Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,161,130	T→C	V78A (GTC→GCC)	baeS →	BaeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,161,130	0	T	C	99.6%	717.9 / ‑5.1	227	V78A (GTC→GCC)	baeS	BaeS
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (114/112); total (114/113)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

Only 100 of 231 total aligned reads displayed.

AACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCT  >  NC_000913/2161040‑2161219

aaCAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCt                                                                                         <  2:2233691/93‑1 (MQ=255)

 aCAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCtt                                                                                        >  2:1007465/1‑93 (MQ=255)

 aCAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCtt                                                                                        <  2:6647554/93‑1 (MQ=255)

  cAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCttt                                                                                       <  1:1666139/93‑1 (MQ=255)

   aGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTc                                                                                      <  2:7331492/93‑1 (MQ=255)

    gCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCa                                                                                     >  2:4694782/1‑93 (MQ=255)

       aTTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGAt                                                                                  >  1:4296638/1‑93 (MQ=255)

       aTTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGAt                                                                                  >  2:3178395/1‑93 (MQ=255)

        ttACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATc                                                                                 <  1:1145569/93‑1 (MQ=255)

        ttACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATc                                                                                 <  2:3202590/93‑1 (MQ=255)

         tACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATcc                                                                                >  1:4468189/1‑93 (MQ=255)

          aCAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCt                                                                               <  2:4972340/93‑1 (MQ=255)

           cAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTg                                                                              >  1:3644159/1‑93 (MQ=255)

              cTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGt                                                                           <  1:88538/93‑1 (MQ=255)

               tGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGtt                                                                          <  1:7018847/93‑1 (MQ=255)

               tGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGtt                                                                          >  1:5605780/1‑93 (MQ=255)

               tGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGATTTGCCTTTCAGATCCTGCGtt                                                                          >  2:2105500/1‑93 (MQ=255)

                gTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTc                                                                         >  1:5512453/1‑93 (MQ=255)

                gTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTc                                                                         >  2:1602627/1‑93 (MQ=255)

                gTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTc                                                                         >  1:7560680/1‑93 (MQ=255)

                gTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTc                                                                         >  1:1178437/1‑93 (MQ=255)

                gTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTc                                                                         <  2:7372993/93‑1 (MQ=255)

                 ttAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCa                                                                        <  1:3027692/93‑1 (MQ=255)

                 ttAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCa                                                                        <  1:6133842/93‑1 (MQ=255)

                  tAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCAt                                                                       <  2:5605770/93‑1 (MQ=255)

                    aGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCAttt                                                                     <  2:5121869/93‑1 (MQ=255)

                       gATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGaa                                                                  >  1:3731120/1‑93 (MQ=255)

                       gATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGaa                                                                  >  1:2888735/1‑93 (MQ=255)

                         tGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAaca                                                                <  2:2287354/93‑1 (MQ=255)

                          gcgcTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAacac                                                               <  2:7525930/93‑1 (MQ=255)

                            gcTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGa                                                             >  2:3314670/1‑93 (MQ=255)

                             cTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGAt                                                            <  2:6219736/93‑1 (MQ=255)

                             cTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGAt                                                            <  1:7827869/93‑1 (MQ=255)

                              ttGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATa                                                           >  1:81/1‑93 (MQ=255)

                               tGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATaa                                                          <  2:4190634/93‑1 (MQ=255)

                                 gCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAAtt                                                        <  1:1583469/93‑1 (MQ=255)

                                  cGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTc                                                       <  1:5971201/93‑1 (MQ=255)

                                  cGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTc                                                       <  2:589650/93‑1 (MQ=255)

                                   gAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCg                                                      >  2:6094081/1‑93 (MQ=255)

                                   gAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCg                                                      >  2:7905135/1‑93 (MQ=255)

                                    aGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCgg                                                     >  2:3462405/1‑93 (MQ=255)

                                     gCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGa                                                    <  1:4570797/93‑1 (MQ=255)

                                       aGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAg                                                  <  1:3116931/93‑1 (MQ=255)

                                        gTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGa                                                 <  2:367104/93‑1 (MQ=255)

                                        gTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGa                                                 <  2:2499799/93‑1 (MQ=255)

                                         tATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGAt                                                >  2:2357942/1‑93 (MQ=255)

