Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,297,335 A→G G88G (GGT→GGC)  napH ← ferredoxin‑type protein: electron transfer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,297,3350AG99.5% 648.2 / ‑4.9 209G88G (GGT→GGC) napHferredoxin‑type protein: electron transfer
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (98/110);  total (99/110)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 232 total aligned reads displayed.

CGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAA  >  NC_000913/2297245‑2297424
                                                                                          |                                                                                         
cGCTAAGTCGGTATTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCCCGGTGATATTcaccgcgcc                                                                                         <  1:7048596/93‑1 (MQ=255)
cGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATcaccgcgcc                                                                                         >  2:4350762/1‑93 (MQ=255)
 gCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATcaccgcgccg                                                                                        <  2:6458915/93‑1 (MQ=255)
  cTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCgg                                                                                       <  2:3624588/93‑1 (MQ=255)
   tAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGt                                                                                      >  1:648751/1‑93 (MQ=255)
      gTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCaa                                                                                   >  2:5182426/1‑93 (MQ=255)
      gTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCaa                                                                                   <  2:1682417/93‑1 (MQ=255)
       tCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAAc                                                                                  <  2:1540042/93‑1 (MQ=255)
          gTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGcc                                                                               >  1:68659/1‑93 (MQ=255)
            aaTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAc                                                                             <  1:2218004/93‑1 (MQ=255)
            aaTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAc                                                                             <  1:1116271/93‑1 (MQ=255)
            aaTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAc                                                                             <  1:598768/93‑1 (MQ=255)
            aaTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAc                                                                             >  2:3079884/1‑93 (MQ=255)
              tCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTCGCGCCCGGTGATAATCACCGCGCCCGCCAACGCCCCCg                                                                           >  2:4993328/1‑93 (MQ=255)
              tCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCg                                                                           <  1:5832845/93‑1 (MQ=255)
              tCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCg                                                                           >  1:511406/1‑93 (MQ=255)
               cGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGt                                                                          <  1:4291472/93‑1 (MQ=255)
                 ggTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTgg                                                                        <  2:505721/93‑1 (MQ=255)
                 ggTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTgg                                                                        <  1:141104/93‑1 (MQ=255)
                  gTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggc                                                                       <  2:4444109/93‑1 (MQ=255)
                   ttCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggca                                                                      <  1:7812406/93‑1 (MQ=255)
                   ttCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggca                                                                      >  1:5312621/1‑93 (MQ=255)
                        cGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGgcaggcag                                                                 >  1:4807241/1‑93 (MQ=255)
                         gggCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGa                                                                <  2:7847134/93‑1 (MQ=255)
                          ggCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAt                                                               <  2:7181356/93‑1 (MQ=255)
                          ggCAGACCCAACTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAt                                                               <  2:2448441/93‑1 (MQ=255)
                           gCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATg                                                              >  2:5965594/1‑93 (MQ=255)
                              gACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGAcc                                                           <  1:1395779/93‑1 (MQ=255)
                              gACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGAcc                                                           >  1:4342776/1‑93 (MQ=255)
                                cccAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCAc                                                         >  1:4230818/1‑93 (MQ=255)
                                cccAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCAc                                                         >  2:1988164/1‑93 (MQ=255)
                                cccAGCCGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCCCCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCAc                                                         <  2:1841180/93‑1 (MQ=255)
                                 ccAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACt                                                        <  1:6611620/93‑1 (MQ=255)
                                  cAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTg                                                       >  1:6917126/1‑93 (MQ=255)
                                   aGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTgg                                                      <  1:1755985/93‑1 (MQ=255)
                                     cTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCa                                                    <  1:3487577/93‑1 (MQ=255)
                                      tGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCaa                                                   <  2:1905680/93‑1 (MQ=255)
                                       gCAAAATAATCCCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAg                                                  <  2:4095278/93‑1 (MQ=255)
                                         aaaaTAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGAc                                                >  1:2913149/1‑93 (MQ=255)
                                         aaaaTAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGAc                                                >  2:3540144/1‑93 (MQ=255)
                                         aaaaTAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGAc                                                >  2:5678296/1‑93 (MQ=255)
                                           aataatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACtt                                              >  2:3763024/1‑93 (MQ=255)
                                           aataatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACtt                                              >  2:1006212/1‑93 (MQ=255)
                                           aataatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACtt                                              <  1:6619891/93‑1 (MQ=255)
