Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,288,635	G→A	G39D (GGC→GAC)	narP →	nitrate/nitrite response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,288,635	0	G	A	99.6%	920.0 / ‑5.7	255	G39D (GGC→GAC)	narP	nitrate/nitrite response regulator
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (113/141); total (114/141)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 270 total aligned reads displayed.

TGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGGCGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGG  >  NC_000913/2288545‑2288724

 gatgatTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGa                                                                                        <  2:1721043/93‑1 (MQ=255)

 gatgatTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGa                                                                                        <  2:2009647/93‑1 (MQ=255)

  atgatTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGgacgac                                                                                       >  1:2218375/1‑93 (MQ=255)

  atgatTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGgacgac                                                                                       >  1:3273933/1‑93 (MQ=255)

  atgatTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGag                                                                                       >  2:723864/1‑92 (MQ=255)

    gatTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACgg                                                                                     <  2:656968/93‑1 (MQ=255)

     atTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGgc                                                                                    <  1:2886270/93‑1 (MQ=255)

     atTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGGCAGTTACTGGAGGTTGATCCTGGCTCTGAAGTGGCCGCCGAAGCGGACGACGgc                                                                                    <  1:3183240/93‑1 (MQ=255)

        gtgGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGcgcg                                                                                 >  1:1554976/1‑93 (MQ=255)

           gatgatCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAgc                                                                              >  1:857528/1‑93 (MQ=255)

             tgatCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGCAGCGGACGACGGCGCGAgcgc                                                                            >  2:6979577/1‑93 (MQ=255)

                tcatcCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTAt                                                                         <  1:6820902/93‑1 (MQ=255)

                tcatcCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTAt                                                                         <  1:343888/93‑1 (MQ=255)

                 catcCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATc                                                                        <  1:1704147/93‑1 (MQ=255)

                 catcCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATc                                                                        >  2:5448221/1‑93 (MQ=255)

                  ttaaaCTTAGGCGACGCGGTGTTAGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCg                                                                       <  2:5691926/89‑1 (MQ=255)

                   tcCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGa                                                                      <  1:3778674/93‑1 (MQ=255)

                   tcCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGa                                                                      >  1:7775033/1‑93 (MQ=255)

                    ccACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGAt                                                                     >  1:5006671/1‑93 (MQ=255)

                     cACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATc                                                                    >  1:6341838/1‑93 (MQ=255)

                     cACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATc                                                                    <  1:6152007/93‑1 (MQ=255)

                     cACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATc                                                                    >  2:4457232/1‑93 (MQ=255)

                      aCTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCt                                                                   >  2:1827301/1‑93 (MQ=255)

                       cTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTg                                                                  >  2:6806251/1‑93 (MQ=255)

                       cTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTg                                                                  <  1:6652420/93‑1 (MQ=255)

                       cTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTg                                                                  <  1:5242035/93‑1 (MQ=255)

                        ttATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTgg                                                                 >  1:7549930/1‑93 (MQ=255)

                        ttATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTgg                                                                 <  1:6988630/93‑1 (MQ=255)

                        ttATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTgg                                                                 <  2:1468052/93‑1 (MQ=255)

                          aTGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCg                                                               >  2:2894234/1‑93 (MQ=255)

                           tGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGa                                                              >  2:6057565/1‑93 (MQ=255)

                            gCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGaa                                                             <  2:4813023/93‑1 (MQ=255)

                             cGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATGGATCTGGCGAAt                                                            >  1:846161/1‑93 (MQ=255)

                              gACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATa                                                           <  2:3137730/93‑1 (MQ=255)

                              gACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATa                                                           >  2:2144911/1‑93 (MQ=255)

                               aCGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAg                                                          >  1:3852620/1‑93 (MQ=255)

                                  cgGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACt                                                       <  1:5929977/93‑1 (MQ=255)

                                   ggTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTg                                                      >  1:3500300/1‑93 (MQ=255)

                                   ggTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTg                                                      >  2:3252831/1‑93 (MQ=255)

                                    gtgtTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTgg                                                     >  2:5542932/1‑93 (MQ=255)