                                         tATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGAt                                                >  2:6764670/1‑93 (MQ=255)

                                          aTGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATa                                               >  1:7228889/1‑93 (MQ=255)

                                          aTGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATa                                               <  2:4416477/93‑1 (MQ=255)

                                           tGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGCACACGATAATTCGGAAGATaa                                              <  1:1332619/93‑1 (MQ=255)

                                           tGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATaa                                              >  2:5819196/1‑93 (MQ=255)

                                           tGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATaa                                              >  2:3562670/1‑93 (MQ=255)

                                           tGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATaa                                              <  1:6367329/93‑1 (MQ=255)

                                            gcgcAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATaaa                                             <  2:103136/93‑1 (MQ=255)

                                            gcgcAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATaaa                                             <  1:2270536/93‑1 (MQ=255)

                                             cgcAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAc                                            <  1:97096/93‑1 (MQ=255)

                                               cagcaTGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAccc                                          <  1:1454682/93‑1 (MQ=255)

                                               cagcaTGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAccc                                          <  2:6501577/93‑1 (MQ=255)

                                               cagcaTGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAccc                                          >  1:4197291/1‑93 (MQ=255)

                                                agcaTGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAcccg                                         <  1:739333/93‑1 (MQ=255)

                                                 gcaTGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAcccgg                                        >  2:7851344/1‑93 (MQ=255)

                                                   aTGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAcccggcc                                      >  1:4271656/1‑93 (MQ=255)

                                                    tGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAcccggccc                                     >  2:4932046/1‑93 (MQ=255)

                                                      gCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCTTGCGTTCATTTGAACACGATAATTCGGAAGATAAAcccggcccgg                                   <  1:3243681/93‑1 (MQ=255)

                                                        aaCTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGt                                 >  2:411078/1‑93 (MQ=255)

                                                        aaCTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGt                                 >  1:6655798/1‑93 (MQ=255)

                                                        aaCTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGt                                 >  1:5640738/1‑93 (MQ=255)

                                                         aCTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTa                                <  1:7659649/93‑1 (MQ=255)

                                                         aCTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTa                                <  2:1788849/93‑1 (MQ=255)

                                                         aCTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTa                                <  2:4869125/93‑1 (MQ=255)

                                                          cTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTAt                               <  1:5362659/93‑1 (MQ=255)

                                                          cTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTAt                               >  1:5830914/1‑93 (MQ=255)

                                                            ggCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGc                             >  1:2098825/1‑93 (MQ=255)

                                                               gcTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCAc                          <  2:3519792/93‑1 (MQ=255)

                                                                cTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCAcc                         >  2:2634857/1‑93 (MQ=255)

                                                                 ttCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCg                        >  1:4965163/1‑93 (MQ=255)

                                                                 ttCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCg                        >  1:201522/1‑93 (MQ=255)

                                                                    cTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCAc                     >  2:3794596/1‑93 (MQ=255)

                                                                     tGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACGCGGCCCGGGTATGCCACCGCACg                    >  2:4365074/1‑93 (MQ=255)

                                                                     tGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACg                    >  2:5871905/1‑93 (MQ=255)

                                                                      gcgcAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACgg                   <  1:4020660/93‑1 (MQ=255)

                                                                       cgcAACAATGATCGCTTTTCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGc                  <  1:4672026/93‑1 (MQ=255)

                                                                       cgcAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGc                  <  2:6877428/93‑1 (MQ=255)

                                                                       cgcAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGc                  >  2:6291638/1‑93 (MQ=255)

                                                                        gcAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCt                 <  1:7616520/93‑1 (MQ=255)

                                                                        gcAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCt                 >  2:743588/1‑93 (MQ=255)

                                                                        gcAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCt                 >  2:5335797/1‑93 (MQ=255)

                                                                         caacaaTGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTg                >  1:5110205/1‑93 (MQ=255)

                                                                          aacaaTGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTgg               <  1:1016443/93‑1 (MQ=255)

                                                                          aacaaTGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTgg               >  2:111323/1‑93 (MQ=255)

                                                                           acaaTGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGc              >  2:3008618/1‑93 (MQ=255)

                                                                           acaaTGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGc              <  2:701708/93‑1 (MQ=255)

