Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,756 T→C T196A (ACT→GCT)  gatZ ← putative tagatose‑6‑phosphate kinase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,7560TC100.0% 722.5 / NA 225T196A (ACT→GCT) gatZputative tagatose‑6‑phosphate kinase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (114/111);  total (114/111)

Only 100 of 229 total aligned reads displayed.

CCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACAT  >  NC_000913/2173666‑2173845
                                                                                          |                                                                                         
ccACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCAt                                                                                         <  1:4456459/93‑1 (MQ=255)
 cacaCCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCAtt                                                                                        <  1:5326929/93‑1 (MQ=255)
  acacCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTg                                                                                       >  2:5116466/1‑93 (MQ=255)
  acacCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTg                                                                                       >  1:7220970/1‑93 (MQ=255)
  acacCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTg                                                                                       >  1:2489741/1‑93 (MQ=255)
   cacCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTgg                                                                                      <  2:6413304/93‑1 (MQ=255)
   cacCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTgg                                                                                      <  2:6266667/93‑1 (MQ=255)
    ccccGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTggc                                                                                     <  2:3035446/92‑1 (MQ=255)
    ccccGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTggc                                                                                     <  2:1215335/92‑1 (MQ=255)
    acCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTggc                                                                                     >  2:1496648/1‑93 (MQ=255)
     cccGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTggcg                                                                                    <  2:910101/93‑1 (MQ=255)
     cccGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTggcg                                                                                    >  1:6072457/1‑93 (MQ=255)
      ccGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTCTGATGCGTACGTAAAGCATTggcgc                                                                                   >  2:5946822/1‑92 (MQ=255)
       cGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTggcggc                                                                                  >  1:1056300/1‑93 (MQ=255)
        ggCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCa                                                                                 >  1:5051124/1‑93 (MQ=255)
         gCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCAt                                                                                >  1:3665216/1‑93 (MQ=255)
          cTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATc                                                                               >  1:1017973/1‑93 (MQ=255)
            gCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCtt                                                                             <  2:3906476/93‑1 (MQ=255)
             caccacGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTc                                                                            >  2:2665638/1‑93 (MQ=255)
             caccacGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTc                                                                            <  1:407646/93‑1 (MQ=255)
              accacGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCa                                                                           <  1:3393162/93‑1 (MQ=255)
              accacGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCa                                                                           >  1:2019008/1‑93 (MQ=255)
               ccacGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCaa                                                                          <  1:6655196/93‑1 (MQ=255)
                cacGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAAc                                                                         <  1:6354859/93‑1 (MQ=255)
                 acGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACa                                                                        <  1:5189948/93‑1 (MQ=255)
                    aTGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATgg                                                                     <  2:6213336/93‑1 (MQ=255)
                    aTGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATgg                                                                     <  2:5018984/93‑1 (MQ=255)
                           tCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGAtgt                                                              <  2:7711267/93‑1 (MQ=255)
                            cacaCGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGAtgtg                                                             <  1:3937055/93‑1 (MQ=255)
                             acacGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATgtgt                                                            >  2:4116771/1‑93 (MQ=255)
                              cacGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTa                                                           >  1:4595343/1‑93 (MQ=255)
                              cacGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCCTCAACATGGGTGATGTGTa                                                           >  2:7048195/1‑93 (MQ=255)
                                cGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTact                                                         <  2:185688/93‑1 (MQ=255)
                                cGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTact                                                         <  1:7547027/93‑1 (MQ=255)
                                 gtgtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactg                                                        >  1:4380716/1‑93 (MQ=255)
                                 gtgtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactg                                                        >  2:7599197/1‑93 (MQ=255)
                                  tgtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactga                                                       <  1:886594/93‑1 (MQ=255)
                                   gtTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactgac                                                      <  2:525572/93‑1 (MQ=255)
                                    ttAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactgact                                                     >  2:6638379/1‑93 (MQ=255)
                                    ttAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactgact                                                     >  1:6775176/1‑93 (MQ=255)
                                     tAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactgactg                                                    <  2:7147494/93‑1 (MQ=255)
                                     tAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTactgactg                                                    >  2:604396/1‑93 (MQ=255)
                                       aCGCCTCTGTCAGCCCACGGGCAATAAAGTCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGaa                                                  <  1:4482743/93‑1 (MQ=255)
                                       aCGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGaa                                                  <  1:1559239/93‑1 (MQ=255)
                                       aCGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGaa                                                  <  1:3138810/93‑1 (MQ=255)