                                     tgtTCGTCAGTTACTGGAGCTTGATCCTTGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGa                                                    <  1:2318285/93‑1 (MQ=255)

                                     tgtTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGa                                                    <  2:652529/93‑1 (MQ=255)

                                      tttCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGat                                                   <  2:3114490/92‑1 (MQ=255)

                                      gtTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGat                                                   >  2:4093440/1‑93 (MQ=255)

                                       ttCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGata                                                  <  2:6492723/93‑1 (MQ=255)

                                        tCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGatat                                                 >  2:792296/1‑93 (MQ=255)

                                         cGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATc                                                <  1:6666895/93‑1 (MQ=255)

                                           tCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGa                                              <  2:4205605/93‑1 (MQ=255)

                                           tCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGa                                              <  1:6540493/93‑1 (MQ=255)

                                           tCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGa                                              >  2:1224546/1‑93 (MQ=255)

                                           tCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGAGCTATCGATCTGGCGAATAGACTGGATATCGa                                              <  2:6868004/93‑1 (MQ=255)

                                            cAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGAc                                             <  1:7270704/93‑1 (MQ=255)

                                              gTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGt                                           <  2:3036307/93‑1 (MQ=255)

                                              gTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGt                                           <  2:6105287/93‑1 (MQ=255)

                                              gTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGt                                           >  2:4956887/1‑93 (MQ=255)

                                               ttACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTg                                          <  1:138896/93‑1 (MQ=255)

                                                tACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGa                                         <  1:5839358/93‑1 (MQ=255)

                                                tACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGa                                         <  2:250245/93‑1 (MQ=255)

                                                 aCTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGAt                                        <  2:4703518/93‑1 (MQ=255)

                                                  cTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATc                                       >  2:7531302/1‑93 (MQ=255)

                                                   tGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCt                                      <  2:717742/93‑1 (MQ=255)

                                                   tGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCt                                      <  1:6016914/93‑1 (MQ=255)

                                                    ggAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCtt                                     >  2:91772/1‑93 (MQ=255)

                                                       gCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCt                                  >  2:5908064/1‑93 (MQ=255)

                                                       gCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCt                                  >  2:2968862/1‑93 (MQ=255)

                                                       gCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCt                                  <  1:1576992/93‑1 (MQ=255)

                                                        cTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTg                                 <  1:606493/93‑1 (MQ=255)

                                                         ttGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTgg                                >  2:3065425/1‑93 (MQ=255)

                                                          tGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGa                               <  2:6299971/93‑1 (MQ=255)

                                                          tGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGa                               >  1:4167441/1‑93 (MQ=255)

                                                             tCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTCTCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGAtct                            >  1:5373122/1‑93 (MQ=255)

                                                             tCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGAtct                            >  1:4489993/1‑93 (MQ=255)

                                                                 ggCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAat                        <  1:1197502/93‑1 (MQ=255)

                                                                 ggCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAat                        <  2:566558/93‑1 (MQ=255)

                                                                  gCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAata                       <  2:3805863/93‑1 (MQ=255)

                                                                   ctctGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAatat                      <  1:7102469/93‑1 (MQ=255)

                                                                    tctGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATg                     >  2:1882197/1‑93 (MQ=255)

                                                                     ctGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGa                    <  1:2593649/93‑1 (MQ=255)

                                                                     ctGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGa                    <  2:5992538/93‑1 (MQ=255)

                                                                      tGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGaa                   >  1:1344489/1‑93 (MQ=255)

                                                                      tGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGaa                   >  1:2944681/1‑93 (MQ=255)

                                                                       gAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGaaa                  <  1:1018085/93‑1 (MQ=255)

                                                                       gAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGaaa                  <  1:5571978/93‑1 (MQ=255)

                                                                       gAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGaaa                  >  2:4266147/1‑93 (MQ=255)

                                                                       gAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCCGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGaaa                  >  2:6781339/1‑93 (MQ=255)

                                                                          gTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGt               >  1:2014047/1‑93 (MQ=255)

                                                                           tGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTa              <  2:4499960/93‑1 (MQ=255)

