Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,996	G→A	D312N (GAC→AAC)	atoS →	AtoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,996	0	G	A	99.6%	844.5 / ‑5.7	233	D312N (GAC→AAC)	atoS	AtoS
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (112/120); total (112/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

Only 100 of 239 total aligned reads displayed.

GGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGAC  >  NC_000913/2318906‑2319085

 gTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACa                                                                                        <  1:5164650/93‑1 (MQ=255)

  tGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTcaacaa                                                                                       <  1:6489453/93‑1 (MQ=255)

  tGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTcaacaa                                                                                       <  1:6348390/93‑1 (MQ=255)

    aTGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATa                                                                                     >  2:6257310/1‑93 (MQ=255)

    aTGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATa                                                                                     >  2:3976344/1‑93 (MQ=255)

    aTGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATa                                                                                     <  1:1629381/93‑1 (MQ=255)

     tGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATAc                                                                                    >  1:7393824/1‑93 (MQ=255)

     tGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATAc                                                                                    >  1:3378936/1‑93 (MQ=255)

       tAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTc                                                                                  <  2:4713590/93‑1 (MQ=255)

       tAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTc                                                                                  >  1:2896473/1‑93 (MQ=255)

        aaCCACAATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCa                                                                                 <  1:2917124/93‑1 (MQ=255)

         accaccATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAg                                                                                <  2:2541343/93‑1 (MQ=255)

         accaccATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAg                                                                                >  2:1250915/1‑93 (MQ=255)

          ccaccaTGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGt                                                                               >  1:4101446/1‑93 (MQ=255)

           caccaTGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGtt                                                                              <  1:1940637/93‑1 (MQ=255)

           caccaTGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGtt                                                                              >  1:7917765/1‑93 (MQ=255)

             ccaTGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCt                                                                            <  2:269273/93‑1 (MQ=255)

              caTGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTa                                                                           >  2:3632890/1‑93 (MQ=255)

                tGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACa                                                                         <  1:6772630/93‑1 (MQ=255)

                tGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACa                                                                         <  2:6787510/93‑1 (MQ=255)

                tGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACa                                                                         <  2:4342372/93‑1 (MQ=255)

                   aCCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTc                                                                      >  2:1999634/1‑93 (MQ=255)

                    cccAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTcc                                                                     >  2:6203423/1‑93 (MQ=255)

                       agcagcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGt                                                                  <  1:6630212/93‑1 (MQ=255)

                        gcagcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTa                                                                 >  2:3323868/1‑93 (MQ=255)

                         cagcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTAc                                                                <  2:7159167/93‑1 (MQ=255)

                          agcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACt                                                               >  2:6073328/1‑93 (MQ=255)

                          agcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACt                                                               >  2:5319690/1‑93 (MQ=255)

                           gcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTg                                                              <  1:5987104/93‑1 (MQ=255)

                           gcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTg                                                              <  2:810200/93‑1 (MQ=255)

                             agAAGTTATCACTGGCTATCAACGCCATGAGCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGa                                                            <  2:4217772/93‑1 (MQ=255)

                             agAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGa                                                            <  2:7480863/93‑1 (MQ=255)

                             agAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGa                                                            >  2:6338117/1‑93 (MQ=255)

                             agAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGa                                                            <  1:5004490/93‑1 (MQ=255)

                              gAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGAt                                                           <  2:2995320/93‑1 (MQ=255)

                              gAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGAt                                                           >  1:3563379/1‑93 (MQ=255)

                               aaGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATa                                                          <  2:4612504/93‑1 (MQ=255)

                                 gTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACg                                                        >  1:2682428/1‑93 (MQ=255)

                                  ttATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTAGTGGCTaacc                                                       >  1:4286223/1‑90 (MQ=255)

                                  ttATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGc                                                       >  1:3459487/1‑93 (MQ=255)

                                  ttATCACTGGCTATCAACGCCATGAACTGGAAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGc                                                       >  2:1703887/1‑93 (MQ=255)

                                   tATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCt                                                      >  2:1369716/1‑93 (MQ=255)

                                   tATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCt                                                      >  2:2754619/1‑93 (MQ=255)

                                    aTCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTg                                                     >  1:6737514/1‑93 (MQ=255)

                                       aCTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATAGTCAGTTCTACAGTCCAGTACTGGATACGCTGGaa                                                  >  2:6575359/1‑93 (MQ=255)

