Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,623	T→C	intergenic (+1270/‑2676)	metG → / → yehI	methionine tRNA synthetase/putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,623	0	T	C	99.6%	860.0 / ‑4.5	269	intergenic (+1270/‑2676)	metG/yehI	methionine tRNA synthetase/putative regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (176/92); minor base G (1/0); total (177/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 272 total aligned reads displayed.

ATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATC  >  NC_000913/2195533‑2195712

atgaGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCAt                                                                                         >  2:3949679/1‑93 (MQ=255)

   aGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTAc                                                                                      <  1:1504753/93‑1 (MQ=255)

   aGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTAc                                                                                      >  2:6625688/1‑93 (MQ=255)

    gCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACa                                                                                     <  1:3409161/93‑1 (MQ=255)

    gCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACa                                                                                     <  1:320587/93‑1 (MQ=255)

     cacaTTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAg                                                                                    >  2:2973838/1‑93 (MQ=255)

            ttGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAg                                                                             >  1:5936630/1‑93 (MQ=255)

                cTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGgtgt                                                                         <  1:1256604/93‑1 (MQ=255)

                cTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGgtgt                                                                         >  2:7158724/1‑93 (MQ=255)

                cTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGgtgt                                                                         <  1:2607696/93‑1 (MQ=255)

                 tCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGgtgct                                                                        <  2:7382353/93‑3 (MQ=255)

                 tCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGtt                                                                        >  1:6856390/1‑93 (MQ=255)

                 tCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGtt                                                                        >  2:1989936/1‑93 (MQ=255)

                 tCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGtt                                                                        >  2:3560994/1‑93 (MQ=255)

                   aTTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGc                                                                      <  1:3131035/93‑1 (MQ=255)

                   aTTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGc                                                                      <  2:4246808/93‑1 (MQ=255)

                    ttAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCa                                                                     <  1:1414236/93‑1 (MQ=255)

                    ttAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCa                                                                     >  1:189883/1‑93 (MQ=255)

                       gAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGcc                                                                  >  2:1228435/1‑93 (MQ=255)

                        aaaaaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGccc                                                                 <  2:5464797/93‑1 (MQ=255)

                        aaaaaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGccc                                                                 >  2:3107688/1‑93 (MQ=255)

                        aaaaaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGccc                                                                 <  2:1839245/93‑1 (MQ=255)

                         aaaaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCt                                                                >  2:4647549/1‑93 (MQ=255)

                         aaaaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCt                                                                >  1:6273614/1‑93 (MQ=255)

                         aaaaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCt                                                                >  2:1721193/1‑93 (MQ=255)

                           aaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGc                                                              >  2:7408932/1‑93 (MQ=255)

                           aaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGc                                                              <  2:1338130/93‑1 (MQ=255)

                           aaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACAGCATTACAGGAGCAAGGTGTTGCAGCCCTGc                                                              >  1:2203612/1‑93 (MQ=255)

                            atatCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGcc                                                             <  1:7923244/93‑1 (MQ=255)

                             tatCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGccc                                                            <  1:704869/93‑1 (MQ=255)

                             catCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGccc                                                            >  2:6312578/2‑93 (MQ=255)

                              atCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGcccc                                                           >  2:797064/1‑93 (MQ=255)

                              atCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGcccc                                                           >  1:35288/1‑93 (MQ=255)

                               tCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCg                                                          <  1:1071389/93‑1 (MQ=255)

                                   gAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGtttt                                                      >  2:5044339/1‑93 (MQ=255)

                                   gAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGAttt                                                      >  1:6880582/1‑93 (MQ=255)

                                     aCCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGc                                                    >  2:6278299/1‑93 (MQ=255)

                                       cATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTc                                                  >  1:6142640/1‑93 (MQ=255)

                                       cATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTc                                                  >  1:2573604/1‑93 (MQ=255)

                                       cATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTc                                                  <  2:4989612/93‑1 (MQ=255)

                                        atatGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTcc                                                 >  2:5877861/1‑93 (MQ=255)

