Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,246,351	T→C	S68G (AGT→GGT)	lysP ←	lysine‑specific permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,246,351	0	T	C	99.7%	928.0 / NA	297	S68G (AGT→GGT)	lysP	lysine‑specific permease
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (162/134); total (163/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 299 total aligned reads displayed.

GCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGG  >  NC_000913/2246261‑2246440

   cgAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtca                                                                                      >  2:6505857/1‑93 (MQ=255)

   cgAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtca                                                                                      >  2:5568215/1‑93 (MQ=255)

      aGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatca                                                                                   >  1:5053787/1‑93 (MQ=255)

       gCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAg                                                                                  <  2:816003/93‑1 (MQ=255)

         cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGa                                                                                <  2:7318509/93‑1 (MQ=255)

         cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGa                                                                                >  1:4079590/1‑93 (MQ=255)

          aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGaa                                                                               <  1:2221085/93‑1 (MQ=255)

          aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGaa                                                                               >  2:4589203/1‑93 (MQ=255)

           aaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAg                                                                              >  2:859863/1‑93 (MQ=255)

           aaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAg                                                                              >  1:6278489/1‑93 (MQ=255)

             gCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTa                                                                            >  2:6655309/1‑93 (MQ=255)

             gCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTa                                                                            >  2:4985514/1‑93 (MQ=255)

               cttcttCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTaaa                                                                          >  2:1153605/1‑93 (MQ=255)

                ttcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         <  2:1665979/93‑1 (MQ=255)

                ttcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         <  1:3390248/93‑1 (MQ=255)

                 tcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAcc                                                                        >  2:2654351/1‑93 (MQ=255)

                  cttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCa                                                                       <  2:2927544/93‑1 (MQ=255)

                  cttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCa                                                                       <  1:6663890/93‑1 (MQ=255)

                   ttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAt                                                                      <  1:3507508/93‑1 (MQ=255)

                   ttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAt                                                                      >  1:7377792/1‑93 (MQ=255)

                   ttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAt                                                                      >  1:4141843/1‑93 (MQ=255)

                    tcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATc                                                                     <  1:894826/93‑1 (MQ=255)

                     cAACATAGTTCTGACCGTAAGTGGCAAACGACCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCa                                                                    <  1:7790965/93‑1 (MQ=255)

                      caCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAg                                                                   <  2:5347336/92‑1 (MQ=255)

                      aaCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAg                                                                   <  1:3790711/93‑1 (MQ=255)

                      aaCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAg                                                                   <  2:670714/93‑1 (MQ=255)

                        cATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGc                                                                 <  1:2630847/93‑1 (MQ=255)

                        cATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGc                                                                 >  1:1336689/1‑93 (MQ=255)

                         aTAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGcc                                                                <  1:5665033/93‑1 (MQ=255)

                            gTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAAt                                                             <  1:2438738/93‑1 (MQ=255)

                             ttCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATc                                                            >  2:7766739/1‑93 (MQ=255)

                                tGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGc                                                         >  2:6838898/1‑93 (MQ=255)

                                 gACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCa                                                        <  1:1108610/93‑1 (MQ=255)

                                 gACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCa                                                        <  1:7190230/93‑1 (MQ=255)

                                   ccGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatc                                                      <  2:4794397/93‑2 (MQ=255)

                                   ccGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCata                                                      <  1:652135/93‑1 (MQ=255)

                                   ccGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCata                                                      <  1:2898987/93‑1 (MQ=255)

                                    cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatat                                                     <  1:3956024/93‑1 (MQ=255)

                                    cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatat                                                     <  2:7603864/93‑1 (MQ=255)

                                     gTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAtata                                                    >  2:3581046/1‑93 (MQ=255)

                                     gTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAtata                                                    <  2:7390100/93‑1 (MQ=255)

                                     gTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATAAGCAtata                                                    >  2:2083786/1‑93 (MQ=255)