                                       aCGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGaa                                                  >  1:2923880/1‑93 (MQ=255)
                                        cGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAt                                                 >  2:6523041/1‑93 (MQ=255)
                                        cGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAt                                                 <  2:5843943/93‑1 (MQ=255)
                                         gCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGCCTAAATg                                                >  1:1245221/1‑93 (MQ=255)
                                         gCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATg                                                <  1:7771090/93‑1 (MQ=255)
                                         gCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATg                                                <  1:2381799/93‑1 (MQ=255)
                                          ccTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATgg                                               <  1:3016319/93‑1 (MQ=255)
                                          ccTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATgg                                               <  2:4096861/93‑1 (MQ=255)
                                          ccTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGGGTACTGACTGTATgg                                               >  2:2185247/1‑93 (MQ=255)
                                           ctctGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGGGATGTGTACTGACTGAATAgc                                              >  1:130838/1‑93 (MQ=255)
                                            tctGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGGACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGgcg                                             >  2:3234564/1‑93 (MQ=255)
                                              tGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCt                                           >  2:4599019/1‑93 (MQ=255)
                                               gTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTTGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTg                                          >  2:4915872/1‑93 (MQ=255)
                                               gTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTg                                          <  1:4788630/93‑1 (MQ=255)
                                               gTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTg                                          >  2:2121709/1‑93 (MQ=255)
                                                 cAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGc                                        <  2:2126702/93‑1 (MQ=255)
                                                 cAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGc                                        >  1:7334268/1‑93 (MQ=255)
                                                  aGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGcc                                       <  1:5578696/93‑1 (MQ=255)
                                                   gCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCt                                      >  2:6946433/1‑93 (MQ=255)
                                                      cACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCAc                                   <  1:191842/93‑1 (MQ=255)
                                                      cACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCAc                                   >  2:6189088/1‑93 (MQ=255)
                                                        cGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCg                                 >  2:7390577/1‑93 (MQ=255)
                                                         gggCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGc                                >  2:7769830/1‑93 (MQ=255)
                                                            cAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccg                             <  2:4713176/93‑1 (MQ=255)
                                                             aaTAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccgg                            <  2:1436260/93‑1 (MQ=255)
                                                              aTAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccgga                           >  2:294690/1‑93 (MQ=255)
                                                              aTAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccgga                           >  1:1687873/1‑93 (MQ=255)
                                                               tAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccggaa                          >  2:1586047/1‑93 (MQ=255)
                                                                aaaGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccggaac                         <  1:3870773/93‑1 (MQ=255)
                                                                 aaGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccggaacc                        >  2:3802084/1‑93 (MQ=255)
                                                                  aGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccggaaccg                       <  1:7416489/93‑1 (MQ=255)
                                                                   ggCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccggaaccgg                      >  2:1021606/1‑93 (MQ=255)
                                                                     ccTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCccggaaccggaa                    <  1:5387126/93‑1 (MQ=255)
                                                                      cTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCcggaaccggaac                   >  2:2557769/1‑93 (MQ=255)
                                                                       ttttGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACt                  >  1:6965206/1‑93 (MQ=255)
                                                                       ttttGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACt                  >  1:17447/1‑93 (MQ=255)
                                                                         ttGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTc                >  2:4461533/1‑93 (MQ=255)
                                                                          tGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCg               <  1:6183291/93‑1 (MQ=255)
                                                                           gATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCgg              <  1:4199734/93‑1 (MQ=255)
                                                                           gATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCgg              <  1:2324761/93‑1 (MQ=255)
                                                                           gATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCgg              >  2:2366504/1‑93 (MQ=255)
                                                                            aTGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGt             <  1:4006771/93‑1 (MQ=255)
                                                                               cgtacgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGcc          >  2:1951242/1‑93 (MQ=255)
                                                                               cgtacgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGcc          <  2:2914719/93‑1 (MQ=255)
                                                                               cgtacgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGcc          <  1:7164885/93‑1 (MQ=255)
                                                                                gtacgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCa         >  2:5715626/1‑93 (MQ=255)
                                                                                gtacgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCa         <  1:454770/93‑1 (MQ=255)
                                                                                 tacgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCaa        >  2:7190351/1‑93 (MQ=255)
