Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,175,131 G→A Q34* (CAA→TAA)  gatY ← tagatose‑bisphosphate aldolase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,175,1310GA100.0% 684.0 / NA 190Q34* (CAA→TAA) gatYtagatose‑bisphosphate aldolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (85/105);  total (85/105)

Only 100 of 198 total aligned reads displayed.

GATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGT  >  NC_000913/2175041‑2175220
                                                                                          |                                                                                         
gATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACa                                                                                         <  1:1532224/93‑1 (MQ=255)
gATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACa                                                                                         >  2:7855368/1‑93 (MQ=255)
 aTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACAt                                                                                        <  2:1242363/93‑1 (MQ=255)
   tttCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCg                                                                                      >  1:3183094/1‑93 (MQ=255)
   tttCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCg                                                                                      <  2:6115895/93‑1 (MQ=255)
     tCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGtt                                                                                    <  1:2925198/93‑1 (MQ=255)
     tCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGtt                                                                                    <  2:7520489/93‑1 (MQ=255)
      cTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGttt                                                                                   <  2:4533172/93‑1 (MQ=255)
       tGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTc                                                                                  <  1:3121730/93‑1 (MQ=255)
       tGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTc                                                                                  <  1:3909645/93‑1 (MQ=255)
         tACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCga                                                                                <  1:6973280/93‑1 (MQ=255)
         tACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCga                                                                                <  1:1880016/93‑1 (MQ=255)
           ccAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCgaga                                                                              <  2:331048/93‑1 (MQ=255)
            cAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGAt                                                                             >  2:6073323/1‑93 (MQ=255)
            cAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGAt                                                                             <  1:202182/93‑1 (MQ=255)
              gCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATtg                                                                           >  2:4824101/1‑93 (MQ=255)
                 tGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGa                                                                        <  1:1903949/93‑1 (MQ=255)
                  gAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGaa                                                                       >  1:5984272/1‑93 (MQ=255)
                  gAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGaa                                                                       <  1:289240/93‑1 (MQ=255)
                    gTAAATGTGCCAGGCTTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACAGCGTTTCGAGATTGTGAata                                                                     <  2:4025039/93‑1 (MQ=255)
                    gTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAata                                                                     >  1:1148615/1‑93 (MQ=255)
                    gTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAata                                                                     <  2:5127520/93‑1 (MQ=255)
                    gTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAata                                                                     <  2:2652583/93‑1 (MQ=255)
                    gTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAata                                                                     <  2:5238630/93‑1 (MQ=255)
                    gTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGCGAata                                                                     <  2:1461193/93‑1 (MQ=255)
                      aaaTGTGCCAGGCGTTCCGGCGATGATGACCGGCGCGTGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATAtt                                                                   <  1:5501420/93‑1 (MQ=255)
                      aaaTGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATAtt                                                                   <  2:4246969/93‑1 (MQ=255)
                       aaTGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTg                                                                  >  1:296393/1‑93 (MQ=255)
                        aTGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGa                                                                 >  1:4452808/1‑93 (MQ=255)
                         tgtgCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGaa                                                                <  2:2258893/93‑1 (MQ=255)
                         tgtgCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGaa                                                                <  2:1635295/93‑1 (MQ=255)
                         tgtgCCAGGCGTGCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGaa                                                                <  2:819984/93‑1 (MQ=255)
                          gtgCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAAt                                                               <  2:4043200/93‑1 (MQ=255)
                             ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATgac                                                            >  1:4858142/1‑91 (MQ=255)
                             ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGcc                                                            <  2:3123040/93‑1 (MQ=255)
                             ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGcc                                                            <  1:6919717/93‑1 (MQ=255)
                             ccAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGcc                                                            <  1:1232033/93‑1 (MQ=255)
                              cAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCg                                                           >  2:7304223/1‑93 (MQ=255)
                              cAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCg                                                           >  1:2115500/1‑93 (MQ=255)
                              cAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCg                                                           >  1:844844/1‑93 (MQ=255)
                                  cGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAAc                                                       <  1:1777585/93‑1 (MQ=255)
                                   gTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAAcc                                                      >  2:4185673/1‑93 (MQ=255)
