Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,298,561 T→C H738R (CAC→CGC)  napA ← putative nitrate reductase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,298,5610TC100.0% 710.7 / NA 228H738R (CAC→CGC) napAputative nitrate reductase 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (112/116);  total (112/116)

Only 100 of 238 total aligned reads displayed.

GAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGTGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCC  >  NC_000913/2298471‑2298650
                                                                                          |                                                                                         
  aaCCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAg                                                                                       <  2:6928127/93‑1 (MQ=255)
   aCCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGt                                                                                      <  1:6091113/93‑1 (MQ=255)
    ccACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGtt                                                                                     >  2:4099292/1‑93 (MQ=255)
    ccACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGtt                                                                                     <  2:7741234/93‑1 (MQ=255)
    ccACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGtt                                                                                     >  1:7168521/1‑93 (MQ=255)
     cactttcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTc                                                                                    <  1:5894909/93‑1 (MQ=255)
       ctttcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTg                                                                                  >  1:1964730/1‑93 (MQ=255)
        tttcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTgg                                                                                 <  1:164133/93‑1 (MQ=255)
        tttcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTgg                                                                                 <  2:1992180/93‑1 (MQ=255)
        tttcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTgg                                                                                 >  2:6835162/1‑93 (MQ=255)
          tcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTa                                                                               >  2:6022554/1‑93 (MQ=255)
          tcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTa                                                                               <  2:3458935/93‑1 (MQ=255)
          tcactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTa                                                                               <  2:363622/93‑1 (MQ=255)
           cactttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTac                                                                              <  1:3669626/93‑1 (MQ=255)
             ctttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTacac                                                                            <  2:7509363/93‑1 (MQ=255)
             ctttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTacac                                                                            >  1:3479234/1‑93 (MQ=255)
              tttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACg                                                                           >  2:4832953/1‑93 (MQ=255)
               ttGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACgg                                                                          >  1:1828811/1‑93 (MQ=255)
                  tCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGa                                                                       >  2:3790637/1‑93 (MQ=255)
                  tCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGa                                                                       <  1:7327082/93‑1 (MQ=255)
                  tCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGa                                                                       <  1:6873477/93‑1 (MQ=255)
                    gCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGt                                                                     >  1:5807118/1‑93 (MQ=255)
                      cACGGCTCAGATCGCGCGCTTTCACATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCa                                                                   <  2:5409573/93‑1 (MQ=255)
                      cACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCa                                                                   <  2:653751/93‑1 (MQ=255)
                       aCGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCAt                                                                  >  2:266346/1‑93 (MQ=255)
                       aCGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCAt                                                                  <  2:3264289/93‑1 (MQ=255)
                        cGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATa                                                                 <  2:4765465/93‑1 (MQ=255)
                         ggCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAGGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATAc                                                                <  2:6637934/93‑1 (MQ=255)
                         ggCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGCCATAc                                                                <  2:5048821/93‑1 (MQ=255)
                         ggCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTCCTGGCACACGGCGAGTCATAc                                                                <  1:3951470/93‑1 (MQ=255)
                          gcgcAGATGGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACt                                                               <  2:1321065/93‑1 (MQ=255)
                            gcAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGc                                                             <  1:3433486/93‑1 (MQ=255)
                             cAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGcc                                                            <  1:2179522/93‑1 (MQ=255)
                             cAGATCGCGCGATTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGcc                                                            <  1:7478532/93‑1 (MQ=255)
                              aGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCg                                                           >  2:2244528/1‑93 (MQ=255)
                              aGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCg                                                           <  1:5166334/93‑1 (MQ=255)
                               gATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCgg                                                          >  1:1443491/1‑93 (MQ=255)
                               gATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCgg                                                          >  1:125688/1‑93 (MQ=255)
                                 tCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGgtg                                                        <  2:1031879/93‑1 (MQ=255)
                                 tCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGgtg                                                        <  2:4865187/93‑1 (MQ=255)
                                 tCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGgtg                                                        >  2:2957173/1‑93 (MQ=255)
                                  cgcgcgCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGgtgt                                                       >  2:4378743/1‑93 (MQ=255)
                                     gcgcTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGcc                                                    >  2:553724/1‑93 (MQ=255)