                                                                                 tacgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCaa        >  1:2840950/1‑93 (MQ=255)
                                                                                  acgtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAAt       >  2:3577402/1‑93 (MQ=255)
                                                                                    gtaAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGa     >  2:1715688/1‑93 (MQ=255)
                                                                                     taAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGAc    >  2:5821712/1‑93 (MQ=255)
                                                                                      aaaGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACa   >  2:3514016/1‑93 (MQ=255)
                                                                                      aaaGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACa   >  1:2693307/1‑93 (MQ=255)
                                                                                      aaaGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACa   <  1:2727591/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACAT  >  NC_000913/2173666‑2173845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCT  >  NC_000913/2173663‑2173849
                                                                                             |                                                                                             
ATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGC                                                                                               >  SRR4302543.2286666/1‑94 (MQ=60)
ATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGC                                                                                               >  SRR4302543.1326560/1‑94 (MQ=60)
 TTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCA                                                                                              <  SRR4302543.4743840/94‑1 (MQ=60)
 TTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCA                                                                                              <  SRR4302543.4362598/94‑1 (MQ=60)
  TCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCAT                                                                                             <  SRR4302543.3578936/94‑1 (MQ=60)
   CCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATT                                                                                            <  SRR4302543.3832758/94‑1 (MQ=60)
   CCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATT                                                                                            <  SRR4302543.4496834/94‑1 (MQ=60)
    CACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTG                                                                                           >  SRR4302543.7290956/1‑94 (MQ=60)
    CACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTG                                                                                           >  SRR4302543.2512301/1‑94 (MQ=60)
    CACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTG                                                                                           <  SRR4302543.5375095/94‑1 (MQ=60)
      CACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGC                                                                                         <  SRR4302543.2725324/94‑1 (MQ=60)
      CACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGC                                                                                         <  SRR4302543.3286487/94‑1 (MQ=60)
      CACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGCAAAGCATTGGC                                                                                         >  SRR4302543.567090/1‑94 (MQ=60)
       ACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCG                                                                                        >  SRR4302543.6130008/1‑94 (MQ=60)
       ACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCG                                                                                        >  SRR4302543.1777252/1‑94 (MQ=60)
        CCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGG                                                                                       >  SRR4302543.1593246/1‑94 (MQ=60)
         CCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGC                                                                                      >  SRR4302543.1065780/1‑94 (MQ=60)
          CGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCA                                                                                     >  SRR4302543.5097051/1‑94 (MQ=60)
           GGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCAT                                                                                    >  SRR4302543.3697399/1‑94 (MQ=60)
            GCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATC                                                                                   <  SRR4302543.7435258/94‑1 (MQ=60)
            GCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATC                                                                                   >  SRR4302543.1026976/1‑94 (MQ=60)
              TGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTT                                                                                 >  SRR4302543.2360630/1‑94 (MQ=60)
              TGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTT                                                                                 >  SRR4302543.6707808/1‑94 (MQ=60)
              TGCACCACGATGGCAATAACACGGGTTAAAGCCTCTGTCAGCCCACGGGCAATAAAGTCCTTTTGATGCGTACGTAAAGCATTGGCGGAATCTT                                                                                 >  SRR4302543.4388262/1‑94 (MQ=60)
                CACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCA                                                                               <  SRR4302543.411406/94‑1 (MQ=60)
                CACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCA                                                                               >  SRR4302543.2037149/1‑94 (MQ=60)
                 ACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAA                                                                              <  SRR4302543.3423584/94‑1 (MQ=60)
                  CCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAAC                                                                             >  SRR4302543.2960131/1‑94 (MQ=60)
                  CCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAAC                                                                             <  SRR4302543.6718026/94‑1 (MQ=60)
                   CACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACA                                                                            <  SRR4302543.6414611/94‑1 (MQ=60)
                    ACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACAT                                                                           <  SRR4302543.5236754/94‑1 (MQ=60)
                    ACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACAT                                                                           <  SRR4302543.5283309/94‑1 (MQ=60)
                    ACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACAT                                                                           <  SRR4302543.4298420/94‑1 (MQ=60)
                     CGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATG                                                                          <  SRR4302543.4007366/94‑1 (MQ=60)
                      GATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGG                                                                         <  SRR4302543.2134537/94‑1 (MQ=60)
                      GATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGG                                                                         >  SRR4302543.2787417/1‑94 (MQ=60)
                       ATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGG                                                                        >  SRR4302543.72442/1‑94 (MQ=60)
                         agagATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTG                                                                      <  SRR4302543.4621877/90‑1 (MQ=60)
