Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,299,266 T→C N503S (AAC→AGC)  napA ← putative nitrate reductase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,299,2660TC100.0% 577.1 / NA 182N503S (AAC→AGC) napAputative nitrate reductase 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (78/104);  total (78/104)

Only 100 of 198 total aligned reads displayed.

CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355
                                                                                          |                                                                                         
cccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTg                                                                                         >  2:2826788/1‑93 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgc                                                                                        >  2:1699465/1‑93 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgc                                                                                        >  1:1460319/1‑93 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgc                                                                                        <  2:450570/93‑1 (MQ=255)
  ctctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcg                                                                                       >  1:4230028/1‑93 (MQ=255)
  ctctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcg                                                                                       <  2:4411863/93‑1 (MQ=255)
   tgtttCGCTTCCCACACTGCGTTCGTCAGGATCAAGCCCGCGGCCAGCGCACTGTCTGTCGGATACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      <  1:7477323/91‑1 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGCTACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      >  1:1664946/1‑93 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      >  1:4838308/1‑93 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGCCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      >  1:6785980/1‑93 (MQ=255)
    ctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGcgcg                                                                                     <  1:1391905/93‑1 (MQ=255)
     tttCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCCGCGACGATTATGAAGCTGcgcga                                                                                    >  1:72972/1‑92 (MQ=255)
       tctcTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAt                                                                                  >  1:788953/1‑93 (MQ=255)
       tctcTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAt                                                                                  <  2:771106/93‑1 (MQ=255)
       tctcTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAt                                                                                  <  1:962327/93‑1 (MQ=255)
        ctctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATc                                                                                 <  2:882517/93‑1 (MQ=255)
           tACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcgcg                                                                              >  2:6416948/1‑93 (MQ=255)
            aCCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCgcgc                                                                             >  2:1564242/1‑93 (MQ=255)
            aCCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCgcgc                                                                             >  2:1462149/1‑93 (MQ=255)
             cccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGcc                                                                            <  1:7778287/93‑1 (MQ=255)
               cacaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAg                                                                          >  2:3256398/1‑93 (MQ=255)
                acaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGc                                                                         >  2:2026282/1‑93 (MQ=255)
                acaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGc                                                                         >  1:2798018/1‑93 (MQ=255)
                acaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGc                                                                         >  1:5035279/1‑93 (MQ=255)
                 caTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGcc                                                                        <  1:4388127/93‑1 (MQ=255)
                  aTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGccc                                                                       >  2:5015550/1‑93 (MQ=255)
                   ttGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCg                                                                      >  2:1734567/1‑93 (MQ=255)
                   ttGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCg                                                                      <  2:6707916/93‑1 (MQ=255)
                    tGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCgg                                                                     <  2:49006/93‑1 (MQ=255)
                      cGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCa                                                                   <  2:337742/93‑1 (MQ=255)
                      cGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCa                                                                   <  1:5870348/93‑1 (MQ=255)
                       ggTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAt                                                                  >  1:3321609/1‑93 (MQ=255)
                       ggTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAt                                                                  >  2:1275713/1‑93 (MQ=255)
                        gTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATa                                                                 >  1:1093929/1‑93 (MQ=255)
                        gTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATa                                                                 <  1:6352072/93‑1 (MQ=255)
                         tCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAc                                                                >  1:7092587/1‑93 (MQ=255)
                         tCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAc                                                                >  1:998680/1‑93 (MQ=255)
                           ggCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGc                                                              <  1:6838147/93‑1 (MQ=255)
                            gCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGct                                                             >  1:3393863/1‑93 (MQ=255)
                            gCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCCGATCGCGCCAGCCCGGCATACGct                                                             >  1:3418705/1‑93 (MQ=255)
                              aGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctct                                                           >  1:4765829/1‑93 (MQ=255)
                               ggATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCtt                                                          >  1:5980832/1‑93 (MQ=255)
                                 aTCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCa                                                        <  2:6675299/93‑1 (MQ=255)