                                      cgcTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCa                                                   <  2:6068009/93‑1 (MQ=255)
                                       gcTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAg                                                  >  2:7113958/1‑93 (MQ=255)
                                        cTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAgg                                                 >  2:458021/1‑92 (MQ=255)
                                        cTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGt                                                 >  2:5004369/1‑93 (MQ=255)
                                        cTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGt                                                 >  1:6299434/1‑93 (MQ=255)
                                        cTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGt                                                 <  2:5847030/93‑1 (MQ=255)
                                        cTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGt                                                 >  1:4261076/1‑93 (MQ=255)
                                        cTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGt                                                 <  1:5531128/93‑1 (MQ=255)
                                         tttCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTg                                                >  1:3817110/1‑93 (MQ=255)
                                          ttCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGc                                               <  1:3897270/93‑1 (MQ=255)
                                          ttCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGc                                               <  1:1491637/93‑1 (MQ=255)
                                           tCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCt                                              >  1:6644701/1‑93 (MQ=255)
                                             gCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTcc                                            <  1:300588/93‑1 (MQ=255)
                                             gCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTcc                                            >  2:5309528/1‑93 (MQ=255)
                                                tCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGa                                         >  2:7717507/1‑93 (MQ=255)
                                                   aGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAAcg                                      >  2:5782269/1‑93 (MQ=255)
                                                   aGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAAcg                                      >  2:3362504/1‑93 (MQ=255)
                                                      gggTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGt                                   >  2:5920554/1‑93 (MQ=255)
                                                      gggTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGt                                   <  1:905769/93‑1 (MQ=255)
                                                        gTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTcc                                 >  1:3597477/1‑93 (MQ=255)
                                                         tGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCg                                >  1:327031/1‑93 (MQ=255)
                                                         tGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCg                                >  1:3338504/1‑93 (MQ=255)
                                                          gAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCgg                               >  1:2580291/1‑93 (MQ=255)
                                                           aaTAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGt                              >  1:6815433/1‑93 (MQ=255)
                                                           aaTAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGt                              >  1:111929/1‑93 (MQ=255)
                                                            aTAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTa                             >  2:4353077/1‑93 (MQ=255)
                                                             tAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTag                            <  1:7692941/93‑1 (MQ=255)
                                                              aaaCAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTaga                           <  2:850913/93‑1 (MQ=255)
                                                              aaaCAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTaga                           >  2:7932292/1‑93 (MQ=255)
                                                                aCAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTagaga                         <  1:5756142/93‑1 (MQ=255)
                                                                 cAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTagagag                        <  2:3336468/93‑1 (MQ=255)
                                                                 cAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTagagag                        >  1:447571/1‑93 (MQ=255)
                                                                    gACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCca                     >  2:3095002/1‑93 (MQ=255)
                                                                       cGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATCCTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCTGTAGAGAGCCACAg                  <  2:6686811/93‑1 (MQ=255)
                                                                       cGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAg                  <  2:4032726/93‑1 (MQ=255)
                                                                       cGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAg                  <  2:7305553/93‑1 (MQ=255)
                                                                        gCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAgg                 <  1:4532624/93‑1 (MQ=255)
                                                                        gCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAgg                 >  2:6704808/1‑93 (MQ=255)
                                                                        gCTTCCGGGAAGGCGCGGCGCAGTTCTAGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAgg                 <  1:2473379/93‑1 (MQ=255)
                                                                         cTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGt                <  1:2733673/93‑1 (MQ=255)
                                                                           tCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCg              >  1:6990313/1‑93 (MQ=255)
                                                                             cGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTa            <  2:4435648/93‑1 (MQ=255)
                                                                             cGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTa            <  1:843312/93‑1 (MQ=255)
                                                                             cGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTa            <  2:2582550/93‑1 (MQ=255)
                                                                             cGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACCGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTa            <  2:2192142/93‑1 (MQ=255)
                                                                              gggAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTAc           <  2:644413/93‑1 (MQ=255)
                                                                               ggAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTAct          >  2:5091076/1‑93 (MQ=255)