                                                                            ggTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTAt             <  1:1045742/93‑1 (MQ=255)

                                                                            ggTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTAt             <  1:6886853/93‑1 (MQ=255)

                                                                            ggTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTAt             <  1:2846733/93‑1 (MQ=255)

                                                                              tCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGa           >  1:3542999/1‑93 (MQ=255)

                                                                              tCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGa           <  2:4363094/93‑1 (MQ=255)

                                                                               cGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAg          <  1:4324848/93‑1 (MQ=255)

                                                                                gCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGt         <  1:3703408/93‑1 (MQ=255)

                                                                                gCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGt         <  2:1029656/93‑1 (MQ=255)

                                                                                 ccGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTg        <  1:3639955/93‑1 (MQ=255)

                                                                                   gAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGc      <  1:7462397/93‑1 (MQ=255)

                                                                                     aGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCt    <  2:7296299/93‑1 (MQ=255)

                                                                                      gCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTg   <  1:2425468/93‑1 (MQ=255)

                                                                                      gCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTg   >  1:320514/1‑93 (MQ=255)

TGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGGCGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGG  >  NC_000913/2288545‑2288724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGGCGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGGAT  >  NC_000913/2288543‑2288726

GGTGATGATTGTGGATTATCATCCCATTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGAC                                                                                            <  SRR4302543.365447/94‑1 (MQ=60)

  TGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGA                                                                                          >  SRR4302543.3511599/1‑94 (MQ=60)

   GATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGAC                                                                                         >  SRR4302543.3303507/1‑94 (MQ=60)

   GATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGAC                                                                                         >  SRR4302543.2238386/1‑94 (MQ=60)

    ATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACG                                                                                        <  SRR4302543.3833586/94‑1 (MQ=60)

       ATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCG                                                                                     <  SRR4302543.3211949/94‑1 (MQ=60)

       ATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCG                                                                                     <  SRR4302543.2912534/94‑1 (MQ=60)

       ATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGGCAGTTACTGGAGGTTGATCCTGGCTCTGAAGTGGCCGCCGAAGCGGACGACGGCG                                                                                     <  SRR4302543.3211948/94‑1 (MQ=60)

        TTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGC                                                                                    <  SRR4302543.5573791/94‑1 (MQ=60)

        TTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGC                                                                                    >  SRR4302543.1467943/1‑94 (MQ=60)

         TGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCG                                                                                   >  SRR4302543.1568873/1‑94 (MQ=60)

            GGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGC                                                                                >  SRR4302543.865165/1‑94 (MQ=60)

            GGATGATCATCCACTTATGCGACGCGGGGTTCGTCAGTTACTGGAGCTTGATCCGGGCTCTGAAGTGGTCGCCGAAGTGGACGACGGCGCGAGC                                                                                >  SRR4302543.865166/1‑94 (MQ=60)

             GATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCTCGCGCG                                                                               >  SRR4302543.5839728/1‑94 (MQ=60)

             GATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCG                                                                               <  SRR4302543.2815990/94‑1 (MQ=60)

             GATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCG                                                                               <  SRR4302543.3332276/94‑1 (MQ=60)

                 ATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTAT                                                                           >  SRR4302543.4848272/1‑94 (MQ=60)

                  TCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATC                                                                          <  SRR4302543.6885291/94‑1 (MQ=60)

                  TCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATC                                                                          <  SRR4302543.7105258/94‑1 (MQ=60)

                  TCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATC                                                                          <  SRR4302543.347068/94‑1 (MQ=60)

                   CATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCG                                                                         <  SRR4302543.1719411/94‑1 (MQ=60)

                   CATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCG                                                                         >  SRR4302543.6605299/1‑94 (MQ=60)

                    ATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGA                                                                        >  SRR4302543.7848362/1‑94 (MQ=60)

                     TCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGAT                                                                       >  SRR4302543.7934718/1‑94 (MQ=60)

                     TCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGAT                                                                       <  SRR4302543.3811943/94‑1 (MQ=60)

                     TCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGAT                                                                       >  SRR4302543.5052240/1‑94 (MQ=60)