                                                                              aTGATCTTTTTGCTTTTAAAACCCTGCGTTAATTGAAACACAATATTTGGAAAGAAAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTa           <  2:1422250/93‑1 (MQ=255)

                                                                                gATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTAcc         >  1:3383671/1‑93 (MQ=255)

                                                                                  tCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCa       >  1:3464537/1‑93 (MQ=255)

                                                                                    gCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGt     <  2:1704270/93‑1 (MQ=255)

                                                                                     cTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAggt    >  2:4827452/1‑91 (MQ=255)

                                                                                      tttGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCTCCGCACGGCTGGCGTACCCAGTTc   <  2:1958680/93‑1 (MQ=255)

                                                                                       ttGCCTTTCATATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCt  <  1:3835474/93‑1 (MQ=255)

                                                                                       ttGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCt  >  1:6866583/1‑93 (MQ=255)

AACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCT  >  NC_000913/2161040‑2161219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCTGGGT  >  NC_000913/2161038‑2161223

TGAACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCC                                                                                              >  SRR4302543.5782059/1‑94 (MQ=60)

 GAACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCT                                                                                             <  SRR4302543.7703238/94‑1 (MQ=60)

 GAACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCT                                                                                             >  SRR4302543.2002297/1‑94 (MQ=60)

  AACAGCGATTACAACTGTTAAGTTATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTT                                                                                            >  SRR4302543.1401113/1‑94 (MQ=60)

  AACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTT                                                                                            <  SRR4302543.4317479/94‑1 (MQ=60)

   ACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTT                                                                                           <  SRR4302543.4451159/94‑1 (MQ=60)

    CAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTC                                                                                          <  SRR4302543.1681059/94‑1 (MQ=60)

     AGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCA                                                                                         >  SRR4302543.4912772/1‑94 (MQ=60)

      GCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAG                                                                                        >  SRR4302543.1508029/1‑94 (MQ=60)

       CGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGA                                                                                       >  SRR4302543.4151117/1‑94 (MQ=60)

        GATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGAT                                                                                      >  SRR4302543.5900223/1‑94 (MQ=60)

        GATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGAT                                                                                      >  SRR4302543.1097210/1‑94 (MQ=60)

        GATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGAT                                                                                      >  SRR4302543.4335691/1‑94 (MQ=60)

          TTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCC                                                                                    >  SRR4302543.4508703/1‑94 (MQ=60)

          TTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCC                                                                                    <  SRR4302543.1156214/94‑1 (MQ=60)

          TTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCC                                                                                    <  SRR4302543.1156007/94‑1 (MQ=60)

           TACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCT                                                                                   <  SRR4302543.178487/94‑1 (MQ=60)

            ACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTG                                                                                  <  SRR4302543.3035523/94‑1 (MQ=60)

            ACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTG                                                                                  <  SRR4302543.7019518/94‑1 (MQ=60)

            ACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTG                                                                                  >  SRR4302543.3676205/1‑94 (MQ=60)

                CTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTT                                                                              <  SRR4302543.89361/94‑1 (MQ=60)

                CTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTT                                                                              >  SRR4302543.5656855/1‑94 (MQ=60)

                 TGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTC                                                                             <  SRR4302543.7085992/94‑1 (MQ=60)

                 TGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTC                                                                             >  SRR4302543.5562510/1‑94 (MQ=60)

                 TGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTC                                                                             >  SRR4302543.7632651/1‑94 (MQ=60)

                 TGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTC                                                                             >  SRR4302543.1189099/1‑94 (MQ=60)

                   TTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATGGCTTTGCCTTTCAGATCCTGCGTTCAT                                                                           >  SRR4302543.3638410/1‑94 (MQ=60)

                   TTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCAT                                                                           <  SRR4302543.3055046/94‑1 (MQ=60)

                   TTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCAT                                                                           <  SRR4302543.6191947/94‑1 (MQ=60)

                      AGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTG                                                                        >  SRR4302543.6502183/1‑94 (MQ=60)

                        TGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAA                                                                      >  SRR4302543.3763935/1‑94 (MQ=60)

                        TGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAA                                                                      >  SRR4302543.2915020/1‑94 (MQ=60)

                         GATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAAC                                                                     >  SRR4302543.4178148/1‑94 (MQ=60)