                                 aTCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCa                                                        <  2:6095859/93‑1 (MQ=255)
                                  tCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGCCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAt                                                       <  2:4018753/93‑1 (MQ=255)
                                  tCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAt                                                       >  2:1307414/1‑93 (MQ=255)
                                   cAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAtt                                                      >  2:7851993/1‑93 (MQ=255)
                                   cAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAtt                                                      <  1:1573953/93‑1 (MQ=255)
                                     aGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGa                                                    >  2:4150243/1‑93 (MQ=255)
                                     aGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGa                                                    <  2:4749373/93‑1 (MQ=255)
                                      gTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAt                                                   <  1:754796/93‑1 (MQ=255)
                                         ggcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGtt                                                <  1:4090056/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  1:5776532/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  2:5731999/93‑1 (MQ=255)
                                                aGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccg                                         >  1:7172651/1‑93 (MQ=255)
                                                  ggccctgactgTCGGATAAGGAACGCAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcgcggc                                       <  1:6568179/89‑1 (MQ=255)
                                                   gcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggcc                                      >  1:2471495/1‑93 (MQ=255)
                                                   gcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggcc                                      >  1:2627239/1‑93 (MQ=255)
                                                      ctgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGc                                   >  2:7070063/1‑93 (MQ=255)
                                                        gactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAt                                 <  1:2132716/93‑1 (MQ=255)
                                                          ctgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgt                               <  1:1053386/93‑1 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              >  1:2083803/1‑93 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              >  2:1548338/1‑93 (MQ=255)
                                                            ttaggATACGGATCGGAGACGATGATGAAGCTGGGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             <  2:4218413/90‑1 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             <  1:5699574/93‑1 (MQ=255)
                                                               ggATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGttgttg                          <  1:7744835/93‑1 (MQ=255)
                                                               ggATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGttgttg                          <  2:2325329/93‑1 (MQ=255)
                                                                gATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGATGTTgg                         >  1:2370539/1‑93 (MQ=255)
                                                                 aTACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGt                        >  2:6984630/1‑93 (MQ=255)
                                                                  tACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGGTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTa                       <  2:6617306/93‑1 (MQ=255)
                                                                    cGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTaca                     <  2:7323968/93‑1 (MQ=255)
                                                                      gATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAcaca                   <  2:6035387/93‑1 (MQ=255)
                                                                      gATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAcaca                   >  1:6561057/1‑93 (MQ=255)
                                                                       aTCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAt                  <  2:5673280/93‑1 (MQ=255)
                                                                       aTCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAt                  <  2:1526255/93‑1 (MQ=255)
                                                                        tCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATg                 <  1:6085942/93‑1 (MQ=255)
                                                                        tCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATg                 >  1:7454177/1‑93 (MQ=255)
                                                                          ggAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGt               >  1:6918691/1‑93 (MQ=255)
                                                                          ggAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGt               <  1:5479726/93‑1 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              <  2:1024915/93‑1 (MQ=255)
                                                                            agaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCTGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTcc             >  1:1398072/1‑93 (MQ=255)
                                                                            agaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTcc             >  2:6791812/1‑93 (MQ=255)
                                                                             gaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            <  1:1728854/93‑1 (MQ=255)
                                                                             gaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            >  1:4544609/1‑93 (MQ=255)
                                                                             gaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            <  2:4134814/93‑1 (MQ=255)
                                                                             gaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            >  1:3639279/1‑93 (MQ=255)
                                                                              aCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAg           <  2:3690/93‑1 (MQ=255)
                                                                                 atgatgAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaa        <  1:1020171/93‑1 (MQ=255)
                                                                                 atgatgAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaa        >  2:2472040/1‑93 (MQ=255)
                                                                                  tgatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaac       >  1:3966479/1‑92 (MQ=255)