                                        cTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                 >  1:2659214/1‑93 (MQ=255)

                                        cTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                 <  2:389373/93‑1 (MQ=255)

                                        cTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                 >  1:5349435/1‑93 (MQ=255)

                                        cTGGCTATCAACGCCATGAACTGCTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                 <  2:5653281/93‑1 (MQ=255)

                                         tGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACa                                                >  1:3802682/1‑93 (MQ=255)

                                         tGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACa                                                <  1:5917441/93‑1 (MQ=255)

                                           gCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATg                                              <  2:5951919/93‑1 (MQ=255)

                                           gCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATg                                              <  2:6898336/93‑1 (MQ=255)

                                               tCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCAc                                          >  1:2201750/1‑93 (MQ=255)

                                               tCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCAc                                          <  1:4460328/93‑1 (MQ=255)

                                                     ccATGATCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACa                                    <  1:5552988/93‑1 (MQ=255)

                                                     ccATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACa                                    <  2:6468936/93‑1 (MQ=255)

                                                      taTGATCTGGTAGGGCAGCTTTACTCCATGTTGTTCAACAATACTCATTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACAt                                   >  1:6998330/2‑93 (MQ=255)

                                                      cATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACAt                                   <  2:5963627/93‑1 (MQ=255)

                                                      cATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACAt                                   <  1:461074/93‑1 (MQ=255)

                                                          aaCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTgg                               <  2:7494784/93‑1 (MQ=255)

                                                          aaCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTgg                               >  1:1437827/1‑93 (MQ=255)

                                                           aCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGgc                              >  1:1373965/1‑93 (MQ=255)

                                                           aCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGgc                              <  1:2541460/93‑1 (MQ=255)

                                                           aCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGgc                              <  1:2398279/93‑1 (MQ=255)

                                                            cTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGCCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGgcc                             >  2:5115355/1‑92 (MQ=255)

                                                              ggTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCt                           >  2:3202520/1‑93 (MQ=255)

                                                              ggTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGGTCTACAGCCCAGTACTGGATACGCTGGACCATGGCACCGACCATGTGGCGCt                           >  2:5816787/1‑93 (MQ=255)

                                                              ggTAGGGCAGCCTTACTCCATGTTGTTAAACAATAGTCAGTTCTACAGTCCAGAACTGGATACGCGGGAACATGGCACGCAACATGTGgcgcg                           >  2:3826541/1‑92 (MQ=255)

                                                               gTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTg                          >  2:2841535/1‑93 (MQ=255)

                                                                 aGGGCAGCCTTACTCCATGGTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACAGGGCACCGAACATGTGGCGGTGga                        >  1:5387586/1‑93 (MQ=255)

                                                                  gggCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGgag                       >  1:5100855/1‑93 (MQ=255)

                                                                   ggCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGgaga                      >  2:5156769/1‑93 (MQ=255)

                                                                      aGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCa                   <  1:6426270/93‑1 (MQ=255)

                                                                      aGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCa                   <  1:2367413/93‑1 (MQ=255)

                                                                      aGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCa                   <  1:493442/93‑1 (MQ=255)

                                                                      aGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCa                   <  2:2575440/93‑1 (MQ=255)

                                                                       gCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAg                  <  2:4465873/93‑1 (MQ=255)

                                                                       gCCTTAATCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGTTCat                  >  2:7787306/1‑92 (MQ=255)

                                                                          ttACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGttt               >  1:7449549/1‑93 (MQ=255)

                                                                            aCTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTc             >  2:4742877/1‑93 (MQ=255)

                                                                            aCTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTc             <  2:879647/93‑1 (MQ=255)

                                                                             cTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTcc            >  1:4370992/1‑93 (MQ=255)

                                                                             cTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTcc            >  1:1203310/1‑93 (MQ=255)

                                                                              tCCCTGTTGTTCAACAATAATCTGTTCTACAGTACAGTACTGGATACGCTGGAACATGGCACCGTACATGTGGCGCTGGAGGTCAGTTTTCCa           <  1:7024486/93‑1 (MQ=255)

                                                                              tCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCa           <  2:6958426/93‑1 (MQ=255)

                                                                              tCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCa           >  1:109507/1‑93 (MQ=255)

                                                                               ccATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAg          >  1:5851041/1‑93 (MQ=255)

                                                                                cATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAgg         >  1:1099168/1‑93 (MQ=255)

                                                                                cATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAgg         >  2:5426581/1‑93 (MQ=255)