                           CAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGAT                                                                    >  SRR4302543.4566706/1‑94 (MQ=60)
                           CAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGAT                                                                    <  SRR4302543.1325107/94‑1 (MQ=60)
                             ATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGT                                                                  >  SRR4302543.5225419/1‑94 (MQ=60)
                             ATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGT                                                                  <  SRR4302543.5377381/94‑1 (MQ=60)
                               CACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGT                                                                <  SRR4302543.3971846/94‑1 (MQ=60)
                                ACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTA                                                               >  SRR4302543.4637077/1‑94 (MQ=60)
                                 CACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACTTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTAC                                                              >  SRR4302543.7945770/1‑94 (MQ=60)
                                  ACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACT                                                             <  SRR4302543.1879026/94‑1 (MQ=60)
                                   CGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTG                                                            >  SRR4302543.4420423/1‑94 (MQ=60)
                                   CGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTG                                                            <  SRR4302543.7618925/94‑1 (MQ=60)
                                   CGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTG                                                            <  SRR4302543.7618924/94‑1 (MQ=60)
                                    GTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGA                                                           >  SRR4302543.7472351/1‑94 (MQ=60)
                                     TGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGAC                                                          <  SRR4302543.894447/94‑1 (MQ=60)
                                      GTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACT                                                         <  SRR4302543.5551376/94‑1 (MQ=60)
                                      GTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACT                                                         <  SRR4302543.2003268/94‑1 (MQ=60)
                                      GTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACT                                                         >  SRR4302543.6839178/1‑94 (MQ=60)
                                         AACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAA                                                      >  SRR4302543.6786984/1‑94 (MQ=60)
                                         AACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAA                                                      >  SRR4302543.2950438/1‑94 (MQ=60)
                                          ACGCCTCTGTCAGCCCACGGGCAATAAAGTCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAT                                                     <  SRR4302543.4523457/94‑1 (MQ=60)
                                          ACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAT                                                     <  SRR4302543.4523458/94‑1 (MQ=60)
                                          ACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAT                                                     <  SRR4302543.1573169/94‑1 (MQ=60)
                                          ACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAAT                                                     <  SRR4302543.3167041/94‑1 (MQ=60)
                                           CGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGCCTAAATG                                                    >  SRR4302543.1256398/1‑94 (MQ=60)
                                            GCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGG                                                   <  SRR4302543.2403365/94‑1 (MQ=60)
                                            GCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGG                                                   <  SRR4302543.451136/94‑1 (MQ=60)
                                            GCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGG                                                   <  SRR4302543.7844398/94‑1 (MQ=60)
                                             CCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGTGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGC                                                  <  SRR4302543.5231250/94‑1 (MQ=60)
                                             CCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGC                                                  <  SRR4302543.3043604/94‑1 (MQ=60)
                                             CCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGC                                                  >  SRR4302543.905929/1‑94 (MQ=60)
                                             CCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGGGATGTGTACTGACTGAATAGC                                                  >  SRR4302543.132008/1‑94 (MQ=60)
                                                CTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCT                                               <  SRR4302543.3943290/94‑1 (MQ=60)
                                                CTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCT                                               >  SRR4302543.244762/1‑94 (MQ=60)
                                                CTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCT                                               <  SRR4302543.3943289/94‑1 (MQ=60)
                                                  GTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGG                                             <  SRR4302543.4832398/94‑1 (MQ=60)
                                                  GTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGT‑AAGCATTGGCGGCATCTTCAACATtggggatgaagatgccgccaatactttacgt                                            >  SRR4302543.4434548/1‑63 (MQ=60)
                                                   TCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGC                                            >  SRR4302543.7404838/1‑94 (MQ=60)
                                                     AGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCT                                          <  SRR4302543.5629482/94‑1 (MQ=60)
                                                     AGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCT                                          <  SRR4302543.5629483/94‑1 (MQ=60)
                                                        CCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCAC                                       >  SRR4302543.1525325/1‑94 (MQ=60)
                                                         CACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACC                                      <  SRR4302543.7317241/94‑1 (MQ=60)
                                                         CACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACC                                      <  SRR4302543.193460/94‑1 (MQ=60)
                                                             GGCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACggccg                                  >  SRR4302543.2686995/1‑89 (MQ=60)
                                                              GCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCG                                 <  SRR4302543.6327209/94‑1 (MQ=60)
                                                              GCAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCG                                 <  SRR4302543.1046723/94‑1 (MQ=60)
                                                               CAATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGG                                >  SRR4302543.3443361/1‑94 (MQ=60)
