Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,331 A→G S404S (TCA→TCG)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,3310AG99.4% 533.0 / ‑5.6 175S404S (TCA→TCG) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (60/114);  total (60/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 197 total aligned reads displayed.

GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420
                                                                                          |                                                                                         
 ccGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCaa                                                                                        <  2:786041/93‑1 (MQ=255)
 ccGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCaa                                                                                        <  2:4793090/93‑1 (MQ=255)
   gctaaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagc                                                                                      <  2:3834926/90‑1 (MQ=255)
   gcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCCAAATGTTTTGCAagg                                                                                      >  2:7428636/1‑92 (MQ=255)
    cgAAGTGAACAGGAAAGCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagca                                                                                     <  2:6327061/93‑1 (MQ=255)
    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagca                                                                                     <  2:135204/93‑1 (MQ=255)
     gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAagcag                                                                                    >  1:7705176/1‑93 (MQ=255)
       aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTGTTTTGCAAgcagca                                                                                  >  1:4216474/1‑93 (MQ=255)
       aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAgcagca                                                                                  <  2:3641363/93‑1 (MQ=255)
        gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCaa                                                                                 <  2:3151288/93‑1 (MQ=255)
        gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCaa                                                                                 <  1:2854846/93‑1 (MQ=255)
           aaCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAc                                                                              <  1:1190859/93‑1 (MQ=255)
           aaCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAc                                                                              <  1:5509386/93‑1 (MQ=255)
           aaCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAc                                                                              <  1:3330335/93‑1 (MQ=255)
            aCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACg                                                                             <  2:3274526/93‑1 (MQ=255)
             cAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGc                                                                            <  1:1495184/93‑1 (MQ=255)
             cAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGc                                                                            <  1:505685/93‑1 (MQ=255)
              aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  2:4780715/93‑1 (MQ=255)
              aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  2:2952064/93‑1 (MQ=255)
               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAAAAAGCAg                                                                          >  2:7274232/1‑93 (MQ=255)
                gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  1:5714600/93‑1 (MQ=255)
                gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  2:2748766/93‑1 (MQ=255)
                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCCGGCCAATTGTTTTGCAAGCAGCAAAAAGCAGTgtc                                                                      >  1:1699511/1‑91 (MQ=255)
                     cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:5556723/93‑1 (MQ=255)
                     cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:1733058/93‑1 (MQ=255)
                     cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:252546/93‑1 (MQ=255)
                     cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:6774928/93‑1 (MQ=255)
                       cACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATg                                                                  <  2:1783750/93‑1 (MQ=255)
                        cccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  1:3101974/92‑1 (MQ=255)
                         ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGc                                                                <  1:4929930/93‑1 (MQ=255)
                           ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGa                                                              <  2:7602537/93‑1 (MQ=255)
                              aGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCCAAACGCAGCGCCGCTGGCCAgct                                                           >  2:5003537/1‑93 (MQ=255)
                              aGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgct                                                           <  1:6515160/93‑1 (MQ=255)
                               gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctg                                                          >  2:6704204/1‑93 (MQ=255)
                                cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgc                                                         <  1:7586685/93‑1 (MQ=255)
                                  aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCCATGGCGAGctgctg                                                       >  2:1644886/1‑93 (MQ=255)
                                     cgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTa                                                    <  2:5593274/93‑1 (MQ=255)
                                     cgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTa                                                    <  2:5701999/93‑1 (MQ=255)
                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   >  1:3693139/1‑93 (MQ=255)
                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   >  2:2654036/1‑93 (MQ=255)
                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   >  2:5885055/1‑93 (MQ=255)
                                       tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGc                                                  <  1:7792840/93‑1 (MQ=255)
                                        cGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGTTAGCt                                                 >  2:3113270/1‑93 (MQ=255)
                                         ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                <  2:5751048/93‑1 (MQ=255)
                                          gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               >  1:3510276/1‑93 (MQ=255)
                                          gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               <  1:2117961/93‑1 (MQ=255)
                                          gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               >  1:5469359/1‑93 (MQ=255)
                                          gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               <  2:290283/93‑1 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGGAGTGCCGATGGCGAGCCGCTGTTAGCTAcc                                              >  1:4647864/1‑93 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  1:3932540/1‑93 (MQ=255)
                                            tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             <  1:3279368/93‑1 (MQ=255)
                                            tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             >  1:3977436/1‑93 (MQ=255)
                                             gTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGATGTTAGCTACCCg                                            >  1:7866077/1‑93 (MQ=255)