                                                                               ggAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTAct          >  1:2328143/1‑93 (MQ=255)
                                                                                gAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTActc         <  2:6303466/93‑1 (MQ=255)
                                                                                gAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTActc         >  1:2674089/1‑93 (MQ=255)
                                                                                gAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTActc         <  1:1162393/93‑1 (MQ=255)
                                                                                 aaGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTActct        >  2:6560400/1‑93 (MQ=255)
                                                                                  aGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCtt       <  2:1664576/93‑1 (MQ=255)
                                                                                  aGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCtt       <  2:2699143/93‑1 (MQ=255)
                                                                                    gcgcGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCa     <  2:177390/93‑1 (MQ=255)
                                                                                     cgcgGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCAt    <  2:1124373/93‑1 (MQ=255)
                                                                                       cgGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATcc  >  1:5105576/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGTGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCC  >  NC_000913/2298471‑2298650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGTGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCCGGTG  >  NC_000913/2298468‑2298654
                                                                                             |                                                                                             
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGC                                                                                               >  SRR4302543.5191837/1‑94 (MQ=60)
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGC                                                                                               >  SRR4302543.6024509/1‑94 (MQ=60)
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGC                                                                                               <  SRR4302543.7255045/94‑1 (MQ=60)
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGC                                                                                               >  SRR4302543.7009887/1‑94 (MQ=60)
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGC                                                                                               >  SRR4302543.1537404/1‑94 (MQ=60)
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCAAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGC                                                                                               >  SRR4302543.4338467/1‑94 (MQ=60)
  AGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGC                                                                                             >  SRR4302543.6288947/1‑94 (MQ=60)
      ACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTT                                                                                         >  SRR4302543.7238140/1‑94 (MQ=60)
      ACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTT                                                                                         <  SRR4302543.6148845/94‑1 (MQ=60)
       CCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTC                                                                                        >  SRR4302543.5871039/1‑94 (MQ=60)
        CACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCT                                                                                       <  SRR4302543.5948663/94‑1 (MQ=60)
         ACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTG                                                                                      <  SRR4302543.4525312/94‑1 (MQ=60)
         ACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTG                                                                                      >  SRR4302543.1982353/1‑94 (MQ=60)
          CTTTCTCTTTGTCGCCACGGCGCAGATCGCGCGCTTTCTCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGG                                                                                     <  SRR4302543.7264797/94‑1 (MQ=60)
          CTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGGGGCGCAGTTCTGG                                                                                     >  SRR4302543.224433/1‑94 (MQ=60)
          CTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGG                                                                                     <  SRR4302543.252661/94‑1 (MQ=60)
           TTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGT                                                                                    <  SRR4302543.165547/94‑1 (MQ=60)
           TTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGT                                                                                    <  SRR4302543.992704/94‑1 (MQ=60)
              CACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACA                                                                                 <  SRR4302543.3701847/94‑1 (MQ=60)
              CACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACA                                                                                 <  SRR4302543.3701846/94‑1 (MQ=60)
               ACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACAC                                                                                >  SRR4302543.3510398/1‑94 (MQ=60)
                 TTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGG                                                                              >  SRR4302543.1845238/1‑94 (MQ=60)
                 TTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAACGCGCGGCGCAGTTCTGGTACACGG                                                                              >  SRR4302543.119725/1‑94 (MQ=60)
                   TGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCG                                                                            >  SRR4302543.2050651/1‑94 (MQ=60)
                   TGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCG                                                                            <  SRR4302543.5490145/94‑1 (MQ=60)
                     TCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAG                                                                          <  SRR4302543.3908671/94‑1 (MQ=60)
                     TCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAG                                                                          <  SRR4302543.6938198/94‑1 (MQ=60)
                     TCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAG                                                                          <  SRR4302543.7397622/94‑1 (MQ=60)
                      CGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGT                                                                         >  SRR4302543.5859936/1‑94 (MQ=60)
                      CGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGT                                                                         >  SRR4302543.2584801/1‑94 (MQ=60)
                      CGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGT                                                                         <  SRR4302543.471960/94‑1 (MQ=60)
                      CGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGC                                                                         >  SRR4302543.4152176/1‑94 (MQ=60)