                                           aataatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACtt                                              <  1:5743250/93‑1 (MQ=255)
                                             tgatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTg                                            >  1:7761408/3‑93 (MQ=255)
                                              aatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGc                                           >  1:5970116/1‑93 (MQ=255)
                                               atCGCTTTCCCGCCAGGGCGTATAGCACGGTGATAATTACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTTGCAAGACTTTGCa                                          >  2:7257109/1‑93 (MQ=255)
                                               atCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCa                                          <  2:4758917/93‑1 (MQ=255)
                                               atCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCa                                          >  1:2520694/1‑93 (MQ=255)
                                                tCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAg                                         >  1:210942/1‑93 (MQ=255)
                                                 cGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGc                                        <  2:6581198/93‑1 (MQ=255)
                                                 cGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGc                                        <  1:245078/93‑1 (MQ=255)
                                                  gCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCg                                       >  2:7721602/1‑93 (MQ=255)
                                                    tttCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCACGACTTTGCAGCggc                                     >  2:1370517/1‑91 (MQ=255)
                                                    tttCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtc                                     <  2:6784906/93‑1 (MQ=255)
                                                     ttCCCGCTAGGTCGTAGAGCACGGTAATAATCCCCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGCCAAGGCTTTGCAGCGtca                                    <  2:6968585/93‑1 (MQ=255)
                                                     tacccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtca                                    <  1:7899835/91‑1 (MQ=255)
                                                      tCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtcat                                   <  1:2691232/93‑1 (MQ=255)
                                                       cccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGTAGCGtcatc                                  <  1:1892223/93‑1 (MQ=255)
                                                       cccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtcatc                                  <  1:6346552/93‑1 (MQ=255)
                                                       cccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtcatc                                  >  1:5903795/1‑93 (MQ=255)
                                                          gCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGc                               >  2:4216488/1‑93 (MQ=255)
                                                           ccAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCg                              <  2:5343155/93‑1 (MQ=255)
                                                             aGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGa                            <  2:7650090/93‑1 (MQ=255)
                                                              gggCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGac                           >  2:6379823/1‑92 (MQ=255)
                                                              gggCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAt                           <  1:4459947/93‑1 (MQ=255)
                                                              gggCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAt                           <  1:5127910/93‑1 (MQ=255)
                                                               gtcGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATc                          <  1:3040505/91‑1 (MQ=255)
                                                               ggCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATc                          <  2:2412257/93‑1 (MQ=255)
                                                                gCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCg                         <  2:6224350/93‑1 (MQ=255)
                                                                 cGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCgg                        >  2:7347518/1‑93 (MQ=255)
                                                                  gTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGCTGCCCACTGGCAAGACTTTGCAGCTTCATCAGCGGATCGGt                       <  2:4051237/93‑1 (MQ=255)
                                                                  gTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGt                       <  2:1496829/93‑1 (MQ=255)
                                                                   tAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTc                      >  2:1261380/1‑93 (MQ=255)
                                                                   tAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTc                      <  1:302007/93‑1 (MQ=255)
                                                                     gagCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAg                    >  2:5523669/1‑93 (MQ=255)
                                                                      agCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGc                   <  2:3904823/93‑1 (MQ=255)
                                                                      agCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGc                   <  1:4654196/93‑1 (MQ=255)
                                                                      agCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGc                   <  2:5711460/93‑1 (MQ=255)
                                                                        cACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCgg                 <  2:5599711/93‑1 (MQ=255)
                                                                          cGGGGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAGGACTTTGCAGCGTCATCAGCGTATCTGTCAGCGGaa               <  2:1053203/93‑1 (MQ=255)
                                                                           ggTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGCAc              >  2:3502599/1‑93 (MQ=255)
                                                                           ggTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAAc              <  2:7772130/93‑1 (MQ=255)
                                                                           ggTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAAc              >  2:2983603/1‑93 (MQ=255)
                                                                           ggTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAAc              >  1:3387051/1‑93 (MQ=255)
                                                                           ggTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAAc              <  2:4591520/93‑1 (MQ=255)
                                                                             tGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACgg            <  2:5772612/93‑1 (MQ=255)
                                                                             tGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACgg            <  1:3807344/93‑1 (MQ=255)
                                                                             ggATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACgg            <  1:1839748/92‑1 (MQ=255)
                                                                              gATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACggg           >  1:7294806/1‑92 (MQ=255)