                                        atatGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTcc                                                 >  1:2816098/1‑93 (MQ=255)

                                         tatGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTccc                                                >  2:7547750/1‑93 (MQ=255)

                                         tatGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTccc                                                <  2:6101035/93‑1 (MQ=255)

                                         tatGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTccc                                                >  1:4252635/1‑93 (MQ=255)

                                           tGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTa                                              >  1:1013318/1‑93 (MQ=255)

                                           tGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTa                                              >  2:247712/1‑93 (MQ=255)

                                           tGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTa                                              >  1:5464327/1‑93 (MQ=255)

                                              cATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGc                                           >  2:5731812/1‑93 (MQ=255)

                                              cATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGc                                           >  1:3341953/1‑93 (MQ=255)

                                               aTTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGcc                                          >  2:548889/1‑93 (MQ=255)

                                                tttttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCg                                         >  2:7807391/1‑93 (MQ=255)

                                                tttttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCg                                         >  1:3889057/1‑93 (MQ=255)

                                                tttttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCACTACAGGAGCAAGGGGTTGCGGCCCTGCCCCGCTTTGCTCCCTAAGCCg                                         >  1:1632403/1‑93 (MQ=255)

                                                   ttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCaa                                      >  1:2739136/1‑93 (MQ=255)

                                                   ttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCaa                                      >  1:7327456/1‑93 (MQ=255)

                                                     gatgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAgg                                    >  2:6198136/1‑92 (MQ=255)

                                                     gatgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGt                                    <  2:5956702/93‑1 (MQ=255)

                                                     gatgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGt                                    >  1:2069691/1‑93 (MQ=255)

                                                     gatgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGt                                    <  2:2890126/93‑1 (MQ=255)

                                                     gatgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGt                                    >  2:349435/1‑93 (MQ=255)

                                                      atgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTg                                   >  1:740047/1‑93 (MQ=255)

                                                       tgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGa                                  >  1:5215596/1‑93 (MQ=255)

                                                       tgatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGa                                  >  2:4550497/1‑93 (MQ=255)

                                                        gatTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGac                                 >  2:2266799/1‑93 (MQ=255)

                                                         atTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGact                                >  2:3009411/1‑93 (MQ=255)

                                                           tACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGactac                              >  1:2416604/1‑93 (MQ=255)

                                                            aCTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGactact                             >  2:3281888/1‑93 (MQ=255)

                                                            aCTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGactact                             <  2:4082872/93‑1 (MQ=255)

                                                            aCTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGactact                             <  2:2434236/93‑1 (MQ=255)

                                                            aCTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGactact                             >  2:2174465/1‑93 (MQ=255)

                                                             cTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTg                            >  1:4606121/1‑93 (MQ=255)

                                                              tATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTgc                           >  1:5309839/1‑93 (MQ=255)

                                                              tATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTgc                           <  2:190643/93‑1 (MQ=255)

                                                               aTTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTgcg                          >  2:4372100/1‑93 (MQ=255)

                                                               aTTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGCGACTACTgcg                          >  2:5319033/1‑93 (MQ=255)

                                                                ttGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTgcgc                         >  2:3672974/1‑93 (MQ=255)

                                                                ttGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTgcgc                         >  1:2626861/1‑93 (MQ=255)

                                                                ttGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTgcgc                         >  2:4519503/1‑93 (MQ=255)

                                                                  gtggtgCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCg                       >  1:3702775/1‑93 (MQ=255)

                                                                   tggtgCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGa                      >  1:6404167/1‑93 (MQ=255)

                                                                   tggtgCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGa                      >  2:1775283/1‑93 (MQ=255)

                                                                     gtgCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGAtg                    >  2:6988050/1‑93 (MQ=255)

                                                                        cGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATgtgt                 <  2:2787419/93‑1 (MQ=255)

                                                                          atatGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTg               >  1:4866159/1‑93 (MQ=255)