                      CCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATC                                                                      <  SRR4302543.7560814/94‑1 (MQ=60)

                      CCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATC                                                                      >  SRR4302543.7601518/1‑94 (MQ=60)

                      CCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATC                                                                      >  SRR4302543.6401470/1‑94 (MQ=60)

                       CACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCT                                                                     <  SRR4302543.6210250/94‑1 (MQ=60)

                        ACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTG                                                                    >  SRR4302543.3021886/1‑94 (MQ=60)

                        ACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTG                                                                    <  SRR4302543.426067/94‑1 (MQ=60)

                         tttcTGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGGCGCCGAAGCGGCCGACGGCGCGAGCGCTATCGATCTGG                                                                   <  SRR4302543.5289283/90‑1 (MQ=60)

                         CTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGG                                                                   <  SRR4302543.5289282/94‑1 (MQ=60)

                         CTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGG                                                                   <  SRR4302543.6715222/94‑1 (MQ=60)

                         CTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGG                                                                   >  SRR4302543.7621846/1‑94 (MQ=60)

                         CTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGG                                                                   >  SRR4302543.1849560/1‑94 (MQ=60)

                         CTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGG                                                                   <  SRR4302543.1601504/94‑1 (MQ=60)

                         CTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCACTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGG                                                                   <  SRR4302543.2241920/94‑1 (MQ=60)

                          TTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGC                                                                  <  SRR4302543.7054400/94‑1 (MQ=60)

                            ATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGA                                                                <  SRR4302543.1043745/94‑1 (MQ=60)

                            ATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGA                                                                >  SRR4302543.6007262/1‑94 (MQ=60)

                              GCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATGGATCTGGCGAAT                                                              >  SRR4302543.853663/1‑94 (MQ=60)

                              GCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAAT                                                              <  SRR4302543.1103447/94‑1 (MQ=60)

                              GCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAAT                                                              <  SRR4302543.5061859/94‑1 (MQ=60)

                              GCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAAT                                                              >  SRR4302543.853664/1‑94 (MQ=60)

                               CGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATA                                                             <  SRR4302543.7205781/94‑1 (MQ=60)

                               CGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATA                                                             >  SRR4302543.2966823/1‑94 (MQ=60)

                               CGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATA                                                             >  SRR4302543.1578393/1‑94 (MQ=60)

                                GACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAG                                                            >  SRR4302543.4378893/1‑94 (MQ=60)

                                GACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAG                                                            >  SRR4302543.3886663/1‑94 (MQ=60)

                                 ACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGA                                                           >  SRR4302543.7717325/1‑94 (MQ=60)

                                  CGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGAC                                                          >  SRR4302543.3264020/1‑94 (MQ=60)

                                   GCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACT                                                         <  SRR4302543.3725150/94‑1 (MQ=60)

                                    CGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGGCGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTG                                                        >  SRR4302543.3810628/1‑94 (MQ=60)

                                    CGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTG                                                        <  SRR4302543.3029692/94‑1 (MQ=60)

                                    CGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTG                                                        <  SRR4302543.5984194/94‑1 (MQ=60)

                                    CGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTG                                                        >  SRR4302543.3531603/1‑94 (MQ=60)

                                     GGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGG                                                       <  SRR4302543.4592406/94‑1 (MQ=60)

                                     GGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGG                                                       <  SRR4302543.6233751/94‑1 (MQ=60)

                                     GGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGG                                                       <  SRR4302543.6233752/94‑1 (MQ=60)

                                     GGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGG                                                       >  SRR4302543.5272023/1‑94 (MQ=60)

                                     GGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGG                                                       <  SRR4302543.7199547/94‑1 (MQ=60)

                                     GGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGG                                                       <  SRR4302543.1083756/94‑1 (MQ=60)

                                       TGTTCGTCAGTTACTGGAGCTTGATCCTTGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGAT                                                     <  SRR4302543.2339361/94‑1 (MQ=60)

                                       TGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGAT                                                     <  SRR4302543.766282/94‑1 (MQ=60)

                                          TCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATC                                                  <  SRR4302543.6909544/94‑1 (MQ=60)

                                           CGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCG                                                 <  SRR4302543.6729834/94‑1 (MQ=60)

