Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,331 A→G S404S (TCA→TCG)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,3310AG99.1% 351.4 / NA 116S404S (TCA→TCG) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (35/80);  minor base C (1/0);  total (36/80)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑ACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420
                                                                                            |                                                                                         
gCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTc‑gca                                                                                         <  2:3784379/93‑1 (MQ=255)
 ccGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCaa                                                                                        <  1:629900/93‑1 (MQ=255)
   gcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagc                                                                                      <  2:4292371/93‑1 (MQ=255)
   gcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTCC‑GCAagc                                                                                      <  2:4455420/93‑1 (MQ=255)
    cgAAGTGAACAGGAAAACTCACCGGCAGCTACTCGTCGTCTGTTTAGGTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagca                                                                                     <  1:2935553/93‑1 (MQ=255)
    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAAATTGTTTT‑GCAagca                                                                                     >  2:751080/1‑93 (MQ=255)
    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagca                                                                                     <  1:2022302/93‑1 (MQ=255)
    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagca                                                                                     <  1:3267067/93‑1 (MQ=255)
     gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagcag                                                                                    <  1:2368330/93‑1 (MQ=255)
      aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAAATTTTTTC‑CCaacagc                                                                                    >  2:3193569/1‑89 (MQ=255)
      aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAagcagc                                                                                   <  2:917683/93‑1 (MQ=255)
       aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTT‑GCAAgcagca                                                                                  >  2:1373715/1‑93 (MQ=255)
        gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGGaa                                                                                 >  1:3872328/1‑93 (MQ=255)
        gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCaa                                                                                 >  2:4470262/1‑93 (MQ=255)
          gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCaaaa                                                                               <  1:269910/93‑1 (MQ=255)
          gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCaaaa                                                                               <  1:3378288/93‑1 (MQ=255)
            aCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACg                                                                             <  1:4038233/93‑1 (MQ=255)
              aagAAAACCCACCGGCAGCTACGCATCGGCTGTTGAGTGTTCAGACTGGCGCACGGGCGTGC‑GAGCAAATTGTTTC‑GCAAGCAGCGAAACGCa                                                                           <  2:732170/91‑1 (MQ=255)
               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAg                                                                          <  1:3438604/93‑1 (MQ=255)
               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAg                                                                          <  1:3225860/93‑1 (MQ=255)
               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAg                                                                          <  1:2805914/93‑1 (MQ=255)
                gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGt                                                                         <  1:1214976/93‑1 (MQ=255)
                 aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAACATGTTTT‑GTAAAGAGCAAAACGCAGTg                                                                        >  1:3195368/1‑93 (MQ=255)
                 aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTg                                                                        <  2:2691550/93‑1 (MQ=255)
                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCCAATTGTTTC‑GCAAACAGCAAAACGCAGTGcc                                                                      >  1:22067/1‑93 (MQ=255)
                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGcc                                                                      >  1:1274520/1‑93 (MQ=255)
                     cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:2297436/93‑1 (MQ=255)
                      tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTT‑GCCAGCAGCAAAACGCAGTGCCGAt                                                                   >  2:1417216/1‑93 (MQ=255)
                        gctggCAGCTCCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  2:2805621/90‑1 (MQ=255)
                        cccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  1:866627/92‑1 (MQ=255)
                        cccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  2:1286749/92‑1 (MQ=255)
                        aCCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATgg                                                                 <  1:1524041/93‑1 (MQ=255)
                         ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGG‑GGGCAAATTGTTTT‑GCAAGCAGCAAAACGCAGTGGCGATggg                                                                >  2:1640396/1‑92 (MQ=255)
                         ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTT‑GCAAGCAGCAAAAAGCAGTGCCGATggg                                                                >  2:4138485/1‑92 (MQ=255)
                          cGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGACGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCg                                                               <  2:4640549/93‑1 (MQ=255)
                           ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCgg                                                              >  2:3551857/1‑92 (MQ=255)
                             cAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgc                                                            <  2:303819/93‑1 (MQ=255)
                              aGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCCATGGCGAgct                                                           >  2:3184001/1‑93 (MQ=255)
                               gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCCGTGCCGATGGCGAgctg                                                          >  1:707997/1‑93 (MQ=255)
                                cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgc                                                         <  1:3230981/93‑1 (MQ=255)
                                cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgc                                                         <  2:247949/93‑1 (MQ=255)