                        CCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCA                                                                       >  SRR4302543.3392074/1‑94 (MQ=60)
                        CCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGAGAATAAACAGGACAGCTTCCGGGAAGGCGCGGCGCAGATCTGGTACACGGCGAGTCA                                                                       >  SRR4302543.7903516/1‑94 (MQ=60)
                         CACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCAT                                                                      >  SRR4302543.4003489/1‑94 (MQ=60)
                         CACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGAGGCGCAGTTCTGGTACACGGCGAGCCAT                                                                      >  SRR4302543.531255/1‑94 (MQ=60)
                            GGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCGGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACT                                                                   <  SRR4302543.6846219/94‑1 (MQ=60)
                            GGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACT                                                                   >  SRR4302543.1563251/1‑94 (MQ=60)
                            GGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTCCTGGCACACGGCGAGTCATACT                                                                   <  SRR4302543.3986426/94‑1 (MQ=60)
                            GGCCCAGATCGCGCGCTTTCGCATCAGGCGGGTGAATAACCAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACCCGGCGAGTCATACT                                                                   <  SRR4302543.3964504/94‑1 (MQ=60)
                             GCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGCGTCATACTG                                                                  >  SRR4302543.3363597/1‑94 (MQ=60)
                             GCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTG                                                                  >  SRR4302543.3363599/1‑94 (MQ=60)
                             GCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTG                                                                  <  SRR4302543.3879608/94‑1 (MQ=60)
                              CGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGC                                                                 >  SRR4302543.1338155/1‑94 (MQ=60)
                               GCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCC                                                                <  SRR4302543.3464213/94‑1 (MQ=60)
                               GCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCC                                                                >  SRR4302543.1697251/1‑94 (MQ=60)
                                CAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCG                                                               <  SRR4302543.2199063/94‑1 (MQ=60)
                                CAGATCGCGCGATTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCG                                                               <  SRR4302543.7550122/94‑1 (MQ=60)
                                 AGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGG                                                              >  SRR4302543.126824/1‑94 (MQ=60)
                                 AGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGG                                                              >  SRR4302543.1456532/1‑94 (MQ=60)
                                 AGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGG                                                              <  SRR4302543.5213012/94‑1 (MQ=60)
                                   ATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTG                                                            >  SRR4302543.13623/1‑94 (MQ=60)
                                     CGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTG                                                          <  SRR4302543.2385112/94‑1 (MQ=60)
                                      GCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGC                                                         <  SRR4302543.650603/94‑1 (MQ=60)
                                       CGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCC                                                        >  SRR4302543.511268/1‑94 (MQ=60)
                                        GCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCA                                                       <  SRR4302543.273231/94‑1 (MQ=60)
                                         CGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAG                                                      <  SRR4302543.7297339/94‑1 (MQ=60)
                                          GCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGT                                                     >  SRR4302543.6358716/1‑94 (MQ=60)
                                          GCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGT                                                     <  SRR4302543.5835381/94‑1 (MQ=60)
                                          GCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGT                                                     >  SRR4302543.4299780/1‑94 (MQ=60)
                                           CTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTG                                                    <  SRR4302543.5581382/94‑1 (MQ=60)
                                           CTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTG                                                    >  SRR4302543.3850766/1‑94 (MQ=60)
                                             TTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCT                                                  >  SRR4302543.6707416/1‑94 (MQ=60)
                                             TTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCT                                                  <  SRR4302543.1505059/94‑1 (MQ=60)
                                             TTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCT                                                  <  SRR4302543.3931689/94‑1 (MQ=60)
                                              TCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTC                                                 >  SRR4302543.4406964/1‑94 (MQ=60)
                                               CGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCC                                                >  SRR4302543.6614441/1‑94 (MQ=60)
                                               CGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCC                                                >  SRR4302543.2907999/1‑94 (MQ=60)
                                                GCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCA                                               <  SRR4302543.303289/94‑1 (MQ=60)
                                                GCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCA                                               <  SRR4302543.5931377/94‑1 (MQ=60)
                                                GCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCA                                               <  SRR4302543.3503440/94‑1 (MQ=60)
                                                  ATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGA                                             >  SRR4302543.6050237/1‑94 (MQ=60)
                                                   TCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGtaaaagggcgggcctacctcccggggggccagggctccagac                                            >  SRR4302543.6748494/1‑52 (MQ=60)
                                                    CCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAAC                                           >  SRR4302543.3528310/1‑94 (MQ=60)
                                                    CCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAAC                                           <  SRR4302543.7189855/94‑1 (MQ=60)