                          ATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACA                                                                    <  SRR4302543.1420222/94‑1 (MQ=60)

                           TGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACAC                                                                   <  SRR4302543.5771188/94‑1 (MQ=60)

                            GCTCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACG                                                                  <  SRR4302543.5941355/94‑1 (MQ=60)

                            GCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACG                                                                  <  SRR4302543.5350690/94‑1 (MQ=60)

                             CGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGA                                                                 >  SRR4302543.6326837/1‑94 (MQ=60)

                             CGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGA                                                                 >  SRR4302543.1334106/1‑94 (MQ=60)

                              GCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGAT                                                                <  SRR4302543.2486933/94‑1 (MQ=60)

                               CTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATA                                                               <  SRR4302543.7901587/94‑1 (MQ=60)

                               CTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATA                                                               >  SRR4302543.430135/1‑94 (MQ=60)

                               CTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATA                                                               >  SRR4302543.83/1‑94 (MQ=60)

                                TTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAA                                                              >  SRR4302543.867829/1‑94 (MQ=60)

                                TTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAA                                                              <  SRR4302543.7136208/94‑1 (MQ=60)

                                 TGGCGAGCAGTATGCACAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAAT                                                             <  SRR4302543.876744/94‑1 (MQ=60)

                                   GCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTC                                                           <  SRR4302543.1597604/94‑1 (MQ=60)

                                    CGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCG                                                          <  SRR4302543.6025889/94‑1 (MQ=60)

                                       GCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAA                                                       <  SRR4302543.4612364/94‑1 (MQ=60)

                                        CAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAG                                                      <  SRR4302543.4346589/94‑1 (MQ=60)

                                        CAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAG                                                      >  SRR4302543.7532824/1‑94 (MQ=60)

                                         AGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGA                                                     >  SRR4302543.2882564/1‑94 (MQ=60)

                                         AGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGA                                                     <  SRR4302543.3144966/94‑1 (MQ=60)

                                          GTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGAT                                                    <  SRR4302543.2239405/94‑1 (MQ=60)

                                          GTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGAT                                                    >  SRR4302543.3386912/1‑94 (MQ=60)

                                           TATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATA                                                   >  SRR4302543.7298908/1‑94 (MQ=60)

                                            ATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAA                                                  <  SRR4302543.7663195/94‑1 (MQ=60)

                                             TGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGCACACGATAATTCGGAAGATAAA                                                 <  SRR4302543.1344495/94‑1 (MQ=60)

                                             TGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAA                                                 <  SRR4302543.6427216/94‑1 (MQ=60)

                                              GCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAC                                                <  SRR4302543.7981626/94‑1 (MQ=60)

                                              GCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAAC                                                <  SRR4302543.2291283/94‑1 (MQ=60)

                                               CGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACC                                               <  SRR4302543.98004/94‑1 (MQ=60)

                                               CGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACC                                               <  SRR4302543.97999/94‑1 (MQ=60)

                                               CGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACC                                               >  SRR4302543.1671775/1‑94 (MQ=60)

                                                GCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCC                                              >  SRR4302543.4235343/1‑94 (MQ=60)

                                                GCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCC                                              >  SRR4302543.6177374/1‑94 (MQ=60)

                                                 CAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCG                                             <  SRR4302543.1467813/94‑1 (MQ=60)

                                                 CAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCG                                             >  SRR4302543.5629345/1‑94 (MQ=60)

                                                 CAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCG                                             <  SRR4302543.7138659/94‑1 (MQ=60)

                                                 CAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCG                                             <  SRR4302543.3419064/94‑1 (MQ=60)

                                                  AGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGG                                            <  SRR4302543.745946/94‑1 (MQ=60)

                                                   GCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGC                                           <  SRR4302543.5545995/94‑1 (MQ=60)

                                                    CATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCC                                          >  SRR4302543.4310476/1‑94 (MQ=60)

                                                    CATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCC                                          <  SRR4302543.469533/94‑1 (MQ=60)

                                                     ATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCC                                         <  SRR4302543.316598/94‑1 (MQ=60)

                                                       GGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGG                                       >  SRR4302543.95106/1‑94 (MQ=60)

                                                        GCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCTTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGG                                      <  SRR4302543.3273015/94‑1 (MQ=60)

                                                         CAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGT                                     >  SRR4302543.5692160/1‑94 (MQ=60)