                                                                              gATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgt           <  1:1293108/93‑1 (MQ=255)
                                                                              gATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgt           <  2:1321955/93‑1 (MQ=255)
                                                                               aTAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgtg          >  2:873112/1‑93 (MQ=255)
                                                                                tAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAACGGAACGgtgt         >  1:2184001/1‑93 (MQ=255)
                                                                                   tCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGa      >  1:5521477/1‑93 (MQ=255)
                                                                                     cccgcgccgGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAAt    <  1:5386300/92‑1 (MQ=255)
                                                                                      ccgcgccgGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATa   <  1:697374/93‑1 (MQ=255)
                                                                                       cgcgccgGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATaa  <  2:1209035/93‑1 (MQ=255)
                                                                                       cgcgccgGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATaa  >  1:6202486/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAA  >  NC_000913/2297245‑2297424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAGG  >  NC_000913/2297242‑2297428
                                                                                             |                                                                                             
GTTCGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCG                                                                                               >  SRR4302543.1807619/1‑94 (MQ=60)
 TTCGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGC                                                                                              >  SRR4302543.4223376/1‑94 (MQ=60)
  TCGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCC                                                                                             <  SRR4302543.2643546/94‑1 (MQ=60)
   CGCTAAGTCGGTATTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCCCGGTGATATTCACCGCGCCG                                                                                            <  SRR4302543.7116975/94‑1 (MQ=60)
     CTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGT                                                                                          >  SRR4302543.654686/1‑94 (MQ=60)
     CTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGC                                                                                          >  SRR4302543.5997208/1‑94 (MQ=60)
      TAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTC                                                                                         >  SRR4302543.2543466/1‑94 (MQ=60)
          TCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACG                                                                                     <  SRR4302543.5171947/94‑1 (MQ=60)
          TCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACG                                                                                     <  SRR4302543.6704918/94‑1 (MQ=60)
            GGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCC                                                                                   >  SRR4302543.69281/1‑94 (MQ=60)
               AATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACC                                                                                <  SRR4302543.604254/94‑1 (MQ=60)
               AATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACC                                                                                <  SRR4302543.1126375/94‑1 (MQ=60)
               AATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACC                                                                                <  SRR4302543.2238011/94‑1 (MQ=60)
                ATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCG                                                                               >  SRR4302543.5557888/1‑94 (MQ=60)
                ATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCG                                                                               >  SRR4302543.516160/1‑94 (MQ=60)
                 TCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGT                                                                              <  SRR4302543.5885896/94‑1 (MQ=60)
                  CGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTG                                                                             <  SRR4302543.4330492/94‑1 (MQ=60)
                   GGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGG                                                                            >  SRR4302543.4183027/1‑94 (MQ=60)
                    GGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGC                                                                           <  SRR4302543.142339/94‑1 (MQ=60)
                     GTTCAGCGGGCAGACCCAGCTGCAAAATAATCTCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCA                                                                          <  SRR4302543.1428169/94‑1 (MQ=60)
                     GTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCA                                                                          >  SRR4302543.5360613/1‑94 (MQ=60)
                      TTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGGGTAGAGCACGGTGATAATCACCGCACCGGTAAACCCCACCGTGACAG                                                                         >  SRR4302543.5319093/1‑94 (MQ=60)
                      TTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAG                                                                         <  SRR4302543.7886035/94‑1 (MQ=60)
                       TCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGG                                                                        <  SRR4302543.7854141/94‑1 (MQ=60)
                         AGCGGGCAGACCCAGCTGCAAAATAATCTCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCA                                                                      <  SRR4302543.2435997/94‑1 (MQ=60)
                         AGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCA                                                                      <  SRR4302543.4949268/94‑1 (MQ=60)
                          GCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAG                                                                     >  SRR4302543.4851146/1‑94 (MQ=60)
                          GCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAG                                                                     >  SRR4302543.7331715/1‑94 (MQ=60)
                            cggccgaccccggTGCAAAATAATCCCTTTCCCGCCCGGGCCTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAT                                                                   <  SRR4302543.1352725/81‑1 (MQ=60)
                            GGGCAGACCCAGCTGCAAAATAATCGCTTGCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAT                                                                   <  SRR4302543.7571773/94‑1 (MQ=60)
                             GGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATG                                                                  >  SRR4302543.1159919/1‑94 (MQ=60)
                              GCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGA                                                                 >  SRR4302543.6653513/1‑94 (MQ=60)
                               CAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGAC                                                                <  SRR4302543.2793883/94‑1 (MQ=60)
                                AGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACC                                                               >  SRR4302543.4382163/1‑94 (MQ=60)