                                      tAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                   >  2:7191496/1‑93 (MQ=255)

                                      tAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                   <  1:5404651/93‑1 (MQ=255)

                                      tAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                   >  1:2819569/1‑93 (MQ=255)

                                       aaGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACg                                                  <  1:5507456/93‑1 (MQ=255)

                                        aGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACga                                                 >  1:3339303/1‑93 (MQ=255)

                                         gTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgag                                                >  1:927910/1‑93 (MQ=255)

                                         gTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgag                                                >  1:3103102/1‑93 (MQ=255)

                                           ggCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGagag                                              >  2:3264865/1‑93 (MQ=255)

                                           ggCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGagag                                              <  1:859773/93‑1 (MQ=255)

                                            gCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGc                                             <  1:3536469/93‑1 (MQ=255)

                                              aaaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCaa                                           <  2:1440202/93‑1 (MQ=255)

                                              aaaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCaa                                           >  2:317518/1‑93 (MQ=255)

                                               atctaaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAt                                          <  1:493013/89‑1 (MQ=255)

                                               aaCGACCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAt                                          <  2:4311252/93‑1 (MQ=255)

                                                aCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATg                                         >  2:3086236/1‑93 (MQ=255)

                                                 cGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGc                                        <  2:5243175/93‑1 (MQ=255)

                                                  gAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGcc                                       >  1:2967806/1‑93 (MQ=255)

                                                  gAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGcc                                       >  1:4404829/1‑93 (MQ=255)

                                                  gAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGcc                                       >  2:2509169/1‑93 (MQ=255)

                                                   aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGccc                                      >  2:3973894/1‑93 (MQ=255)

                                                   aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGccc                                      >  2:5731259/1‑93 (MQ=255)

                                                   aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGccc                                      <  1:416106/93‑1 (MQ=255)

                                                       ggAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcc                                  >  1:4469596/1‑93 (MQ=255)

                                                       ggAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcc                                  <  1:1187403/93‑1 (MQ=255)

                                                       ggAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcc                                  <  2:4698098/93‑1 (MQ=255)

                                                        gAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGATAGCAATGCccggccc                                 >  2:5545891/1‑93 (MQ=255)

                                                         aaaCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcccg                                >  1:1409534/1‑93 (MQ=255)

                                                          aaCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcccgc                               >  1:1827605/1‑92 (MQ=255)

                                                          aaCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCgg                               >  1:151974/1‑93 (MQ=255)

                                                           aCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGa                              <  2:2897480/93‑1 (MQ=255)

                                                            ccGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                             <  2:2346948/93‑1 (MQ=255)

                                                            ccGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                             >  2:6338535/1‑93 (MQ=255)

                                                            ccGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                             >  2:2726613/1‑93 (MQ=255)

                                                             cGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcc                            >  2:5975852/1‑93 (MQ=255)

                                                              ggCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcct                           <  1:7732523/93‑1 (MQ=255)

                                                               gCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctg                          >  2:6202666/1‑93 (MQ=255)

                                                                 atatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcc                        >  2:2996916/1‑93 (MQ=255)

                                                                  tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       >  1:1009238/1‑93 (MQ=255)

                                                                  tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       >  2:6596042/1‑93 (MQ=255)

                                                                  tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       >  1:6143828/1‑93 (MQ=255)

                                                                  tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       >  2:2959535/1‑93 (MQ=255)

                                                                    tatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTga                     >  1:3287183/1‑93 (MQ=255)

                                                                    tatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTga                     >  2:3323864/1‑93 (MQ=255)

                                                                        caaccAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGaaa                 <  1:7825223/90‑1 (MQ=255)

                                                                         aGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAt                <  2:1779092/93‑1 (MQ=255)

                                                                          gCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc               <  1:1972200/93‑1 (MQ=255)

                                                                            cAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGt             <  1:7646802/93‑1 (MQ=255)

                                                                             aGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGtt            >  1:6075629/1‑93 (MQ=255)