                                cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgc                                                         <  1:1471197/93‑1 (MQ=255)
                                 tACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgct                                                        <  1:1140134/93‑1 (MQ=255)
                                   ggcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑TGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGt                                                      <  1:2656660/92‑1 (MQ=255)
                                     cgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTa                                                    <  1:4495365/93‑1 (MQ=255)
                                     cgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTa                                                    <  1:311136/93‑1 (MQ=255)
                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   <  2:229377/93‑1 (MQ=255)
                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   >  1:2172422/1‑93 (MQ=255)
                                       tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGGAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGc                                                  >  1:5023/1‑93 (MQ=255)
                                       tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGc                                                  <  1:2563544/93‑1 (MQ=255)
                                         ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                <  1:850210/93‑1 (MQ=255)
                                         ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                >  1:997033/1‑93 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCCAATTGGTTA‑CCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:3915240/1‑93 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  1:1219005/93‑1 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:1308273/1‑93 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  2:2769766/93‑1 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGACAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  2:1508277/93‑1 (MQ=255)
                                            tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             >  2:3164409/1‑93 (MQ=255)
                                               ttAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCa                                          >  2:3083621/1‑93 (MQ=255)
                                               ttAGTTTTCAGGCTGGCGCACTGGCGGGC‑CGGCAAATTGATTCGAAAACCACAAAACCGCCGGCCAGAT‑GCGGGCTGCTGTTAGCTACCCGCa                                          >  2:3135342/1‑93 (MQ=255)
                                                tAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  2:3717514/93‑1 (MQ=255)
                                                  gTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGGAGTGCCGATTGCGAGCTGCTGTTAGTTACCCGCAAcc                                       >  1:3557420/1‑93 (MQ=255)
                                                  gTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAcc                                       <  1:4314166/93‑1 (MQ=255)
                                                     ttCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    <  1:2825624/93‑1 (MQ=255)
                                                      tCAGGCTGGCGCACAGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGCGCCGCCGGTTGCTACCCGGACCCGCtt                                   >  2:4283109/1‑93 (MQ=255)
                                                          gCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  1:465137/93‑1 (MQ=255)
                                                          gCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  1:2465331/93‑1 (MQ=255)
                                                          gCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               >  1:2736957/1‑93 (MQ=255)
                                                            tGGCGCAGTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcag                             >  2:4608586/1‑93 (MQ=255)
                                                            tGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcag                             <  1:4098366/93‑1 (MQ=255)
                                                               cgcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:2899915/93‑1 (MQ=255)
                                                               cgcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:293550/93‑1 (MQ=255)
                                                                gcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  2:3728899/93‑1 (MQ=255)
                                                                gcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  1:636751/93‑1 (MQ=255)
                                                                gcACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         <  2:1230295/93‑1 (MQ=255)
                                                                 cACTGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCACTGCCGATGGCGAGCTGCCGGTAGGTACCCGCAACCGCCTGAGCAGCGTtg                        >  2:3607591/1‑93 (MQ=255)
                                                                    tGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACGCGCAACCGCTTAAGCAGCGTTGTGc                     >  2:3512950/1‑93 (MQ=255)
                                                                    tGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     >  2:3711151/1‑93 (MQ=255)
                                                                    tGGCGGGC‑GGGCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     <  1:3790625/93‑1 (MQ=255)
                                                                       cgggc‑gggCAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  <  1:2194222/93‑1 (MQ=255)
                                                                          gc‑gggcAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGt               <  1:776362/93‑1 (MQ=255)
                                                                           c‑gggcAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTc              <  2:2107240/93‑1 (MQ=255)
                                                                              ggcAAATTTTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGc            <  2:2585556/93‑1 (MQ=255)
                                                                                cAAATTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          >  1:3280462/1‑93 (MQ=255)
                                                                                cAAAGTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          <  2:1527786/93‑1 (MQ=255)
                                                                                 aaaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  2:12248/1‑93 (MQ=255)
                                                                                 aaaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  2:1877904/1‑93 (MQ=255)
                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:53640/93‑1 (MQ=255)
                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:3602996/93‑1 (MQ=255)
                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  1:3658048/1‑93 (MQ=255)
                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  2:1720959/1‑93 (MQ=255)