                                              tttAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAAATGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGc                                           >  2:861004/1‑93 (MQ=255)
                                              tttAGTTTTCAGGCTGGCGCACTAGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGc                                           <  2:5651895/93‑1 (MQ=255)
                                                tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  2:1863041/93‑1 (MQ=255)
                                                tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  2:3510170/93‑1 (MQ=255)
                                                tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         >  2:317708/1‑93 (MQ=255)
                                                  gTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAcc                                       >  2:4194424/1‑93 (MQ=255)
                                                    tttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     <  1:6827697/93‑1 (MQ=255)
                                                       cAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTa                                  <  2:3383600/93‑1 (MQ=255)
                                                        aGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTaa                                 >  2:7120909/1‑93 (MQ=255)
                                                           cTGGCGCACTGGCGGGCGGGAAAATTGTTTCGCAAGCAGCAAATCGCAGTGCCGATGGCGCGCTGCTGTTAGCTACCCGCAACCGCTTAagca                              <  2:4604131/93‑1 (MQ=255)
                                                            gggCGCACTGGGGGGCGGGAAAATGGTTTCGCAAGCAGCAAACCGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAGCCGCTTAagcag                             <  2:7363548/92‑1 (MQ=255)
                                                             ggCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  2:7869578/93‑1 (MQ=255)
                                                             ggCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  2:4130289/93‑1 (MQ=255)
                                                              gcgcACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCg                           >  1:2701266/1‑93 (MQ=255)
                                                               ggcACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  1:1569286/92‑1 (MQ=255)
                                                                gcACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  1:537983/93‑1 (MQ=255)
                                                                 cACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCGGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtg                        >  2:4670984/1‑93 (MQ=255)
                                                                 cACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtg                        >  2:6225548/1‑93 (MQ=255)
                                                                 cACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtg                        >  1:2398267/1‑93 (MQ=255)
                                                                  aCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgt                       >  1:5901463/1‑93 (MQ=255)
                                                                   cTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGAGAGCTGCTGTTTGCTACCCGCAACAGCTTAAGCAGCGCtgtg                      >  2:311187/1‑93 (MQ=255)
                                                                    tGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     <  2:5362259/93‑1 (MQ=255)
                                                                       cgggcgggCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  >  2:3666609/1‑93 (MQ=255)
                                                                       cgggcgggCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  <  1:7241231/93‑1 (MQ=255)
                                                                       cgggcgggCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  <  2:3542687/93‑1 (MQ=255)
                                                                        gggcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCt                 >  2:2577283/1‑93 (MQ=255)
                                                                         ggcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                >  1:7467837/1‑93 (MQ=255)
                                                                         ggcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                <  2:3038460/93‑1 (MQ=255)
                                                                              gcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGcc           >  1:2916570/1‑93 (MQ=255)
                                                                               cAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          <  2:5037587/93‑1 (MQ=255)
                                                                               cAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          <  1:4768560/93‑1 (MQ=255)
                                                                                aaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  1:4953723/1‑93 (MQ=255)
                                                                                 aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:6099385/93‑1 (MQ=255)
                                                                                 aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:4295615/93‑1 (MQ=255)
                                                                                 aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  2:1146294/93‑1 (MQ=255)
                                                                                 aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  2:1535491/93‑1 (MQ=255)
                                                                                  aTTGTTTCGCAAGCAGCAAAATGCATTGCCGATGGCGAGCTGCTGGTAACTACCCGCAACCGCTTAAGCAGCGGTGTGCCGCTGTCGCCTGAt       >  2:7048071/1‑93 (MQ=255)
                                                                                   ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      >  1:3019049/1‑93 (MQ=255)
                                                                                   ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      >  1:6300759/1‑93 (MQ=255)
                                                                                    tGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     >  1:2367804/1‑93 (MQ=255)
                                                                                    tGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     >  1:6748057/1‑93 (MQ=255)
                                                                                     gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:5786909/93‑1 (MQ=255)
                                                                                     gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:4885754/93‑1 (MQ=255)
                                                                                     gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  1:6592259/93‑1 (MQ=255)
                                                                                      tttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCt   >  1:1002487/1‑93 (MQ=255)
                                                                                       ttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  2:6039319/93‑1 (MQ=255)
                                                                                       ttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  2:5370580/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208238‑2208423
                                                                                             |                                                                                            
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCG                                                                                              <  SRR4302543.1187531/94‑1 (MQ=60)
 TGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGGGGGCAAAATGTTTCGC                                                                                             >  SRR4302543.3080531/1‑94 (MQ=60)
 TGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGC                                                                                             >  SRR4302543.5609048/1‑94 (MQ=60)
 TGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGC                                                                                             >  SRR4302543.5609047/1‑94 (MQ=60)
       CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAG                                                                                       >  SRR4302543.7778075/1‑94 (MQ=60)
       CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAG                                                                                       <  SRR4302543.7500583/94‑1 (MQ=60)
       CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAG                                                                                       <  SRR4302543.1748238/94‑1 (MQ=60)
         AAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTGTTTTGCAAGCAGCA                                                                                     >  SRR4302543.4254703/1‑94 (MQ=60)
          AGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAA                                                                                    >  SRR4302543.4236975/1‑94 (MQ=60)
          AGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAA                                                                                    <  SRR4302543.5907863/94‑1 (MQ=60)
           GTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAA                                                                                   <  SRR4302543.2880891/94‑1 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAC                                                                                 <  SRR4302543.4703278/94‑1 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAC                                                                                 <  SRR4302543.4988719/94‑1 (MQ=60)
              AACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTGTTTCCCCAGCAGCAAAACG                                                                                >  SRR4302543.1526401/1‑94 (MQ=60)
              AACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACG                                                                                <  SRR4302543.5559403/94‑1 (MQ=60)
              AACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACG                                                                                <  SRR4302543.3360366/94‑1 (MQ=60)
              AACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACG                                                                                <  SRR4302543.1201599/94‑1 (MQ=60)
                CAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCA                                                                              <  SRR4302543.510382/94‑1 (MQ=60)
                CAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCA                                                                              <  SRR4302543.1508634/94‑1 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             >  SRR4302543.5586266/1‑94 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAG                                                                             <  SRR4302543.6369669/94‑1 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           <  SRR4302543.939153/94‑1 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           <  SRR4302543.5766740/94‑1 (MQ=60)
                     AAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCGAATTGTTTCGCAAGCAGCAAAACGCAGTGCC                                                                         >  SRR4302543.6980675/1‑94 (MQ=60)
                     AAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCCGGCCAATTGTTTTGCAAGCAGCAAAAAGCAGTGTC                                                                         >  SRR4302543.1714742/1‑94 (MQ=60)
                      AACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCG                                                                        <  SRR4302543.3082438/94‑1 (MQ=60)
                      AACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCG                                                                        <  SRR4302543.5032835/94‑1 (MQ=60)
                        CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAT                                                                      <  SRR4302543.6838930/94‑1 (MQ=60)
                        CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAT                                                                      <  SRR4302543.1748503/94‑1 (MQ=60)
                        CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAT                                                                      <  SRR4302543.5607263/94‑1 (MQ=60)
                        CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAT                                                                      <  SRR4302543.254748/94‑1 (MQ=60)
                           CCCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGC                                                                   <  SRR4302543.3129872/94‑1 (MQ=60)
                           ACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGC                                                                   <  SRR4302543.1406355/94‑1 (MQ=60)
                           ACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCCATGGC                                                                   >  SRR4302543.5302656/1‑94 (MQ=60)
                            CCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCG                                                                  <  SRR4302543.4618719/94‑1 (MQ=60)
                            CCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCG                                                                  <  SRR4302543.4974838/94‑1 (MQ=60)
                                 AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTG                                                             <  SRR4302543.6576525/94‑1 (MQ=60)
                                   CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                           <  SRR4302543.7658777/94‑1 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      >  SRR4302543.3725602/1‑94 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      <  SRR4302543.2612308/94‑1 (MQ=60)
                                         GTCGGCTGTTTAGTTTTCAGGCTGGAGCACTGGCGGGCGGGCAAATTGTTTCGCAAGAAGCCAAACGCAGTGCCGAgcgagagatgctgtttgc                                                     >  SRR4302543.6854973/1‑76 (MQ=60)
                                          TCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCT                                                    <  SRR4302543.7866283/94‑1 (MQ=60)
                                            GGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAC                                                  >  SRR4302543.3541645/1‑94 (MQ=60)
                                            GGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAC                                                  >  SRR4302543.5518897/1‑94 (MQ=60)
                                             GCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGGAGTGCCGATGGCGAGCCGCTGTTAGCTACC                                                 >  SRR4302543.4690152/1‑94 (MQ=60)
                                             GCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACC                                                 <  SRR4302543.2136922/94‑1 (MQ=60)
                                             GCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACC                                                 >  SRR4302543.3967273/1‑94 (MQ=60)
                                             GCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACC                                                 >  SRR4302543.7816373/1‑94 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                <  SRR4302543.2840082/94‑1 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                >  SRR4302543.2485045/1‑94 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                >  SRR4302543.4012707/1‑94 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                >  SRR4302543.7674386/1‑94 (MQ=60)
                                               TGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCG                                               <  SRR4302543.3308975/94‑1 (MQ=60)
                                               TGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGATGTTAGCTACCCG                                               >  SRR4302543.7940150/1‑94 (MQ=60)