                                                                             aGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGtt            >  2:3982752/1‑93 (MQ=255)

                                                                              gTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTg           >  1:4063431/1‑93 (MQ=255)

                                                                              gTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTg           >  1:4818325/1‑93 (MQ=255)

                                                                               ttCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgc          >  2:4414490/1‑93 (MQ=255)

                                                                                 cACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgcgc        >  2:6986230/1‑93 (MQ=255)

                                                                                 cACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgcgc        <  2:7568209/93‑1 (MQ=255)

                                                                                  aCCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGcc       <  1:42095/93‑1 (MQ=255)

                                                                                     gagaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCaga    >  2:7879967/1‑93 (MQ=255)

                                                                                      agaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCagag   <  2:3858472/93‑1 (MQ=255)

                                                                                       gaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAgg  <  1:3593414/93‑1 (MQ=255)

GCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGG  >  NC_000913/2246261‑2246440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAA  >  NC_000913/2246258‑2246443

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACC                                                                                              >  SRR4302543.5662263/1‑94 (MQ=60)

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACC                                                                                              <  SRR4302543.228018/94‑1 (MQ=60)

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTAACCGAGACC                                                                                              <  SRR4302543.2610474/94‑1 (MQ=60)

 CAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCG                                                                                             >  SRR4302543.1559681/1‑94 (MQ=60)

  AGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGG                                                                                            >  SRR4302543.2046632/1‑94 (MQ=60)

  AGCGCGAAGCCAAAGCCTTCTTAAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGG                                                                                            <  SRR4302543.7106548/94‑1 (MQ=60)

   GCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGGCCGGT                                                                                           <  SRR4302543.72645/94‑1 (MQ=60)

     GCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCA                                                                                         >  SRR4302543.7831329/1‑94 (MQ=60)

        AAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCG                                                                                      >  SRR4302543.5099734/1‑94 (MQ=60)

        AAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCA                                                                                      <  SRR4302543.1889210/94‑1 (MQ=60)

        AAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCA                                                                                      >  SRR4302543.5099735/1‑94 (MQ=60)

         AGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAG                                                                                     >  SRR4302543.418109/1‑94 (MQ=60)

          GCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGG                                                                                    <  SRR4302543.4076282/94‑1 (MQ=60)

           CCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGA                                                                                   >  SRR4302543.4116159/1‑94 (MQ=60)

            CAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCATGAA                                                                                  <  SRR4302543.1170737/94‑1 (MQ=60)

            CAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAA                                                                                  >  SRR4302543.5487393/1‑94 (MQ=60)

             AAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAG                                                                                 <  SRR4302543.2241128/94‑1 (MQ=60)

             AAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAG                                                                                 <  SRR4302543.3351173/94‑1 (MQ=60)

             AAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAG                                                                                 >  SRR4302543.6337671/1‑94 (MQ=60)

              AAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGT                                                                                >  SRR4302543.4944118/1‑94 (MQ=60)

               AGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGGAGCGAGTTCACCGAGACCGGTCATCAGGAAGTA                                                                               >  SRR4302543.1769331/1‑94 (MQ=60)

               AGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTA                                                                               >  SRR4302543.891074/1‑94 (MQ=60)

                GCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAA                                                                              >  SRR4302543.1334750/1‑94 (MQ=60)

                ACCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAA                                                                              <  SRR4302543.2476443/94‑1 (MQ=60)

                  CTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAATCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAC                                                                            <  SRR4302543.2954135/94‑1 (MQ=60)

                  CTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAC                                                                            >  SRR4302543.934848/1‑94 (MQ=60)

                   TTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACC                                                                           <  SRR4302543.3420645/94‑1 (MQ=60)

                     CTTCAACATAGTTCTGACCGTAAGTGGCAAACGCACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCCTCAGGCAGTCAACCAC                                                                         >  SRR4302543.4179248/1‑94 (MQ=60)