                                            GTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGA                                                >  SRR4302543.4064047/1‑94 (MQ=60)

                                             TTAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGAC                                               <  SRR4302543.2465830/94‑1 (MQ=60)

                                             TCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGAC                                               <  SRR4302543.6602057/94‑1 (MQ=60)

                                              CAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACG                                              >  SRR4302543.2891830/1‑94 (MQ=60)

                                              CAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACG                                              <  SRR4302543.7439169/94‑1 (MQ=60)

                                              CAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACG                                              <  SRR4302543.7340942/94‑1 (MQ=60)

                                              CAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACG                                              <  SRR4302543.3626113/94‑1 (MQ=60)

                                               AGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGT                                             >  SRR4302543.6515643/1‑94 (MQ=60)

                                                GTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTG                                            >  SRR4302543.6207191/1‑94 (MQ=60)

                                                GTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTG                                            <  SRR4302543.2397943/94‑1 (MQ=60)

                                                 TTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGA                                           >  SRR4302543.1287947/1‑94 (MQ=60)

                                                 TTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGA                                           <  SRR4302543.140117/94‑1 (MQ=60)

                                                  TACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGAT                                          <  SRR4302543.5892479/94‑1 (MQ=60)

                                                   ACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATC                                         <  SRR4302543.2461060/94‑1 (MQ=60)

                                                   ACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATC                                         >  SRR4302543.1575527/1‑94 (MQ=60)

                                                    CTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCT                                        <  SRR4302543.3545110/94‑1 (MQ=60)

                                                     TGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTatcctt                                       >  SRR4302543.274053/1‑88 (MQ=60)

                                                     TGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTT                                       >  SRR4302543.5645165/1‑94 (MQ=60)

                                                     TGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTT                                       <  SRR4302543.692773/94‑1 (MQ=60)

                                                     TGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTT                                       <  SRR4302543.6072587/94‑1 (MQ=60)

                                                      GGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTG                                      <  SRR4302543.1530410/94‑1 (MQ=60)

                                                       GAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGC                                     >  SRR4302543.908033/1‑94 (MQ=60)

                                                        AGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCT                                    >  SRR4302543.1390894/1‑94 (MQ=60)

                                                         GCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTG                                   <  SRR4302543.1591067/94‑1 (MQ=60)

                                                         GCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTG                                   >  SRR4302543.21336/1‑94 (MQ=60)

                                                          CTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGG                                  <  SRR4302543.612035/94‑1 (MQ=60)

                                                           TTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGA                                 >  SRR4302543.4205180/1‑94 (MQ=60)

                                                             GATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATC                               <  SRR4302543.6724356/94‑1 (MQ=60)

                                                              ATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTCTCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCT                              >  SRR4302543.5421788/1‑94 (MQ=60)

                                                              ATCCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCT                              >  SRR4302543.4530786/1‑94 (MQ=60)

                                                                CCTGGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCA                            <  SRR4302543.2239726/94‑1 (MQ=60)

                                                                   GGCTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATA                         <  SRR4302543.1208285/94‑1 (MQ=60)

                                                                     CTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATG                       <  SRR4302543.7171455/94‑1 (MQ=60)

                                                                     CTCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATG                       >  SRR4302543.6626910/1‑94 (MQ=60)

                                                                     CTCTGAAGTGGGCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATG                       <  SRR4302543.7171454/94‑1 (MQ=60)

                                                                      TCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGA                      >  SRR4302543.1971140/1‑94 (MQ=60)

                                                                      TCTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGA                      <  SRR4302543.7977695/94‑1 (MQ=60)

                                                                       CTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAA                     <  SRR4302543.2617212/94‑1 (MQ=60)

                                                                       CTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAA                     >  SRR4302543.1356501/1‑94 (MQ=60)

                                                                       CTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAA                     >  SRR4302543.2971420/1‑94 (MQ=60)

                                                                       CTGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAA                     >  SRR4302543.682634/1‑94 (MQ=60)

                                                                        TGAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAA                    <  SRR4302543.2970397/94‑1 (MQ=60)

                                                                         GAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAG                   <  SRR4302543.1027090/94‑1 (MQ=60)