                                                                          atatGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTg               >  1:291951/1‑93 (MQ=255)

                                                                          atatGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTg               >  1:829899/1‑93 (MQ=255)

                                                                           tatGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGc              <  2:5627173/93‑1 (MQ=255)

                                                                             tGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGt            >  2:2339207/1‑93 (MQ=255)

                                                                             tGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGt            >  2:6737807/1‑93 (MQ=255)

                                                                               gTCAGCCACCGCATTACAGGANCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCa          <  1:4713651/93‑1 (MQ=255)

                                                                                tCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCat         >  1:5579614/1‑93 (MQ=255)

                                                                                   gCCACCGCATTACAGGAGCAAGGTGGTGCACCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTGCTGCGCCGATGTGTTGCGTCATatc      <  1:7457765/93‑1 (MQ=255)

                                                                                    ccACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatca     <  1:5808370/93‑1 (MQ=255)

                                                                                     cACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaa    >  1:7241353/1‑93 (MQ=255)

                                                                                     cACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaa    <  1:43754/93‑1 (MQ=255)

                                                                                      aCCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaat   >  1:6572088/1‑93 (MQ=255)

                                                                                      aCCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaat   <  1:7516390/93‑1 (MQ=255)

                                                                                       ccGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaatc  >  1:7691143/1‑93 (MQ=255)

                                                                                       ccGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaatc  <  1:5711458/93‑1 (MQ=255)

ATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATC  >  NC_000913/2195533‑2195712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  NC_000913/2195530‑2195716

CTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGC                                                                                               >  SRR4302543.243297/1‑94 (MQ=60)

 TGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCA                                                                                              >  SRR4302543.3001216/1‑94 (MQ=60)

 TGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCA                                                                                              <  SRR4302543.5017870/94‑1 (MQ=60)

 TGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCA                                                                                              >  SRR4302543.5476716/1‑94 (MQ=60)

 TGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCA                                                                                              >  SRR4302543.7439395/1‑94 (MQ=60)

   ATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACAGCATT                                                                                            >  SRR4302543.2971245/1‑94 (MQ=60)

    TGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTA                                                                                           >  SRR4302543.7091010/1‑94 (MQ=60)

     GAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTAC                                                                                          >  SRR4302543.1795430/1‑94 (MQ=60)

      AGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACA                                                                                         <  SRR4302543.1518275/94‑1 (MQ=60)

      AGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACA                                                                                         >  SRR4302543.5626041/1‑94 (MQ=60)

       GCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAG                                                                                        <  SRR4302543.4174661/94‑1 (MQ=60)

       GCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAG                                                                                        <  SRR4302543.323486/94‑1 (MQ=60)

       GCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAG                                                                                        <  SRR4302543.3439685/94‑1 (MQ=60)

        CACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGG                                                                                       >  SRR4302543.4384179/1‑94 (MQ=60)

         ACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGA                                                                                      >  SRR4302543.6802447/1‑94 (MQ=60)

          CATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAG                                                                                     <  SRR4302543.3700212/94‑1 (MQ=60)

          CATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAG                                                                                     <  SRR4302543.2863586/94‑1 (MQ=60)

              CTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAG                                                                                 >  SRR4302543.3527805/1‑94 (MQ=60)

              CTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAG                                                                                 >  SRR4302543.7247945/1‑94 (MQ=60)

              CTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAG                                                                                 >  SRR4302543.6596926/1‑94 (MQ=60)

              CTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAG                                                                                 >  SRR4302543.3387536/1‑94 (MQ=60)

              CTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAG                                                                                 <  SRR4302543.5667918/94‑1 (MQ=60)

              CTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAG                                                                                 >  SRR4302543.5990931/1‑94 (MQ=60)

               TTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGG                                                                                >  SRR4302543.4153727/1‑94 (MQ=60)

                TGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGT                                                                               >  SRR4302543.4802668/1‑94 (MQ=60)

                   CTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGaaaggggtg                                                                            >  SRR4302543.2420810/1‑85 (MQ=60)