                                   gTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAAcc                                                      <  1:5662440/93‑1 (MQ=255)
                                    ttCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCg                                                     >  1:654620/1‑93 (MQ=255)
                                    ttCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCg                                                     >  1:1027220/1‑93 (MQ=255)
                                      ccGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCa                                                   >  2:4726383/1‑93 (MQ=255)
                                         gCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATaa                                                >  1:3664096/1‑93 (MQ=255)
                                         gCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATaa                                                >  2:6234710/1‑93 (MQ=255)
                                          cGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAc                                               <  1:2532824/93‑1 (MQ=255)
                                            atgatgACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAccg                                             <  2:689471/93‑1 (MQ=255)
                                             tgatgaCCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAccgc                                            >  2:4988716/1‑93 (MQ=255)
                                             tgatgaCCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAccgc                                            <  1:5587224/93‑1 (MQ=255)
                                              gatgaCCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAccgcc                                           <  2:2513846/93‑1 (MQ=255)
                                              gactaCCGGGGCATGCAGGTTTGCAGCGCTTTGTGCCACCACTTATATCGTTTCGAGATTGAGAATATTGAAAGCCGGACCCGCATACccgcc                                           <  2:359174/89‑1 (MQ=255)
                                               atgaCCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAccgccg                                          >  1:4654201/1‑93 (MQ=255)
                                                tgaCCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACcgccgc                                         >  2:3224771/1‑93 (MQ=255)
                                                tgaCCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACcgccgc                                         <  1:5172268/93‑1 (MQ=255)
                                                         cATGCAGGTTGGCAGCGGTTTCTACCACCTCTTACATCGTATCGAGATTGTGAATATTGAATGCCGGATCCGCATAACCGCCGCGCTGTGCgt                                <  2:5506086/93‑1 (MQ=255)
                                                         cATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgt                                >  1:5109245/1‑93 (MQ=255)
                                                          aTGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgtt                               <  1:7592700/93‑1 (MQ=255)
                                                           tGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgttg                              >  2:1186818/1‑93 (MQ=255)
                                                           tGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgttg                              >  1:7430593/1‑93 (MQ=255)
                                                           tGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgttg                              >  1:2340706/1‑93 (MQ=255)
                                                           tGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgttg                              >  1:6162741/1‑93 (MQ=255)
                                                           tGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCCGAACCGCATAACCGCCGCGCTGTGCgttg                              >  2:40862/1‑93 (MQ=255)
                                                             cAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCgttgtt                            >  2:5922306/1‑93 (MQ=255)
                                                               ggTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCa                          <  1:4015234/93‑1 (MQ=255)
                                                               ggTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCa                          <  1:6697444/93‑1 (MQ=255)
                                                               ggTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCa                          >  1:6134993/1‑93 (MQ=255)
                                                               ggTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAACGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCa                          <  1:2166394/93‑1 (MQ=255)
                                                                  tGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGct                       >  1:6191972/1‑92 (MQ=255)
                                                                    gCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATc                     <  1:2467322/93‑1 (MQ=255)
                                                                    gCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATc                     <  2:7561853/93‑1 (MQ=255)
                                                                     cAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCt                    <  2:920079/93‑1 (MQ=255)
                                                                     cAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCt                    >  1:2941154/1‑93 (MQ=255)
                                                                       gCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGc                  >  1:3202072/1‑93 (MQ=255)
                                                                       gCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGc                  <  1:572772/93‑1 (MQ=255)
                                                                       gCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGc                  >  1:6483538/1‑93 (MQ=255)
                                                                         ggTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCtt                <  2:6940783/93‑1 (MQ=255)
                                                                          gTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCttt               <  2:7373829/93‑1 (MQ=255)
                                                                           tttCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTg              <  1:419332/93‑1 (MQ=255)
                                                                            ttCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGt             <  2:2673555/93‑1 (MQ=255)
                                                                            ttCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGt             <  1:7585856/93‑1 (MQ=255)
                                                                             tCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTc            <  2:4220519/93‑1 (MQ=255)
                                                                             tCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTc            <  2:3820261/93‑1 (MQ=255)
                                                                             tCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTc            <  2:2801362/93‑1 (MQ=255)