                                                                                  tgatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaaa       <  2:4755829/93‑1 (MQ=255)
                                                                                  tgatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaaa       >  1:5136379/1‑93 (MQ=255)
                                                                                    atgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACa     <  1:335287/93‑1 (MQ=255)
                                                                                     tgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAt    >  1:4696540/1‑93 (MQ=255)
                                                                                      gaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAtt   <  2:5183289/93‑1 (MQ=255)
                                                                                      gaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAtt   <  2:6853346/93‑1 (MQ=255)
                                                                                      gaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAtt   <  2:3119360/93‑1 (MQ=255)
                                                                                       aaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTc  <  1:2358307/93‑1 (MQ=255)
                                                                                       aaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTc  <  1:1857630/93‑1 (MQ=255)
                                                                                       aaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTc  <  2:4610541/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAG  >  NC_000913/2299173‑2299357
                                                                                             |                                                                                           
GCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGC                                                                                             >  SRR4302543.1587733/1‑94 (MQ=60)
GCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGC                                                                                             >  SRR4302543.1587732/1‑94 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            <  SRR4302543.7180005/94‑1 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            <  SRR4302543.6360251/94‑1 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            >  SRR4302543.5743920/1‑94 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            <  SRR4302543.6708724/94‑1 (MQ=60)
 CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGCCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                            >  SRR4302543.7635610/1‑94 (MQ=60)
  GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATTCGGATCGGAGACGATGATGAAGCTG                                                                                           <  SRR4302543.7754081/94‑1 (MQ=60)
   CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGC                                                                                          >  SRR4302543.69065/1‑94 (MQ=60)
   CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGC                                                                                          >  SRR4302543.7538845/1‑94 (MQ=60)
   CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGC                                                                                          >  SRR4302543.1473486/1‑94 (MQ=60)
    CCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCG                                                                                         <  SRR4302543.2467614/94‑1 (MQ=60)
    CCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCG                                                                                         >  SRR4302543.4268401/1‑94 (MQ=60)
     CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGCTACGGATCGGAGACGATGATGAAGCTGCGC                                                                                        >  SRR4302543.1679862/1‑94 (MQ=60)
     CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGC                                                                                        >  SRR4302543.4882433/1‑94 (MQ=60)
     CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGCCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGC                                                                                        >  SRR4302543.6850069/1‑94 (MQ=60)
      tgtttcgcttCCCACACTGCGTTCGTCAGGATCAAGCCCGCGGCCAGCGCACTGTCTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCG                                                                                       <  SRR4302543.7548904/84‑1 (MQ=58)
       CTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGG                                                                                      <  SRR4302543.1404439/94‑1 (MQ=60)
       CTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCCGCGACGATTATGAAGCTGCGCGA                                                                                      >  SRR4302543.73635/1‑94 (MQ=60)
       CTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGAACGGAGACGATGATGAATGTGCGCGG                                                                                      >  SRR4302543.73634/1‑94 (MQ=60)
         TTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAT                                                                                    >  SRR4302543.795892/1‑94 (MQ=60)
         TTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAT                                                                                    >  SRR4302543.2128093/1‑94 (MQ=60)
          TCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATC                                                                                   <  SRR4302543.970772/94‑1 (MQ=60)
                CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                             <  SRR4302543.7851640/94‑1 (MQ=60)
                 CCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAG                                                                            >  SRR4302543.4383746/1‑94 (MQ=60)
                  CACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGC                                                                           >  SRR4302543.5081082/1‑94 (MQ=60)
                  CACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGC                                                                           >  SRR4302543.2823594/1‑94 (MQ=60)
                    CATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC                                                                         <  SRR4302543.4427912/94‑1 (MQ=60)
                       TGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGC                                                                      <  SRR4302543.5868708/94‑1 (MQ=60)
                       TGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGC                                                                      <  SRR4302543.3386606/94‑1 (MQ=60)
                         CGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAT                                                                    <  SRR4302543.5923803/94‑1 (MQ=60)
                         CGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAT                                                                    >  SRR4302543.3351575/1‑94 (MQ=60)
                          GGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATA                                                                   >  SRR4302543.1103768/1‑94 (MQ=60)
                           GTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAC                                                                  <  SRR4302543.4122069/94‑1 (MQ=60)