                   CTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTT                                                                            <  SRR4302543.4367262/94‑1 (MQ=60)

                   CTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTT                                                                            <  SRR4302543.1267857/94‑1 (MQ=60)

                   CTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTT                                                                            <  SRR4302543.2631379/94‑1 (MQ=60)

                   CTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTT                                                                            >  SRR4302543.6921037/1‑94 (MQ=60)

                    TCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTG                                                                           <  SRR4302543.2839120/94‑1 (MQ=60)

                     CATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGC                                                                          >  SRR4302543.59051/1‑94 (MQ=60)

                     CATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGC                                                                          >  SRR4302543.1177387/1‑94 (MQ=60)

                     CATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGC                                                                          >  SRR4302543.1530298/1‑94 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTTAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         >  SRR4302543.1838915/1‑94 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         <  SRR4302543.3159197/94‑1 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         <  SRR4302543.4611565/94‑1 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         <  SRR4302543.7144346/94‑1 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         >  SRR4302543.3157052/1‑94 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         <  SRR4302543.630660/94‑1 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         >  SRR4302543.191493/1‑94 (MQ=60)

                      ATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCA                                                                         <  SRR4302543.3546547/94‑1 (MQ=60)

                       TTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAG                                                                        <  SRR4302543.1427036/94‑1 (MQ=60)

                        TAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGC                                                                       >  SRR4302543.5368425/1‑94 (MQ=60)

                        TAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGC                                                                       >  SRR4302543.7528289/1‑94 (MQ=60)

                          GAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCC                                                                     >  SRR4302543.584274/1‑94 (MQ=60)

                          GAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCC                                                                     >  SRR4302543.7560188/1‑94 (MQ=60)

                           AAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCT                                                                    >  SRR4302543.5355831/1‑94 (MQ=60)

                           AAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCT                                                                    <  SRR4302543.1333480/94‑1 (MQ=60)

                           AAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCT                                                                    >  SRR4302543.6332760/1‑94 (MQ=60)

                             AAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACAGCATTACAGGAGCAAGGTGTTGCAGCCCTGC                                                                  >  SRR4302543.2223443/1‑94 (MQ=60)

                              AATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCC                                                                 <  SRR4302543.323081/94‑1 (MQ=60)

                               ATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCC                                                                <  SRR4302543.7997662/94‑1 (MQ=60)

                                TATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCC                                                               <  SRR4302543.711294/94‑1 (MQ=60)

                                TATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCC                                                               >  SRR4302543.35611/1‑94 (MQ=60)

                                 ATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCG                                                              >  SRR4302543.1386063/1‑94 (MQ=60)

                                  TCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGA                                                             <  SRR4302543.1081020/94‑1 (MQ=60)

                                    ACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATT                                                           <  SRR4302543.796681/94‑1 (MQ=60)

                                    ACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATT                                                           >  SRR4302543.6387793/1‑94 (MQ=60)

                                     CGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTT                                                          >  SRR4302543.6800034/1‑94 (MQ=60)

                                     CGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTT                                                          >  SRR4302543.6945326/1‑94 (MQ=60)

                                      GAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTG                                                         >  SRR4302543.833336/1‑94 (MQ=60)

                                      GAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTG                                                         <  SRR4302543.6499129/94‑1 (MQ=60)

                                      CAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTG                                                         <  SRR4302543.2613289/94‑1 (MQ=60)

                                       AACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGC                                                        >  SRR4302543.7034816/1‑94 (MQ=60)

                                        ACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCT                                                       >  SRR4302543.6054543/1‑94 (MQ=60)

                                         CCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTC                                                      >  SRR4302543.6200818/1‑94 (MQ=60)

                                         CCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTC                                                      >  SRR4302543.2597068/1‑94 (MQ=60)

                                         CCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTC                                                      >  SRR4302543.1100712/1‑94 (MQ=60)

                                          CATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCC                                                     >  SRR4302543.2841884/1‑94 (MQ=60)

                                           ATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCC                                                    <  SRR4302543.2633182/94‑1 (MQ=60)