                                 GACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCA                                                              <  SRR4302543.1408360/94‑1 (MQ=60)
                                 GACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCA                                                              >  SRR4302543.1818551/1‑94 (MQ=60)
                                  ACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCAC                                                             >  SRR4302543.4269194/1‑94 (MQ=60)
                                   CCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACT                                                            >  SRR4302543.3086215/1‑94 (MQ=60)
                                    CCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTG                                                           >  SRR4302543.6982153/1‑94 (MQ=60)
                                    CCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTG                                                           <  SRR4302543.6673942/94‑1 (MQ=60)
                                     CAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGG                                                          >  SRR4302543.3065803/1‑94 (MQ=60)
                                     CAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGG                                                          >  SRR4302543.6084119/1‑94 (MQ=60)
                                     CAGCTGCAAAATAATCGATTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGG                                                          <  SRR4302543.5420730/94‑1 (MQ=60)
                                      AGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGC                                                         >  SRR4302543.1867507/1‑94 (MQ=60)
                                      AGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGC                                                         <  SRR4302543.1771671/94‑1 (MQ=60)
                                       GCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCA                                                        >  SRR4302543.2951114/1‑94 (MQ=60)
                                        CTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAA                                                       <  SRR4302543.3518803/94‑1 (MQ=60)
                                          GCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGA                                                     >  SRR4302543.2204739/1‑94 (MQ=60)
                                           CAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGAC                                                    >  SRR4302543.2939618/1‑94 (MQ=60)
                                           CAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGAC                                                    >  SRR4302543.4274211/1‑94 (MQ=60)
                                            AAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACT                                                   <  SRR4302543.7017448/94‑1 (MQ=60)
                                              AATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTT                                                 <  SRR4302543.6682322/94‑1 (MQ=60)
                                              AATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTT                                                 <  SRR4302543.5795600/94‑1 (MQ=60)
                                               ATGATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTG                                                >  SRR4302543.7834657/1‑94 (MQ=60)
                                               ATAATCGCTTTCCCGCCAGGGCGCAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTG                                                >  SRR4302543.4495969/1‑94 (MQ=60)
                                                TAATCGCTTTCCCGCCCGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGgcggtgaccaatggcaacactttgg                                               >  SRR4302543.6024789/1‑69 (MQ=60)
                                                TAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGC                                               >  SRR4302543.6024788/1‑94 (MQ=60)
                                                 AATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCA                                              >  SRR4302543.2543605/1‑94 (MQ=60)
                                                 AATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCA                                              <  SRR4302543.6973841/94‑1 (MQ=60)
                                                  ATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAG                                             >  SRR4302543.212740/1‑94 (MQ=60)
                                                  ATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAG                                             >  SRR4302543.212739/1‑94 (MQ=60)
                                                    CGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCG                                           <  SRR4302543.247203/94‑1 (MQ=60)
                                                     GCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGT                                          >  SRR4302543.5558747/1‑94 (MQ=60)
                                                     GCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGAAGCGT                                          <  SRR4302543.2881819/94‑1 (MQ=60)
                                                      CTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTC                                         <  SRR4302543.5944706/94‑1 (MQ=60)
                                                        TTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCAT                                       <  SRR4302543.3688263/94‑1 (MQ=60)
                                                        TACCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCAT                                       <  SRR4302543.7974097/94‑1 (MQ=60)
                                                         TCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATC                                      <  SRR4302543.2715636/94‑1 (MQ=60)
                                                         TCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATC                                      >  SRR4302543.5957671/1‑94 (MQ=60)
                                                         TCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCAATGGCAAGACTTTGCAGCGTCATC                                      <  SRR4302543.1420749/94‑1 (MQ=60)
                                                          CCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGTAGCGTCATCA                                     <  SRR4302543.1909242/94‑1 (MQ=60)
                                                          CCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCA                                     <  SRR4302543.6406225/94‑1 (MQ=60)
                                                          CCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCA                                     <  SRR4302543.446134/94‑1 (MQ=60)
                                                          CCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCA                                     >  SRR4302543.791940/1‑94 (MQ=60)
                                                           CCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAG                                    <  SRR4302543.2120867/94‑1 (MQ=60)
                                                           CCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAG                                    <  SRR4302543.6449491/94‑1 (MQ=60)
                                                            CGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGC                                   >  SRR4302543.6679820/1‑94 (MQ=60)
                                                             GCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCG                                  >  SRR4302543.3456444/1‑94 (MQ=60)
                                                             GCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAAGACCACTGGCAAGACTTTGCAGCGTCATCAGCG                                  <  SRR4302543.4187130/94‑1 (MQ=60)
