCG                                                         <  SRR4302543.7777419/94‑1 (MQ=60)
                                     GTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCG                                                         <  SRR4302543.2998531/94‑1 (MQ=60)
                                     GTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCG                                                         >  SRR4302543.2621490/1‑94 (MQ=60)
                                     GTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCG                                                         <  SRR4302543.6389303/94‑1 (MQ=60)
                                     GTGACCAATCATAAACAGCGGCTTTTGCGCCATAAACGTGCAAACTTCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCG                                                         <  SRR4302543.7513519/94‑1 (MQ=60)
                                        ACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCG                                                      <  SRR4302543.2586431/94‑1 (MQ=60)
                                         CCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGC                                                     >  SRR4302543.3270748/1‑94 (MQ=60)
                                         CCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGA                                                     >  SRR4302543.3270747/1‑94 (MQ=60)
                                         CCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGA                                                     >  SRR4302543.7161628/1‑94 (MQ=60)
                                          CAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC                                                    >  SRR4302543.7089573/1‑94 (MQ=60)
                                          CAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC                                                    >  SRR4302543.5373357/1‑94 (MQ=60)
                                           AATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACC                                                   >  SRR4302543.702077/1‑94 (MQ=60)
                                            ATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCT                                                  >  SRR4302543.6391847/1‑94 (MQ=60)
                                             TCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAGACGATACGCCGACCTG                                                 >  SRR4302543.4967565/1‑94 (MQ=60)
                                               ATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGA                                               >  SRR4302543.3389590/1‑94 (MQ=60)
                                               ATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGA                                               >  SRR4302543.6990357/1‑94 (MQ=60)
                                               ATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGACATAGAGATCGACAAAAAACGATCCGCCGACCTGGA                                               <  SRR4302543.5811525/94‑1 (MQ=60)
                                                   ACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAT                                           <  SRR4302543.3223194/94‑1 (MQ=60)
                                                   ACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAT                                           >  SRR4302543.7587236/1‑94 (MQ=60)
                                                   ACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAT                                           <  SRR4302543.1452706/94‑1 (MQ=60)
                                                   ACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAT                                           >  SRR4302543.3794600/1‑94 (MQ=60)
                                                   ACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAAT                                           <  SRR4302543.5485844/94‑1 (MQ=60)
                                                    CAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATG                                          <  SRR4302543.2380217/94‑1 (MQ=60)
                                                     AGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGG                                         >  SRR4302543.1174427/1‑94 (MQ=60)
                                                     AGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGG                                         <  SRR4302543.4073705/94‑1 (MQ=60)
                                                     AGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAAAGATCAGCCGACCTGGCTAATGG                                         >  SRR4302543.3548466/1‑94 (MQ=60)
                                                     AGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCCGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGG                                         >  SRR4302543.5036712/1‑94 (MQ=60)
                                                       CGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCG                                       <  SRR4302543.1472876/94‑1 (MQ=60)
                                                         GCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTC                                     <  SRR4302543.6860786/94‑1 (MQ=60)
                                                         GCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTC                                     >  SRR4302543.7611040/1‑94 (MQ=60)
                                                         GCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTC                                     >  SRR4302543.6419704/1‑94 (MQ=60)
                                                         GCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTC                                     <  SRR4302543.749007/94‑1 (MQ=60)
                                                          CTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCG                                    <  SRR4302543.4841703/94‑1 (MQ=60)
                                                           TTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGT                                   >  SRR4302543.1670156/1‑94 (MQ=60)
                                                                CGCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCT                              >  SRR4302543.7279611/1‑94 (MQ=60)
                                                                 GCCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTG                             >  SRR4302543.3693895/1‑94 (MQ=60)
                                                                  CCATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGA                            >  SRR4302543.3141668/1‑94 (MQ=60)
                                                                   CATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAC                           <  SRR4302543.5841784/94‑1 (MQ=60)
                                                                   CATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAC                           <  SRR4302543.60700/94‑1 (MQ=60)
                                                                    ATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACA                          >  SRR4302543.2356425/1‑94 (MQ=60)
                                                                    ATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACA                          >  SRR4302543.6441530/1‑94 (MQ=60)
                                                                    ATAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACA                          >  SRR4302543.2926837/1‑94 (MQ=60)
                                                                     TAAACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAG                         <  SRR4302543.5888021/94‑1 (MQ=60)
                                                                        ACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAG                      <  SRR4302543.4962206/94‑1 (MQ=60)
                                                                        ACGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAG                      <  SRR4302543.3717385/94‑1 (MQ=60)
                                                                         CGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGG                     >  SRR4302543.5844253/1‑94 (MQ=60)
                                                                         CGTGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGG                     >  SRR4302543.6377630/1‑94 (MQ=60)
                                                                           TGCAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCG                   <  SRR4302543.2321341/94‑1 (MQ=60)
                                                                             CAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCA                 >  SRR4302543.734998/1‑94 (MQ=60)
                                                                             CAAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCA                 >  SRR4302543.5415110/1‑94 (MQ=60)
                                                                              AAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCAC                <  SRR4302543.5784858/94‑1 (MQ=60)
                                                                              AAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCAC                >  SRR4302543.548283/1‑94 (MQ=60)
                                                                              AAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCAC                <  SRR4302543.2070986/94‑1 (MQ=60)
                                                                              AAAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCAC                >  SRR4302543.7276188/1‑94 (MQ=60)
                                                                               AAGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACG               >  SRR4302543.7880380/1‑94 (MQ=60)
                                                                                AGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGA              <  SRR4302543.5152850/94‑1 (MQ=60)
                                                                                AGTGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGA              >  SRR4302543.378250/1‑94 (MQ=60)
                                                                                  TGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAG            <  SRR4302543.3371531/94‑1 (MQ=60)
                                                                                  GGCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAG            <  SRR4302543.4968744/94‑1 (MQ=60)
                                                                                   GCATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGT           <  SRR4302543.4786896/94‑1 (MQ=60)
                                                                                     ATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCG         >  SRR4302543.799976/1‑94 (MQ=60)
                                                                                     ATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCG         <  SRR4302543.4915682/94‑1 (MQ=60)
                                                                                     ATGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCG         >  SRR4302543.4529478/1‑94 (MQ=60)
                                                                                      TGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGG        >  SRR4302543.4193532/1‑94 (MQ=60)
                                                                                      TGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGG        >  SRR4302543.3377515/1‑94 (MQ=60)
                                                                                      TGTTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGG        <  SRR4302543.1325310/94‑1 (MQ=60)
                                                                                        TTCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTG      <  SRR4302543.7549707/94‑1 (MQ=60)
                                                                                         TCAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGA     >  SRR4302543.1417877/1‑94 (MQ=60)
                                                                                          CAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGCAGTCGGTGAA    >  SRR4302543.759052/1‑94 (MQ=60)
                                                                                          CAAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAA    >  SRR4302543.1837374/1‑94 (MQ=60)
                                                                                           AAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCTGAGAAT   >  SRR4302543.4529620/1‑94 (MQ=60)
                                                                                           AAGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAAC   >  SRR4302543.1098382/1‑94 (MQ=60)
                                                                                            AGCTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATC  <  SRR4302543.710881/94‑1 (MQ=60)
                                                                                            AGCTGCGGCACGACATAGAGATCGACAAAAAACGATCCGCCGCCCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATC  <  SRR4302543.2686045/94‑1 (MQ=60)
                                                                                             |                                                                                            
GTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATC  >  NC_000913/2115922‑2116107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