                                           ATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCC                                                    >  SRR4302543.4291241/1‑94 (MQ=60)

                                             ATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTA                                                  >  SRR4302543.5513846/1‑94 (MQ=60)

                                             ATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTA                                                  <  SRR4302543.3224984/94‑1 (MQ=60)

                                             ATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTA                                                  >  SRR4302543.1022285/1‑94 (MQ=60)

                                             ATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTA                                                  >  SRR4302543.7557610/1‑94 (MQ=60)

                                             ATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTA                                                  >  SRR4302543.7435805/1‑94 (MQ=60)

                                              TGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTGCAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTAT                                                 <  SRR4302543.2805971/94‑1 (MQ=60)

                                              TGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTAT                                                 <  SRR4302543.4891221/94‑1 (MQ=60)

                                              TGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTAT                                                 <  SRR4302543.595653/94‑1 (MQ=60)

                                              TGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTAT                                                 >  SRR4302543.3841996/1‑94 (MQ=60)

                                                CCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGC                                               >  SRR4302543.3688442/1‑94 (MQ=60)

                                                CCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGC                                               >  SRR4302543.3372051/1‑94 (MQ=60)

                                                CCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGC                                               >  SRR4302543.2665398/1‑94 (MQ=60)

                                                 CATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCC                                              >  SRR4302543.4455474/1‑94 (MQ=60)

                                                  ATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCG                                             >  SRR4302543.3923393/1‑94 (MQ=60)

                                                  ATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCG                                             >  SRR4302543.7896352/1‑94 (MQ=60)

                                                  ATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCG                                             >  SRR4302543.7391621/1‑94 (MQ=60)

                                                  ATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCACTACAGGAGCAAGGGGTTGCGGCCCTGCCCCGCTTTGCTCCCTAAGCCG                                             >  SRR4302543.1646957/1‑94 (MQ=60)

                                                     TTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAA                                          >  SRR4302543.2764054/1‑94 (MQ=60)

                                                     TTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAA                                          >  SRR4302543.7397997/1‑94 (MQ=60)

                                                       TGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGT                                        >  SRR4302543.7496118/1‑94 (MQ=60)

                                                       TGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGT                                        >  SRR4302543.2513688/1‑94 (MQ=60)

                                                       TGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGT                                        <  SRR4302543.6936159/94‑1 (MQ=60)

                                                       TGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGT                                        >  SRR4302543.2088186/1‑94 (MQ=60)

                                                        GATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTG                                       >  SRR4302543.746661/1‑94 (MQ=60)

                                                         ATGATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGA                                      >  SRR4302543.5262583/1‑94 (MQ=60)

                                                           GATTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACT                                    <  SRR4302543.2585073/94‑1 (MQ=60)

                                                             TTACTATTGTGGTGCGATATGGTCAGCCTCCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTAC                                  <  SRR4302543.3379522/94‑1 (MQ=60)

                                                             TTACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTAC                                  >  SRR4302543.2438433/1‑94 (MQ=60)

                                                              TACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACT                                 <  SRR4302543.1047629/94‑1 (MQ=60)

                                                              TACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACT                                 <  SRR4302543.2201591/94‑1 (MQ=60)

                                                               ACTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTG                                >  SRR4302543.4647963/1‑94 (MQ=60)

                                                                CTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGC                               >  SRR4302543.2051008/1‑94 (MQ=60)

                                                                CTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGC                               >  SRR4302543.827452/1‑94 (MQ=60)

                                                                CTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGC                               >  SRR4302543.5357797/1‑94 (MQ=60)

                                                                CTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGC                               >  SRR4302543.5072223/1‑94 (MQ=60)

                                                                CTATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGC                               >  SRR4302543.2708479/1‑94 (MQ=60)

                                                                  ATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGC                             >  SRR4302543.2371989/1‑94 (MQ=60)

                                                                  ATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGC                             >  SRR4302543.2650634/1‑94 (MQ=60)

                                                                  ATTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGC                             >  SRR4302543.1610862/1‑94 (MQ=60)

                                                                   TTGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCC                            <  SRR4302543.574951/94‑1 (MQ=60)