                           GTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAC                                                                  <  SRR4302543.6839549/94‑1 (MQ=60)
                           GTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAC                                                                  >  SRR4302543.1007503/1‑94 (MQ=60)
                           GTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAC                                                                  <  SRR4302543.6411796/94‑1 (MQ=60)
                           GTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAC                                                                  <  SRR4302543.98542/94‑1 (MQ=60)
                           GTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAC                                                                  >  SRR4302543.7161473/1‑94 (MQ=60)
                            TCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCTCGTATCGCGCCAGCCCGGCATACG                                                                 <  SRR4302543.6534713/94‑1 (MQ=60)
                              GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGTTACGGATCGGAGACGATGATGAGGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                               >  SRR4302543.5119170/1‑94 (MQ=60)
                              GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                               <  SRR4302543.6902690/94‑1 (MQ=60)
                              GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                               >  SRR4302543.3424290/1‑94 (MQ=60)
                              GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCCGATCGCGCCAGCCCGGCATACGCT                                                               >  SRR4302543.3449303/1‑94 (MQ=60)
                              GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAACTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                               >  SRR4302543.4169227/1‑94 (MQ=60)
                                CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                             <  SRR4302543.3860099/94‑1 (MQ=60)
                                CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                             <  SRR4302543.7390561/94‑1 (MQ=60)
                                CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                             >  SRR4302543.4809386/1‑94 (MQ=60)
                                 AGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTT                                                            >  SRR4302543.6035628/1‑94 (MQ=60)
                                    ATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAT                                                         <  SRR4302543.1835727/94‑1 (MQ=60)
                                    ATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAT                                                         >  SRR4302543.5819106/1‑94 (MQ=60)
                                    ATCAAGCCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAT                                                         <  SRR4302543.7030255/94‑1 (MQ=60)
                                     TCAAGCCGGCGGACAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATT                                                        <  SRR4302543.6067555/94‑1 (MQ=60)
                                      CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTG                                                       <  SRR4302543.1587998/94‑1 (MQ=60)
                                        AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                     <  SRR4302543.2383843/94‑1 (MQ=60)
                                         GTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                    <  SRR4302543.761474/94‑1 (MQ=60)
                                          TCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGT                                                   >  SRR4302543.2762372/1‑94 (MQ=60)
                                            GGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTC                                                 <  SRR4302543.4126743/94‑1 (MQ=60)
                                             GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                <  SRR4302543.1682058/94‑1 (MQ=60)
                                             GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                <  SRR4302543.5693354/94‑1 (MQ=60)
                                             GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                <  SRR4302543.5829113/94‑1 (MQ=60)
                                             GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCCCTTCATTGATGTTCG                                                <  SRR4302543.7209176/94‑1 (MQ=60)
                                              CGGCCAGCTCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGG                                               <  SRR4302543.3355156/94‑1 (MQ=60)
                                              CGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGG                                               <  SRR4302543.4795398/94‑1 (MQ=60)
                                                GCCAGCGCACTGACTGTCGGATACGGATCGGAGACGGTGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCC                                             >  SRR4302543.1405636/1‑94 (MQ=60)
                                                GCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCC                                             >  SRR4302543.899484/1‑94 (MQ=60)
                                                GCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCC                                             >  SRR4302543.1439915/1‑94 (MQ=60)
                                                  CAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCG                                           >  SRR4302543.7242289/1‑94 (MQ=60)
                                                     ggccCTGACTGTCGGATAAGGAACGCAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCGCGGCC                                        <  SRR4302543.6630005/90‑1 (MQ=60)
                                                     CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                        >  SRR4302543.2493808/1‑94 (MQ=60)
                                                     CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                        >  SRR4302543.2651015/1‑94 (MQ=60)
                                                     CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                        <  SRR4302543.6629752/94‑1 (MQ=60)
                                                     CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCCGCCCGGCC                                        >  SRR4302543.200586/1‑94 (MQ=60)
                                                       CACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTG                                      >  SRR4302543.5127671/1‑94 (MQ=60)
                                                         CTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCA                                    >  SRR4302543.5326130/1‑94 (MQ=60)
                                                          TGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAT                                   <  SRR4302543.488170/94‑1 (MQ=60)
                                                           GACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATG                                  <  SRR4302543.2151810/94‑1 (MQ=60)