                                                         CAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGT                                     >  SRR4302543.4861925/1‑94 (MQ=60)

                                                         CAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGT                                     >  SRR4302543.6718640/1‑94 (MQ=60)

                                                          AACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTA                                    <  SRR4302543.3736320/94‑1 (MQ=60)

                                                           ACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTAT                                   <  SRR4302543.7732250/94‑1 (MQ=60)

                                                           ACTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTAT                                   >  SRR4302543.5883950/1‑94 (MQ=60)

                                                            CTGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATG                                  <  SRR4302543.5411194/94‑1 (MQ=60)

                                                             TGGCGCTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGC                                 >  SRR4302543.2117594/1‑94 (MQ=60)

                                                                  CTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCG                            >  SRR4302543.5010360/1‑94 (MQ=60)

                                                                  CTTCCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCG                            >  SRR4302543.203222/1‑94 (MQ=60)

                                                                     GCTGCGAAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTAGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCTCCGCAC                         <  SRR4302543.3095422/94‑1 (MQ=60)

                                                                     CCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCAC                         <  SRR4302543.3191620/94‑1 (MQ=60)

                                                                     CCTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCAC                         <  SRR4302543.6754209/94‑1 (MQ=60)

                                                                      CTGCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACG                        >  SRR4302543.4547056/1‑94 (MQ=60)

                                                                        GCGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGC                      <  SRR4302543.4056572/94‑1 (MQ=60)

                                                                         CGCAACAATGATCGCTTTTCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCT                     <  SRR4302543.4714614/94‑1 (MQ=60)

                                                                         CGCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCT                     <  SRR4302543.569151/94‑1 (MQ=60)

                                                                          GCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTG                    <  SRR4302543.7688818/94‑1 (MQ=60)

                                                                          GCAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTG                    >  SRR4302543.5156446/1‑94 (MQ=60)

                                                                           CAACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGG                   <  SRR4302543.3717425/94‑1 (MQ=60)

                                                                            AACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGC                  <  SRR4302543.1025433/94‑1 (MQ=60)

                                                                             ACAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCG                 <  SRR4302543.3978511/94‑1 (MQ=60)

                                                                              CAATGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGT                <  SRR4302543.7699338/94‑1 (MQ=60)

                                                                                 TGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCTGGCCCGGGTATGCCACCGCACGGCTGGCGTACC             >  SRR4302543.7675710/1‑94 (MQ=60)

                                                                                 TGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACC             >  SRR4302543.3414016/1‑94 (MQ=60)

                                                                                 TGATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACC             >  SRR4302543.556355/1‑94 (MQ=60)

                                                                                  GATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCC            >  SRR4302543.6657629/1‑94 (MQ=60)

                                                                                  GATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCC            >  SRR4302543.7826713/1‑94 (MQ=60)

                                                                                   ATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCA           >  SRR4302543.3495570/1‑94 (MQ=60)

                                                                                   ATCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCA           >  SRR4302543.3806481/1‑94 (MQ=60)

                                                                                    TCGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAG          <  SRR4302543.2063838/94‑1 (MQ=60)

                                                                                     CGCTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGT         <  SRR4302543.7407171/94‑1 (MQ=60)

                                                                                       CTTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTC       <  SRR4302543.5115728/94‑1 (MQ=60)

                                                                                        TTTGCCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCT      >  SRR4302543.6931271/1‑94 (MQ=60)

                                                                                         TTGCCTTTCATATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCTG     <  SRR4302543.3869319/94‑1 (MQ=60)

                                                                                            CCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCTGGGT  <  SRR4302543.1587613/94‑1 (MQ=60)

                                                                                            CCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCTGGGT  >  SRR4302543.4042908/1‑94 (MQ=60)

                                                                                            CCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCTGGGT  <  SRR4302543.7005144/94‑1 (MQ=60)

TGAACAGCGATTACAACTGTTAAGTGATGCGCTTGGCGAGCAGTATGCGCAGCATGGCAACTGGCGCTTCCTGCGCAACAATGATCGCTTTGTCTTTCAGATCCTGCGTTCATTTGAACACGATAATTCGGAAGATAAACCCGGCCCGGGTATGCCACCGCACGGCTGGCGTACCCAGTTCTGGGT  >  NC_000913/2161038‑2161223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`