                                                                               tACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGa          >  1:6668591/1‑93 (MQ=255)
                                                                                 ccaccaCTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATa        <  1:3259891/93‑1 (MQ=255)
                                                                                 ccaccaCTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATa        <  2:7066874/93‑1 (MQ=255)
                                                                                  caccacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATAc       <  2:604969/93‑1 (MQ=255)
                                                                                   accacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATAcc      >  1:600590/1‑93 (MQ=255)
                                                                                   accacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATAcc      <  1:6848612/93‑1 (MQ=255)
                                                                                    ccacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCa     >  1:2764812/1‑93 (MQ=255)
                                                                                    ccacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCa     >  2:6436528/1‑93 (MQ=255)
                                                                                     cacTTGGATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCAc    <  2:5417814/93‑1 (MQ=255)
                                                                                     cacTTCCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCAc    <  2:4503112/93‑1 (MQ=255)
                                                                                     cacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCAc    >  1:3855994/1‑93 (MQ=255)
                                                                                     cacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCAc    >  2:5947718/1‑93 (MQ=255)
                                                                                     cacTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCAc    <  2:455433/93‑1 (MQ=255)
                                                                                       cTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGt  >  1:4540977/1‑93 (MQ=255)
                                                                                          |                                                                                         
GATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGT  >  NC_000913/2175041‑2175220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAT  >  NC_000913/2175039‑2175224
                                                                                            |                                                                                             
CAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTAC                                                                                              <  SRR4302543.1524246/94‑1 (MQ=60)
 AGATTTTCTGTACCAGCATGAGTCAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACA                                                                                             <  SRR4302543.1957262/94‑1 (MQ=60)
  GATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACAT                                                                                            <  SRR4302543.5155008/94‑1 (MQ=60)
  GATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACAT                                                                                            <  SRR4302543.1545959/94‑1 (MQ=60)
  GATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACAT                                                                                            <  SRR4302543.1885549/94‑1 (MQ=60)
   ATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATC                                                                                           >  SRR4302543.1898213/1‑94 (MQ=60)
    TTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCG                                                                                          >  SRR4302543.3211800/1‑94 (MQ=60)
    TTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCG                                                                                          <  SRR4302543.2387479/94‑1 (MQ=60)
    TTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCG                                                                                          <  SRR4302543.293805/94‑1 (MQ=60)
      TTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTT                                                                                        >  SRR4302543.2514643/1‑94 (MQ=60)
       TCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTT                                                                                       <  SRR4302543.2951770/94‑1 (MQ=60)
         TGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCG                                                                                     <  SRR4302543.4638133/94‑1 (MQ=60)
         TGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCG                                                                                     <  SRR4302543.4039278/94‑1 (MQ=60)
         TGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCG                                                                                     <  SRR4302543.3944161/94‑1 (MQ=60)
         TGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCG                                                                                     <  SRR4302543.3149806/94‑1 (MQ=60)
          GTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGA                                                                                    <  SRR4302543.438115/94‑1 (MQ=60)
           TACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAG                                                                                   <  SRR4302543.1896910/94‑1 (MQ=60)
           TACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAG                                                                                   <  SRR4302543.7038902/94‑1 (MQ=60)
              CAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATT                                                                                <  SRR4302543.161483/94‑1 (MQ=60)
              CAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATT                                                                                <  SRR4302543.203884/94‑1 (MQ=60)
               AGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTG                                                                               >  SRR4302543.1926574/1‑94 (MQ=60)
                 CATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTG                                                                             >  SRR4302543.3233384/1‑94 (MQ=60)
                 CATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTG                                                                             <  SRR4302543.6056809/94‑1 (MQ=60)
                  ATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGA                                                                            >  SRR4302543.213548/1‑94 (MQ=60)
                   TGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAA                                                                           <  SRR4302543.1921083/94‑1 (MQ=60)
                   TGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAA                                                                           >  SRR4302543.6039108/1‑94 (MQ=60)
                    GAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAAT                                                                          <  SRR4302543.2965301/94‑1 (MQ=60)