                     CTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAT                                                                         >  SRR4302543.7448712/1‑94 (MQ=60)

                     CTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAT                                                                         <  SRR4302543.6733007/94‑1 (MQ=60)

                     CTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAT                                                                         <  SRR4302543.6726807/94‑1 (MQ=60)

                     CTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAT                                                                         >  SRR4302543.4179249/1‑94 (MQ=60)

                      TTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATC                                                                        <  SRR4302543.3538860/94‑1 (MQ=60)

                       TCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCA                                                                       <  SRR4302543.1617692/94‑1 (MQ=60)

                       TCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCA                                                                       <  SRR4302543.902733/94‑1 (MQ=60)

                        CAACATAGTTCTGACCGTAAGTGGCAAACGACCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAG                                                                      <  SRR4302543.7864395/94‑1 (MQ=60)

                        CAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAG                                                                      >  SRR4302543.1506471/1‑94 (MQ=60)

                        CAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAG                                                                      >  SRR4302543.932817/1‑94 (MQ=60)

                         AACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGG                                                                     <  SRR4302543.7962694/94‑1 (MQ=60)

                         AACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGG                                                                     <  SRR4302543.3824108/94‑1 (MQ=60)

                          ACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGC                                                                    >  SRR4302543.1082622/1‑94 (MQ=60)

                          ACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGC                                                                    >  SRR4302543.1348609/1‑94 (MQ=60)

                           CATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCC                                                                   <  SRR4302543.6598641/94‑1 (MQ=60)

                           CATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCC                                                                   <  SRR4302543.2654661/94‑1 (MQ=60)

                            ATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCA                                                                  <  SRR4302543.5716712/94‑1 (MQ=60)

                               GTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATC                                                               <  SRR4302543.2460730/94‑1 (MQ=60)

                                TTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCA                                                              <  SRR4302543.3578882/94‑1 (MQ=60)

                                TTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCA                                                              <  SRR4302543.2774149/94‑1 (MQ=60)

                                TTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCA                                                              >  SRR4302543.7847182/1‑94 (MQ=60)

                                TTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCA                                                              <  SRR4302543.3361056/94‑1 (MQ=60)

                                 TCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAG                                                             <  SRR4302543.2745973/94‑1 (MQ=60)

                                  CTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGC                                                            <  SRR4302543.4201623/94‑1 (MQ=60)

                                   TGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCA                                                           <  SRR4302543.6431101/94‑1 (MQ=60)

                                    GACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAT                                                          <  SRR4302543.6146649/94‑1 (MQ=60)

                                    GACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAT                                                          <  SRR4302543.7259970/94‑1 (MQ=60)

                                    GACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAT                                                          <  SRR4302543.1118615/94‑1 (MQ=60)

                                     ACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATA                                                         >  SRR4302543.3504222/1‑94 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCGCCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                        <  SRR4302543.869163/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCTGGAAGTAAACCATCAGGCCAATCAGCATAT                                                        <  SRR4302543.658106/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                        <  SRR4302543.56026/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                        >  SRR4302543.2586823/1‑94 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                        <  SRR4302543.658107/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                        <  SRR4302543.3573721/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                        <  SRR4302543.2925344/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCAAATCAGCATAT                                                        <  SRR4302543.6611775/94‑1 (MQ=60)

                                       CGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATA                                                       >  SRR4302543.5576095/1‑94 (MQ=60)

                                       CGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATA                                                       <  SRR4302543.4105194/94‑1 (MQ=60)

                                       CGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATA                                                       <  SRR4302543.3991034/94‑1 (MQ=60)

                                        GTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAC                                                      >  SRR4302543.2845373/1‑94 (MQ=60)

                                        GTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAC                                                      <  SRR4302543.776750/94‑1 (MQ=60)

                                         TAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACG                                                     <  SRR4302543.6191481/94‑1 (MQ=60)

                                         TAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACG                                                     <  SRR4302543.5453785/94‑1 (MQ=60)