                                                                                cATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAgg         <  2:2315433/93‑1 (MQ=255)

                                                                                cATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAgg         >  1:5795435/1‑93 (MQ=255)

                                                                                  tgttgtTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTc       >  2:6559996/1‑93 (MQ=255)

                                                                                  tgttgtTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTc       <  2:2469523/93‑1 (MQ=255)

                                                                                  tgttgtTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTc       <  2:2188631/93‑1 (MQ=255)

                                                                                  tgttgtTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTc       >  2:1473576/1‑93 (MQ=255)

                                                                                   gttgttCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGCACATGTGGCGCTGGAGATCAGTTTTCCAGGTCg      >  1:7636469/1‑93 (MQ=255)

                                                                                      gttCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCGGGTCGTGa   >  1:4880419/1‑93 (MQ=255)

                                                                                      gttCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGa   <  2:2246459/93‑1 (MQ=255)

                                                                                       ttCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGAc  >  2:7222517/1‑93 (MQ=255)

GGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGAC  >  NC_000913/2318906‑2319085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCA  >  NC_000913/2318903‑2319089

CAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCA                                                                                               <  SRR4302543.2951433/94‑1 (MQ=60)

CAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCA                                                                                               >  SRR4302543.845904/1‑94 (MQ=60)

CAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCA                                                                                               >  SRR4302543.1195382/1‑94 (MQ=60)

 AGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAA                                                                                              >  SRR4302543.7271037/1‑94 (MQ=60)

 AGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGGTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAA                                                                                              >  SRR4302543.134988/1‑94 (MQ=60)

   GGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACA                                                                                            >  SRR4302543.7078700/1‑94 (MQ=60)

   GGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACA                                                                                            <  SRR4302543.1269225/94‑1 (MQ=60)

    GTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAA                                                                                           <  SRR4302543.5211314/94‑1 (MQ=60)

    GTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAA                                                                                           <  SRR4302543.5211315/94‑1 (MQ=60)

     TGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAAT                                                                                          <  SRR4302543.6408085/94‑1 (MQ=60)

     TGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAAT                                                                                          <  SRR4302543.6550588/94‑1 (MQ=60)

       ATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATAC                                                                                        >  SRR4302543.1404400/1‑94 (MQ=60)

       ATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATAC                                                                                        >  SRR4302543.7464835/1‑94 (MQ=60)

       ATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATAC                                                                                        <  SRR4302543.1643897/94‑1 (MQ=60)

       ATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATAC                                                                                        >  SRR4302543.3409262/1‑94 (MQ=60)

         GTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTC                                                                                      >  SRR4302543.5989318/1‑94 (MQ=60)

         GTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTC                                                                                      >  SRR4302543.466689/1‑94 (MQ=60)

         GTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTC                                                                                      >  SRR4302543.2922805/1‑94 (MQ=60)

          TAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCA                                                                                     <  SRR4302543.3477491/94‑1 (MQ=60)

           AACCACAATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAG                                                                                    <  SRR4302543.2943630/94‑1 (MQ=60)

            ACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGT                                                                                   >  SRR4302543.4138317/1‑94 (MQ=60)

             CCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTT                                                                                  >  SRR4302543.7992144/1‑94 (MQ=60)

              CACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTC                                                                                 <  SRR4302543.1958069/94‑1 (MQ=60)

              CACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTC                                                                                 <  SRR4302543.4637064/94‑1 (MQ=60)

                 CATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTAC                                                                              >  SRR4302543.6823704/1‑94 (MQ=60)

                   TGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAG                                                                            <  SRR4302543.6836606/94‑1 (MQ=60)

                      ACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCC                                                                         <  SRR4302543.5799019/94‑1 (MQ=60)

                       CCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTCGTTCAACAATACTCAGTTCTACAGTCCA                                                                        >  SRR4302543.4112355/1‑94 (MQ=60)

                        CCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAG                                                                       <  SRR4302543.1204740/94‑1 (MQ=60)

                          AGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTA                                                                     >  SRR4302543.2183464/1‑94 (MQ=60)

                          AGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTA                                                                     <  SRR4302543.6692762/94‑1 (MQ=60)

                           GCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTAC                                                                    <  SRR4302543.6851787/94‑1 (MQ=60)

                            CAACAGAAGTTATCACTGGCTAGCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACT                                                                   <  SRR4302543.1748129/94‑1 (MQ=60)

                             AGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTG                                                                  <  SRR4302543.6906968/94‑1 (MQ=60)