                                                                AATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGA                               >  SRR4302543.1703030/1‑94 (MQ=60)
                                                                AATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGA                               >  SRR4302543.1237695/1‑94 (MQ=60)
                                                                 ATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAC                              >  SRR4302543.6214445/1‑94 (MQ=60)
                                                                 ATAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAA                              >  SRR4302543.4649879/1‑94 (MQ=60)
                                                                  TAAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAAC                             <  SRR4302543.4065113/94‑1 (MQ=60)
                                                                   AAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACC                            <  SRR4302543.3904953/94‑1 (MQ=60)
                                                                   AAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACC                            <  SRR4302543.6924785/94‑1 (MQ=60)
                                                                   AAAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACC                            <  SRR4302543.7227346/94‑1 (MQ=60)
                                                                    AAGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCG                           <  SRR4302543.7147172/94‑1 (MQ=60)
                                                                     AGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGG                          >  SRR4302543.1270777/1‑94 (MQ=60)
                                                                     AGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGG                          >  SRR4302543.3358838/1‑94 (MQ=60)
                                                                     AGGCCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGG                          <  SRR4302543.7487636/94‑1 (MQ=60)
                                                                        CCTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAAC                       <  SRR4302543.5436064/94‑1 (MQ=60)
                                                                         CTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACT                      >  SRR4302543.315903/1‑94 (MQ=60)
                                                                         CTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACT                      >  SRR4302543.17609/1‑94 (MQ=60)
                                                                         CTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACT                      <  SRR4302543.2453773/94‑1 (MQ=60)
                                                                         CTTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACT                      >  SRR4302543.7030752/1‑94 (MQ=60)
                                                                         CTTTTGATGCGTACGTAAAGAATTGGCGGCATCTTCAACCTGGGTGATGTGTACTGACTGCATGGGGCTGGgttcagcgacggggacgggcgct                      >  SRR4302543.7030753/1‑71 (MQ=60)
                                                                          TTTTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTT                     >  SRR4302543.4207628/1‑94 (MQ=60)
                                                                            TTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCG                   <  SRR4302543.1740596/94‑1 (MQ=60)
                                                                            TTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCG                   <  SRR4302543.2872225/94‑1 (MQ=60)
                                                                            TTGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCG                   >  SRR4302543.2833367/1‑94 (MQ=60)
                                                                             TGATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGG                  <  SRR4302543.6241723/94‑1 (MQ=60)
                                                                              GATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGT                 <  SRR4302543.2345889/94‑1 (MQ=60)
                                                                              GATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGT                 <  SRR4302543.4237812/94‑1 (MQ=60)
                                                                               ATGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTG                <  SRR4302543.4042532/94‑1 (MQ=60)
                                                                                TGCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGC               <  SRR4302543.6269642/94‑1 (MQ=60)
                                                                                 GCGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCC              >  SRR4302543.4450909/1‑94 (MQ=60)
                                                                                  CGTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCA             <  SRR4302543.7234482/94‑1 (MQ=60)
                                                                                   GTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAA            <  SRR4302543.459003/94‑1 (MQ=60)
                                                                                   GTACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAA            >  SRR4302543.2866916/1‑94 (MQ=60)
                                                                                    TACGTAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAAT           >  SRR4302543.7622808/1‑94 (MQ=60)
                                                                                        TAAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACA       >  SRR4302543.2717724/1‑94 (MQ=60)
                                                                                         AAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACAT      <  SRR4302543.2752409/94‑1 (MQ=60)
                                                                                         AAAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACAT      <  SRR4302543.4905285/94‑1 (MQ=60)
                                                                                          AAGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATA     <  SRR4302543.927513/94‑1 (MQ=60)
                                                                                           AGCATTGGCGGCATCTTCAACATGGGTTATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAG    <  SRR4302543.964864/94‑1 (MQ=60)
                                                                                           AGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAG    >  SRR4302543.3693690/1‑94 (MQ=60)
                                                                                           AGCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAG    <  SRR4302543.3197761/94‑1 (MQ=60)
                                                                                            GCATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGC   <  SRR4302543.6480606/94‑1 (MQ=60)
                                                                                             CATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCT  >  SRR4302543.2824290/1‑94 (MQ=60)
                                                                                             CATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCT  >  SRR4302543.1019771/1‑94 (MQ=60)
                                                                                             CATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCT  >  SRR4302543.1019770/1‑94 (MQ=60)
                                                                                             CATTGGCGGCATCTGCAACATGGGGGATGTGTACTGACTGAATGGCGCCGGCCTCACCGCCCGGAACCGGAACACCGGCGCCAATGACATAGCC  >  SRR4302543.6090990/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTCCACACCCGGCTGCACCACGATGGCAATCACACGTGTTAACGCCTCTGTCAGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCT  >  NC_000913/2173663‑2173849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