                                                                                  aaTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCCCAAACGCTTAAGCAGCGTTGTGCGGCTGTCGCCTGa        >  1:3111314/1‑93 (MQ=255)
                                                                                   aTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  1:1914282/93‑1 (MQ=255)
                                                                                   aTTGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  1:2036828/93‑1 (MQ=255)
                                                                                    ttGTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      <  1:3445648/93‑1 (MQ=255)
                                                                                      gTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    >  2:4514465/1‑93 (MQ=255)
                                                                                      gTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:2848567/93‑1 (MQ=255)
                                                                                      gTTTC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    >  2:1909346/1‑93 (MQ=255)
                                                                                        ttC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:3272409/93‑1 (MQ=255)
                                                                                        ttC‑GCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:2560435/93‑1 (MQ=255)
                                                                                            |                                                                                         
GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGC‑GGGCAAATTGTTTC‑ACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208242‑2208423
                                                                                         |                                                                                            
CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAG                                                                                          <  SRR4302535.634292/94‑1 (MQ=60)
CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAG                                                                                          <  SRR4302535.2083332/94‑1 (MQ=60)
   CGAAGTGAACAGGAAAACTCACCGGCAGCTACTCGTCGTCTGTTTAGGTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAG                                                                                       <  SRR4302535.2956211/94‑1 (MQ=60)
   CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAG                                                                                       <  SRR4302535.2036879/94‑1 (MQ=60)
   CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAG                                                                                       <  SRR4302535.3289763/94‑1 (MQ=60)
    GAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGC                                                                                      <  SRR4302535.2385333/94‑1 (MQ=60)
      AGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGGAA                                                                                    >  SRR4302535.3898938/1‑94 (MQ=60)
         GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAC                                                                                 <  SRR4302535.3401777/94‑1 (MQ=60)
         GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAAC                                                                                 <  SRR4302535.271785/94‑1 (MQ=60)
           ACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGC                                                                               <  SRR4302535.4065875/94‑1 (MQ=60)
              GGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGT                                                                            <  SRR4302535.3462522/94‑1 (MQ=60)
              GGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGT                                                                            <  SRR4302535.3248224/94‑1 (MQ=60)
              GGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGT                                                                            <  SRR4302535.2825862/94‑1 (MQ=60)
               GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                           <  SRR4302535.1223689/94‑1 (MQ=60)
                AAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGC                                                                          <  SRR4302535.3080807/94‑1 (MQ=60)
                 AAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCCAATTGTTTCGCAAACAGCAAAACGCAGTGCC                                                                         >  SRR4302535.22231/1‑94 (MQ=60)
                 AAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCC                                                                         >  SRR4302535.1283618/1‑94 (MQ=60)
                    CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAT                                                                      <  SRR4302535.2313877/94‑1 (MQ=60)
                    CTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGGTTCGCAAGCAGCAAAAagcaggggccat                                                                      >  SRR4302535.4446177/1‑82 (MQ=60)
                       CCCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGC                                                                   <  SRR4302535.872756/94‑1 (MQ=60)
                       ACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGC                                                                   <  SRR4302535.1535021/94‑1 (MQ=60)
                           GCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGC                                                               <  SRR4302535.650659/94‑1 (MQ=60)
                             AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCCGTGCCGATGGCGAGCTG                                                             >  SRR4302535.712964/1‑94 (MQ=60)
                             AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTG                                                             >  SRR4302535.3535937/1‑94 (MQ=60)
                               CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                           <  SRR4302535.1481731/94‑1 (MQ=60)
                               CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                           <  SRR4302535.3253381/94‑1 (MQ=60)
                                TACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTG                                                          <  SRR4302535.1148256/94‑1 (MQ=60)
                                 ACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGT                                                         >  SRR4302535.3199158/1‑94 (MQ=60)
                                  GGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCTGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTT                                                        <  SRR4302535.2675695/94‑1 (MQ=60)
                                  CGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTT                                                        >  SRR4302535.3708689/1‑94 (MQ=60)
                                    CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      <  SRR4302535.4526221/94‑1 (MQ=60)