                                                    CCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAAC                                           >  SRR4302543.7632603/1‑94 (MQ=60)
                                                       GCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCG                                        <  SRR4302543.2180824/94‑1 (MQ=60)
                                                        CGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGT                                       >  SRR4302543.747202/1‑94 (MQ=60)
                                                         GGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTC                                      >  SRR4302543.3230162/1‑94 (MQ=60)
                                                         GGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTC                                      <  SRR4302543.913743/94‑1 (MQ=60)
                                                          GGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCC                                     >  SRR4302543.3629226/1‑94 (MQ=60)
                                                          GGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCC                                     <  SRR4302543.56550/94‑1 (MQ=60)
                                                           GTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCG                                    >  SRR4302543.330001/1‑94 (MQ=60)
                                                           GTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCG                                    >  SRR4302543.1862212/1‑94 (MQ=60)
                                                           GTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCG                                    >  SRR4302543.3368581/1‑94 (MQ=60)
                                                            TGAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGG                                   >  SRR4302543.2603788/1‑94 (MQ=60)
                                                             GAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGT                                  >  SRR4302543.6879774/1‑94 (MQ=60)
                                                             GAATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGT                                  >  SRR4302543.112946/1‑94 (MQ=60)
                                                              AATAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTA                                 >  SRR4302543.1147388/1‑94 (MQ=60)
                                                                TAAACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGA                               <  SRR4302543.7765772/94‑1 (MQ=60)
                                                                   ACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAG                            >  SRR4302543.289805/1‑94 (MQ=60)
                                                                   ACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAG                            >  SRR4302543.961860/1‑94 (MQ=60)
                                                                   ACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAG                            <  SRR4302543.5808595/94‑1 (MQ=60)
                                                                   ACAGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAG                            >  SRR4302543.451757/1‑94 (MQ=60)
                                                                     AGGACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCC                          >  SRR4302543.6931356/1‑94 (MQ=60)
                                                                        ACCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACA                       >  SRR4302543.5195838/1‑94 (MQ=60)
                                                                         CCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAG                      >  SRR4302543.1499994/1‑94 (MQ=60)
                                                                         CCGCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAG                      <  SRR4302543.869580/94‑1 (MQ=60)
                                                                           GCTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGT                    <  SRR4302543.4573881/94‑1 (MQ=60)
                                                                           GCTTCCGGGAAGGCGCGGCGCAGTTCTAGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGT                    <  SRR4302543.2495724/94‑1 (MQ=60)
                                                                            CTTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTC                   <  SRR4302543.2758550/94‑1 (MQ=60)
                                                                             TTCCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCG                  >  SRR4302543.7056102/1‑94 (MQ=60)
                                                                               CCGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTA                >  SRR4302543.6998944/1‑94 (MQ=60)
                                                                                CGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTAC               >  SRR4302543.6931527/1‑94 (MQ=60)
                                                                                CGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTAC               <  SRR4302543.850772/94‑1 (MQ=60)
                                                                                CGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTAC               >  SRR4302543.3681466/1‑94 (MQ=60)
                                                                                CGGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGCCGTAC               >  SRR4302543.358983/1‑94 (MQ=60)
                                                                                 GGGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACT              >  SRR4302543.2349286/1‑94 (MQ=60)
                                                                                  GGAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTC             >  SRR4302543.2698332/1‑94 (MQ=60)
                                                                                  GGAAGGCGCGGCGCAGTTCTGGTACACGGAGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGCGAGCCACAGGTCGTAatt             >  SRR4302543.2698331/1‑91 (MQ=60)
                                                                                   GAAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCT            <  SRR4302543.1172976/94‑1 (MQ=60)
                                                                                    AAGGCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTT           <  SRR4302543.1665673/94‑1 (MQ=60)
                                                                                       GCGCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCAT        >  SRR4302543.7814748/1‑94 (MQ=60)
                                                                                         GCGGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCC      >  SRR4302543.5151778/1‑94 (MQ=60)
                                                                                           GGCGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCCGG    <  SRR4302543.2700847/94‑1 (MQ=60)
                                                                                             CGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCCGGTG  >  SRR4302543.1002006/1‑94 (MQ=60)
                                                                                             CGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCCGGTG  >  SRR4302543.2246381/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGAGAAACCACTTTCACTTTGTCGCCACGGCGCAGATCGCGCGCTTTCGCATCCAGCGGGTGAATAAACAGGACCGCTTCCGGGAAGGCGCGGTGCAGTTCTGGTACACGGCGAGTCATACTGCCGGTGTGCCAGTGCTCCAGAACGCGTCCGGTAGAGAGCCACAGGTCGTACTCTTCATCCGGTG  >  NC_000913/2298468‑2298654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