                                                                    TGTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCG                           >  SRR4302543.3735327/1‑94 (MQ=60)

                                                                     GTGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGA                          >  SRR4302543.6464476/1‑94 (MQ=60)

                                                                      TGGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGAT                         <  SRR4302543.1516139/94‑1 (MQ=60)

                                                                       GGTGCGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATG                        >  SRR4302543.7612850/1‑94 (MQ=60)

                                                                           CGATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTT                    >  SRR4302543.327126/1‑94 (MQ=60)

                                                                            GATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTG                   >  SRR4302543.4910517/1‑94 (MQ=60)

                                                                            GATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTG                   >  SRR4302543.837213/1‑94 (MQ=60)

                                                                            GATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTG                   >  SRR4302543.294562/1‑94 (MQ=60)

                                                                             ATATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGC                  >  SRR4302543.7454159/1‑94 (MQ=60)

                                                                              TATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTTCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCG                 <  SRR4302543.4711096/94‑1 (MQ=60)

                                                                              TATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCG                 >  SRR4302543.5181087/1‑94 (MQ=60)

                                                                              TATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCG                 <  SRR4302543.6379881/94‑1 (MQ=60)

                                                                              TATGGTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCG                 <  SRR4302543.6143987/94‑1 (MQ=60)

                                                                                  GTCAGCCACCGCATTACAGGANCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCAT             <  SRR4302543.4756723/94‑1 (MQ=60)

                                                                                  GTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCAT             >  SRR4302543.5625641/1‑94 (MQ=60)

                                                                                  GTCAGCCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCAT             >  SRR4302543.5630409/1‑94 (MQ=60)

                                                                                   TCAGCCACCGCATTACAGGAGCAAGTTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATA            <  SRR4302543.5485395/94‑1 (MQ=60)

                                                                                      GCCACCGCATTACAGGAGCAAGGTGGTGCACCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTGCTGCGCCGATGTGTTGCGTCATATCA         <  SRR4302543.7529206/94‑1 (MQ=60)

                                                                                       CCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAA        >  SRR4302543.7311439/1‑94 (MQ=60)

                                                                                       CCACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAA        <  SRR4302543.5861196/94‑1 (MQ=60)

                                                                                        CACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAAT       <  SRR4302543.44157/94‑1 (MQ=60)

                                                                                        CACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAAT       >  SRR4302543.6633960/1‑94 (MQ=60)

                                                                                        CACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAAT       >  SRR4302543.2644498/1‑94 (MQ=60)

                                                                                        CACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAAT       >  SRR4302543.2075382/1‑94 (MQ=60)

                                                                                         ACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATC      <  SRR4302543.7588159/94‑1 (MQ=60)

                                                                                         ACCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATC      >  SRR4302543.7763961/1‑94 (MQ=60)

                                                                                          CCGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCA     <  SRR4302543.5763565/94‑1 (MQ=60)

                                                                                           CGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCAT    <  SRR4302543.945606/94‑1 (MQ=60)

                                                                                           CGCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCAC    >  SRR4302543.6084516/1‑94 (MQ=60)

                                                                                            GCATTACAGGAGCAAGGTTTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATC   <  SRR4302543.3005678/94‑1 (MQ=60)

                                                                                            GCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATC   <  SRR4302543.3005676/94‑1 (MQ=60)

                                                                                            GCATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATC   >  SRR4302543.7263816/1‑94 (MQ=60)

                                                                                             CATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  SRR4302543.7716553/1‑94 (MQ=60)

                                                                                             CATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  SRR4302543.7270704/1‑94 (MQ=60)

                                                                                             CATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  SRR4302543.1549284/1‑94 (MQ=60)

                                                                                             CATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  SRR4302543.3093954/1‑94 (MQ=60)

                                                                                             CATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  SRR4302543.4224148/1‑94 (MQ=60)

                                                                                             CATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  SRR4302543.4493803/1‑94 (MQ=60)

CTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCC  >  NC_000913/2195530‑2195716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`