                                    CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      >  SRR4302535.2187966/1‑94 (MQ=60)
                                    CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                      <  SRR4302535.313291/94‑1 (MQ=60)
                                     GTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGC                                                     >  SRR4302535.5069/1‑94 (MQ=60)
                                      TCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCT                                                    <  SRR4302535.2581996/94‑1 (MQ=60)
                                       CGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTA                                                   >  SRR4302535.1004111/1‑94 (MQ=60)
                                        GGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGGTGCTGTTAGCTAC                                                  >  SRR4302535.260794/1‑94 (MQ=60)
                                        GGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAC                                                  <  SRR4302535.856197/94‑1 (MQ=60)
                                          CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                                <  SRR4302535.1227749/94‑1 (MQ=60)
                                                AGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGGAGTGCCGATTGCGAGCTGCTGTTAGTTACCCGCAACC                                          >  SRR4302535.3582093/1‑94 (MQ=60)
                                                AGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACC                                          <  SRR4302535.3983943/94‑1 (MQ=60)
                                                 GTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCG                                         <  SRR4302535.4343676/94‑1 (MQ=60)
                                                   TTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCT                                       <  SRR4302535.2775323/94‑1 (MQ=60)
                                                    TTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTT                                      <  SRR4302535.2845680/94‑1 (MQ=60)
                                                        GGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGC                                  >  SRR4302535.2756479/1‑94 (MQ=60)
                                                         GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302535.468388/94‑1 (MQ=60)
                                                         GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 >  SRR4302535.1564901/1‑94 (MQ=60)
                                                         GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                                 <  SRR4302535.2483107/94‑1 (MQ=60)
                                                           TGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGC                               <  SRR4302535.4126421/94‑1 (MQ=60)
                                                           TGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGC                               <  SRR4302535.841327/94‑1 (MQ=60)
                                                             GCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGT                             >  SRR4302535.2385490/1‑94 (MQ=60)
                                                               GCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTG                           <  SRR4302535.641191/94‑1 (MQ=60)
                                                                   TGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCC                       <  SRR4302535.3816763/94‑1 (MQ=60)
                                                                      CGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCT                    <  SRR4302535.2209860/94‑1 (MQ=60)
                                                                        GGCGGGCAAATTGTTTCGCAAGCAGCAAAGCGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGT                  <  SRR4302535.3262884/94‑1 (MQ=60)
                                                                         GCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTC                 <  SRR4302535.2307539/94‑1 (MQ=60)
                                                                         GCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTC                 <  SRR4302535.781819/94‑1 (MQ=60)
                                                                             GCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCT             >  SRR4302535.3303247/1‑94 (MQ=60)
                                                                              CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG            >  SRR4302535.528093/1‑94 (MQ=60)
                                                                               AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGA           >  SRR4302535.3683370/1‑94 (MQ=60)
                                                                               AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCCCAAACGCTTAAGCAGCGTTGTGCGGCTGTCGCCTGA           >  SRR4302535.3132976/1‑94 (MQ=60)
                                                                                AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302535.54034/94‑1 (MQ=60)
                                                                                AATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAT          <  SRR4302535.3627998/94‑1 (MQ=60)
                                                                                 ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         <  SRR4302535.1928077/94‑1 (MQ=60)
                                                                                 ATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATG         <  SRR4302535.2051495/94‑1 (MQ=60)
                                                                                  TTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGC        <  SRR4302535.3469605/94‑1 (MQ=60)
                                                                                    GTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCT      <  SRR4302535.4357679/94‑1 (MQ=60)
                                                                                     TTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG     <  SRR4302535.3313904/94‑1 (MQ=60)
                                                                                      TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    <  SRR4302535.1244633/94‑1 (MQ=60)
                                                                                      TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    <  SRR4302535.2578867/94‑1 (MQ=60)
                                                                                      TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG    <  SRR4302535.3295142/94‑1 (MQ=60)
                                                                                        CGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  <  SRR4302535.2604796/94‑1 (MQ=60)
                                                                                         |                                                                                            
CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCA  >  NC_000913/2208242‑2208423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