                                                                         GAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAG                   <  SRR4302543.7051636/94‑1 (MQ=60)

                                                                         GAAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAG                   <  SRR4302543.5622684/94‑1 (MQ=60)

                                                                          AAGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGG                  <  SRR4302543.44368/94‑1 (MQ=60)

                                                                           AGTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGT                 >  SRR4302543.2032148/1‑94 (MQ=60)

                                                                            GTGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTA                >  SRR4302543.3351767/1‑94 (MQ=60)

                                                                             TGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTAT               <  SRR4302543.6076369/94‑1 (MQ=60)

                                                                             TGGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTAT               <  SRR4302543.385196/94‑1 (MQ=60)

                                                                              GGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATG              <  SRR4302543.1055097/94‑1 (MQ=60)

                                                                              GGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATG              <  SRR4302543.5320595/94‑1 (MQ=60)

                                                                              GGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATG              <  SRR4302543.6951626/94‑1 (MQ=60)

                                                                              GGTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATG              <  SRR4302543.2872725/94‑1 (MQ=60)

                                                                               GTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGA             >  SRR4302543.3574477/1‑94 (MQ=60)

                                                                               GTCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGA             >  SRR4302543.1309296/1‑94 (MQ=60)

                                                                                TCGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAG            <  SRR4302543.3633435/94‑1 (MQ=60)

                                                                                GCGCCGACGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAG            <  SRR4302543.2840499/94‑1 (MQ=60)

                                                                                 CGCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGT           <  SRR4302543.4364112/94‑1 (MQ=60)

                                                                                  GCCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTG          <  SRR4302543.3735965/94‑1 (MQ=60)

                                                                                  GACGAAGAGGACGACGGCGCTAGCGCTATCGATGTGTCCAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTG          <  SRR4302543.656190/94‑1 (MQ=60)

                                                                                   CCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGG         >  SRR4302543.3481991/1‑94 (MQ=60)

                                                                                   CCGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGG         <  SRR4302543.3671984/94‑1 (MQ=60)

                                                                                    CGAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGC        >  SRR4302543.5912659/1‑94 (MQ=60)

                                                                                     GAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCC       >  SRR4302543.3705838/1‑94 (MQ=60)

                                                                                     GAAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCC       <  SRR4302543.7533875/94‑1 (MQ=60)

                                                                                      AAGCGGACGACGGCGCGAGCGCTATCGATCTGGTGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCT      <  SRR4302543.3954024/94‑1 (MQ=60)

                                                                                      AAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCT      >  SRR4302543.5479204/1‑94 (MQ=60)

                                                                                      AAGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCT      <  SRR4302543.3278924/94‑1 (MQ=60)

                                                                                       AGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTG     >  SRR4302543.6022758/1‑94 (MQ=60)

                                                                                       AGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTG     <  SRR4302543.6831020/94‑1 (MQ=60)

                                                                                       AGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTG     >  SRR4302543.323413/1‑94 (MQ=60)

                                                                                       AGCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGGGGCCTG     >  SRR4302543.6089815/1‑94 (MQ=60)

                                                                                        GCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGG    >  SRR4302543.2165719/1‑94 (MQ=60)

                                                                                        GCGGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGG    <  SRR4302543.2447360/94‑1 (MQ=60)

                                                                                          GGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGGAT  >  SRR4302543.7856944/1‑94 (MQ=60)

                                                                                          GGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGGAT  <  SRR4302543.4441760/94‑1 (MQ=60)

                                                                                          GGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGGAT  <  SRR4302543.3055758/94‑1 (MQ=60)

                                                                                          GGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGGAT  <  SRR4302543.658769/94‑1 (MQ=60)

                                                                                          GGACGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGGAT  >  SRR4302543.3766754/1‑94 (MQ=60)

GGTGATGATTGTGGATGATCATCCACTTATGCGACGCGGTGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTGAAGTGGTCGCCGAAGCGGGCGACGGCGCGAGCGCTATCGATCTGGCGAATAGACTGGATATCGACGTGATCTTGCTGGATCTCAATATGAAAGGTATGAGTGGCCTGGAT  >  NC_000913/2288543‑2288726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`