                              GCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGG                                                                 <  SRR4302543.6041968/94‑1 (MQ=60)

                              GCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGG                                                                 <  SRR4302543.6406867/94‑1 (MQ=60)

                              GCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGGAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGG                                                                 >  SRR4302543.4180154/1‑94 (MQ=60)

                                AGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGAT                                                               <  SRR4302543.5050037/94‑1 (MQ=60)

                                AGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGAT                                                               >  SRR4302543.3594919/1‑94 (MQ=60)

                                AGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGAT                                                               >  SRR4302543.98481/1‑94 (MQ=60)

                                AGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGAT                                                               >  SRR4302543.7908244/1‑94 (MQ=60)

                                AGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGAT                                                               <  SRR4302543.2903042/94‑1 (MQ=60)

                                   AGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACG                                                            >  SRR4302543.7338788/1‑94 (MQ=60)

                                   AGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACG                                                            <  SRR4302543.2703917/94‑1 (MQ=60)

                                   AGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACG                                                            >  SRR4302543.2706738/1‑94 (MQ=60)

                                    GTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTAgtggctaacc                                                           >  SRR4302543.4325178/1‑84 (MQ=60)

                                    GTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGC                                                           >  SRR4302543.3490481/1‑94 (MQ=60)

                                    GTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGC                                                           >  SRR4302543.5271274/1‑94 (MQ=60)

                                    GTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATAAGC                                                           >  SRR4302543.3490479/1‑94 (MQ=60)

                                      TATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTG                                                         >  SRR4302543.6801149/1‑94 (MQ=60)

                                      TATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTG                                                         <  SRR4302543.4972225/94‑1 (MQ=60)

                                        TCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGA                                                       >  SRR4302543.6519057/1‑94 (MQ=60)

                                         CACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAA                                                      <  SRR4302543.360676/94‑1 (MQ=60)

                                          ACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAC                                                     >  SRR4302543.5397845/1‑94 (MQ=60)

                                          ACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAC                                                     <  SRR4302543.5133871/94‑1 (MQ=60)

                                          ACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAC                                                     >  SRR4302543.2683324/1‑94 (MQ=60)

                                           CTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACA                                                    >  SRR4302543.3836189/1‑94 (MQ=60)

                                            TGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACAT                                                   <  SRR4302543.5971488/94‑1 (MQ=60)

                                            TGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACAT                                                   <  SRR4302543.2405850/94‑1 (MQ=60)

                                            TGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACAT                                                   <  SRR4302543.7507140/94‑1 (MQ=60)

                                             GGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATG                                                  >  SRR4302543.4452239/1‑94 (MQ=60)

                                               CTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGC                                                >  SRR4302543.1501837/1‑94 (MQ=60)

                                                TATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCA                                               <  SRR4302543.2332119/94‑1 (MQ=60)

                                                 ATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCAC                                              <  SRR4302543.2581538/94‑1 (MQ=60)

                                                 ATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCAC                                              >  SRR4302543.2221564/1‑94 (MQ=60)

                                                 ATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCAC                                              >  SRR4302543.5003020/1‑94 (MQ=60)

                                                  TCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACC                                             <  SRR4302543.4500743/94‑1 (MQ=60)

                                                    AACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGA                                           <  SRR4302543.5965005/94‑1 (MQ=60)

                                                      CGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAAC                                         >  SRR4302543.6730558/1‑94 (MQ=60)

                                                       GCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACA                                        >  SRR4302543.7792051/1‑94 (MQ=60)

                                                        CTATGATCTGGTAGGGCAGCTTTACTCCATGTTGTTCAACAATACTCATTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACAT                                       >  SRR4302543.7064491/1‑94 (MQ=60)

                                                        CCATGATCTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACAT                                       <  SRR4302543.5603497/94‑1 (MQ=60)

                                                         CATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATG                                      <  SRR4302543.267542/94‑1 (MQ=60)

                                                         CATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATG                                      <  SRR4302543.465367/94‑1 (MQ=60)

                                                          ATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGT                                     >  SRR4302543.2928560/1‑94 (MQ=60)

                                                            GAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGG                                   <  SRR4302543.3617958/94‑1 (MQ=60)

                                                            GAACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGG                                   >  SRR4302543.1450819/1‑94 (MQ=60)

                                                            GAACTCGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGG                                   <  SRR4302543.3214999/94‑1 (MQ=60)

                                                             AACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGC                                  >  SRR4302543.1386309/1‑94 (MQ=60)