                                                           GACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATG                                  <  SRR4302543.2612564/94‑1 (MQ=60)
                                                             CTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTT                                >  SRR4302543.2102408/1‑94 (MQ=60)
                                                             CTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTT                                <  SRR4302543.1062831/94‑1 (MQ=60)
                                                              TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                               <  SRR4302543.3499782/94‑1 (MQ=60)
                                                               GTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGT                              <  SRR4302543.5751565/94‑1 (MQ=60)
                                                                 CGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTG                            <  SRR4302543.788958/94‑1 (MQ=60)
                                                                  GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                           <  SRR4302543.3433853/94‑1 (MQ=60)
                                                                  GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                           >  SRR4302543.7594644/1‑94 (MQ=60)
                                                                  GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                           <  SRR4302543.7817990/94‑1 (MQ=60)
                                                                  GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGATGTTGG                           >  SRR4302543.2392024/1‑94 (MQ=60)
                                                                      ACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACA                       >  SRR4302543.6278025/1‑94 (MQ=60)
                                                                        GGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACA                     >  SRR4302543.5941031/1‑94 (MQ=60)
                                                                        GGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACA                     >  SRR4302543.6622792/1‑94 (MQ=60)
                                                                         GATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAT                    <  SRR4302543.3262165/94‑1 (MQ=60)
                                                                          ATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATG                   >  SRR4302543.7525596/1‑94 (MQ=60)
                                                                          ATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATG                   <  SRR4302543.6854278/94‑1 (MQ=60)
                                                                           TCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGG                  <  SRR4302543.6143627/94‑1 (MQ=60)
                                                                            CGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGT                 >  SRR4302543.6983734/1‑94 (MQ=60)
                                                                             GGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTC                <  SRR4302543.5529388/94‑1 (MQ=60)
                                                                              GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCTGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC               >  SRR4302543.1410680/1‑94 (MQ=60)
                                                                               AGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCA              >  SRR4302543.4585994/1‑94 (MQ=60)
                                                                               AGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCA              >  SRR4302543.3671305/1‑94 (MQ=60)
                                                                                GACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAG             <  SRR4302543.1744265/94‑1 (MQ=60)
                                                                                  CGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTA           >  SRR4302543.1263773/1‑94 (MQ=60)
                                                                                    ATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAC         >  SRR4302543.4001621/1‑94 (MQ=60)
                                                                                    ATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAA         <  SRR4302543.1029200/94‑1 (MQ=60)
                                                                                    ATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAA         >  SRR4302543.5182811/1‑94 (MQ=60)
                                                                                    ATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAA         <  SRR4302543.106437/94‑1 (MQ=60)
                                                                                    ATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAA         <  SRR4302543.1678616/94‑1 (MQ=60)
                                                                                     TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC        <  SRR4302543.4481926/94‑1 (MQ=60)
                                                                                     TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC        >  SRR4302543.4633878/1‑94 (MQ=60)
                                                                                       ATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAT      >  SRR4302543.1523415/1‑94 (MQ=60)
                                                                                       ATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAT      <  SRR4302543.338366/94‑1 (MQ=60)
                                                                                       ATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAT      <  SRR4302543.7802033/94‑1 (MQ=60)
                                                                                       ATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAT      >  SRR4302543.4739428/1‑94 (MQ=60)
                                                                                         GAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC    <  SRR4302543.2179303/94‑1 (MQ=60)
                                                                                          AAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCA   <  SRR4302543.1874327/94‑1 (MQ=60)
                                                                                          AAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCA   <  SRR4302543.2379692/94‑1 (MQ=60)
                                                                                          AAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCA   <  SRR4302543.4952527/94‑1 (MQ=60)
                                                                                           AGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAG  <  SRR4302543.3269786/94‑1 (MQ=60)
                                                                                           AGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAG  <  SRR4302543.2740426/94‑1 (MQ=60)
                                                                                             |                                                                                           
GCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAG  >  NC_000913/2299173‑2299357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