                    GAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAAT                                                                          <  SRR4302543.291821/94‑1 (MQ=60)
                     AGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATA                                                                         >  SRR4302543.1159079/1‑94 (MQ=60)
                     AGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGATTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATA                                                                         >  SRR4302543.5565337/1‑94 (MQ=60)
                      GTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATAT                                                                        <  SRR4302543.5647148/94‑1 (MQ=60)
                      GTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATAT                                                                        <  SRR4302543.4108971/94‑1 (MQ=60)
                        AAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCGTGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTG                                                                      <  SRR4302543.5551347/94‑1 (MQ=60)
                        AAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTG                                                                      >  SRR4302543.889753/1‑94 (MQ=60)
                        AAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTG                                                                      >  SRR4302543.299051/1‑94 (MQ=60)
                         AATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGA                                                                     >  SRR4302543.4493130/1‑94 (MQ=60)
                              GCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCC                                                                <  SRR4302543.7649892/94‑1 (MQ=60)
                              GCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGAC                                                                >  SRR4302543.4902439/1‑94 (MQ=60)
                               CCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCG                                                               >  SRR4302543.852327/1‑94 (MQ=60)
                               CCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCG                                                               >  SRR4302543.7237551/1‑94 (MQ=60)
                               CCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCG                                                               <  SRR4302543.6984778/94‑1 (MQ=60)
                               CCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCG                                                               >  SRR4302543.2134436/1‑94 (MQ=60)
                               CCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCG                                                               <  SRR4302543.1243126/94‑1 (MQ=60)
                                CAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGG                                                              >  SRR4302543.7705770/1‑94 (MQ=60)
                                CAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGG                                                              >  SRR4302543.4881479/1‑94 (MQ=60)
                                 AGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGA                                                             >  SRR4302543.1198670/1‑94 (MQ=60)
                                    CGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACC                                                          <  SRR4302543.1297846/94‑1 (MQ=60)
                                    CGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACC                                                          <  SRR4302543.1793465/94‑1 (MQ=60)
                                    CGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACC                                                          >  SRR4302543.3213709/1‑94 (MQ=60)
                                     GTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCG                                                         <  SRR4302543.5714088/94‑1 (MQ=60)
                                     GTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCG                                                         >  SRR4302543.660613/1‑94 (MQ=60)
                                     GTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCG                                                         >  SRR4302543.1036321/1‑94 (MQ=60)
                                          GGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAA                                                    >  SRR4302543.3696267/1‑94 (MQ=60)
                                          GGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAA                                                    <  SRR4302543.5750309/94‑1 (MQ=60)
                                          CGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAA                                                    <  SRR4302543.5811749/94‑1 (MQ=60)
                                            CGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACC                                                  >  SRR4302543.654861/1‑94 (MQ=60)
                                            CGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACC                                                  <  SRR4302543.2555887/94‑1 (MQ=60)
                                            CGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACC                                                  <  SRR4302543.4502310/94‑1 (MQ=60)
                                               TGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCC                                               <  SRR4302543.5638121/94‑1 (MQ=60)
                                                GATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCG                                              >  SRR4302543.4696584/1‑94 (MQ=60)
                                                  TGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCG                                            <  SRR4302543.5218978/94‑1 (MQ=60)
                                                          GCATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGT                                    >  SRR4302543.5155473/1‑94 (MQ=60)
                                                           CATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTT                                   >  SRR4302543.6767135/1‑94 (MQ=60)
                                                           CATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTT                                   <  SRR4302543.2820885/94‑1 (MQ=60)
                                                            ATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTG                                  <  SRR4302543.7664838/94‑1 (MQ=60)
                                                            ATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTG                                  >  SRR4302543.2361939/1‑94 (MQ=60)
                                                            ATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTG                                  >  SRR4302543.7501837/1‑94 (MQ=60)
                                                            ATGCAGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTG                                  >  SRR4302543.6221063/1‑94 (MQ=60)
                                                                AGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCA                              <  SRR4302543.5170129/94‑1 (MQ=60)