                                                GTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGC                                              >  SRR4302543.6816297/1‑94 (MQ=60)
                                                      TTTTCAGGGTGGCGCGCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGC                                        <  SRR4302543.6654994/94‑1 (MQ=60)
                                                       TTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       <  SRR4302543.6892154/94‑1 (MQ=60)
                                                       TTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       >  SRR4302543.3345457/1‑94 (MQ=60)
                                                        TTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTT                                      >  SRR4302543.6338735/1‑94 (MQ=60)
                                                             GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 >  SRR4302543.1770953/1‑94 (MQ=60)
                                                             GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302543.3137968/94‑1 (MQ=60)
                                                                GGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCG                              >  SRR4302543.2725768/1‑94 (MQ=60)
                                                                GGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCG                              >  SRR4302543.2417507/1‑94 (MQ=60)
                                                                  GGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTT                            <  SRR4302543.1583298/94‑1 (MQ=60)
                                                                  CGCACGGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTT                            <  SRR4302543.5651031/94‑1 (MQ=60)
                                                                   GCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTTCCCATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGCTG                           >  SRR4302543.4426353/1‑94 (MQ=60)
                                                                   GCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTG                           >  SRR4302543.2419960/1‑94 (MQ=60)
                                                                   GCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTG                           <  SRR4302543.542967/94‑1 (MQ=60)
                                                                    CACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGT                          >  SRR4302543.5955302/1‑94 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302543.3423949/94‑1 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                         <  SRR4302543.167151/94‑1 (MQ=60)
                                                                       TGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCC                       <  SRR4302543.755541/94‑1 (MQ=60)
                                                                          CGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCT                    <  SRR4302543.7311316/94‑1 (MQ=60)
                                                                          CGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCT                    >  SRR4302543.2143983/1‑94 (MQ=60)
                                                                           GGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTG                   >  SRR4302543.7539358/1‑94 (MQ=60)
                                                                                GGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCC              >  SRR4302543.2943070/1‑94 (MQ=60)
                                                                                 GCAAATTGTTTCGCAAGCAGCAAACCGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCT             <  SRR4302543.3000185/94‑1 (MQ=60)
                                                                                 GCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCT             <  SRR4302543.5548586/94‑1 (MQ=60)
                                                                                  CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            >  SRR4302543.36992/1‑94 (MQ=60)
                                                                                  CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            <  SRR4302543.4812144/94‑1 (MQ=60)
                                                                                  CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            >  SRR4302543.4998833/1‑94 (MQ=60)
                                                                                  CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            <  SRR4302543.2900503/94‑1 (MQ=60)
                                                                                    AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302543.4334660/94‑1 (MQ=60)
                                                                                    AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302543.6157175/94‑1 (MQ=60)
                                                                                     ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         >  SRR4302543.6360051/1‑94 (MQ=60)
                                                                                     ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         <  SRR4302543.4269735/94‑1 (MQ=60)
                                                                                     ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         >  SRR4302543.3046350/1‑94 (MQ=60)
                                                                                      TTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGC        >  SRR4302543.6811777/1‑94 (MQ=60)
                                                                                      TTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGC        >  SRR4302543.490613/1‑94 (MQ=60)
                                                                                      TTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGC        >  SRR4302543.2389274/1‑94 (MQ=60)
                                                                                       TGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCC       <  SRR4302543.3841670/94‑1 (MQ=60)
                                                                                       TGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCC       <  SRR4302543.4000442/94‑1 (MQ=60)
                                                                                       TGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCC       <  SRR4302543.6631584/94‑1 (MQ=60)
                                                                                        GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      <  SRR4302543.6654371/94‑1 (MQ=60)
                                                                                        GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      >  SRR4302543.7894325/1‑94 (MQ=60)
                                                                                        GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      >  SRR4302543.1011347/1‑94 (MQ=60)
                                                                                          TTCGCAAGGAGAAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    <  SRR4302543.2268795/94‑1 (MQ=60)
                                                                                           TCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGC   <  SRR4302543.7986922/94‑1 (MQ=60)
                                                                                            CGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  SRR4302543.7062702/1‑94 (MQ=60)
                                                                                            CGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  SRR4302543.4295550/1‑94 (MQ=60)
                                                                                            CGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  SRR4302543.2315181/1‑94 (MQ=60)
                                                                                             |                                                                                            
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208238‑2208423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