                                         TAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACG                                                     >  SRR4302543.5142457/1‑94 (MQ=60)

                                          AAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGA                                                    >  SRR4302543.7105250/1‑94 (MQ=60)

                                          AAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGA                                                    >  SRR4302543.3369385/1‑94 (MQ=60)

                                          AAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGA                                                    <  SRR4302543.5557457/94‑1 (MQ=60)

                                          AAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCAACGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGA                                                    >  SRR4302543.3369386/1‑94 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                   >  SRR4302543.3131007/1‑94 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                   >  SRR4302543.2324482/1‑94 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                   >  SRR4302543.936023/1‑94 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                   <  SRR4302543.4107153/94‑1 (MQ=60)

                                              GGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGC                                                <  SRR4302543.867433/94‑1 (MQ=60)

                                              GGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGC                                                <  SRR4302543.7360629/94‑1 (MQ=60)

                                              GGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGC                                                <  SRR4302543.3879267/94‑1 (MQ=60)

                                               GCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCA                                               <  SRR4302543.3567927/94‑1 (MQ=60)

                                                CAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAA                                              >  SRR4302543.7867439/1‑94 (MQ=60)

                                                CAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAA                                              <  SRR4302543.943586/94‑1 (MQ=60)

                                                CAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAA                                              >  SRR4302543.3273687/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                             <  SRR4302543.7434891/94‑1 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                             >  SRR4302543.3029600/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                             >  SRR4302543.4050003/1‑94 (MQ=60)

                                                  atctAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                            <  SRR4302543.497570/90‑1 (MQ=60)

                                                  AACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                            >  SRR4302543.4804347/1‑94 (MQ=60)

                                                   ACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGC                                           <  SRR4302543.5831562/94‑1 (MQ=60)

                                                    CGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCC                                          >  SRR4302543.2994756/1‑94 (MQ=60)

                                                    CGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCC                                          >  SRR4302543.4444766/1‑94 (MQ=60)

                                                    CGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCC                                          >  SRR4302543.2994755/1‑94 (MQ=60)

                                                    CGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCC                                          <  SRR4302543.4433990/94‑1 (MQ=60)

                                                     GAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGCAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCC                                         >  SRR4302543.2185580/1‑94 (MQ=60)

                                                      AACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCC                                        <  SRR4302543.5081756/94‑1 (MQ=60)

                                                      AACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCC                                        <  SRR4302543.419994/94‑1 (MQ=60)

                                                         CGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCC                                     >  SRR4302543.4510126/1‑94 (MQ=60)

                                                         CGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCC                                     >  SRR4302543.6335821/1‑94 (MQ=60)

                                                         CGGAAACCGGCATATATGCAGACAGTTCACCGAGACCGGTCATCAGGAAGTAAACAATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCC                                     >  SRR4302543.4510125/1‑94 (MQ=60)

                                                          GGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCC                                    <  SRR4302543.1198115/94‑1 (MQ=60)

                                                          GGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCC                                    >  SRR4302543.3430579/1‑94 (MQ=60)

                                                          GGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCC                                    <  SRR4302543.1893593/94‑1 (MQ=60)

                                                           GAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCG                                   >  SRR4302543.1422271/1‑94 (MQ=60)

                                                            AAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGG                                  >  SRR4302543.153290/1‑94 (MQ=60)

                                                            AAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGC                                  >  SRR4302543.1844015/1‑94 (MQ=60)

                                                               CCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACC                               >  SRR4302543.6013901/1‑94 (MQ=60)

                                                               CCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACC                               >  SRR4302543.4269791/1‑94 (MQ=60)

                                                                CGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCT                              <  SRR4302543.3924999/94‑1 (MQ=60)

                                                                CGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCT                              <  SRR4302543.7646558/94‑1 (MQ=60)

                                                                CGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCT                              >  SRR4302543.2853211/1‑94 (MQ=60)

                                                                 GGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTG                             <  SRR4302543.7805593/94‑1 (MQ=60)