                                                             AACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGC                                  <  SRR4302543.4337031/94‑1 (MQ=60)

                                                              ACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCG                                 <  SRR4302543.2564629/94‑1 (MQ=60)

                                                              ACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCG                                 <  SRR4302543.2419972/94‑1 (MQ=60)

                                                              ACTGGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCG                                 <  SRR4302543.2379240/94‑1 (MQ=60)

                                                                 GGTAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTG                              <  SRR4302543.541681/94‑1 (MQ=60)

                                                                   TAGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGA                            >  SRR4302543.5436533/1‑94 (MQ=60)

                                                                   TAGGGCAGCCTTACTCCATGGTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACAGGGCACCGAACATGTGGCGGTGGA                            >  SRR4302543.5436532/1‑94 (MQ=60)

                                                                    AGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAG                           <  SRR4302543.3524871/94‑1 (MQ=60)

                                                                    AGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAG                           >  SRR4302543.5147036/1‑94 (MQ=60)

                                                                    AGGGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAG                           <  SRR4302543.5000804/94‑1 (MQ=60)

                                                                      GGCAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGAT                         <  SRR4302543.6382665/94‑1 (MQ=60)

                                                                        CAGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCA                       <  SRR4302543.2794398/94‑1 (MQ=60)

                                                                         AGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAG                      <  SRR4302543.2388880/94‑1 (MQ=60)

                                                                         AGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAG                      <  SRR4302543.6486825/94‑1 (MQ=60)

                                                                         AGCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAG                      <  SRR4302543.498006/94‑1 (MQ=60)

                                                                          GCCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGT                     <  SRR4302543.5885803/94‑1 (MQ=60)

                                                                           CCTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTT                    >  SRR4302543.7552986/1‑94 (MQ=60)

                                                                            CTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTT                   >  SRR4302543.7441606/1‑94 (MQ=60)

                                                                            CTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTT                   >  SRR4302543.7520937/1‑94 (MQ=60)

                                                                            CTTACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTT                   >  SRR4302543.4400258/1‑94 (MQ=60)

                                                                              TACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTC                 <  SRR4302543.6414895/94‑1 (MQ=60)

                                                                               ACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCC                >  SRR4302543.6677345/1‑94 (MQ=60)

                                                                               ACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCC                >  SRR4302543.5380506/1‑94 (MQ=60)

                                                                               ACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCC                >  SRR4302543.4410577/1‑94 (MQ=60)

                                                                               ACTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCC                >  SRR4302543.1214146/1‑94 (MQ=60)

                                                                                CTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCA               >  SRR4302543.110508/1‑94 (MQ=60)

                                                                                CTCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCA               >  SRR4302543.110507/1‑94 (MQ=60)

                                                                                 TCCCTGTTGTTCAACAATAATCTGTTCTACAGTACAGTACTGGATACGCTGGAACATGGCACCGTACATGTGGCGCTGGAGGTCAGTTTTCCAG              <  SRR4302543.7091847/94‑1 (MQ=60)

                                                                                 TCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAG              <  SRR4302543.6566692/94‑1 (MQ=60)

                                                                                 TCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAG              >  SRR4302543.5904282/1‑94 (MQ=60)

                                                                                 TCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAG              <  SRR4302543.4870437/94‑1 (MQ=60)

                                                                                 TCCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAG              >  SRR4302543.2458561/1‑94 (MQ=60)

                                                                                  CCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGG             >  SRR4302543.5848162/1‑94 (MQ=60)

                                                                                  CCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGG             >  SRR4302543.183432/1‑94 (MQ=60)

                                                                                  CCATGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGG             >  SRR4302543.1109071/1‑94 (MQ=60)

                                                                                     TGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGCACATGTGGCGCTGGAGATCAGTTTTCCAGGTCG          >  SRR4302543.7708917/1‑94 (MQ=60)

                                                                                     TGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCG          <  SRR4302543.5929952/94‑1 (MQ=60)

                                                                                     TGTTGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCG          <  SRR4302543.135789/94‑1 (MQ=60)

                                                                                        TGTTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCGGGTCGTGA       >  SRR4302543.4924904/1‑94 (MQ=60)

                                                                                          TTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACC     <  SRR4302543.550521/94‑1 (MQ=60)

                                                                                          TTCAACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACC     >  SRR4302543.5052670/1‑94 (MQ=60)

                                                                                             AACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGGACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCA  >  SRR4302543.3407088/1‑94 (MQ=60)

CAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCA  >  NC_000913/2318903‑2319089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`