                                                               CAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGA                                >  SRR4302543.5603208/1‑94 (MQ=60)
                                                                AGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAT                               <  SRR4302543.6638233/94‑1 (MQ=60)
                                                                AGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAT                               <  SRR4302543.6391791/94‑1 (MQ=60)
                                                                 GGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATC                              <  SRR4302543.4500356/94‑1 (MQ=60)
                                                                 GGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATC                              >  SRR4302543.6971252/1‑94 (MQ=60)
                                                                 GGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATC                              <  SRR4302543.5174258/94‑1 (MQ=60)
                                                                  GTCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCG                             <  SRR4302543.3067924/94‑1 (MQ=60)
                                                                  GGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCG                             >  SRR4302543.5897888/1‑94 (MQ=60)
                                                                    CGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGT                           >  SRR4302543.7049372/1‑94 (MQ=60)
                                                                    CGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGT                           >  SRR4302543.3386200/1‑94 (MQ=60)
                                                                     GTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTC                          >  SRR4302543.25596/1‑94 (MQ=60)
                                                                     GTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTC                          >  SRR4302543.1945588/1‑94 (MQ=60)
                                                                      TAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCA                         <  SRR4302543.304721/94‑1 (MQ=60)
                                                                         AGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCG                      <  SRR4302543.4696579/94‑1 (MQ=60)
                                                                           ccagggGATAATCACCGAGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACGTTGCAGCGTCATCAGCGGATCGGTCAGCGGA                    <  SRR4302543.7516579/88‑1 (MQ=60)
                                                                           CACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGA                    <  SRR4302543.6230627/94‑1 (MQ=60)
                                                                             CGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAAC                  >  SRR4302543.3417420/1‑94 (MQ=60)
                                                                                TGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGT               <  SRR4302543.3840912/94‑1 (MQ=60)
                                                                                TGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGT               >  SRR4302543.4502671/1‑94 (MQ=60)
                                                                                TGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGG               >  SRR4302543.7365197/1‑94 (MQ=60)
                                                                                GGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGT               <  SRR4302543.1653785/94‑1 (MQ=60)
                                                                                GGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGT               <  SRR4302543.1856297/94‑1 (MQ=60)
                                                                                 GATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTG              <  SRR4302543.1304618/94‑1 (MQ=60)
                                                                                  ATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGT             >  SRR4302543.5014457/1‑94 (MQ=60)
                                                                                  ATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGT             <  SRR4302543.239340/94‑1 (MQ=60)
                                                                                  ATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAACGGAACGGTGT             >  SRR4302543.2203584/1‑94 (MQ=60)
                                                                                    AATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCG           >  SRR4302543.852976/1‑94 (MQ=60)
                                                                                    AATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCG           <  SRR4302543.7960577/94‑1 (MQ=60)
                                                                                     ATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGA          >  SRR4302543.7123428/1‑94 (MQ=60)
                                                                                     ATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGA          >  SRR4302543.5571631/1‑94 (MQ=60)
                                                                                      TCCCCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAA         <  SRR4302543.5853650/94‑1 (MQ=60)
                                                                                      TCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAA         <  SRR4302543.4790995/94‑1 (MQ=60)
                                                                                        CCCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATA       <  SRR4302543.4348139/94‑1 (MQ=60)
                                                                                        CCCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATA       <  SRR4302543.5435222/94‑1 (MQ=60)
                                                                                         CCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAA      <  SRR4302543.703756/94‑1 (MQ=60)
                                                                                         CCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAA      >  SRR4302543.6261061/1‑94 (MQ=60)
                                                                                           GCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACA    <  SRR4302543.5680137/94‑1 (MQ=60)
                                                                                           GCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACA    <  SRR4302543.6306165/94‑1 (MQ=60)
                                                                                           GCGCCGGTCAACGACCCCGTGGCAGGCAGATGACCACTGGCTAGACTTTGCAGCGTCATCCGCGGATCGGTCAGCGGAACGGTGTCGCATACCA    <  SRR4302543.7104634/94‑1 (MQ=60)
                                                                                            CGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAG   <  SRR4302543.7660140/94‑1 (MQ=60)
                                                                                             GCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAGG  <  SRR4302543.7763584/94‑1 (MQ=60)
                                                                                             GCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAGG  <  SRR4302543.7763586/94‑1 (MQ=60)
                                                                                             GCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAGG  <  SRR4302543.2758688/94‑1 (MQ=60)
                                                                                             GCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAGG  <  SRR4302543.5696662/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTTCGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAGG  >  NC_000913/2297242‑2297428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