                                                                   CATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCC                           >  SRR4302543.7747280/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                          >  SRR4302543.1018166/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                          >  SRR4302543.6202018/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                          >  SRR4302543.2339630/1‑94 (MQ=60)

                                                                     TATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTG                         <  SRR4302543.5537154/94‑1 (MQ=60)

                                                                      ATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGA                        >  SRR4302543.4460819/1‑94 (MQ=60)

                                                                      ATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGA                        >  SRR4302543.3316851/1‑94 (MQ=60)

                                                                      ATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGA                        >  SRR4302543.4131217/1‑94 (MQ=60)

                                                                      ATATGCAGCCAGTTCACCGAGACCGGCCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGA                        >  SRR4302543.844945/1‑94 (MQ=60)

                                                                       TATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAG                       >  SRR4302543.1379085/1‑94 (MQ=60)

                                                                        ATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGA                      >  SRR4302543.3183931/1‑94 (MQ=60)

                                                                           CAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAT                   >  SRR4302543.7484606/1‑94 (MQ=60)

                                                                           CAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAT                   >  SRR4302543.1189811/1‑94 (MQ=60)

                                                                           CAACCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAT                   <  SRR4302543.7898927/94‑1 (MQ=60)

                                                                             GCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCG                 <  SRR4302543.3554752/94‑1 (MQ=60)

                                                                             GCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCG                 <  SRR4302543.1989878/94‑1 (MQ=60)

                                                                              CCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGT                >  SRR4302543.1252566/1‑94 (MQ=60)

                                                                              CCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGT                <  SRR4302543.1414030/94‑1 (MQ=60)

                                                                               CAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTT               <  SRR4302543.7719316/94‑1 (MQ=60)

                                                                               CAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTT               >  SRR4302543.6133214/1‑94 (MQ=60)

                                                                               CAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTT               <  SRR4302543.2905542/94‑1 (MQ=60)

                                                                               CAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTT               <  SRR4302543.4533806/94‑1 (MQ=60)

                                                                               CAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCACCGCCCGGACCTGCCTGAGAAATCGTT               >  SRR4302543.1473626/1‑94 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG              >  SRR4302543.5332286/1‑94 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG              >  SRR4302543.4862321/1‑94 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG              >  SRR4302543.4099817/1‑94 (MQ=60)

                                                                                 GTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCGGCCCGGACCTGCCTGAGAAATCGTTGC             >  SRR4302543.3380739/1‑94 (MQ=60)

                                                                                 GTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGC             >  SRR4302543.306528/1‑94 (MQ=60)

                                                                                 GTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGC             >  SRR4302543.6315676/1‑94 (MQ=60)

                                                                                   TCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGC           >  SRR4302543.4184597/1‑94 (MQ=60)

                                                                                   TCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGC           <  SRR4302543.3817261/94‑1 (MQ=60)

                                                                                    CACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCC          >  SRR4302543.4242350/1‑94 (MQ=60)

                                                                                     ACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCA         <  SRR4302543.3564803/94‑1 (MQ=60)

                                                                                     ACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCA         <  SRR4302543.42480/94‑1 (MQ=60)

                                                                                      CCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAG        >  SRR4302543.4948704/1‑94 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG      >  SRR4302543.78857/1‑94 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG      <  SRR4302543.3799908/94‑1 (MQ=60)

                                                                                          GACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGC    <  SRR4302543.3625133/94‑1 (MQ=60)

                                                                                          GACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGC    >  SRR4302543.6902233/1‑94 (MQ=60)

                                                                                           ACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCA   >  SRR4302543.5178456/1‑94 (MQ=60)

                                                                                            CCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAA  >  SRR4302543.5651858/1‑94 (MQ=60)

                                                                                            CCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAA  >  SRR4302543.4018701/1‑94 (MQ=60)

                                                                                            CCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAA  <  SRR4302543.5984915/94‑1 (MQ=60)

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAA  >  NC_000913/2246258‑2246443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`