                                                                AGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCA                              >  SRR4302543.6193108/1‑94 (MQ=60)
                                                                AGGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCA                              >  SRR4302543.6193375/1‑94 (MQ=60)
                                                                 GGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAG                             <  SRR4302543.4051096/94‑1 (MQ=60)
                                                                 GGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAG                             <  SRR4302543.6760654/94‑1 (MQ=60)
                                                                 GGTTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAACGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAG                             <  SRR4302543.2185804/94‑1 (MQ=60)
                                                                   TTGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCT                           >  SRR4302543.6250476/1‑94 (MQ=60)
                                                                    TGGCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCAT                          <  SRR4302543.6222557/94‑1 (MQ=60)
                                                                      GCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCT                        <  SRR4302543.2489591/94‑1 (MQ=60)
                                                                      GCAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCT                        >  SRR4302543.2967859/1‑94 (MQ=60)
                                                                       CAGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTG                       >  SRR4302543.2054293/1‑94 (MQ=60)
                                                                        AGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGC                      >  SRR4302543.3230947/1‑94 (MQ=60)
                                                                        AGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGC                      >  SRR4302543.6544626/1‑94 (MQ=60)
                                                                        AGCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGC                      >  SRR4302543.6544627/1‑94 (MQ=60)
                                                                         GCGGTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCT                     <  SRR4302543.578015/94‑1 (MQ=60)
                                                                            GTTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTG                  >  SRR4302543.2296262/1‑94 (MQ=60)
                                                                             TTTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGT                 <  SRR4302543.423248/94‑1 (MQ=60)
                                                                              TTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTC                >  SRR4302543.3820699/1‑94 (MQ=60)
                                                                              TTCTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTC                <  SRR4302543.7657947/94‑1 (MQ=60)
                                                                                CTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGA              >  SRR4302543.2580007/1‑94 (MQ=60)
                                                                                CTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGA              >  SRR4302543.6731551/1‑94 (MQ=60)
                                                                                CTACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGA              >  SRR4302543.6731553/1‑94 (MQ=60)
                                                                                 tccccCCACTTACATCGTTTCGCGATTGTGAATATTGAATGCCGGACCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAT             <  SRR4302543.6067381/89‑1 (MQ=60)
                                                                                 TACCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAT             >  SRR4302543.3041555/1‑94 (MQ=60)
                                                                                  TCCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATA            <  SRR4302543.6136324/94‑1 (MQ=60)
                                                                                   CCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATAC           <  SRR4302543.3289357/94‑1 (MQ=60)
                                                                                   CCACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATAC           <  SRR4302543.3165710/94‑1 (MQ=60)
                                                                                    CACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACC          >  SRR4302543.2971973/1‑94 (MQ=60)
                                                                                    CACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACC          <  SRR4302543.6968168/94‑1 (MQ=60)
                                                                                    CACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACC          >  SRR4302543.606089/1‑94 (MQ=60)
                                                                                     CCCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCA         <  SRR4302543.5456246/94‑1 (MQ=60)
                                                                                     ACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCA         >  SRR4302543.2789973/1‑94 (MQ=60)
                                                                                     ACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCA         >  SRR4302543.7020300/1‑94 (MQ=60)
                                                                                     ACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCA         <  SRR4302543.6913223/94‑1 (MQ=60)
                                                                                     ACCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCA         <  SRR4302543.6652846/94‑1 (MQ=60)
                                                                                      CCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCAC        >  SRR4302543.3890065/1‑94 (MQ=60)
                                                                                      CCACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCAC        >  SRR4302543.3890063/1‑94 (MQ=60)
                                                                                        ACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGT      >  SRR4302543.3535231/1‑94 (MQ=60)
                                                                                        ACTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGT      >  SRR4302543.4582326/1‑94 (MQ=60)
                                                                                         CTTACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTA     >  SRR4302543.7948480/1‑94 (MQ=60)
                                                                                           TACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACA   <  SRR4302543.7503450/94‑1 (MQ=60)
                                                                                            ACATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAT  <  SRR4302543.1800786/94‑1 (MQ=60)
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CAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAT  >  NC_000913/2175039‑2175224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